Restriction map for
pFN21AB8104
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# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI AocI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BbeI
BbrPI BbsI BclI BfrI BglII
BlnI BpuAI BsaAI BsaBI BsiWI
BsmBI BsmI BspDI BspHI BsrDI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I ClaI CpoI DraI
DrdI Eam1105I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KasI
KpnI MamI MfeI MluI MslI
MunI NarI NdeI NheI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SbfI ScaI SexAI SfoI SfuI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TfiI Tsp509I Tth111I
XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccIII 509 TCCGGA T^CCGGA
AcyI 796 GGCGTC G[GA]^CG[CT]C
AfaI 864 GTAC GT^AC
AflIII 860 ACATGT A^C[GA][CT]GT
Alw44I 1207 GTGCAC G^TGCAC
ApaLI 1207 GTGCAC G^TGCAC
BamHI 885 GGATCC G^GATCC
BanI 604 GGCACC G^G[CT][GA]CC
BciVI 1156 GTATCC GTATCC(6/5)
BfaI 811 CTAG C^TAG
BlpI 423 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 423 GCTGAGC GC^T[ACGT]AGC
BsaHI 796 GGCGTC G[GA]^CG[CT]C
BsaI 372 GGTCTC GGTCTC(1/5)
BsaWI 509 TCCGGA [AT]^CCGG[AT]
BseAI 509 TCCGGA T^CCGGA
BsmAI 373 GTCTC GTCTC(1/5)
Bsp1407I 863 TGTACA T^GTACA
BspEI 509 TCCGGA T^CCGGA
BspLU11I 860 ACATGT A^CATGT
BsrGI 863 TGTACA T^GTACA
BssSI 1177 CACGAG CACGAG(-5/-1)
BstAPI 744 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 1359 CATCC GGATG(2/0)
CelII 423 GCTGAGC GC^T[ACGT]AGC
EcoNI 810 CCTAGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 1359 CATCC GGATG(9/13)
Hin1I 796 GGCGTC G[GA]^CG[CT]C
HinfI 406 GACTC G^A[ACGT]TC
MaeI 811 CTAG C^TAG
MroI 509 TCCGGA T^CCGGA
MseI 593 TTAA T^TAA
NaeI 1228 GCCGGC GCC^GGC
NcoI 515 CCATGG C^CATGG
NgoMIV 1228 GCCGGC G^CCGGC
NotI 139 GCGGCCGC GC^GGCCGC
PaeR7I 400 CTCGAG C^TCGAG
PleI 406 GACTC GAGTC(4/5)
RsaI 864 GTAC GT^AC
SfiI 165 GGCCCGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 400 CTCGAG C^T[CT][GA]AG
SspBI 863 TGTACA T^GTACA
Tru9I 593 TTAA T^TAA
XhoI 400 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwNI 745 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
1279 CAGATCCTG
BalI 1275 TGGCCA TGG^CCA
1364 TGGCCA
BbvCI 238 CCTCAGC CCTCAGC(-5/-2)
1107 GCTGAGG
BcgI 974 GCACCACCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1059 GCAGGTGCTTCG
BsgI 457 GTGCAG GTGCAG(16/14)
565 GTGCAG
BssHII 118 GCGCGC G^CGCGC
921 GCGCGC
DraIII 703 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
1177 CACGAGGTG
EagI 34 CGGCCG C^GGCCG
140 CGGCCG
EclXI 34 CGGCCG C^GGCCG
140 CGGCCG
Eco52I 34 CGGCCG C^GGCCG
140 CGGCCG
EcoT14I 515 CCATGG C^C[AT][AT]GG
827 CCAAGG
MaeIII 250 GTGAC ^GT[ACGT]AC
928 GTCAC
MluNI 1275 TGGCCA TGG^CCA
1364 TGGCCA
MscI 1275 TGGCCA TGG^CCA
1364 TGGCCA
NspI 860 ACATGT [GA]CATG^[CT]
944 ACATGC
PflMI 702 CCACGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1278 CCAGATCCTGG
PpuMI 194 AGGTCCC [GA]G^G[AT]CC[CT]
746 AGGACCT
PshAI 478 GACGTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
513 GACCATGGTC
SapI 108 GCTCTTC GCTCTTC(1/4)
329 GCTCTTC
SgrAI 1227 CGCCGGCG C[GA]^CCGG[CT]G
1291 CACCGGCG
StyI 515 CCATGG C^C[AT][AT]GG
827 CCAAGG
Tsp45I 250 GTGAC ^GT[GC]AC
928 GTCAC
Van91I 702 CCACGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1278 CCAGATCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AluI AlwI Aor51HI
ApaI AspHI AvaI AvaII BanII
BbvI BcnI BglI BmyI BpmI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco47III EcoO109I EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI HinP1I HpaII HphI
ItaI Ksp632I KspI MaeII MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PstI PvuII SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI TaqI TseI TspRI TthHB8I
XhoII XmaCI XmaI