Restriction map for
pFN21AB7730
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AvrII BalI BbrPI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsiWI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrGI BsrI BssSI Bst1107I BstBI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI HindIII KpnI
MamI MfeI MluI MluNI MscI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SalI SbfI ScaI SfaNI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspBI SspI SwaI TfiI XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 136 GACGTC GACGT^C
AccIII 1345 TCCGGA T^CCGGA
AspHI 459 GAGCTC G[AT]GC[AT]^C
AviII 54 TGCGCA TGC^GCA
BamHI 1175 GGATCC G^GATCC
BbvCI 383 CCTCAGC CCTCAGC(-5/-2)
BseAI 1345 TCCGGA T^CCGGA
BsiHKAI 459 GAGCTC G[AT]GC[AT]^C
BsmI 51 GAATGC GAATGC(1/-1)
BspEI 1345 TCCGGA T^CCGGA
BsrDI 947 GCAATG GCAATG(2/0)
BssHII 202 GCGCGC G^CGCGC
BstAPI 111 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1425 GGTGACC G^GT[ACGT]ACC
BstPI 1425 GGTGACC G^GT[ACGT]ACC
BstYI 1175 GGATCC [GA]^GATC[CT]
DpnII 1176 GATC ^GATC
EcoO65I 1425 GGTGACC G^GT[ACGT]ACC
EcoT14I 1433 CCATGG C^C[AT][AT]GG
FspI 54 TGCGCA TGC^GCA
HpaI 1144 GTTAAC GTT^AAC
KspI 590 CCGCGG CCGC^GG
MaeII 137 ACGT A^CGT
MboI 1176 GATC ^GATC
MflI 1175 GGATCC [GA]^GATC[CT]
MroI 1345 TCCGGA T^CCGGA
MseI 1145 TTAA T^TAA
MslI 660 CATGAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1433 CCATGG C^CATGG
NdeII 1176 GATC ^GATC
NspI 1259 GCATGC [GA]CATG^[CT]
PpuMI 1097 GGGACCC [GA]G^G[AT]CC[CT]
SacI 459 GAGCTC GAGCT^C
SacII 590 CCGCGG CCGC^GG
Sau3AI 1176 GATC ^GATC
SexAI 638 ACCAGGT A^CC[AT]GGT
SfiI 397 GGCCCTGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1259 GCATGC GCATG^C
StuI 693 AGGCCT AGG^CCT
StyI 1433 CCATGG C^C[AT][AT]GG
Tru9I 1145 TTAA T^TAA
Tsp509I 1051 AATT ^AATT
XcmI 966 CCACAAGATGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1175 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AfaI 420 GTAC GT^AC
636 GTAC
AlwI 1176 GATCC GGATC(4/5)
1175 GGATC
AspI 625 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1099 GACCCTGTC
BanII 354 GAGCCC G[GA]GC[CT]^C
459 GAGCTC
BbeI 9 GGCGCC GGCGC^C
1079 GGCGCC
BbsI 1010 GAAGAC GAAGAC(2/6)
139 GTCTTC
BfaI 554 CTAG C^TAG
697 CTAG
BglI 319 GCCGCTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
398 GCCCTGGAGGC
BpuAI 1010 GAAGAC GAAGAC(2/6)
139 GTCTTC
BsiEI 41 CGGCCG CG[GA][CT]^CG
1019 CGGCCG
BsmBI 207 CGTCTC CGTCTC(1/5)
572 CGTCTC
BsrBI 188 CCGCTC CCGCTC(-3/-3)
441 GAGCGG
DraII 396 AGGCCCT [GA]G^G[ACGT]CC[CT]
1097 GGGACCC
EagI 41 CGGCCG C^GGCCG
1019 CGGCCG
EclXI 41 CGGCCG C^GGCCG
1019 CGGCCG
Eco52I 41 CGGCCG C^GGCCG
1019 CGGCCG
EcoO109I 396 AGGCCCT [GA]G^G[ACGT]CC[CT]
1097 GGGACCC
HaeII 9 GGCGCC [GA]GCGC^[CT]
1079 GGCGCC
HincII 886 GTCAAC GT[CT]^[GA]AC
1144 GTTAAC
HindII 886 GTCAAC GT[CT]^[GA]AC
1144 GTTAAC
KasI 9 GGCGCC G^GCGCC
1079 GGCGCC
MaeI 554 CTAG C^TAG
697 CTAG
MaeIII 645 GTCAC ^GT[ACGT]AC
1426 GTGAC
NaeI 759 GCCGGC GCC^GGC
1033 GCCGGC
NarI 9 GGCGCC GG^CGCC
1079 GGCGCC
NgoMIV 759 GCCGGC G^CCGGC
1033 GCCGGC
PflFI 625 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1099 GACCCTGTC
PflMI 424 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1070 CCACCTTATGG
RsaI 420 GTAC GT^AC
636 GTAC
SapI 1028 GAAGAGC GCTCTTC(1/4)
1215 GAAGAGC
SfoI 9 GGCGCC GGC^GCC
1079 GGCGCC
SmaI 78 CCCGGG CCC^GGG
276 CCCGGG
TaqI 155 TCGA T^CGA
1241 TCGA
Tsp45I 645 GTCAC ^GT[GC]AC
1426 GTGAC
Tth111I 625 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1099 GACCCTGTC
TthHB8I 155 TCGA T^CGA
1241 TCGA
Van91I 424 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1070 CCACCTTATGG
XmaCI 78 CCCGGG C^CCGGG
276 CCCGGG
XmaI 78 CCCGGG C^CCGGG
276 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
AvaI AvaII BanI BbvI BcnI
BmyI BpmI BsaHI BsaJI BsaWI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DsaI EaeI
EarI EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV PleI PvuII Sau96I ScrFI
SfcI TseI TspRI