(Circular) (Six-base) MAPSORT of: pFN21AB8111.seq Check: 4567 from: 1 to: 6373 pFN21AB8111 6373 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3154 3154 Size: 6373 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4183 278 Size: 53 83 186 936 2647 2468 Fragments arranged by size: 2647 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3716 1937 Size: 1779 4594 Acc65I G'GTAC_C Cuts at: 3694 3694 Size: 6373 AclI AA'CG_TT Cuts at: 1563 4500 4873 1563 Size: 2937 373 3063 Fragments arranged by size: 3063 2937 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4436 5820 6088 1632 Size: 2804 1384 268 1917 Fragments arranged by size: 2804 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6176 AflIII A'CryG_T Cuts at: 1203 5272 6249 1203 Size: 4069 977 1327 Fragments arranged by size: 4069 1327 977 AgeI A'CCGG_T Cuts at: 5204 5204 Size: 6373 AhdI GACnn_n'nnGTC Cuts at: 5102 5102 Size: 6373 AleI CACnn'nnGTG Cuts at: 1166 3604 1166 Size: 2438 3935 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6373 AlwNI CAG_nnn'CTG Cuts at: 4116 5688 4116 Size: 1572 4801 ApaI G_GGCC'C Cuts at: 2150 3389 2150 Size: 1239 5134 ApaLI G'TGCA_C Cuts at: 4430 5586 4430 Size: 1156 5217 ApoI r'AATT_y Cuts at: 1493 2337 2915 3495 3532 3682 3908 4060 6169 Size: 844 578 580 37 150 226 152 2109 Cuts at: 6169 1493 Size: 1697 Fragments arranged by size: 2109 1697 844 580 578 226 152 150 37 AseI AT'TA_AT Cuts at: 160 1032 4927 160 Size: 872 3895 1606 Fragments arranged by size: 3895 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6373 AvaI C'yCGr_G Cuts at: 1870 1943 2301 3698 1870 Size: 73 358 1397 4545 Fragments arranged by size: 4545 1397 358 73 BamHI G'GATC_C Cuts at: 3466 3703 4070 3466 Size: 237 367 5769 Fragments arranged by size: 5769 367 237 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2186 3348 3694 5149 618 Size: 325 217 627 399 1162 346 1455 1842 Fragments arranged by size: 1842 1455 1162 627 399 346 325 217 BanII G_rGCy'C Cuts at: 1391 2150 2806 3389 3692 1391 Size: 759 656 583 303 4072 Fragments arranged by size: 4072 759 656 583 303 BbeI G_GCGC'C Cuts at: 2190 2190 Size: 6373 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3111 3162 928 Size: 971 1212 51 4139 Fragments arranged by size: 4139 1212 971 51 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4564 4598 1903 Size: 34 2627 34 3678 Fragments arranged by size: 3678 2627 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2993 4265 5475 2993 Size: 1272 1210 3891 Fragments arranged by size: 3891 1272 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6373 BfrBI ATG'CAT Cuts at: 2814 2814 Size: 6373 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3153 4984 136 Size: 107 122 71 631 605 1481 1831 1525 Fragments arranged by size: 1831 1525 1481 631 605 122 107 71 BglII A'GATC_T Cuts at: 6368 6368 Size: 6373 BlpI GC'TnA_GC Cuts at: 3790 3790 Size: 6373 Bme1580I G_kGCm'C Cuts at: 1790 2043 2150 3389 4434 5590 1790 Size: 253 107 1239 1045 1156 2573 Fragments arranged by size: 2573 1239 1156 1045 253 107 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6373 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3383 4104 5062 Size: 416 53 83 399 24 1966 721 958 Cuts at: 5062 442 Size: 1753 Fragments arranged by size: 1966 1753 958 721 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6373 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2302 2363 2739 5033 1239 Size: 1063 61 376 2294 2579 Fragments arranged by size: 2579 2294 1063 376 61 Bpu10I CC'TnA_GC Cuts at: 3143 3143 Size: 6373 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4498 5363 5661 4498 Size: 865 298 5210 Fragments arranged by size: 5210 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2534 2928 5036 882 Size: 617 1035 394 2108 2219 Fragments arranged by size: 2219 2108 1035 617 394 BsaAI yAC'GTr Cuts at: 493 1204 3042 4164 493 Size: 711 1838 1122 2702 Fragments arranged by size: 2702 1838 1122 711 BsaBI GATnn'nnATC Cuts at: 6367 6367 Size: 6373 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2034 2187 3065 3660 Size: 53 83 186 936 501 153 878 595 Cuts at: 3660 4180 4562 5197 275 Size: 520 382 635 1451 Fragments arranged by size: 1451 936 878 635 595 520 501 382 186 153 83 53 BsaWI w'CCGG_w Cuts at: 2626 4806 5204 5478 5625 6036 2626 Size: 2180 398 274 147 411 2963 Fragments arranged by size: 2963 2180 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3254 3284 1178 Size: 30 165 30 1851 30 4267 Fragments arranged by size: 4267 1851 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3169 3172 1186 Size: 1983 3 4387 Fragments arranged by size: 4387 1983 3 BseYI C'CCAG_C Cuts at: 2208 3587 5576 6072 2208 Size: 1379 1989 496 2509 Fragments arranged by size: 2509 1989 1379 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3038 3631 3689 1163 Size: 573 1302 593 58 3847 Fragments arranged by size: 3847 1302 593 573 58 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3823 4584 5612 1540 Size: 275 186 1822 761 1028 2301 Fragments arranged by size: 2301 1822 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4434 4519 5590 4434 Size: 85 1071 5217 Fragments arranged by size: 5217 1071 85 BsmI GAATG_Cn' Cuts at: 2124 3884 3977 2124 Size: 1760 93 4520 Fragments arranged by size: 4520 1760 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2043 2150 2806 3389 3692 4434 4519 Size: 399 253 107 656 583 303 742 85 Cuts at: 4519 5590 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 656 583 399 303 253 107 85 BspEI T'CCGG_A Cuts at: 6036 6036 Size: 6373 BspHI T'CATG_A Cuts at: 4262 5992 4262 Size: 1730 4643 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3060 3096 3154 3661 3728 844 Size: 2216 36 58 507 67 3489 Fragments arranged by size: 3489 2216 507 67 58 36 BsrBI CCG'CTC Cuts at: 1394 1823 2395 4260 1394 Size: 429 572 1865 3507 Fragments arranged by size: 3507 1865 572 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4868 5042 65 Size: 1183 3620 174 1396 Fragments arranged by size: 3620 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2189 3091 5017 5204 1700 Size: 110 379 902 1926 187 2869 Fragments arranged by size: 2869 1926 902 379 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6373 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6373 BssSI C'ACGA_G Cuts at: 2939 4433 5445 2939 Size: 1494 1012 3867 Fragments arranged by size: 3867 1494 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6373 BstBI TT'CG_AA Cuts at: 5177 5177 Size: 6373 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6373 BstYI r'GATC_y Cuts at: 1972 3466 3618 3703 4070 4455 4472 5924 6010 Size: 1494 152 85 367 385 17 1452 86 Cuts at: 6010 6022 6368 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1494 1452 385 367 346 152 86 85 17 12 Bsu36I CC'TnA_GG Cuts at: 2241 2925 2241 Size: 684 5689 BtgI C'CryG_G Cuts at: 513 1065 2004 2154 2286 513 Size: 552 939 150 132 4600 Fragments arranged by size: 4600 939 552 150 132 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5634 Fragments arranged by size: 5634 683 56 BtsI GCAGTG_nn' Cuts at: 2163 3889 4683 4703 2163 Size: 1726 794 20 3833 Fragments arranged by size: 3833 1726 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6373 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6338 DraI TTT'AAA Cuts at: 3538 3677 3538 Size: 139 6234 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5380 809 Size: 507 4064 1802 Fragments arranged by size: 4064 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3820 4709 Size: 54 1425 50 275 18 177 1813 889 Cuts at: 4709 6338 8 Size: 1629 43 Fragments arranged by size: 1813 1629 1425 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3820 1537 Size: 275 2008 4090 Fragments arranged by size: 4090 2008 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3302 4303 1353 Size: 1949 1001 3423 Fragments arranged by size: 3423 1949 1001 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2205 2767 3351 4960 5334 5480 Size: 1064 138 616 562 584 1609 374 146 Cuts at: 5480 387 Size: 1280 Fragments arranged by size: 1609 1280 1064 616 584 562 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2302 2363 2739 4436 5033 5820 6088 Size: 393 670 61 376 1697 597 787 268 Cuts at: 6088 1239 Size: 1524 Fragments arranged by size: 1697 1524 787 670 597 393 376 268 61 EcoO109I rG'GnC_Cy Cuts at: 2094 2146 2147 2374 2617 2094 Size: 52 1 227 243 5850 Fragments arranged by size: 5850 243 227 52 1 EcoRI G'AATT_C Cuts at: 3682 4060 3682 Size: 378 5995 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2458 2458 Size: 6373 FspI TGC'GCA Cuts at: 4879 4879 Size: 6373 HaeII r_GCGC'y Cuts at: 1678 2190 5520 1678 Size: 512 3330 2531 Fragments arranged by size: 3330 2531 512 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2390 2422 2558 2590 3248 Size: 32 135 32 818 32 136 32 658 Cuts at: 3248 3254 3280 3286 3559 3591 5014 5046 5088 Size: 6 26 6 273 32 1423 32 42 Cuts at: 5088 5120 1373 Size: 32 2626 Fragments arranged by size: 2626 1423 818 658 273 136 135 42 32 32 32 32 32 32 32 26 6 6 HincII GTy'rAC Cuts at: 669 1657 1938 3475 3717 3963 669 Size: 988 281 1537 242 246 3079 Fragments arranged by size: 3079 1537 988 281 246 242 HindIII A'AGCT_T Cuts at: 748 2435 748 Size: 1687 4686 HpaI GTT'AAC Cuts at: 3963 3963 Size: 6373 KasI G'GCGC_C Cuts at: 2186 2186 Size: 6373 KpnI G_GTAC'C Cuts at: 3698 3698 Size: 6373 MfeI C'AATT_G Cuts at: 3972 3972 Size: 6373 MluI A'CGCG_T Cuts at: 6249 6249 Size: 6373 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2874 3081 3228 3300 5487 5671 1305 Size: 1569 207 147 72 2187 184 2007 Fragments arranged by size: 2187 2007 1569 207 184 147 72 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 4374 Fragments arranged by size: 4374 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 3164 3604 4331 4690 4849 518 Size: 648 1998 440 727 359 159 2042 Fragments arranged by size: 2042 1998 727 648 440 359 159 MspA1I CmG'CkG Cuts at: 1606 2026 3430 3591 3665 3789 4466 5614 5859 Size: 420 1404 161 74 124 677 1148 245 Cuts at: 5859 1606 Size: 2120 Fragments arranged by size: 2120 1404 1148 677 420 245 161 124 74 NaeI GCC'GGC Cuts at: 2191 3093 2191 Size: 902 5471 NarI GG'CG_CC Cuts at: 2187 2187 Size: 6373 NcoI C'CATG_G Cuts at: 513 1065 2004 2154 513 Size: 552 939 150 4732 Fragments arranged by size: 4732 939 552 150 NdeI CA'TA_TG Cuts at: 387 387 Size: 6373 NgoMIV G'CCGG_C Cuts at: 2189 3091 2189 Size: 902 5471 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6373 NotI GC'GGCC_GC Cuts at: 3820 3820 Size: 6373 NsiI A_TGCA'T Cuts at: 2816 2816 Size: 6373 NspI r_CATG'y Cuts at: 1132 1229 2207 3163 3731 5276 1132 Size: 97 978 956 568 1545 2229 Fragments arranged by size: 2229 1545 978 956 568 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6373 PciI A'CATG_T Cuts at: 5272 5272 Size: 6373 PflMI CCAn_nnn'nTGG Cuts at: 1108 2830 1108 Size: 1722 4651 PfoI T'CCnGG_A Cuts at: 3621 3621 Size: 6373 PmeI GTTT'AAAC Cuts at: 3677 3677 Size: 6373 PmlI CAC'GTG Cuts at: 3042 3042 Size: 6373 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4442 4474 5955 5987 4442 Size: 32 1481 32 4828 Fragments arranged by size: 4828 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2094 2374 2617 2094 Size: 280 243 5850 Fragments arranged by size: 5850 280 243 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6373 PsiI TTA'TAA Cuts at: 3943 3943 Size: 6373 PspOMI G'GGCC_C Cuts at: 2146 3385 2146 Size: 1239 5134 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3270 3302 3270 Size: 32 6341 PstI C_TGCA'G Cuts at: 830 2170 2202 3725 830 Size: 1340 32 1523 3478 Fragments arranged by size: 3478 1523 1340 32 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6373 PvuII CAG'CTG Cuts at: 2026 3430 2026 Size: 1404 4969 SalI G'TCGA_C Cuts at: 1936 3715 1936 Size: 1779 4594 SbfI CC_TGCA'GG Cuts at: 3725 3725 Size: 6373 ScaI AGT'ACT Cuts at: 2588 4621 6333 2588 Size: 2033 1712 2628 Fragments arranged by size: 2628 2033 1712 SfcI C'TryA_G Cuts at: 826 1045 2166 2198 3721 4856 5537 5728 826 Size: 219 1121 32 1523 1135 681 191 1471 Fragments arranged by size: 1523 1471 1135 1121 681 219 191 32 SfoI GGC'GCC Cuts at: 2188 2188 Size: 6373 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6373 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6373 SmaI CCC'GGG Cuts at: 1872 3700 1872 Size: 1828 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2301 4477 5378 5640 820 Size: 197 926 358 2176 901 262 1553 Fragments arranged by size: 2176 1553 926 901 358 262 197 SnaBI TAC'GTA Cuts at: 493 4164 493 Size: 3671 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6373 SphI G_CATG'C Cuts at: 1132 2207 3163 3731 1132 Size: 1075 956 568 3774 Fragments arranged by size: 3774 1075 956 568 SspI AAT'ATT Cuts at: 5 52 4297 5 Size: 47 4245 2081 Fragments arranged by size: 4245 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6373 StyI C'CwwG_G Cuts at: 513 1065 2004 2154 2266 3344 3500 3812 513 Size: 552 939 150 112 1078 156 312 3074 Fragments arranged by size: 3074 1078 939 552 312 156 150 112 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4400 4417 4570 4755 5200 325 Size: 1255 2820 17 153 185 445 1498 Fragments arranged by size: 2820 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2586 3246 4619 Size: 274 80 33 51 1443 609 660 1373 Cuts at: 4619 6331 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1373 660 609 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6333 6365 6333 Size: 32 6341 Tth111I GACn'n_nGTC Cuts at: 1316 2104 1316 Size: 788 5585 XbaI T'CTAG_A Cuts at: 3709 3709 Size: 6373 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2293 2728 2293 Size: 435 5938 XhoI C'TCGA_G Cuts at: 1943 2301 1943 Size: 358 6015 XmaI C'CCGG_G Cuts at: 1870 3698 1870 Size: 1828 4545 XmnI GAAnn'nnTTC Cuts at: 4502 4502 Size: 6373 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4181 276 Size: 53 83 186 936 2647 2468 Fragments arranged by size: 2647 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI PacI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI