Restriction map for
pFN21AB8104
(Linear) (Six-base) MAPSORT of: KIBB8104.seq Check: 9183 from: 1 to: 1383
KIBB8104.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 347 1050 1383
Size: 347 703 333
Fragments arranged by size:
703 347 333
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 643 911 1152 1383
Size: 643 268 241 231
Fragments arranged by size:
643 268 241 231
AfeI AGC'GCT
Cuts at: 0 553 628 646 1383
Size: 553 75 18 737
Fragments arranged by size:
737 553 75 18
AflIII A'CryG_T
Cuts at: 0 860 1383
Size: 860 523
AlwNI CAG_nnn'CTG
Cuts at: 0 750 1284 1383
Size: 750 534 99
Fragments arranged by size:
750 534 99
ApaI G_GGCC'C
Cuts at: 0 19 78 168 1383
Size: 19 59 90 1215
Fragments arranged by size:
1215 90 59 19
ApaLI G'TGCA_C
Cuts at: 0 1207 1383
Size: 1207 176
AvaI C'yCGr_G
Cuts at: 0 10 60 161 400 1383
Size: 10 50 101 239 983
Fragments arranged by size:
983 239 101 50 10
BamHI G'GATC_C
Cuts at: 0 885 1383
Size: 885 498
BanI G'GyrC_C
Cuts at: 0 604 1383
Size: 604 779
BanII G_rGCy'C
Cuts at: 0 19 78 161 168 219 266 331 1383
Size: 19 59 83 7 51 47 65 1052
Fragments arranged by size:
1052 83 65 59 51 47 19 7
BbvCI CC'TCA_GC
Cuts at: 0 239 1108 1383
Size: 239 869 275
Fragments arranged by size:
869 275 239
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 963 997 1048 1082 1383
Size: 963 34 51 34 301
Fragments arranged by size:
963 301 51 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1167 1383
Size: 1167 216
BglI GCCn_nnn'nGGC
Cuts at: 0 23 172 1372 1383
Size: 23 149 1200 11
Fragments arranged by size:
1200 149 23 11
BlpI GC'TnA_GC
Cuts at: 0 424 1383
Size: 424 959
Bme1580I G_kGCm'C
Cuts at: 0 19 78 168 275 581 607 1211 1383
Size: 19 59 90 107 306 26 604 172
Fragments arranged by size:
604 306 172 107 90 59 26 19
BmgBI CAC'GTC
Cuts at: 0 270 480 1383
Size: 270 210 903
Fragments arranged by size:
903 270 210
BmrI ACTGGGnnnn_n'
Cuts at: 0 593 1383
Size: 593 790
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 954 1171 1383
Size: 88 866 217 212
Fragments arranged by size:
866 217 212 88
Bpu10I CC'TnA_GC
Cuts at: 0 239 1108 1383
Size: 239 869 275
Fragments arranged by size:
869 275 239
BsaI GGTCTCn'nnnn_
Cuts at: 0 378 1383
Size: 378 1005
BsaHI Gr'CG_yC
Cuts at: 0 797 1383
Size: 797 586
BsaWI w'CCGG_w
Cuts at: 0 509 1383
Size: 509 874
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 274 366 378 829 832 919 922 1189
Size: 274 92 12 451 3 87 3 267
Cuts at: 1189 1321 1383
Size: 132 62
Fragments arranged by size:
451 274 267 132 92 87 62 12
3 3
BseYI C'CCAG_C
Cuts at: 0 1335 1383
Size: 1335 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 586 1383
Size: 478 108 797
Fragments arranged by size:
797 478 108
BsiEI CG_ry'CG
Cuts at: 0 37 134 143 1383
Size: 37 97 9 1240
Fragments arranged by size:
1240 97 37 9
BsiHKAI G_wGCw'C
Cuts at: 0 782 914 1211 1383
Size: 782 132 297 172
Fragments arranged by size:
782 297 172 132
Bsp1286I G_dGCh'C
Cuts at: 0 19 78 161 168 219 266 275 331
Size: 19 59 83 7 51 47 9 56
Cuts at: 331 581 607 782 914 1211 1383
Size: 250 26 175 132 297 172
Fragments arranged by size:
297 250 175 172 132 83 59 56
51 47 26 19 9 7
BspEI T'CCGG_A
Cuts at: 0 509 1383
Size: 509 874
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 347 428 743 758 1050 1383
Size: 347 81 315 15 292 333
Fragments arranged by size:
347 333 315 292 81 15
BsrBI CCG'CTC
Cuts at: 0 72 300 840 1383
Size: 72 228 540 543
Fragments arranged by size:
543 540 228 72
BsrFI r'CCGG_y
Cuts at: 0 528 1228 1292 1383
Size: 528 700 64 91
Fragments arranged by size:
700 528 91 64
BsrGI T'GTAC_A
Cuts at: 0 863 1383
Size: 863 520
BssHII G'CGCG_C
Cuts at: 0 118 921 1383
Size: 118 803 462
Fragments arranged by size:
803 462 118
BssSI C'ACGA_G
Cuts at: 0 1177 1383
Size: 1177 206
BstAPI GCAn_nnn'nTGC
Cuts at: 0 750 1383
Size: 750 633
BstYI r'GATC_y
Cuts at: 0 506 885 1136 1280 1383
Size: 506 379 251 144 103
Fragments arranged by size:
506 379 251 144 103
BtgI C'CryG_G
Cuts at: 0 149 168 211 447 515 702 899 1383
Size: 149 19 43 236 68 187 197 484
Fragments arranged by size:
484 236 197 187 149 68 43 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1207 1383
Size: 1207 176
BtsI GCAGTG_nn'
Cuts at: 0 955 1215 1383
Size: 955 260 168
Fragments arranged by size:
955 260 168
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 165 200 1383
Size: 165 35 1183
Fragments arranged by size:
1183 165 35
DraIII CAC_nnn'GTG
Cuts at: 0 708 1182 1383
Size: 708 474 201
Fragments arranged by size:
708 474 201
EaeI y'GGCC_r
Cuts at: 0 34 140 1275 1364 1383
Size: 34 106 1135 89 19
Fragments arranged by size:
1135 106 89 34 19
EagI C'GGCC_G
Cuts at: 0 34 140 1383
Size: 34 106 1243
Fragments arranged by size:
1243 106 34
EarI CTCTTCn'nnn_
Cuts at: 0 115 336 1073 1383
Size: 115 221 737 310
Fragments arranged by size:
737 310 221 115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 643 911 954 1152 1171 1383
Size: 88 555 268 43 198 19 212
Fragments arranged by size:
555 268 212 198 88 43 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 814 1383
Size: 814 569
EcoO109I rG'GnC_Cy
Cuts at: 0 15 50 75 195 747 1383
Size: 15 35 25 120 552 636
Fragments arranged by size:
636 552 120 35 25 15
HaeII r_GCGC'y
Cuts at: 0 555 630 648 1383
Size: 555 75 18 735
Fragments arranged by size:
735 555 75 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1101 1133 1383
Size: 1101 32 250
Fragments arranged by size:
1101 250 32
MscI TGG'CCA
Cuts at: 0 1277 1366 1383
Size: 1277 89 17
Fragments arranged by size:
1277 89 17
MspA1I CmG'CkG
Cuts at: 0 151 170 213 243 325 449 462 570
Size: 151 19 43 30 82 124 13 108
Cuts at: 570 611 741 1383
Size: 41 130 642
Fragments arranged by size:
642 151 130 124 108 82 43 41
30 19 13
NaeI GCC'GGC
Cuts at: 0 1230 1383
Size: 1230 153
NcoI C'CATG_G
Cuts at: 0 515 1383
Size: 515 868
NgoMIV G'CCGG_C
Cuts at: 0 1228 1383
Size: 1228 155
NotI GC'GGCC_GC
Cuts at: 0 140 1383
Size: 140 1243
NspI r_CATG'y
Cuts at: 0 864 948 1383
Size: 864 84 435
Fragments arranged by size:
864 435 84
PasI CC'CwG_GG
Cuts at: 0 286 1301 1383
Size: 286 1015 82
Fragments arranged by size:
1015 286 82
PciI A'CATG_T
Cuts at: 0 860 1383
Size: 860 523
PflMI CCAn_nnn'nTGG
Cuts at: 0 708 1284 1383
Size: 708 576 99
Fragments arranged by size:
708 576 99
PfoI T'CCnGG_A
Cuts at: 0 1139 1283 1383
Size: 1139 144 100
Fragments arranged by size:
1139 144 100
PpuMI rG'GwC_Cy
Cuts at: 0 195 747 1383
Size: 195 552 636
Fragments arranged by size:
636 552 195
PshAI GACnn'nnGTC
Cuts at: 0 482 517 1383
Size: 482 35 866
Fragments arranged by size:
866 482 35
PspOMI G'GGCC_C
Cuts at: 0 15 74 164 1383
Size: 15 59 90 1219
Fragments arranged by size:
1219 90 59 15
PspXI vC'TCGA_Gb
Cuts at: 0 400 1383
Size: 400 983
PstI C_TGCA'G
Cuts at: 0 58 344 740 746 1027 1133 1383
Size: 58 286 396 6 281 106 250
Fragments arranged by size:
396 286 281 250 106 58 6
PvuII CAG'CTG
Cuts at: 0 243 462 570 741 1383
Size: 243 219 108 171 642
Fragments arranged by size:
642 243 219 171 108
SacII CC_GC'GG
Cuts at: 0 152 171 214 450 1383
Size: 152 19 43 236 933
Fragments arranged by size:
933 236 152 43 19
SapI GCTCTTCn'nnn_
Cuts at: 0 115 336 1383
Size: 115 221 1047
Fragments arranged by size:
1047 221 115
SfcI C'TryA_G
Cuts at: 0 54 340 736 742 1023 1129 1383
Size: 54 286 396 6 281 106 254
Fragments arranged by size:
396 286 281 254 106 54 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 172 1383
Size: 172 1211
SgrAI Cr'CCGG_yG
Cuts at: 0 1228 1292 1383
Size: 1228 64 91
Fragments arranged by size:
1228 91 64
SmaI CCC'GGG
Cuts at: 0 12 62 163 1383
Size: 12 50 101 1220
Fragments arranged by size:
1220 101 50 12
SmlI C'TyrA_G
Cuts at: 0 400 1383
Size: 400 983
StyI C'CwwG_G
Cuts at: 0 515 827 1383
Size: 515 312 556
Fragments arranged by size:
556 515 312
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 360 552 1383
Size: 360 192 831
Fragments arranged by size:
831 360 192
TatI w'GTAC_w
Cuts at: 0 863 1383
Size: 863 520
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 761 793 1383
Size: 761 32 590
Fragments arranged by size:
761 590 32
XhoI C'TCGA_G
Cuts at: 0 400 1383
Size: 400 983
XmaI C'CCGG_G
Cuts at: 0 10 60 161 1383
Size: 10 50 101 1222
Fragments arranged by size:
1222 101 50 10
Enzymes that do cut:
AarI AcuI AfeI AflIII AlwNI ApaI ApaLI AvaI
BamHI BanI BanII BbvCI BcgI BciVI BglI BlpI
Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstYI BtgI
BtgZI BtsI CspCI DraIII EaeI EagI EarI Eco57MI
EcoNI EcoO109I HaeII Hin4I MscI MspA1I NaeI NcoI
NgoMIV NotI NspI PasI PciI PflMI PfoI PpuMI
PshAI PspOMI PspXI PstI PvuII SacII SapI SfcI
SfiI SgrAI SmaI SmlI StyI TaqII TatI TstI
XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BbeI BbsI BclI BfrBI BglII BmtI BplI BpuEI
BsaAI BsaBI BsaXI BsiWI BsmI BsmBI BspHI BsrDI
BstBI BstEII BstXI BstZ17I Bsu36I ClaI DraI DrdI
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MslI NarI NdeI NheI NruI NsiI PacI PmeI
PmlI PpiI PsiI PsrI PvuI RsrII SacI SalI
SanDI SbfI ScaI SexAI SfoI SgfI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI
XmnI ZraI