(Circular) (Six-base) MAPSORT of: pFN21AB8126.seq Check: 9939 from: 1 to: 6364 pFN21AB8126 6364 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4174 278 Size: 53 83 186 936 2638 2468 Fragments arranged by size: 2638 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3707 1937 Size: 1770 4594 Acc65I G'GTAC_C Cuts at: 3296 3685 3296 Size: 389 5975 AclI AA'CG_TT Cuts at: 1563 2695 3231 4491 4864 1563 Size: 1132 536 1260 373 3063 Fragments arranged by size: 3063 1260 1132 536 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2286 2843 3437 4427 5811 6079 1632 Size: 654 557 594 990 1384 268 1917 Fragments arranged by size: 1917 1384 990 654 594 557 268 AflII C'TTAA_G Cuts at: 820 1017 2227 820 Size: 197 1210 4957 Fragments arranged by size: 4957 1210 197 AflIII A'CryG_T Cuts at: 1203 2507 5263 6240 1203 Size: 1304 2756 977 1327 Fragments arranged by size: 2756 1327 1304 977 AgeI A'CCGG_T Cuts at: 5195 5195 Size: 6364 AhdI GACnn_n'nnGTC Cuts at: 5093 5093 Size: 6364 AleI CACnn'nnGTG Cuts at: 1166 2822 1166 Size: 1656 4708 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6364 AlwNI CAG_nnn'CTG Cuts at: 2866 4107 5679 2866 Size: 1241 1572 3551 Fragments arranged by size: 3551 1572 1241 ApaLI G'TGCA_C Cuts at: 4421 5577 4421 Size: 1156 5208 ApoI r'AATT_y Cuts at: 1493 2550 3640 3673 3899 4051 6160 1493 Size: 1057 1090 33 226 152 2109 1697 Fragments arranged by size: 2109 1697 1090 1057 226 152 33 AseI AT'TA_AT Cuts at: 160 1032 4918 160 Size: 872 3886 1606 Fragments arranged by size: 3886 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6364 AvaI C'yCGr_G Cuts at: 1870 1943 3689 1870 Size: 73 1746 4545 Fragments arranged by size: 4545 1746 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2184 2217 2846 2879 3196 3229 2184 Size: 33 629 33 317 33 5319 Fragments arranged by size: 5319 629 317 33 33 33 BamHI G'GATC_C Cuts at: 3257 3694 4061 3257 Size: 437 367 5560 Fragments arranged by size: 5560 437 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2602 3296 3685 5140 618 Size: 325 217 627 815 694 389 1455 1842 Fragments arranged by size: 1842 1455 815 694 627 389 325 217 BanII G_rGCy'C Cuts at: 1391 3507 3683 1391 Size: 2116 176 4072 Fragments arranged by size: 4072 2116 176 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5393 BbvCI CC'TCA_GC Cuts at: 2264 2264 Size: 6364 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4555 4589 1903 Size: 34 50 34 2534 34 3678 Fragments arranged by size: 3678 2534 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3224 4256 5466 3224 Size: 1032 1210 4122 Fragments arranged by size: 4122 1210 1032 BclI T'GATC_A Cuts at: 1548 2928 1548 Size: 1380 4984 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4975 136 Size: 107 122 71 631 605 3303 1525 Fragments arranged by size: 3303 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3180 3597 6359 3180 Size: 417 2762 3185 Fragments arranged by size: 3185 2762 417 BlpI GC'TnA_GC Cuts at: 3781 3781 Size: 6364 Bme1580I G_kGCm'C Cuts at: 1790 3578 4425 5581 1790 Size: 1788 847 1156 2573 Fragments arranged by size: 2573 1788 1156 847 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6364 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2151 2226 2661 Size: 416 53 83 399 24 734 75 435 Cuts at: 2661 2770 3340 4095 5053 442 Size: 109 570 755 958 1753 Fragments arranged by size: 1753 958 755 734 570 435 416 399 109 83 75 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6364 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2176 2176 Size: 6364 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2053 3071 3521 5024 1239 Size: 814 1018 450 1503 2579 Fragments arranged by size: 2579 1503 1018 814 450 Bpu10I CC'TnA_GC Cuts at: 2264 2264 Size: 6364 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4489 5354 5652 4489 Size: 865 298 5201 Fragments arranged by size: 5201 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2071 5027 882 Size: 617 572 2956 2219 Fragments arranged by size: 2956 2219 617 572 BsaAI yAC'GTr Cuts at: 493 1204 4155 493 Size: 711 2951 2702 Fragments arranged by size: 2951 2702 711 BsaBI GATnn'nnATC Cuts at: 6358 6358 Size: 6364 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4171 4553 5188 275 Size: 53 83 186 936 2638 382 635 1451 Fragments arranged by size: 2638 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4797 5195 5469 5616 6027 4797 Size: 398 274 147 411 5134 Fragments arranged by size: 5134 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2379 2400 2409 2430 3023 Size: 30 165 30 976 21 9 21 593 Cuts at: 3023 3053 1178 Size: 30 4489 Fragments arranged by size: 4489 976 593 165 30 30 30 21 21 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2450 1186 Size: 1264 5100 BseYI C'CCAG_C Cuts at: 3506 5567 6063 3506 Size: 2061 496 3807 Fragments arranged by size: 3807 2061 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5791 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2031 3814 4575 5603 1540 Size: 275 186 30 1783 761 1028 2301 Fragments arranged by size: 2301 1783 1028 761 275 186 30 BsiHKAI G_wGCw'C Cuts at: 4425 4510 5581 4425 Size: 85 1071 5208 Fragments arranged by size: 5208 1071 85 BsmI GAATG_Cn' Cuts at: 3535 3875 3968 3535 Size: 340 93 5931 Fragments arranged by size: 5931 340 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3507 3578 3683 4425 4510 5581 1391 Size: 399 1717 71 105 742 85 1071 2174 Fragments arranged by size: 2174 1717 1071 742 399 105 85 71 BspEI T'CCGG_A Cuts at: 6027 6027 Size: 6364 BspHI T'CATG_A Cuts at: 4253 5983 4253 Size: 1730 4634 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3277 3284 3719 844 Size: 2433 7 435 3489 Fragments arranged by size: 3489 2433 435 7 BsrBI CCG'CTC Cuts at: 1394 1823 4251 1394 Size: 429 2428 3507 Fragments arranged by size: 3507 2428 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2094 4859 5033 65 Size: 1183 846 2765 174 1396 Fragments arranged by size: 2765 1396 1183 846 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5008 5195 1700 Size: 110 3198 187 2869 Fragments arranged by size: 3198 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6364 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6364 BssSI C'ACGA_G Cuts at: 3249 4424 5436 3249 Size: 1175 1012 4177 Fragments arranged by size: 4177 1175 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6364 BstBI TT'CG_AA Cuts at: 5168 5168 Size: 6364 BstEII G'GTnAC_C Cuts at: 1184 2639 1184 Size: 1455 4909 BstXI CCAn_nnnn'nTGG Cuts at: 3307 3307 Size: 6364 BstYI r'GATC_y Cuts at: 1972 2708 2762 3180 3257 3597 3694 4061 4446 Size: 736 54 418 77 340 97 367 385 Cuts at: 4446 4463 5915 6001 6013 6359 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 736 418 385 367 346 340 97 86 77 54 17 12 BtgI C'CryG_G Cuts at: 513 1065 2031 2889 3438 513 Size: 552 966 858 549 3439 Fragments arranged by size: 3439 966 858 552 549 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5625 Fragments arranged by size: 5625 683 56 BtsI GCAGTG_nn' Cuts at: 3880 4674 4694 3880 Size: 794 20 5550 Fragments arranged by size: 5550 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6364 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6329 DraI TTT'AAA Cuts at: 3668 3668 Size: 6364 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3000 5371 809 Size: 507 1684 2371 1802 Fragments arranged by size: 2371 1802 1684 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 3811 4700 Size: 54 1425 50 275 18 198 1783 889 Cuts at: 4700 6329 8 Size: 1629 43 Fragments arranged by size: 1783 1629 1425 889 275 198 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2028 3811 1537 Size: 275 216 1783 4090 Fragments arranged by size: 4090 1783 275 216 EarI CTCTTCn'nnn_ Cuts at: 1353 2166 2393 2780 4294 1353 Size: 813 227 387 1514 3423 Fragments arranged by size: 3423 1514 813 387 227 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2978 3206 4951 5325 5471 387 Size: 1064 138 1389 228 1745 374 146 1280 Fragments arranged by size: 1745 1389 1280 1064 374 228 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2053 2286 2843 3071 3437 3521 4427 Size: 393 421 233 557 228 366 84 906 Cuts at: 4427 5024 5811 6079 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 906 787 597 557 421 393 366 268 233 228 84 EcoRI G'AATT_C Cuts at: 3673 4051 3673 Size: 378 5986 EcoRV GAT'ATC Cuts at: 3488 3488 Size: 6364 FspI TGC'GCA Cuts at: 4870 4870 Size: 6364 HaeII r_GCGC'y Cuts at: 1678 5511 1678 Size: 3833 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2930 2962 5005 5037 5079 Size: 32 135 32 1358 32 2043 32 42 Cuts at: 5079 5111 1373 Size: 32 2626 Fragments arranged by size: 2626 2043 1358 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3708 3954 669 Size: 988 281 1770 246 3079 Fragments arranged by size: 3079 1770 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6364 HpaI GTT'AAC Cuts at: 3954 3954 Size: 6364 KpnI G_GTAC'C Cuts at: 3300 3689 3300 Size: 389 5975 MfeI C'AATT_G Cuts at: 2493 2607 2664 3963 2493 Size: 114 57 1299 4894 Fragments arranged by size: 4894 1299 114 57 MluI A'CGCG_T Cuts at: 2507 6240 2507 Size: 3733 2631 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3173 3235 5478 5662 1305 Size: 1868 62 2243 184 2007 Fragments arranged by size: 2243 2007 1868 184 62 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4542 Fragments arranged by size: 4542 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2822 2825 4322 4681 4840 518 Size: 648 1656 3 1497 359 159 2042 Fragments arranged by size: 2042 1656 1497 648 359 159 3 MspA1I CmG'CkG Cuts at: 1606 3263 3780 4457 5605 5850 1606 Size: 1657 517 677 1148 245 2120 Fragments arranged by size: 2120 1657 1148 677 517 245 NcoI C'CATG_G Cuts at: 513 1065 2889 3438 513 Size: 552 1824 549 3439 Fragments arranged by size: 3439 1824 552 549 NdeI CA'TA_TG Cuts at: 387 387 Size: 6364 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6364 NotI GC'GGCC_GC Cuts at: 3811 3811 Size: 6364 NspI r_CATG'y Cuts at: 1132 1229 2924 3722 5267 1132 Size: 97 1695 798 1545 2229 Fragments arranged by size: 2229 1695 1545 798 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6364 PciI A'CATG_T Cuts at: 5263 5263 Size: 6364 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6364 PmeI GTTT'AAAC Cuts at: 3668 3668 Size: 6364 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4433 4465 5946 5978 4433 Size: 32 1481 32 4819 Fragments arranged by size: 4819 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6364 PsiI TTA'TAA Cuts at: 3934 3934 Size: 6364 PstI C_TGCA'G Cuts at: 830 3716 830 Size: 2886 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6364 SalI G'TCGA_C Cuts at: 1936 3706 1936 Size: 1770 4594 SapI GCTCTTCn'nnn_ Cuts at: 2166 2166 Size: 6364 SbfI CC_TGCA'GG Cuts at: 3716 3716 Size: 6364 ScaI AGT'ACT Cuts at: 4612 6324 4612 Size: 1712 4652 SfcI C'TryA_G Cuts at: 826 1045 3712 4847 5528 5719 826 Size: 219 2667 1135 681 191 1471 Fragments arranged by size: 2667 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6364 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6364 SmaI CCC'GGG Cuts at: 1872 3691 1872 Size: 1819 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2227 4468 5369 5631 820 Size: 197 926 284 2241 901 262 1553 Fragments arranged by size: 2241 1553 926 901 284 262 197 SnaBI TAC'GTA Cuts at: 493 4155 493 Size: 3662 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6364 SphI G_CATG'C Cuts at: 1132 3722 1132 Size: 2590 3774 SspI AAT'ATT Cuts at: 5 52 4288 5 Size: 47 4236 2081 Fragments arranged by size: 4236 2081 47 StuI AGG'CCT Cuts at: 1517 3011 1517 Size: 1494 4870 StyI C'CwwG_G Cuts at: 513 1065 2464 2889 3438 3803 513 Size: 552 1399 425 549 365 3074 Fragments arranged by size: 3074 1399 552 549 425 365 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2658 4391 4408 4561 4746 5191 325 Size: 1255 1078 1733 17 153 185 445 1498 Fragments arranged by size: 1733 1498 1255 1078 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2730 2814 4610 Size: 274 80 33 51 1443 753 84 1796 Cuts at: 4610 6322 96 Size: 1712 138 Fragments arranged by size: 1796 1712 1443 753 274 138 84 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2379 2400 2411 2432 6324 6356 2379 Size: 21 11 21 3892 32 2387 Fragments arranged by size: 3892 2387 32 21 21 11 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6364 XbaI T'CTAG_A Cuts at: 3700 3700 Size: 6364 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2077 2077 Size: 6364 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6364 XmaI C'CCGG_G Cuts at: 1870 3689 1870 Size: 1819 4545 XmnI GAAnn'nnTTC Cuts at: 2696 3533 4493 2696 Size: 837 960 4567 Fragments arranged by size: 4567 960 837 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4172 276 Size: 53 83 186 936 2638 2468 Fragments arranged by size: 2638 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI