(Circular) (Six-base) MAPSORT of: pFN21AB7747.seq Check: 2668 from: 1 to: 6031 pFN21AB7747 6031 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3841 278 Size: 53 83 186 936 2305 2468 Fragments arranged by size: 2468 2305 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3374 1937 Size: 1437 4594 Acc65I G'GTAC_C Cuts at: 3352 3352 Size: 6031 AclI AA'CG_TT Cuts at: 1563 4158 4531 1563 Size: 2595 373 3063 Fragments arranged by size: 3063 2595 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2360 4094 5478 5746 1632 Size: 728 1734 1384 268 1917 Fragments arranged by size: 1917 1734 1384 728 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5834 AflIII A'CryG_T Cuts at: 1203 3226 3240 4930 5907 1203 Size: 2023 14 1690 977 1327 Fragments arranged by size: 2023 1690 1327 977 14 AgeI A'CCGG_T Cuts at: 4862 4862 Size: 6031 AhdI GACnn_n'nnGTC Cuts at: 3121 4760 3121 Size: 1639 4392 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6031 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6031 AlwNI CAG_nnn'CTG Cuts at: 2276 2911 3774 5346 2276 Size: 635 863 1572 2961 Fragments arranged by size: 2961 1572 863 635 ApaLI G'TGCA_C Cuts at: 4088 5244 4088 Size: 1156 4875 ApoI r'AATT_y Cuts at: 1493 2488 3340 3566 3718 5827 1493 Size: 995 852 226 152 2109 1697 Fragments arranged by size: 2109 1697 995 852 226 152 AseI AT'TA_AT Cuts at: 160 1032 4585 160 Size: 872 3553 1606 Fragments arranged by size: 3553 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6031 AvaI C'yCGr_G Cuts at: 1870 1943 3356 1870 Size: 73 1413 4545 Fragments arranged by size: 4545 1413 73 AvrII C'CTAG_G Cuts at: 2957 2957 Size: 6031 BamHI G'GATC_C Cuts at: 3361 3728 3361 Size: 367 5664 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3352 4807 618 Size: 325 217 627 1565 1455 1842 Fragments arranged by size: 1842 1565 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3350 1391 Size: 1959 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2041 928 Size: 971 142 4918 Fragments arranged by size: 4918 971 142 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2282 2316 4222 4256 1903 Size: 34 345 34 1906 34 3678 Fragments arranged by size: 3678 1906 345 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2347 3923 5133 2347 Size: 1576 1210 3245 Fragments arranged by size: 3245 1576 1210 BclI T'GATC_A Cuts at: 1548 2973 1548 Size: 1425 4606 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4642 136 Size: 107 122 71 631 605 337 2633 1525 Fragments arranged by size: 2633 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6026 6026 Size: 6031 BlpI GC'TnA_GC Cuts at: 2082 3448 2082 Size: 1366 4665 Bme1580I G_kGCm'C Cuts at: 1790 4092 5248 1790 Size: 2302 1156 2573 Fragments arranged by size: 2573 2302 1156 BmgBI CAC'GTC Cuts at: 1318 3227 3241 1318 Size: 1909 14 4108 Fragments arranged by size: 4108 1909 14 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2440 3762 4720 Size: 416 53 83 399 24 1023 1322 958 Cuts at: 4720 442 Size: 1753 Fragments arranged by size: 1753 1322 1023 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6031 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2588 4691 1239 Size: 1349 2103 2579 Fragments arranged by size: 2579 2103 1349 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2129 2156 2580 3031 4156 5021 5319 2129 Size: 27 424 451 1125 865 298 2841 Fragments arranged by size: 2841 1125 865 451 424 298 27 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2657 4694 882 Size: 617 1158 2037 2219 Fragments arranged by size: 2219 2037 1158 617 BsaAI yAC'GTr Cuts at: 493 1204 3822 493 Size: 711 2618 2702 Fragments arranged by size: 2702 2618 711 BsaBI GATnn'nnATC Cuts at: 6025 6025 Size: 6031 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3161 3838 4220 4855 Size: 53 83 186 936 1628 677 382 635 Cuts at: 4855 275 Size: 1451 Fragments arranged by size: 1628 1451 936 677 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2510 4464 4862 5136 5283 5694 2510 Size: 1954 398 274 147 411 2847 Fragments arranged by size: 2847 1954 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2741 2771 1178 Size: 30 165 30 1338 30 4438 Fragments arranged by size: 4438 1338 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2308 2441 1186 Size: 1122 133 4776 Fragments arranged by size: 4776 1122 133 BseYI C'CCAG_C Cuts at: 2033 5234 5730 2033 Size: 3201 496 2334 Fragments arranged by size: 3201 2334 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2171 3071 3294 1163 Size: 573 435 900 223 3900 Fragments arranged by size: 3900 900 573 435 223 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3481 4242 5270 1540 Size: 275 186 1480 761 1028 2301 Fragments arranged by size: 2301 1480 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4092 4177 5248 4092 Size: 85 1071 4875 Fragments arranged by size: 4875 1071 85 BsmI GAATG_Cn' Cuts at: 3542 3635 3542 Size: 93 5938 BsmBI CGTCTCn'nnnn_ Cuts at: 2390 2390 Size: 6031 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3350 4092 4177 5248 1391 Size: 399 1560 742 85 1071 2174 Fragments arranged by size: 2174 1560 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5694 5694 Size: 6031 BspHI T'CATG_A Cuts at: 3920 5650 3920 Size: 1730 4301 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3386 844 Size: 2542 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2891 3918 1394 Size: 429 1068 1027 3507 Fragments arranged by size: 3507 1068 1027 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2248 4526 4700 65 Size: 1183 1000 2278 174 1396 Fragments arranged by size: 2278 1396 1183 1000 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2022 4675 4862 1700 Size: 110 212 2653 187 2869 Fragments arranged by size: 2869 2653 212 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6031 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6031 BssSI C'ACGA_G Cuts at: 2781 4091 5103 2781 Size: 1310 1012 3709 Fragments arranged by size: 3709 1310 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6031 BstBI TT'CG_AA Cuts at: 4835 4835 Size: 6031 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6031 BstXI CCAn_nnnn'nTGG Cuts at: 2824 2824 Size: 6031 BstYI r'GATC_y Cuts at: 1972 2813 3006 3361 3728 4113 4130 5582 5668 Size: 841 193 355 367 385 17 1452 86 Cuts at: 5668 5680 6026 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 841 385 367 355 346 193 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2349 2851 3142 513 Size: 552 939 345 502 291 3402 Fragments arranged by size: 3402 939 552 502 345 291 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5292 Fragments arranged by size: 5292 683 56 BtsI GCAGTG_nn' Cuts at: 3547 4341 4361 3547 Size: 794 20 5217 Fragments arranged by size: 5217 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6031 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3124 3159 529 Size: 35 2560 35 3401 Fragments arranged by size: 3401 2560 35 35 DraI TTT'AAA Cuts at: 2950 3335 2950 Size: 385 5646 DraIII CAC_nnn'GTG Cuts at: 3272 3272 Size: 6031 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5038 809 Size: 507 3722 1802 Fragments arranged by size: 3722 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3145 3478 4367 Size: 54 1425 50 275 18 1315 333 889 Cuts at: 4367 5996 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1315 889 333 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3478 1537 Size: 275 1666 4090 Fragments arranged by size: 4090 1666 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2108 2660 3961 1353 Size: 755 552 1301 3423 Fragments arranged by size: 3423 1301 755 552 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4618 4992 5138 387 Size: 1064 138 3029 374 146 1280 Fragments arranged by size: 3029 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2360 2588 4094 4691 5478 5746 1239 Size: 393 728 228 1506 597 787 268 1524 Fragments arranged by size: 1524 1506 787 728 597 393 268 228 EcoO109I rG'GnC_Cy Cuts at: 2954 3097 2954 Size: 143 5888 EcoRI G'AATT_C Cuts at: 2488 3340 3718 2488 Size: 852 378 4801 Fragments arranged by size: 4801 852 378 EcoRV GAT'ATC Cuts at: 2401 2401 Size: 6031 FspI TGC'GCA Cuts at: 4537 4537 Size: 6031 HaeII r_GCGC'y Cuts at: 1678 2134 5178 1678 Size: 456 3044 2531 Fragments arranged by size: 3044 2531 456 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2426 2458 2805 2837 2980 Size: 32 135 32 854 32 347 32 143 Cuts at: 2980 2998 3012 3030 3107 3139 4672 4704 4746 Size: 18 14 18 77 32 1533 32 42 Cuts at: 4746 4778 1373 Size: 32 2626 Fragments arranged by size: 2626 1533 854 347 143 135 77 42 32 32 32 32 32 32 32 18 18 14 HincII GTy'rAC Cuts at: 669 1657 1938 3375 3621 669 Size: 988 281 1437 246 3079 Fragments arranged by size: 3079 1437 988 281 246 HindIII A'AGCT_T Cuts at: 748 2807 748 Size: 2059 3972 HpaI GTT'AAC Cuts at: 3621 3621 Size: 6031 KpnI G_GTAC'C Cuts at: 3356 3356 Size: 6031 MfeI C'AATT_G Cuts at: 3630 3630 Size: 6031 MluI A'CGCG_T Cuts at: 5907 5907 Size: 6031 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2597 2841 5145 5329 1305 Size: 1292 244 2304 184 2007 Fragments arranged by size: 2304 2007 1292 244 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4209 Fragments arranged by size: 4209 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2241 3192 3989 4348 4507 518 Size: 648 1075 951 797 359 159 2042 Fragments arranged by size: 2042 1075 951 797 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2037 2273 2537 3447 4124 5272 5517 1606 Size: 431 236 264 910 677 1148 245 2120 Fragments arranged by size: 2120 1148 910 677 431 264 245 236 NaeI GCC'GGC Cuts at: 2024 2024 Size: 6031 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4540 Fragments arranged by size: 4540 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6031 NgoMIV G'CCGG_C Cuts at: 2022 2022 Size: 6031 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6031 NotI GC'GGCC_GC Cuts at: 3478 3478 Size: 6031 NspI r_CATG'y Cuts at: 1132 1229 2457 3389 4934 1132 Size: 97 1228 932 1545 2229 Fragments arranged by size: 2229 1545 1228 932 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6031 PciI A'CATG_T Cuts at: 4930 4930 Size: 6031 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6031 PfoI T'CCnGG_A Cuts at: 2625 2980 2625 Size: 355 5676 PmeI GTTT'AAAC Cuts at: 3335 3335 Size: 6031 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4100 4132 5613 5645 4100 Size: 32 1481 32 4486 Fragments arranged by size: 4486 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2954 2954 Size: 6031 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6031 PsiI TTA'TAA Cuts at: 3601 3601 Size: 6031 PstI C_TGCA'G Cuts at: 830 3383 830 Size: 2553 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6031 PvuII CAG'CTG Cuts at: 2273 2537 2273 Size: 264 5767 SalI G'TCGA_C Cuts at: 1936 3373 1936 Size: 1437 4594 SbfI CC_TGCA'GG Cuts at: 3383 3383 Size: 6031 ScaI AGT'ACT Cuts at: 4279 5991 4279 Size: 1712 4319 SfcI C'TryA_G Cuts at: 826 1045 3379 4514 5195 5386 826 Size: 219 2334 1135 681 191 1471 Fragments arranged by size: 2334 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6031 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6031 SmaI CCC'GGG Cuts at: 1872 3358 1872 Size: 1486 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2108 2135 2595 3046 4135 5036 Size: 197 926 165 27 460 451 1089 901 Cuts at: 5036 5298 820 Size: 262 1553 Fragments arranged by size: 1553 1089 926 901 460 451 262 197 165 27 SnaBI TAC'GTA Cuts at: 493 3822 493 Size: 3329 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6031 SphI G_CATG'C Cuts at: 1132 3389 1132 Size: 2257 3774 SspI AAT'ATT Cuts at: 5 52 3955 5 Size: 47 3903 2081 Fragments arranged by size: 3903 2081 47 StuI AGG'CCT Cuts at: 1517 3284 1517 Size: 1767 4264 StyI C'CwwG_G Cuts at: 513 1065 2004 2189 2214 2957 3470 513 Size: 552 939 185 25 743 513 3074 Fragments arranged by size: 3074 939 743 552 513 185 25 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3284 4058 4075 4228 4413 4858 325 Size: 1255 1704 774 17 153 185 445 1498 Fragments arranged by size: 1704 1498 1255 774 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2673 3277 4277 Size: 274 80 33 51 1443 696 604 1000 Cuts at: 4277 5989 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1000 696 604 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5991 6023 5991 Size: 32 5999 Tth111I GACn'n_nGTC Cuts at: 1316 2045 1316 Size: 729 5302 XbaI T'CTAG_A Cuts at: 3367 3367 Size: 6031 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6031 XmaI C'CCGG_G Cuts at: 1870 3356 1870 Size: 1486 4545 XmnI GAAnn'nnTTC Cuts at: 3173 4160 3173 Size: 987 5044 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3839 276 Size: 53 83 186 936 2305 2468 Fragments arranged by size: 2468 2305 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI