(Circular) (Six-base) MAPSORT of: pFN21AB8113.seq Check: 4526 from: 1 to: 5845 pFN21AB8113 5845 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2284 2792 2284 Size: 508 5337 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3655 278 Size: 53 83 186 936 2119 2468 Fragments arranged by size: 2468 2119 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2100 3188 1937 Size: 163 1088 4594 Fragments arranged by size: 4594 1088 163 Acc65I G'GTAC_C Cuts at: 3166 3166 Size: 5845 AclI AA'CG_TT Cuts at: 1563 3972 4345 1563 Size: 2409 373 3063 Fragments arranged by size: 3063 2409 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2572 3908 5292 5560 1632 Size: 940 1336 1384 268 1917 Fragments arranged by size: 1917 1384 1336 940 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5648 AflIII A'CryG_T Cuts at: 1203 2095 4744 5721 1203 Size: 892 2649 977 1327 Fragments arranged by size: 2649 1327 977 892 AgeI A'CCGG_T Cuts at: 4676 4676 Size: 5845 AhdI GACnn_n'nnGTC Cuts at: 4574 4574 Size: 5845 AleI CACnn'nnGTG Cuts at: 1166 2870 1166 Size: 1704 4141 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5845 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2619 2651 2619 Size: 32 5813 AlwNI CAG_nnn'CTG Cuts at: 2947 3588 5160 2947 Size: 641 1572 3632 Fragments arranged by size: 3632 1572 641 ApaLI G'TGCA_C Cuts at: 3902 5058 3902 Size: 1156 4689 ApoI r'AATT_y Cuts at: 1493 3154 3380 3532 5641 1493 Size: 1661 226 152 2109 1697 Fragments arranged by size: 2109 1697 1661 226 152 AseI AT'TA_AT Cuts at: 160 1032 4399 160 Size: 872 3367 1606 Fragments arranged by size: 3367 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5845 AvaI C'yCGr_G Cuts at: 1870 1943 3170 1870 Size: 73 1227 4545 Fragments arranged by size: 4545 1227 73 BamHI G'GATC_C Cuts at: 2853 3175 3542 2853 Size: 322 367 5156 Fragments arranged by size: 5156 367 322 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2236 3166 4621 618 Size: 325 217 627 449 930 1455 1842 Fragments arranged by size: 1842 1455 930 627 449 325 217 BanII G_rGCy'C Cuts at: 1391 2331 2889 3050 3164 1391 Size: 940 558 161 114 4072 Fragments arranged by size: 4072 940 558 161 114 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2088 2797 928 Size: 971 189 709 3976 Fragments arranged by size: 3976 971 709 189 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4036 4070 1903 Size: 34 50 34 2015 34 3678 Fragments arranged by size: 3678 2015 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3737 4947 3737 Size: 1210 4635 BclI T'GATC_A Cuts at: 1548 1548 Size: 5845 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2456 4456 136 Size: 107 122 71 631 605 784 2000 1525 Fragments arranged by size: 2000 1525 784 631 605 122 107 71 BglII A'GATC_T Cuts at: 2116 5840 2116 Size: 3724 2121 BlpI GC'TnA_GC Cuts at: 3262 3262 Size: 5845 Bme1580I G_kGCm'C Cuts at: 1790 2239 3906 5062 1790 Size: 449 1667 1156 2573 Fragments arranged by size: 2573 1667 1156 449 BmgBI CAC'GTC Cuts at: 1318 2096 1318 Size: 778 5067 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2379 2533 3576 Size: 416 53 83 399 24 962 154 1043 Cuts at: 3576 4534 442 Size: 958 1753 Fragments arranged by size: 1753 1043 962 958 416 399 154 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5845 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1994 2437 2762 2903 4505 1239 Size: 755 443 325 141 1602 2579 Fragments arranged by size: 2579 1602 755 443 325 141 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2378 3970 4835 5133 2378 Size: 1592 865 298 3090 Fragments arranged by size: 3090 1592 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4508 882 Size: 617 3009 2219 Fragments arranged by size: 3009 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2182 3636 493 Size: 711 978 1454 2702 Fragments arranged by size: 2702 1454 978 711 BsaBI GATnn'nnATC Cuts at: 5839 5839 Size: 5845 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3652 4034 4669 275 Size: 53 83 186 936 2119 382 635 1451 Fragments arranged by size: 2119 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4278 4676 4950 5097 5508 4278 Size: 398 274 147 411 4615 Fragments arranged by size: 4615 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5620 Fragments arranged by size: 5620 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2381 3074 3093 1186 Size: 1195 693 19 3938 Fragments arranged by size: 3938 1195 693 19 BseYI C'CCAG_C Cuts at: 2696 3071 5048 5544 2696 Size: 375 1977 496 2997 Fragments arranged by size: 2997 1977 496 375 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5272 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3295 4056 5084 1540 Size: 275 186 1294 761 1028 2301 Fragments arranged by size: 2301 1294 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2528 2889 3050 3906 3991 5062 2528 Size: 361 161 856 85 1071 3311 Fragments arranged by size: 3311 1071 856 361 161 85 BsmI GAATG_Cn' Cuts at: 3356 3449 3356 Size: 93 5752 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2239 2331 2528 2889 3050 3164 3906 Size: 399 449 92 197 361 161 114 742 Cuts at: 3906 3991 5062 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 449 399 361 197 161 114 92 85 BspEI T'CCGG_A Cuts at: 5508 5508 Size: 5845 BspHI T'CATG_A Cuts at: 3734 5464 3734 Size: 1730 4115 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2284 2745 2792 3200 844 Size: 1440 461 47 408 3489 Fragments arranged by size: 3489 1440 461 408 47 BsrBI CCG'CTC Cuts at: 1394 1823 2325 3732 1394 Size: 429 502 1407 3507 Fragments arranged by size: 3507 1407 502 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2445 2592 2860 4340 4514 65 Size: 1183 1197 147 268 1480 174 1396 Fragments arranged by size: 1480 1396 1197 1183 268 174 147 BsrFI r'CCGG_y Cuts at: 1700 1810 2379 2450 3090 4489 4676 1700 Size: 110 569 71 640 1399 187 2869 Fragments arranged by size: 2869 1399 640 569 187 110 71 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5845 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5845 BssSI C'ACGA_G Cuts at: 3905 4917 3905 Size: 1012 4833 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5845 BstBI TT'CG_AA Cuts at: 4649 4649 Size: 5845 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5845 BstXI CCAn_nnnn'nTGG Cuts at: 2296 2296 Size: 5845 BstYI r'GATC_y Cuts at: 1972 2116 2853 3175 3542 3927 3944 5396 5482 Size: 144 737 322 367 385 17 1452 86 Cuts at: 5482 5494 5840 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 737 385 367 346 322 144 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5293 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5106 Fragments arranged by size: 5106 683 56 BtsI GCAGTG_nn' Cuts at: 3031 3361 4155 4175 3031 Size: 330 794 20 4701 Fragments arranged by size: 4701 794 330 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5845 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5810 DraI TTT'AAA Cuts at: 3149 3149 Size: 5845 DraIII CAC_nnn'GTG Cuts at: 2644 2644 Size: 5845 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4852 809 Size: 507 3536 1802 Fragments arranged by size: 3536 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2335 2457 2646 Size: 54 1425 50 275 18 505 122 189 Cuts at: 2646 3106 3292 4181 5810 8 Size: 460 186 889 1629 43 Fragments arranged by size: 1629 1425 889 505 460 275 189 186 122 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3292 1537 Size: 275 1480 4090 Fragments arranged by size: 4090 1480 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2153 2591 2731 3775 1353 Size: 800 438 140 1044 3423 Fragments arranged by size: 3423 1044 800 438 140 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4432 4806 4952 387 Size: 1064 138 2843 374 146 1280 Fragments arranged by size: 2843 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2887 3048 2887 Size: 161 5684 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1994 2437 2572 2762 2903 3908 4505 Size: 393 362 443 135 190 141 1005 597 Cuts at: 4505 5292 5560 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1005 787 597 443 393 362 268 190 141 135 EcoRI G'AATT_C Cuts at: 3154 3532 3154 Size: 378 5467 EcoRV GAT'ATC Cuts at: 2513 2513 Size: 5845 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2324 2324 Size: 5845 FspI TGC'GCA Cuts at: 2978 4351 2978 Size: 1373 4472 HaeII r_GCGC'y Cuts at: 1678 4992 1678 Size: 3314 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2297 2329 2823 2855 4486 Size: 32 135 32 725 32 494 32 1631 Cuts at: 4486 4518 4560 4592 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1631 725 494 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3189 3435 669 Size: 988 281 1251 246 3079 Fragments arranged by size: 3079 1251 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5845 HpaI GTT'AAC Cuts at: 3435 3435 Size: 5845 KpnI G_GTAC'C Cuts at: 3170 3170 Size: 5845 MfeI C'AATT_G Cuts at: 3444 3444 Size: 5845 MluI A'CGCG_T Cuts at: 5721 5721 Size: 5845 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4959 5143 1305 Size: 3654 184 2007 Fragments arranged by size: 3654 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2648 3108 10 Size: 54 1425 343 816 460 2747 Fragments arranged by size: 2747 1425 816 460 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2870 3803 4162 4321 518 Size: 648 1704 933 359 159 2042 Fragments arranged by size: 2042 1704 933 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2415 2944 3071 3261 3938 5086 5331 1606 Size: 809 529 127 190 677 1148 245 2120 Fragments arranged by size: 2120 1148 809 677 529 245 190 127 NaeI GCC'GGC Cuts at: 2452 3092 2452 Size: 640 5205 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5293 NdeI CA'TA_TG Cuts at: 387 387 Size: 5845 NgoMIV G'CCGG_C Cuts at: 2450 3090 2450 Size: 640 5205 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5845 NotI GC'GGCC_GC Cuts at: 3292 3292 Size: 5845 NspI r_CATG'y Cuts at: 1132 1229 3203 4748 1132 Size: 97 1974 1545 2229 Fragments arranged by size: 2229 1974 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5845 PciI A'CATG_T Cuts at: 4744 4744 Size: 5845 PflMI CCAn_nnn'nTGG Cuts at: 1108 2644 1108 Size: 1536 4309 PmeI GTTT'AAAC Cuts at: 3149 3149 Size: 5845 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2339 2371 3914 3946 5427 5459 2339 Size: 32 1543 32 1481 32 2725 Fragments arranged by size: 2725 1543 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5845 PsiI TTA'TAA Cuts at: 3415 3415 Size: 5845 PstI C_TGCA'G Cuts at: 830 3070 3197 830 Size: 2240 127 3478 Fragments arranged by size: 3478 2240 127 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5845 PvuII CAG'CTG Cuts at: 2415 2944 3071 2415 Size: 529 127 5189 Fragments arranged by size: 5189 529 127 SacI G_AGCT'C Cuts at: 2889 3050 2889 Size: 161 5684 SalI G'TCGA_C Cuts at: 1936 3187 1936 Size: 1251 4594 SapI GCTCTTCn'nnn_ Cuts at: 2153 2591 2153 Size: 438 5407 SbfI CC_TGCA'GG Cuts at: 3197 3197 Size: 5845 ScaI AGT'ACT Cuts at: 4093 5805 4093 Size: 1712 4133 SfcI C'TryA_G Cuts at: 826 1045 2939 3066 3193 4328 5009 5200 826 Size: 219 1894 127 127 1135 681 191 1471 Fragments arranged by size: 1894 1471 1135 681 219 191 127 127 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5845 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5845 SmaI CCC'GGG Cuts at: 1872 3172 1872 Size: 1300 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2393 3949 4850 5112 820 Size: 197 926 450 1556 901 262 1553 Fragments arranged by size: 1556 1553 926 901 450 262 197 SnaBI TAC'GTA Cuts at: 493 3636 493 Size: 3143 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5845 SphI G_CATG'C Cuts at: 1132 3203 1132 Size: 2071 3774 SspI AAT'ATT Cuts at: 5 52 3769 5 Size: 47 3717 2081 Fragments arranged by size: 3717 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5845 StyI C'CwwG_G Cuts at: 513 1065 2253 3284 513 Size: 552 1188 1031 3074 Fragments arranged by size: 3074 1188 1031 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2100 3872 3889 4042 4227 4672 325 Size: 1255 520 1772 17 153 185 445 1498 Fragments arranged by size: 1772 1498 1255 520 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4091 5803 96 Size: 274 80 33 51 1443 2114 1712 138 Fragments arranged by size: 2114 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2030 2062 5805 5837 2030 Size: 32 3743 32 2038 Fragments arranged by size: 3743 2038 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5845 XbaI T'CTAG_A Cuts at: 2077 3181 2077 Size: 1104 4741 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2251 2251 Size: 5845 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5845 XmaI C'CCGG_G Cuts at: 1870 3170 1870 Size: 1300 4545 XmnI GAAnn'nnTTC Cuts at: 3974 3974 Size: 5845 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3653 276 Size: 53 83 186 936 2119 2468 Fragments arranged by size: 2468 2119 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoO109I FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI