Restriction map for
pFN21AB8129
(Linear) (Six-base) MAPSORT of: KIBB8129.seq Check: 586 from: 1 to: 1023
KIBB8129.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 99 1023
Size: 99 924
AccI GT'mk_AC
Cuts at: 0 768 1023
Size: 768 255
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 517 1023
Size: 98 419 506
Fragments arranged by size:
506 419 98
AflIII A'CryG_T
Cuts at: 0 760 1023
Size: 760 263
AhdI GACnn_n'nnGTC
Cuts at: 0 764 1023
Size: 764 259
AlwNI CAG_nnn'CTG
Cuts at: 0 199 894 957 994 1023
Size: 199 695 63 37 29
Fragments arranged by size:
695 199 63 37 29
ApaLI G'TGCA_C
Cuts at: 0 315 1023
Size: 315 708
ApoI r'AATT_y
Cuts at: 0 541 1023
Size: 541 482
AvaI C'yCGr_G
Cuts at: 0 254 746 1023
Size: 254 492 277
Fragments arranged by size:
492 277 254
AvrII C'CTAG_G
Cuts at: 0 15 1023
Size: 15 1008
BamHI G'GATC_C
Cuts at: 0 11 1023
Size: 11 1012
BanII G_rGCy'C
Cuts at: 0 172 1023
Size: 172 851
BbsI GAAGACnn'nnnn_
Cuts at: 0 662 1023
Size: 662 361
BbvCI CC'TCA_GC
Cuts at: 0 511 1023
Size: 511 512
Bme1580I G_kGCm'C
Cuts at: 0 319 447 783 1023
Size: 319 128 336 240
Fragments arranged by size:
336 319 240 128
BmrI ACTGGGnnnn_n'
Cuts at: 0 499 1023
Size: 499 524
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1020 1023
Size: 1020 3
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 112 260 287 391 967 971 1023
Size: 88 24 148 27 104 576 4 52
Fragments arranged by size:
576 148 104 88 52 27 24 4
Bpu10I CC'TnA_GC
Cuts at: 0 511 1023
Size: 511 512
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 793 832 1023
Size: 793 39 191
Fragments arranged by size:
793 191 39
BsaAI yAC'GTr
Cuts at: 0 763 1023
Size: 763 260
BsaBI GATnn'nnATC
Cuts at: 0 43 1023
Size: 43 980
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 233 263 1023
Size: 233 30 760
Fragments arranged by size:
760 233 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 742 1023
Size: 742 281
BseYI C'CCAG_C
Cuts at: 0 435 479 928 1023
Size: 435 44 449 95
Fragments arranged by size:
449 435 95 44
BsiHKAI G_wGCw'C
Cuts at: 0 319 1023
Size: 319 704
BsmBI CGTCTCn'nnnn_
Cuts at: 0 397 1023
Size: 397 626
Bsp1286I G_dGCh'C
Cuts at: 0 172 319 447 783 1023
Size: 172 147 128 336 240
Fragments arranged by size:
336 240 172 147 128
BspHI T'CATG_A
Cuts at: 0 665 1023
Size: 665 358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 99 1023
Size: 99 924
BsrDI GCAATG_nn'
Cuts at: 0 260 1023
Size: 260 763
BstAPI GCAn_nnn'nTGC
Cuts at: 0 894 957 1023
Size: 894 63 66
Fragments arranged by size:
894 66 63
BstEII G'GTnAC_C
Cuts at: 0 639 1023
Size: 639 384
BstXI CCAn_nnnn'nTGG
Cuts at: 0 651 1023
Size: 651 372
BstYI r'GATC_y
Cuts at: 0 11 38 1023
Size: 11 27 985
Fragments arranged by size:
985 27 11
BtgI C'CryG_G
Cuts at: 0 48 650 1023
Size: 48 602 373
Fragments arranged by size:
602 373 48
DraIII CAC_nnn'GTG
Cuts at: 0 493 1023
Size: 493 530
EaeI y'GGCC_r
Cuts at: 0 201 836 1023
Size: 201 635 187
Fragments arranged by size:
635 201 187
EarI CTCTTCn'nnn_
Cuts at: 0 904 1023
Size: 904 119
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 906 1023
Size: 906 117
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 98 112 260 287 391 517 967
Size: 88 10 14 148 27 104 126 450
Cuts at: 967 971 1023
Size: 4 52
Fragments arranged by size:
450 148 126 104 88 52 27 14
10 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 19 106 1023
Size: 19 87 917
Fragments arranged by size:
917 87 19
EcoO109I rG'GnC_Cy
Cuts at: 0 99 124 742 1023
Size: 99 25 618 281
Fragments arranged by size:
618 281 99 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 116 148 750 782 1023
Size: 116 32 602 32 241
Fragments arranged by size:
602 241 116 32 32
HincII GTy'rAC
Cuts at: 0 769 1023
Size: 769 254
MscI TGG'CCA
Cuts at: 0 838 1023
Size: 838 185
MslI CAynn'nnrTG
Cuts at: 0 664 670 1023
Size: 664 6 353
Fragments arranged by size:
664 353 6
MspA1I CmG'CkG
Cuts at: 0 435 891 945 1023
Size: 435 456 54 78
Fragments arranged by size:
456 435 78 54
NcoI C'CATG_G
Cuts at: 0 650 1023
Size: 650 373
NspI r_CATG'y
Cuts at: 0 487 1023
Size: 487 536
PfoI T'CCnGG_A
Cuts at: 0 854 987 1023
Size: 854 133 36
Fragments arranged by size:
854 133 36
PmlI CAC'GTG
Cuts at: 0 763 1023
Size: 763 260
PpuMI rG'GwC_Cy
Cuts at: 0 99 124 1023
Size: 99 25 899
Fragments arranged by size:
899 99 25
PstI C_TGCA'G
Cuts at: 0 386 434 476 962 1023
Size: 386 48 42 486 61
Fragments arranged by size:
486 386 61 48 42
PvuII CAG'CTG
Cuts at: 0 435 891 945 1023
Size: 435 456 54 78
Fragments arranged by size:
456 435 78 54
SalI G'TCGA_C
Cuts at: 0 767 1023
Size: 767 256
SapI GCTCTTCn'nnn_
Cuts at: 0 904 1023
Size: 904 119
SfcI C'TryA_G
Cuts at: 0 382 430 462 472 958 1023
Size: 382 48 32 10 486 65
Fragments arranged by size:
486 382 65 48 32 10
SmaI CCC'GGG
Cuts at: 0 748 1023
Size: 748 275
SmlI C'TyrA_G
Cuts at: 0 772 847 1023
Size: 772 75 176
Fragments arranged by size:
772 176 75
SphI G_CATG'C
Cuts at: 0 487 1023
Size: 487 536
StyI C'CwwG_G
Cuts at: 0 15 650 1023
Size: 15 635 373
Fragments arranged by size:
635 373 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 481 1023
Size: 481 542
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 233 265 1023
Size: 233 32 758
Fragments arranged by size:
758 233 32
Tth111I GACn'n_nGTC
Cuts at: 0 283 1023
Size: 283 740
XmaI C'CCGG_G
Cuts at: 0 746 1023
Size: 746 277
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AlwNI ApaLI ApoI
AvaI AvrII BamHI BanII BbsI BbvCI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaXI BseRI
BseYI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI BstAPI
BstEII BstXI BstYI BtgI DraIII EaeI EarI EciI
Eco57MI EcoNI EcoO109I Hin4I HincII MscI MslI MspA1I
NcoI NspI PfoI PmlI PpuMI PstI PvuII SalI
SapI SfcI SmaI SmlI SphI StyI TaqII TstI
Tth111I XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AleI AlfI
AloI ApaI AscI AseI AsiSI BaeI BanI BbeI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI
BmtI BsaI BsaHI BsaWI BsgI BsiEI BsiWI BsmI
BspEI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EagI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SrfI SspI StuI SwaI TatI XbaI XcmI XhoI
XmnI ZraI