(Circular) (Six-base) MAPSORT of: pFN21AB8104.seq Check: 8044 from: 1 to: 6088 pFN21AB8104 6088 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2352 3055 2352 Size: 703 5385 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3898 278 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3431 1937 Size: 1494 4594 Acc65I G'GTAC_C Cuts at: 3409 3409 Size: 6088 AclI AA'CG_TT Cuts at: 1563 4215 4588 1563 Size: 2652 373 3063 Fragments arranged by size: 3063 2652 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2648 2916 3157 4151 5535 5803 1632 Size: 1016 268 241 994 1384 268 1917 Fragments arranged by size: 1917 1384 1016 994 268 268 241 AfeI AGC'GCT Cuts at: 2558 2633 2651 2558 Size: 75 18 5995 Fragments arranged by size: 5995 75 18 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5891 AflIII A'CryG_T Cuts at: 1203 2865 4987 5964 1203 Size: 1662 2122 977 1327 Fragments arranged by size: 2122 1662 1327 977 AgeI A'CCGG_T Cuts at: 4919 4919 Size: 6088 AhdI GACnn_n'nnGTC Cuts at: 4817 4817 Size: 6088 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6088 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6088 AlwNI CAG_nnn'CTG Cuts at: 2755 3289 3831 5403 2755 Size: 534 542 1572 3440 Fragments arranged by size: 3440 1572 542 534 ApaI G_GGCC'C Cuts at: 2024 2083 2173 2024 Size: 59 90 5939 Fragments arranged by size: 5939 90 59 ApaLI G'TGCA_C Cuts at: 3212 4145 5301 3212 Size: 933 1156 3999 Fragments arranged by size: 3999 1156 933 ApoI r'AATT_y Cuts at: 1493 3397 3623 3775 5884 1493 Size: 1904 226 152 2109 1697 Fragments arranged by size: 2109 1904 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4642 160 Size: 872 3610 1606 Fragments arranged by size: 3610 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 AvaI C'yCGr_G Cuts at: 1870 1943 2015 2065 2166 2405 3413 1870 Size: 73 72 50 101 239 1008 4545 Fragments arranged by size: 4545 1008 239 101 73 72 50 BamHI G'GATC_C Cuts at: 2890 3418 3785 2890 Size: 528 367 5193 Fragments arranged by size: 5193 528 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2609 3409 4864 618 Size: 325 217 627 822 800 1455 1842 Fragments arranged by size: 1842 1455 822 800 627 325 217 BanII G_rGCy'C Cuts at: 1391 2024 2083 2166 2173 2224 2271 2336 3407 Size: 633 59 83 7 51 47 65 1071 Cuts at: 3407 1391 Size: 4072 Fragments arranged by size: 4072 1071 633 83 65 59 51 47 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5117 BbvCI CC'TCA_GC Cuts at: 2244 3113 2244 Size: 869 5219 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2968 3002 3053 3087 4279 4313 1903 Size: 34 1031 34 51 34 1192 34 3678 Fragments arranged by size: 3678 1192 1031 51 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3172 3980 5190 3172 Size: 808 1210 4070 Fragments arranged by size: 4070 1210 808 BclI T'GATC_A Cuts at: 1548 1548 Size: 6088 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2028 2177 3377 Size: 107 122 71 631 605 356 149 1200 Cuts at: 3377 4699 136 Size: 1322 1525 Fragments arranged by size: 1525 1322 1200 631 605 356 149 122 107 71 BglII A'GATC_T Cuts at: 6083 6083 Size: 6088 BlpI GC'TnA_GC Cuts at: 2429 3505 2429 Size: 1076 5012 Bme1580I G_kGCm'C Cuts at: 1790 2024 2083 2173 2280 2586 2612 3216 4149 Size: 234 59 90 107 306 26 604 933 Cuts at: 4149 5305 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 933 604 306 234 107 90 59 26 BmgBI CAC'GTC Cuts at: 1318 2275 2485 1318 Size: 957 210 4921 Fragments arranged by size: 4921 957 210 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2598 3819 4777 Size: 416 53 83 399 24 1181 1221 958 Cuts at: 4777 442 Size: 1753 Fragments arranged by size: 1753 1221 1181 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6088 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2093 2959 3176 4748 1239 Size: 854 866 217 1572 2579 Fragments arranged by size: 2579 1572 866 854 217 Bpu10I CC'TnA_GC Cuts at: 2244 3113 2244 Size: 869 5219 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4213 5078 5376 4213 Size: 865 298 4925 Fragments arranged by size: 4925 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2383 4751 882 Size: 617 884 2368 2219 Fragments arranged by size: 2368 2219 884 617 BsaAI yAC'GTr Cuts at: 493 1204 3879 493 Size: 711 2675 2702 Fragments arranged by size: 2702 2675 711 BsaBI GATnn'nnATC Cuts at: 6082 6082 Size: 6088 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2802 3895 4277 4912 Size: 53 83 186 936 1269 1093 382 635 Cuts at: 4912 275 Size: 1451 Fragments arranged by size: 1451 1269 1093 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2514 4521 4919 5193 5340 5751 2514 Size: 2007 398 274 147 411 2851 Fragments arranged by size: 2851 2007 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5863 Fragments arranged by size: 5863 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2279 2371 2383 2834 2837 2924 2927 3194 Size: 1093 92 12 451 3 87 3 267 Cuts at: 3194 3326 1186 Size: 132 3948 Fragments arranged by size: 3948 1093 451 267 132 92 87 12 3 3 BseYI C'CCAG_C Cuts at: 3340 5291 5787 3340 Size: 1951 496 3641 Fragments arranged by size: 3641 1951 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2483 2591 1163 Size: 573 747 108 4660 Fragments arranged by size: 4660 747 573 108 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2042 2139 2148 3538 4299 5327 Size: 275 186 41 97 9 1390 761 1028 Cuts at: 5327 1540 Size: 2301 Fragments arranged by size: 2301 1390 1028 761 275 186 97 41 9 BsiHKAI G_wGCw'C Cuts at: 2787 2919 3216 4149 4234 5305 2787 Size: 132 297 933 85 1071 3570 Fragments arranged by size: 3570 1071 933 297 132 85 BsmI GAATG_Cn' Cuts at: 3599 3692 3599 Size: 93 5995 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2083 2166 2173 2224 2271 2280 Size: 399 234 59 83 7 51 47 9 Cuts at: 2280 2336 2586 2612 2787 2919 3216 3407 4149 Size: 56 250 26 175 132 297 191 742 Cuts at: 4149 4234 5305 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 297 250 234 191 175 132 85 83 59 56 51 47 26 9 7 BspEI T'CCGG_A Cuts at: 2514 5751 2514 Size: 3237 2851 BspHI T'CATG_A Cuts at: 3977 5707 3977 Size: 1730 4358 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2352 2433 2748 2763 3055 3443 844 Size: 1508 81 315 15 292 388 3489 Fragments arranged by size: 3489 1508 388 315 292 81 15 BsrBI CCG'CTC Cuts at: 1394 1823 2077 2305 2845 3975 1394 Size: 429 254 228 540 1130 3507 Fragments arranged by size: 3507 1130 540 429 254 228 BsrDI GCAATG_nn' Cuts at: 65 1248 4583 4757 65 Size: 1183 3335 174 1396 Fragments arranged by size: 3335 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2533 3233 3297 4732 4919 1700 Size: 110 723 700 64 1435 187 2869 Fragments arranged by size: 2869 1435 723 700 187 110 64 BsrGI T'GTAC_A Cuts at: 96 2868 96 Size: 2772 3316 BssHII G'CGCG_C Cuts at: 1925 2123 2926 1925 Size: 198 803 5087 Fragments arranged by size: 5087 803 198 BssSI C'ACGA_G Cuts at: 3182 4148 5160 3182 Size: 966 1012 4110 Fragments arranged by size: 4110 1012 966 BstAPI GCAn_nnn'nTGC Cuts at: 794 2755 794 Size: 1961 4127 BstBI TT'CG_AA Cuts at: 4892 4892 Size: 6088 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6088 BstYI r'GATC_y Cuts at: 1972 2511 2890 3141 3285 3418 3785 4170 4187 Size: 539 379 251 144 133 367 385 17 Cuts at: 4187 5639 5725 5737 6083 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 539 385 379 367 346 251 144 133 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2154 2173 2216 2452 2520 2707 2904 Size: 552 1089 19 43 236 68 187 197 Cuts at: 2904 513 Size: 3697 Fragments arranged by size: 3697 1089 552 236 197 187 68 43 19 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3212 487 Size: 683 56 1986 3363 Fragments arranged by size: 3363 1986 683 56 BtsI GCAGTG_nn' Cuts at: 2960 3220 3604 4398 4418 2960 Size: 260 384 794 20 4630 Fragments arranged by size: 4630 794 384 260 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6088 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2170 2205 529 Size: 35 1606 35 4412 Fragments arranged by size: 4412 1606 35 35 DraI TTT'AAA Cuts at: 3392 3392 Size: 6088 DraIII CAC_nnn'GTG Cuts at: 2713 3187 2713 Size: 474 5614 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5095 809 Size: 507 3779 1802 Fragments arranged by size: 3779 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2039 2145 3280 Size: 54 1425 50 275 18 209 106 1135 Cuts at: 3280 3369 3535 4424 6053 8 Size: 89 166 889 1629 43 Fragments arranged by size: 1629 1425 1135 889 275 209 166 106 89 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2039 2145 3535 1537 Size: 275 227 106 1390 4090 Fragments arranged by size: 4090 1390 275 227 106 EarI CTCTTCn'nnn_ Cuts at: 1353 2120 2341 3078 4018 1353 Size: 767 221 737 940 3423 Fragments arranged by size: 3423 940 767 737 221 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4675 5049 5195 387 Size: 1064 138 3086 374 146 1280 Fragments arranged by size: 3086 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2093 2648 2916 2959 3157 3176 4151 Size: 393 461 555 268 43 198 19 975 Cuts at: 4151 4748 5535 5803 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 975 787 597 555 461 393 268 268 198 43 19 EcoNI CCTnn'n_nnAGG Cuts at: 2819 2819 Size: 6088 EcoO109I rG'GnC_Cy Cuts at: 2020 2055 2080 2200 2752 2020 Size: 35 25 120 552 5356 Fragments arranged by size: 5356 552 120 35 25 EcoRI G'AATT_C Cuts at: 3397 3775 3397 Size: 378 5710 FspI TGC'GCA Cuts at: 4594 4594 Size: 6088 HaeII r_GCGC'y Cuts at: 1678 2560 2635 2653 5235 1678 Size: 882 75 18 2582 2531 Fragments arranged by size: 2582 2531 882 75 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3106 3138 4729 4761 4803 Size: 32 135 32 1534 32 1591 32 42 Cuts at: 4803 4835 1373 Size: 32 2626 Fragments arranged by size: 2626 1591 1534 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3432 3678 669 Size: 988 281 1494 246 3079 Fragments arranged by size: 3079 1494 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6088 HpaI GTT'AAC Cuts at: 3678 3678 Size: 6088 KpnI G_GTAC'C Cuts at: 3413 3413 Size: 6088 MfeI C'AATT_G Cuts at: 3687 3687 Size: 6088 MluI A'CGCG_T Cuts at: 5964 5964 Size: 6088 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5202 5386 1305 Size: 3897 184 2007 Fragments arranged by size: 3897 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3282 3371 10 Size: 54 1425 343 1450 89 2727 Fragments arranged by size: 2727 1450 1425 343 89 54 MslI CAynn'nnrTG Cuts at: 518 1166 4046 4405 4564 518 Size: 648 2880 359 159 2042 Fragments arranged by size: 2880 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2156 2175 2218 2248 2330 2454 2467 2575 Size: 550 19 43 30 82 124 13 108 Cuts at: 2575 2616 2746 3504 4181 5329 5574 1606 Size: 41 130 758 677 1148 245 2120 Fragments arranged by size: 2120 1148 758 677 550 245 130 124 108 82 43 41 30 19 13 NaeI GCC'GGC Cuts at: 3235 3235 Size: 6088 NcoI C'CATG_G Cuts at: 513 1065 2520 513 Size: 552 1455 4081 Fragments arranged by size: 4081 1455 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6088 NgoMIV G'CCGG_C Cuts at: 3233 3233 Size: 6088 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6088 NotI GC'GGCC_GC Cuts at: 2145 3535 2145 Size: 1390 4698 NspI r_CATG'y Cuts at: 1132 1229 2869 2953 3446 4991 1132 Size: 97 1640 84 493 1545 2229 Fragments arranged by size: 2229 1640 1545 493 97 84 PasI CC'CwG_GG Cuts at: 1348 2291 3306 1348 Size: 943 1015 4130 Fragments arranged by size: 4130 1015 943 PciI A'CATG_T Cuts at: 2865 4987 2865 Size: 2122 3966 PflMI CCAn_nnn'nTGG Cuts at: 1108 2713 3289 1108 Size: 1605 576 3907 Fragments arranged by size: 3907 1605 576 PfoI T'CCnGG_A Cuts at: 3144 3288 3144 Size: 144 5944 PmeI GTTT'AAAC Cuts at: 3392 3392 Size: 6088 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4157 4189 5670 5702 4157 Size: 32 1481 32 4543 Fragments arranged by size: 4543 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2200 2752 2200 Size: 552 5536 PshAI GACnn'nnGTC Cuts at: 1613 2487 2522 1613 Size: 874 35 5179 Fragments arranged by size: 5179 874 35 PsiI TTA'TAA Cuts at: 3658 3658 Size: 6088 PspOMI G'GGCC_C Cuts at: 2020 2079 2169 2020 Size: 59 90 5939 Fragments arranged by size: 5939 90 59 PspXI vC'TCGA_Gb Cuts at: 2405 2405 Size: 6088 PstI C_TGCA'G Cuts at: 830 2063 2349 2745 2751 3032 3138 3440 830 Size: 1233 286 396 6 281 106 302 3478 Fragments arranged by size: 3478 1233 396 302 286 281 106 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6088 PvuII CAG'CTG Cuts at: 2248 2467 2575 2746 2248 Size: 219 108 171 5590 Fragments arranged by size: 5590 219 171 108 SacII CC_GC'GG Cuts at: 2157 2176 2219 2455 2157 Size: 19 43 236 5790 Fragments arranged by size: 5790 236 43 19 SalI G'TCGA_C Cuts at: 1936 3430 1936 Size: 1494 4594 SapI GCTCTTCn'nnn_ Cuts at: 2120 2341 2120 Size: 221 5867 SbfI CC_TGCA'GG Cuts at: 3440 3440 Size: 6088 ScaI AGT'ACT Cuts at: 4336 6048 4336 Size: 1712 4376 SfcI C'TryA_G Cuts at: 826 1045 2059 2345 2741 2747 3028 3134 3436 Size: 219 1014 286 396 6 281 106 302 Cuts at: 3436 4571 5252 5443 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1014 681 396 302 286 281 219 191 106 6 SfiI GGCCn_nnn'nGGCC Cuts at: 2177 2177 Size: 6088 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 SgrAI Cr'CCGG_yG Cuts at: 1700 3233 3297 1700 Size: 1533 64 4491 Fragments arranged by size: 4491 1533 64 SmaI CCC'GGG Cuts at: 1872 2017 2067 2168 3415 1872 Size: 145 50 101 1247 4545 Fragments arranged by size: 4545 1247 145 101 50 SmlI C'TyrA_G Cuts at: 820 1017 1943 2405 4192 5093 5355 820 Size: 197 926 462 1787 901 262 1553 Fragments arranged by size: 1787 1553 926 901 462 262 197 SnaBI TAC'GTA Cuts at: 493 3879 493 Size: 3386 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6088 SphI G_CATG'C Cuts at: 1132 3446 1132 Size: 2314 3774 SspI AAT'ATT Cuts at: 5 52 4012 5 Size: 47 3960 2081 Fragments arranged by size: 3960 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6088 StyI C'CwwG_G Cuts at: 513 1065 2520 2832 3527 513 Size: 552 1455 312 695 3074 Fragments arranged by size: 3074 1455 695 552 312 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2365 2557 4115 4132 4285 4470 4915 Size: 1255 785 192 1558 17 153 185 445 Cuts at: 4915 325 Size: 1498 Fragments arranged by size: 1558 1498 1255 785 445 192 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2868 4334 6046 Size: 274 80 33 51 1443 891 1466 1712 Cuts at: 6046 96 Size: 138 Fragments arranged by size: 1712 1466 1443 891 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2766 2798 6048 6080 2766 Size: 32 3250 32 2774 Fragments arranged by size: 3250 2774 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6088 XbaI T'CTAG_A Cuts at: 3424 3424 Size: 6088 XhoI C'TCGA_G Cuts at: 1943 2405 1943 Size: 462 5626 XmaI C'CCGG_G Cuts at: 1870 2015 2065 2166 3413 1870 Size: 145 50 101 1247 4545 Fragments arranged by size: 4545 1247 145 101 50 XmnI GAAnn'nnTTC Cuts at: 4217 4217 Size: 6088 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3896 276 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PsrI RsrII SacI SanDI SexAI SfoI SrfI SwaI XcmI