(Circular) (Six-base) MAPSORT of: pFN21AB7730.seq Check: 1268 from: 1 to: 6145 pFN21AB7730 6145 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2145 3955 278 Size: 53 83 186 936 609 1810 2468 Fragments arranged by size: 2468 1810 936 609 186 83 53 AccI GT'mk_AC Cuts at: 1937 3488 1937 Size: 1551 4594 Acc65I G'GTAC_C Cuts at: 3466 3466 Size: 6145 AclI AA'CG_TT Cuts at: 1563 4272 4645 1563 Size: 2709 373 3063 Fragments arranged by size: 3063 2709 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3271 3325 4208 5592 5860 1632 Size: 1639 54 883 1384 268 1917 Fragments arranged by size: 1917 1639 1384 883 268 54 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5948 AflIII A'CryG_T Cuts at: 1203 5044 6021 1203 Size: 3841 977 1327 Fragments arranged by size: 3841 1327 977 AgeI A'CCGG_T Cuts at: 4976 4976 Size: 6145 AhdI GACnn_n'nnGTC Cuts at: 4874 4874 Size: 6145 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6145 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2705 1184 Size: 1521 4624 AlwNI CAG_nnn'CTG Cuts at: 2122 2396 2435 3888 5460 2122 Size: 274 39 1453 1572 2807 Fragments arranged by size: 2807 1572 1453 274 39 ApaLI G'TGCA_C Cuts at: 4202 5358 4202 Size: 1156 4989 ApoI r'AATT_y Cuts at: 1493 3454 3680 3832 5941 1493 Size: 1961 226 152 2109 1697 Fragments arranged by size: 2109 1961 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4699 160 Size: 872 3667 1606 Fragments arranged by size: 3667 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6145 AvaI C'yCGr_G Cuts at: 1870 1943 2018 2083 2281 2613 3470 1870 Size: 73 75 65 198 332 857 4545 Fragments arranged by size: 4545 857 332 198 75 73 65 BamHI G'GATC_C Cuts at: 3180 3475 3842 3180 Size: 295 367 5483 Fragments arranged by size: 5483 367 295 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2014 2714 2933 3041 3084 Size: 325 217 627 227 700 219 108 43 Cuts at: 3084 3316 3466 4921 618 Size: 232 150 1455 1842 Fragments arranged by size: 1842 1455 700 627 325 232 227 219 217 150 108 43 BanII G_rGCy'C Cuts at: 1391 2363 2468 3464 1391 Size: 972 105 996 4072 Fragments arranged by size: 4072 996 972 105 BbeI G_GCGC'C Cuts at: 2018 3088 2018 Size: 1070 5075 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2137 3022 928 Size: 971 238 885 4051 Fragments arranged by size: 4051 971 885 238 BbvCI CC'TCA_GC Cuts at: 2389 2389 Size: 6145 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4336 4370 1903 Size: 34 2399 34 3678 Fragments arranged by size: 3678 2399 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4037 5247 4037 Size: 1210 4935 BclI T'GATC_A Cuts at: 1548 1548 Size: 6145 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2330 2409 4756 Size: 107 122 71 631 605 658 79 2347 Cuts at: 4756 136 Size: 1525 Fragments arranged by size: 2347 1525 658 631 605 122 107 79 71 BglII A'GATC_T Cuts at: 6140 6140 Size: 6145 BlpI GC'TnA_GC Cuts at: 3562 3562 Size: 6145 Bme1580I G_kGCm'C Cuts at: 1790 2717 4206 5362 1790 Size: 927 1489 1156 2573 Fragments arranged by size: 2573 1489 1156 927 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6145 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3876 4834 442 Size: 416 53 83 399 24 2459 958 1753 Fragments arranged by size: 2459 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6145 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2432 2432 Size: 6145 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2112 2418 2427 2565 2815 3376 4805 1239 Size: 873 306 9 138 250 561 1429 2579 Fragments arranged by size: 2579 1429 873 561 306 250 138 9 Bpu10I CC'TnA_GC Cuts at: 2389 2844 2389 Size: 455 5690 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4270 5135 5433 4270 Size: 865 298 4982 Fragments arranged by size: 4982 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4808 882 Size: 617 3309 2219 Fragments arranged by size: 3309 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3936 493 Size: 711 2732 2702 Fragments arranged by size: 2732 2702 711 BsaBI GATnn'nnATC Cuts at: 6139 6139 Size: 6145 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2015 2142 2375 2634 Size: 53 83 186 936 482 127 233 259 Cuts at: 2634 3085 3374 3952 4334 4969 275 Size: 451 289 578 382 635 1451 Fragments arranged by size: 1451 936 635 578 482 451 382 289 259 233 186 127 83 53 BsaWI w'CCGG_w Cuts at: 2243 3195 3350 4578 4976 5250 5397 5808 2243 Size: 952 155 1228 398 274 147 411 2580 Fragments arranged by size: 2580 1228 952 411 398 274 155 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2489 2519 3374 3404 1178 Size: 30 165 30 1086 30 855 30 3919 Fragments arranged by size: 3919 1086 855 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2631 3263 3379 1186 Size: 1445 632 116 3952 Fragments arranged by size: 3952 1445 632 116 BseYI C'CCAG_C Cuts at: 2435 2718 5348 5844 2435 Size: 283 2630 496 2736 Fragments arranged by size: 2736 2630 496 283 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2366 2628 3125 3312 1163 Size: 573 630 262 497 187 3996 Fragments arranged by size: 3996 630 573 497 262 187 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2049 3027 3595 4356 5384 1540 Size: 275 186 48 978 568 761 1028 2301 Fragments arranged by size: 2301 1028 978 761 568 275 186 48 BsiHKAI G_wGCw'C Cuts at: 2468 4206 4291 5362 2468 Size: 1738 85 1071 3251 Fragments arranged by size: 3251 1738 1071 85 BsmI GAATG_Cn' Cuts at: 2062 3656 3749 2062 Size: 1594 93 4458 Fragments arranged by size: 4458 1594 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2218 2583 2218 Size: 365 5780 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2363 2468 2717 3464 4206 4291 5362 Size: 399 573 105 249 747 742 85 1071 Cuts at: 5362 1391 Size: 2174 Fragments arranged by size: 2174 1071 747 742 573 399 249 105 85 BspEI T'CCGG_A Cuts at: 3350 5808 3350 Size: 2458 3687 BspHI T'CATG_A Cuts at: 4034 5764 4034 Size: 1730 4415 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3500 844 Size: 2656 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2195 2448 4032 1394 Size: 429 372 253 1584 3507 Fragments arranged by size: 3507 1584 429 372 253 BsrDI GCAATG_nn' Cuts at: 65 1248 2959 4640 4814 65 Size: 1183 1711 1681 174 1396 Fragments arranged by size: 1711 1681 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2764 2824 2936 3038 4789 4976 1700 Size: 110 954 60 112 102 1751 187 2869 Fragments arranged by size: 2869 1751 954 187 112 110 102 60 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6145 BssHII G'CGCG_C Cuts at: 1925 2207 1925 Size: 282 5863 BssSI C'ACGA_G Cuts at: 4205 5217 4205 Size: 1012 5133 BstAPI GCAn_nnn'nTGC Cuts at: 794 2122 794 Size: 1328 4817 BstBI TT'CG_AA Cuts at: 4949 4949 Size: 6145 BstEII G'GTnAC_C Cuts at: 1184 3430 1184 Size: 2246 3899 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6145 BstYI r'GATC_y Cuts at: 1972 3180 3475 3842 4227 4244 5696 5782 5794 Size: 1208 295 367 385 17 1452 86 12 Cuts at: 5794 6140 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1208 385 367 346 295 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2565 2595 3438 513 Size: 552 939 561 30 843 3220 Fragments arranged by size: 3220 939 843 561 552 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5406 Fragments arranged by size: 5406 683 56 BtsI GCAGTG_nn' Cuts at: 2378 3661 4455 4475 2378 Size: 1283 794 20 4048 Fragments arranged by size: 4048 1283 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6145 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2253 2288 2941 2976 3103 3138 529 Size: 35 1689 35 653 35 127 35 3536 Fragments arranged by size: 3536 1689 653 127 35 35 35 35 DraI TTT'AAA Cuts at: 3449 3449 Size: 6145 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5152 809 Size: 507 3836 1802 Fragments arranged by size: 3836 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2938 3024 Size: 54 1425 50 275 18 216 892 86 Cuts at: 3024 3406 3592 4481 6110 8 Size: 382 186 889 1629 43 Fragments arranged by size: 1629 1425 892 889 382 275 216 186 86 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2046 3024 3592 1537 Size: 275 234 978 568 4090 Fragments arranged by size: 4090 978 568 275 234 EarI CTCTTCn'nnn_ Cuts at: 1353 2801 3028 3215 4075 1353 Size: 1448 227 187 860 3423 Fragments arranged by size: 3423 1448 860 227 187 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3251 4732 5106 5252 387 Size: 1064 138 1662 1481 374 146 1280 Fragments arranged by size: 1662 1481 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2466 2466 Size: 6145 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2112 2418 2427 2565 2815 3271 3325 Size: 393 480 306 9 138 250 456 54 Cuts at: 3325 3376 4208 4805 5592 5860 1239 Size: 51 832 597 787 268 1524 Fragments arranged by size: 1524 832 787 597 480 456 393 306 268 250 138 54 51 9 EcoO109I rG'GnC_Cy Cuts at: 2402 3103 2402 Size: 701 5444 EcoRI G'AATT_C Cuts at: 3454 3832 3454 Size: 378 5767 FspI TGC'GCA Cuts at: 2061 4651 2061 Size: 2590 3555 HaeII r_GCGC'y Cuts at: 1678 2018 3088 5292 1678 Size: 340 1070 2204 2531 Fragments arranged by size: 2531 2204 1070 340 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2985 3017 3172 3204 3239 Size: 32 135 32 1413 32 155 32 35 Cuts at: 3239 3263 3271 3295 4786 4818 4860 4892 1373 Size: 24 8 24 1491 32 42 32 2626 Fragments arranged by size: 2626 1491 1413 155 135 42 35 32 32 32 32 32 32 24 24 8 HincII GTy'rAC Cuts at: 669 1657 1938 2893 3151 3489 3735 669 Size: 988 281 955 258 338 246 3079 Fragments arranged by size: 3079 988 955 338 281 258 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6145 HpaI GTT'AAC Cuts at: 3151 3735 3151 Size: 584 5561 KasI G'GCGC_C Cuts at: 2014 3084 2014 Size: 1070 5075 KpnI G_GTAC'C Cuts at: 3470 3470 Size: 6145 MfeI C'AATT_G Cuts at: 3744 3744 Size: 6145 MluI A'CGCG_T Cuts at: 6021 6021 Size: 6145 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3231 3395 5259 5443 1305 Size: 1926 164 1864 184 2007 Fragments arranged by size: 2007 1926 1864 184 164 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4323 Fragments arranged by size: 4323 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2669 4103 4462 4621 518 Size: 648 843 660 1434 359 159 2042 Fragments arranged by size: 2042 1434 843 660 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2119 2327 2393 2460 2597 2790 3296 3561 Size: 513 208 66 67 137 193 506 265 Cuts at: 3561 4238 5386 5631 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 513 506 265 245 208 193 137 67 66 NaeI GCC'GGC Cuts at: 2766 3040 2766 Size: 274 5871 NarI GG'CG_CC Cuts at: 2015 3085 2015 Size: 1070 5075 NcoI C'CATG_G Cuts at: 513 1065 2004 3438 513 Size: 552 939 1434 3220 Fragments arranged by size: 3220 1434 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6145 NgoMIV G'CCGG_C Cuts at: 2764 3038 2764 Size: 274 5871 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6145 NotI GC'GGCC_GC Cuts at: 3592 3592 Size: 6145 NspI r_CATG'y Cuts at: 1132 1229 3268 3503 5048 1132 Size: 97 2039 235 1545 2229 Fragments arranged by size: 2229 2039 1545 235 97 PasI CC'CwG_GG Cuts at: 1348 2429 1348 Size: 1081 5064 PciI A'CATG_T Cuts at: 5044 5044 Size: 6145 PflMI CCAn_nnn'nTGG Cuts at: 1108 2435 3081 1108 Size: 1327 646 4172 Fragments arranged by size: 4172 1327 646 PmeI GTTT'AAAC Cuts at: 3449 3449 Size: 6145 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4214 4246 5727 5759 4214 Size: 32 1481 32 4600 Fragments arranged by size: 4600 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3103 3103 Size: 6145 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6145 PsiI TTA'TAA Cuts at: 3715 3715 Size: 6145 PstI C_TGCA'G Cuts at: 830 3497 830 Size: 2667 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6145 PvuII CAG'CTG Cuts at: 2119 2393 3296 2119 Size: 274 903 4968 Fragments arranged by size: 4968 903 274 SacI G_AGCT'C Cuts at: 2468 2468 Size: 6145 SacII CC_GC'GG Cuts at: 2598 2598 Size: 6145 SalI G'TCGA_C Cuts at: 1936 3487 1936 Size: 1551 4594 SanDI GG'GwC_CC Cuts at: 3103 3103 Size: 6145 SapI GCTCTTCn'nnn_ Cuts at: 3028 3215 3028 Size: 187 5958 SbfI CC_TGCA'GG Cuts at: 3497 3497 Size: 6145 ScaI AGT'ACT Cuts at: 4393 6105 4393 Size: 1712 4433 SexAI A'CCwGG_T Cuts at: 2643 2643 Size: 6145 SfcI C'TryA_G Cuts at: 826 1045 2218 2487 2782 3493 4628 5309 5500 Size: 219 1173 269 295 711 1135 681 191 Cuts at: 5500 826 Size: 1471 Fragments arranged by size: 1471 1173 1135 711 681 295 269 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2409 2409 Size: 6145 SfoI GGC'GCC Cuts at: 2016 3086 2016 Size: 1070 5075 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6145 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6145 SmaI CCC'GGG Cuts at: 1872 2085 2283 3472 1872 Size: 213 198 1189 4545 Fragments arranged by size: 4545 1189 213 198 SmlI C'TyrA_G Cuts at: 820 1017 1943 4249 5150 5412 820 Size: 197 926 2306 901 262 1553 Fragments arranged by size: 2306 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3936 493 Size: 3443 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6145 SphI G_CATG'C Cuts at: 1132 3268 3503 1132 Size: 2136 235 3774 Fragments arranged by size: 3774 2136 235 SspI AAT'ATT Cuts at: 5 52 4069 5 Size: 47 4017 2081 Fragments arranged by size: 4017 2081 47 StuI AGG'CCT Cuts at: 1517 2700 1517 Size: 1183 4962 StyI C'CwwG_G Cuts at: 513 1065 2004 3438 3584 513 Size: 552 939 1434 146 3074 Fragments arranged by size: 3074 1434 939 552 146 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2542 2551 2732 4172 4189 4342 4527 Size: 1255 962 9 181 1440 17 153 185 Cuts at: 4527 4972 325 Size: 445 1498 Fragments arranged by size: 1498 1440 1255 962 445 185 181 153 17 9 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4391 6103 96 Size: 274 80 33 51 1443 2414 1712 138 Fragments arranged by size: 2414 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2489 2521 3374 3406 6105 6137 2489 Size: 32 853 32 2699 32 2497 Fragments arranged by size: 2699 2497 853 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2633 3107 1316 Size: 1317 474 4354 Fragments arranged by size: 4354 1317 474 XbaI T'CTAG_A Cuts at: 3481 3481 Size: 6145 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2978 2978 Size: 6145 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6145 XmaI C'CCGG_G Cuts at: 1870 2083 2281 3470 1870 Size: 213 198 1189 4545 Fragments arranged by size: 4545 1189 213 198 XmnI GAAnn'nnTTC Cuts at: 4274 4274 Size: 6145 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2143 3953 276 Size: 53 83 186 936 609 1810 2468 Fragments arranged by size: 2468 1810 936 609 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SrfI SwaI