Restriction map for
pFN21AB7730
(Linear) (Six-base) MAPSORT of: KIBB7730.seq Check: 9429 from: 1 to: 1440
KIBB7730.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AatII G_ACGT'C
Cuts at: 0 140 1440
Size: 140 1300
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1266 1320 1440
Size: 1266 54 120
Fragments arranged by size:
1266 120 54
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 700 1440
Size: 700 740
AlwNI CAG_nnn'CTG
Cuts at: 0 117 391 430 1440
Size: 117 274 39 1010
Fragments arranged by size:
1010 274 117 39
AvaI C'yCGr_G
Cuts at: 0 13 78 276 608 1440
Size: 13 65 198 332 832
Fragments arranged by size:
832 332 198 65 13
BamHI G'GATC_C
Cuts at: 0 1175 1440
Size: 1175 265
BanI G'GyrC_C
Cuts at: 0 9 709 928 1036 1079 1311 1440
Size: 9 700 219 108 43 232 129
Fragments arranged by size:
700 232 219 129 108 43 9
BanII G_rGCy'C
Cuts at: 0 358 463 1440
Size: 358 105 977
Fragments arranged by size:
977 358 105
BbeI G_GCGC'C
Cuts at: 0 13 1083 1440
Size: 13 1070 357
Fragments arranged by size:
1070 357 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 132 1017 1440
Size: 132 885 423
Fragments arranged by size:
885 423 132
BbvCI CC'TCA_GC
Cuts at: 0 384 1440
Size: 384 1056
BglI GCCn_nnn'nGGC
Cuts at: 0 325 404 1440
Size: 325 79 1036
Fragments arranged by size:
1036 325 79
Bme1580I G_kGCm'C
Cuts at: 0 712 1440
Size: 712 728
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 427 1440
Size: 427 1013
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 413 422 560 810 1371 1440
Size: 107 306 9 138 250 561 69
Fragments arranged by size:
561 306 250 138 107 69 9
Bpu10I CC'TnA_GC
Cuts at: 0 384 839 1440
Size: 384 455 601
Fragments arranged by size:
601 455 384
BsaHI Gr'CG_yC
Cuts at: 0 10 137 370 629 1080 1369 1440
Size: 10 127 233 259 451 289 71
Fragments arranged by size:
451 289 259 233 127 71 10
BsaWI w'CCGG_w
Cuts at: 0 238 1190 1345 1440
Size: 238 952 155 95
Fragments arranged by size:
952 238 155 95
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 484 514 1369 1399 1440
Size: 484 30 855 30 41
Fragments arranged by size:
855 484 41 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 626 1258 1374 1440
Size: 626 632 116 66
Fragments arranged by size:
632 626 116 66
BseYI C'CCAG_C
Cuts at: 0 430 713 1440
Size: 430 283 727
Fragments arranged by size:
727 430 283
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 623 1120 1307 1440
Size: 361 262 497 187 133
Fragments arranged by size:
497 361 262 187 133
BsiEI CG_ry'CG
Cuts at: 0 44 1022 1440
Size: 44 978 418
Fragments arranged by size:
978 418 44
BsiHKAI G_wGCw'C
Cuts at: 0 463 1440
Size: 463 977
BsmI GAATG_Cn'
Cuts at: 0 57 1440
Size: 57 1383
BsmBI CGTCTCn'nnnn_
Cuts at: 0 213 578 1440
Size: 213 365 862
Fragments arranged by size:
862 365 213
Bsp1286I G_dGCh'C
Cuts at: 0 358 463 712 1440
Size: 358 105 249 728
Fragments arranged by size:
728 358 249 105
BspEI T'CCGG_A
Cuts at: 0 1345 1440
Size: 1345 95
BsrBI CCG'CTC
Cuts at: 0 190 443 1440
Size: 190 253 997
Fragments arranged by size:
997 253 190
BsrDI GCAATG_nn'
Cuts at: 0 954 1440
Size: 954 486
BsrFI r'CCGG_y
Cuts at: 0 759 819 931 1033 1440
Size: 759 60 112 102 407
Fragments arranged by size:
759 407 112 102 60
BssHII G'CGCG_C
Cuts at: 0 202 1440
Size: 202 1238
BstAPI GCAn_nnn'nTGC
Cuts at: 0 117 1440
Size: 117 1323
BstEII G'GTnAC_C
Cuts at: 0 1425 1440
Size: 1425 15
BstYI r'GATC_y
Cuts at: 0 1175 1440
Size: 1175 265
BtgI C'CryG_G
Cuts at: 0 560 590 1433 1440
Size: 560 30 843 7
Fragments arranged by size:
843 560 30 7
BtsI GCAGTG_nn'
Cuts at: 0 373 1440
Size: 373 1067
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 248 283 936 971 1098 1133 1440
Size: 248 35 653 35 127 35 307
Fragments arranged by size:
653 307 248 127 35 35 35
EaeI y'GGCC_r
Cuts at: 0 41 933 1019 1401 1440
Size: 41 892 86 382 39
Fragments arranged by size:
892 382 86 41 39
EagI C'GGCC_G
Cuts at: 0 41 1019 1440
Size: 41 978 421
Fragments arranged by size:
978 421 41
EarI CTCTTCn'nnn_
Cuts at: 0 796 1023 1210 1440
Size: 796 227 187 230
Fragments arranged by size:
796 230 227 187
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1246 1440
Size: 1246 194
EcoICRI GAG'CTC
Cuts at: 0 461 1440
Size: 461 979
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 413 422 560 810 1266 1320 1371
Size: 107 306 9 138 250 456 54 51
Cuts at: 1371 1440
Size: 69
Fragments arranged by size:
456 306 250 138 107 69 54 51
9
EcoO109I rG'GnC_Cy
Cuts at: 0 397 1098 1440
Size: 397 701 342
Fragments arranged by size:
701 397 342
FspI TGC'GCA
Cuts at: 0 56 1440
Size: 56 1384
HaeII r_GCGC'y
Cuts at: 0 13 1083 1440
Size: 13 1070 357
Fragments arranged by size:
1070 357 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 980 1012 1167 1199 1234 1258 1266 1290
Size: 980 32 155 32 35 24 8 24
Cuts at: 1290 1440
Size: 150
Fragments arranged by size:
980 155 150 35 32 32 24 24
8
HincII GTy'rAC
Cuts at: 0 888 1146 1440
Size: 888 258 294
Fragments arranged by size:
888 294 258
HpaI GTT'AAC
Cuts at: 0 1146 1440
Size: 1146 294
KasI G'GCGC_C
Cuts at: 0 9 1079 1440
Size: 9 1070 361
Fragments arranged by size:
1070 361 9
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1226 1390 1440
Size: 1226 164 50
Fragments arranged by size:
1226 164 50
MslI CAynn'nnrTG
Cuts at: 0 664 1440
Size: 664 776
MspA1I CmG'CkG
Cuts at: 0 114 322 388 455 592 785 1291 1440
Size: 114 208 66 67 137 193 506 149
Fragments arranged by size:
506 208 193 149 137 114 67 66
NaeI GCC'GGC
Cuts at: 0 761 1035 1440
Size: 761 274 405
Fragments arranged by size:
761 405 274
NarI GG'CG_CC
Cuts at: 0 10 1080 1440
Size: 10 1070 360
Fragments arranged by size:
1070 360 10
NcoI C'CATG_G
Cuts at: 0 1433 1440
Size: 1433 7
NgoMIV G'CCGG_C
Cuts at: 0 759 1033 1440
Size: 759 274 407
Fragments arranged by size:
759 407 274
NspI r_CATG'y
Cuts at: 0 1263 1440
Size: 1263 177
PasI CC'CwG_GG
Cuts at: 0 424 1440
Size: 424 1016
PflMI CCAn_nnn'nTGG
Cuts at: 0 430 1076 1440
Size: 430 646 364
Fragments arranged by size:
646 430 364
PpuMI rG'GwC_Cy
Cuts at: 0 1098 1440
Size: 1098 342
PvuII CAG'CTG
Cuts at: 0 114 388 1291 1440
Size: 114 274 903 149
Fragments arranged by size:
903 274 149 114
SacI G_AGCT'C
Cuts at: 0 463 1440
Size: 463 977
SacII CC_GC'GG
Cuts at: 0 593 1440
Size: 593 847
SanDI GG'GwC_CC
Cuts at: 0 1098 1440
Size: 1098 342
SapI GCTCTTCn'nnn_
Cuts at: 0 1023 1210 1440
Size: 1023 187 230
Fragments arranged by size:
1023 230 187
SexAI A'CCwGG_T
Cuts at: 0 638 1440
Size: 638 802
SfcI C'TryA_G
Cuts at: 0 213 482 777 1440
Size: 213 269 295 663
Fragments arranged by size:
663 295 269 213
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 404 1440
Size: 404 1036
SfoI GGC'GCC
Cuts at: 0 11 1081 1440
Size: 11 1070 359
Fragments arranged by size:
1070 359 11
SmaI CCC'GGG
Cuts at: 0 80 278 1440
Size: 80 198 1162
Fragments arranged by size:
1162 198 80
SphI G_CATG'C
Cuts at: 0 1263 1440
Size: 1263 177
StuI AGG'CCT
Cuts at: 0 695 1440
Size: 695 745
StyI C'CwwG_G
Cuts at: 0 1433 1440
Size: 1433 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 537 546 727 1440
Size: 537 9 181 713
Fragments arranged by size:
713 537 181 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 484 516 1369 1401 1440
Size: 484 32 853 32 39
Fragments arranged by size:
853 484 39 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 628 1102 1440
Size: 628 474 338
Fragments arranged by size:
628 474 338
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 973 1440
Size: 973 467
XmaI C'CCGG_G
Cuts at: 0 78 276 1440
Size: 78 198 1164
Fragments arranged by size:
1164 198 78
ZraI GAC'GTC
Cuts at: 0 138 1440
Size: 138 1302
Enzymes that do cut:
AatII AcuI AlfI AlwNI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BglI Bme1580I BplI BpmI Bpu10I
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BsrBI BsrDI BsrFI BssHII
BstAPI BstEII BstYI BtgI BtsI CspCI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I FspI HaeII Hin4I
HincII HpaI KasI MmeI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PflMI PpuMI PvuII SacI
SacII SanDI SapI SexAI SfcI SfiI SfoI SmaI
SphI StuI StyI TaqII TstI Tth111I XcmI XmaI
ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmrI BmtI BpuEI BsaI BsaAI BsaBI
BsiWI BspHI BspMI BsrGI BssSI BstBI BstXI BstZ17I
Bsu36I BtgZI ClaI DraI DraIII DrdI EcoNI EcoRI
EcoRV FalI FseI FspAI HindIII KpnI MfeI MluI
MscI NdeI NheI NotI NruI NsiI PacI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SalI SbfI ScaI SgfI
SgrAI SmlI SnaBI SpeI SrfI SspI SwaI TatI
XbaI XhoI XmnI