Restriction map for
pFN21AB7736
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccII AccIII AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AvaI AviII
AvrII BamHI BanII BbeI BbrPI
BcgI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsoBI BspDI BspEI BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I CelII ClaI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoT22I FbaI FseI FspI
HaeII HindIII KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI MwoI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI PstI PvuI
SacI SacII SalI SapI SbfI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI SwaI XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 336 GTCTAC GT^[AC][GT]AC
AcyI 1159 GACGCC G[GA]^CG[CT]C
AflIII 248 ACATGT A^C[GA][CT]GT
BalI 473 TGGCCA TGG^CCA
BanI 16 GGCACC G^G[CT][GA]CC
BbsI 1192 GAAGAC GAAGAC(2/6)
BbvCI 181 GCTGAGG CCTCAGC(-5/-2)
BciVI 960 GGATAC GTATCC(6/5)
BfaI 761 CTAG C^TAG
BmyI 15 GGGCAC G[AGT]GC[ACT]^C
BpmI 1075 CTGGAG CTGGAG(16/14)
BpuAI 1192 GAAGAC GAAGAC(2/6)
BsaHI 1159 GACGCC G[GA]^CG[CT]C
BsaI 136 GGTCTC GGTCTC(1/5)
BsgI 415 CTGCAC GTGCAG(16/14)
BsmBI 686 GAGACG CGTCTC(1/5)
BsmFI 626 GGGAC GGGAC(10/14)
BsmI 1112 GAATGC GAATGC(1/-1)
Bsp1286I 15 GGGCAC G[AGT]GC[ACT]^C
Bsp1407I 512 TGTACA T^GTACA
BspHI 578 TCATGA T^CATGA
BspLU11I 248 ACATGT A^CATGT
BspMI 875 ACCTGC ACCTGC(4/8)
BsrBI 679 CCGCTC CCGCTC(-3/-3)
BsrFI 726 GCCGGT [GA]^CCGG[CT]
BsrGI 512 TGTACA T^GTACA
BstYI 968 AGATCC [GA]^GATC[CT]
Cfr10I 726 GCCGGT [GA]^CCGG[CT]
CpoI 569 CGGACCG CG^G[AT]CCG
EaeI 473 TGGCCA [CT]^GGCC[GA]
FauI 678 CCCGC CCCGC(4/6)
Hin1I 1159 GACGCC G[GA]^CG[CT]C
HincII 670 GTTAAC GT[CT]^[GA]AC
HindII 670 GTTAAC GT[CT]^[GA]AC
HpaI 670 GTTAAC GTT^AAC
MaeI 761 CTAG C^TAG
MflI 968 AGATCC [GA]^GATC[CT]
MluNI 473 TGGCCA TGG^CCA
MscI 473 TGGCCA TGG^CCA
MspA1I 947 CAGCTG C[AC]G^C[GT]G
NcoI 534 CCATGG C^CATGG
NdeI 938 CATATG CA^TATG
NspI 248 ACATGT [GA]CATG^[CT]
PflMI 616 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 626 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 947 CAGCTG CAG^CTG
RcaI 578 TCATGA T^CATGA
RsrII 569 CGGACCG CG^G[AT]CCG
ScaI 608 AGTACT AGT^ACT
SspBI 512 TGTACA T^GTACA
StuI 554 AGGCCT AGG^CCT
TspRI 778 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 616 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 968 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AclI 283 AACGTT AA^CGTT
919 AACGTT
AcsI 220 AAATTC [GA]^AATT[CT]
856 GAATTT
AlwI 969 GATCC GGATC(4/5)
804 GGATC
AlwNI 617 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
1090 CAGCGACTG
AocI 1153 CCTGAGG CC^T[ACGT]AGG
1168 CCTGAGG
ApoI 220 AAATTC [GA]^AATT[CT]
856 GAATTT
AspI 571 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
582 GACTGTGTC
BstXI 737 CCAAAGTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
845 CCAAGTGTGTGG
Bsu36I 1153 CCTGAGG CC^T[ACGT]AGG
1168 CCTGAGG
DraII 410 GGGGCCT [GA]G^G[ACGT]CC[CT]
626 GGGACCT
DsaI 534 CCATGG C^C[GA][CT]GG
566 CCACGG
Eco81I 1153 CCTGAGG CC^T[ACGT]AGG
1168 CCTGAGG
EcoO109I 410 GGGGCCT [GA]G^G[ACGT]CC[CT]
626 GGGACCT
EcoRV 463 GATATC GAT^ATC
637 GATATC
EcoT14I 534 CCATGG C^C[AT][AT]GG
1394 CCAAGG
HgaI 9 GACGC GACGC(5/10)
1159 GACGC
PflFI 571 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
582 GACTGTGTC
Psp1406I 283 AACGTT AA^CGTT
919 AACGTT
StyI 534 CCATGG C^C[AT][AT]GG
1394 CCAAGG
TaqI 308 TCGA T^CGA
1216 TCGA
Tsp45I 539 GTGAC ^GT[GC]AC
1375 GTCAC
Tth111I 571 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
582 GACTGTGTC
TthHB8I 308 TCGA T^CGA
1216 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BcnI BsaJI BseRI BsiYI BslI
BsmAI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI TfiI Tru9I TseI
Tsp509I