Restriction map for
pFN21AB7747
(Linear) (Six-base) MAPSORT of: KIBB7747.seq Check: 2758 from: 1 to: 1326
KIBB7747.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 1326
Size: 355 971
AflIII A'CryG_T
Cuts at: 0 1221 1235 1326
Size: 1221 14 91
Fragments arranged by size:
1221 91 14
AhdI GACnn_n'nnGTC
Cuts at: 0 1116 1326
Size: 1116 210
AlwNI CAG_nnn'CTG
Cuts at: 0 271 906 1326
Size: 271 635 420
Fragments arranged by size:
635 420 271
ApoI r'AATT_y
Cuts at: 0 483 1326
Size: 483 843
AvrII C'CTAG_G
Cuts at: 0 952 1326
Size: 952 374
BbsI GAAGACnn'nnnn_
Cuts at: 0 36 1326
Size: 36 1290
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 277 311 1326
Size: 277 34 1015
Fragments arranged by size:
1015 277 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 342 1326
Size: 342 984
BclI T'GATC_A
Cuts at: 0 968 1326
Size: 968 358
BlpI GC'TnA_GC
Cuts at: 0 77 1326
Size: 77 1249
BmgBI CAC'GTC
Cuts at: 0 1222 1236 1326
Size: 1222 14 90
Fragments arranged by size:
1222 90 14
BmrI ACTGGGnnnn_n'
Cuts at: 0 435 1326
Size: 435 891
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 583 1326
Size: 583 743
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 151 575 1026 1326
Size: 124 27 424 451 300
Fragments arranged by size:
451 424 300 124 27
BsaI GGTCTCn'nnnn_
Cuts at: 0 652 1326
Size: 652 674
BsaHI Gr'CG_yC
Cuts at: 0 1156 1326
Size: 1156 170
BsaWI w'CCGG_w
Cuts at: 0 505 1326
Size: 505 821
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 736 766 1326
Size: 736 30 560
Fragments arranged by size:
736 560 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 303 436 1326
Size: 303 133 890
Fragments arranged by size:
890 303 133
BseYI C'CCAG_C
Cuts at: 0 28 1326
Size: 28 1298
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 1066 1289 1326
Size: 166 900 223 37
Fragments arranged by size:
900 223 166 37
BsmBI CGTCTCn'nnnn_
Cuts at: 0 385 1326
Size: 385 941
BsrBI CCG'CTC
Cuts at: 0 886 1326
Size: 886 440
BsrDI GCAATG_nn'
Cuts at: 0 243 1326
Size: 243 1083
BsrFI r'CCGG_y
Cuts at: 0 17 1326
Size: 17 1309
BssSI C'ACGA_G
Cuts at: 0 776 1326
Size: 776 550
BstXI CCAn_nnnn'nTGG
Cuts at: 0 819 1326
Size: 819 507
BstYI r'GATC_y
Cuts at: 0 808 1001 1326
Size: 808 193 325
Fragments arranged by size:
808 325 193
BtgI C'CryG_G
Cuts at: 0 344 846 1137 1326
Size: 344 502 291 189
Fragments arranged by size:
502 344 291 189
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1119 1154 1326
Size: 1119 35 172
Fragments arranged by size:
1119 172 35
DraI TTT'AAA
Cuts at: 0 945 1326
Size: 945 381
DraIII CAC_nnn'GTG
Cuts at: 0 1267 1326
Size: 1267 59
EaeI y'GGCC_r
Cuts at: 0 1140 1326
Size: 1140 186
EarI CTCTTCn'nnn_
Cuts at: 0 103 655 1326
Size: 103 552 671
Fragments arranged by size:
671 552 103
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 583 1326
Size: 355 228 743
Fragments arranged by size:
743 355 228
EcoO109I rG'GnC_Cy
Cuts at: 0 949 1092 1326
Size: 949 143 234
Fragments arranged by size:
949 234 143
EcoRI G'AATT_C
Cuts at: 0 483 1326
Size: 483 843
EcoRV GAT'ATC
Cuts at: 0 396 1326
Size: 396 930
HaeII r_GCGC'y
Cuts at: 0 129 1326
Size: 129 1197
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 421 453 800 832 975 993 1007 1025
Size: 421 32 347 32 143 18 14 18
Cuts at: 1025 1102 1134 1326
Size: 77 32 192
Fragments arranged by size:
421 347 192 143 77 32 32 32
18 18 14
HindIII A'AGCT_T
Cuts at: 0 802 1326
Size: 802 524
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 836 1326
Size: 592 244 490
Fragments arranged by size:
592 490 244
MslI CAynn'nnrTG
Cuts at: 0 236 1187 1326
Size: 236 951 139
Fragments arranged by size:
951 236 139
MspA1I CmG'CkG
Cuts at: 0 32 268 532 1326
Size: 32 236 264 794
Fragments arranged by size:
794 264 236 32
NaeI GCC'GGC
Cuts at: 0 19 1326
Size: 19 1307
NgoMIV G'CCGG_C
Cuts at: 0 17 1326
Size: 17 1309
NspI r_CATG'y
Cuts at: 0 452 1326
Size: 452 874
PfoI T'CCnGG_A
Cuts at: 0 620 975 1326
Size: 620 355 351
Fragments arranged by size:
620 355 351
PpuMI rG'GwC_Cy
Cuts at: 0 949 1326
Size: 949 377
PvuII CAG'CTG
Cuts at: 0 268 532 1326
Size: 268 264 794
Fragments arranged by size:
794 268 264
SmlI C'TyrA_G
Cuts at: 0 103 130 590 1041 1326
Size: 103 27 460 451 285
Fragments arranged by size:
460 451 285 103 27
StuI AGG'CCT
Cuts at: 0 1279 1326
Size: 1279 47
StyI C'CwwG_G
Cuts at: 0 184 209 952 1326
Size: 184 25 743 374
Fragments arranged by size:
743 374 184 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1279 1326
Size: 1279 47
TatI w'GTAC_w
Cuts at: 0 668 1272 1326
Size: 668 604 54
Fragments arranged by size:
668 604 54
Tth111I GACn'n_nGTC
Cuts at: 0 40 1326
Size: 40 1286
XmnI GAAnn'nnTTC
Cuts at: 0 1168 1326
Size: 1168 158
Enzymes that do cut:
AcuI AflIII AhdI AlwNI ApoI AvrII BbsI BcgI
BciVI BclI BlpI BmgBI BmrI BpmI BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsmBI BsrBI
BsrDI BsrFI BssSI BstXI BstYI BtgI CspCI DraI
DraIII EaeI EarI Eco57MI EcoO109I EcoRI EcoRV HaeII
Hin4I HindIII MmeI MslI MspA1I NaeI NgoMIV NspI
PfoI PpuMI PvuII SmlI StuI StyI TaqII TatI
Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbvCI BfrBI
BglI BglII Bme1580I BmtI BplI Bpu10I BsaAI BsaBI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI
BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI
BtsI ClaI DrdI EagI EciI EcoICRI EcoNI FalI
FseI FspI FspAI HincII HpaI KasI KpnI MfeI
MluI MscI NarI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TstI XbaI XcmI XhoI
XmaI ZraI