Restriction map for
pFN21AB7719
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AvrII
BamHI BanI BbeI BbrPI BbsI
BbvCI BciVI BclI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BseAI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraII DraIII
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HaeII HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MseI MunI NarI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tsp509I Van91I XbaI
XhoI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AflIII 848 ACATGT A^C[GA][CT]GT
Asp700I 270 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 694 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1119 CCCGGG C^[CT]CG[GA]G
AviII 1169 TGCGCA TGC^GCA
BalI 1001 TGGCCA TGG^CCA
BanII 931 GAGCCC G[GA]GC[CT]^C
BcgI 217 CGACGCCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1274 AGATCT A^GATCT
BsaWI 50 ACCGGA [AT]^CCGG[AT]
BsmBI 1014 CGTCTC CGTCTC(1/5)
BsoBI 1119 CCCGGG C^[CT]CG[GA]G
BspLU11I 848 ACATGT A^CATGT
BsrFI 236 GCCGGC [GA]^CCGG[CT]
BstAPI 987 GCATGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 398 CCATACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 236 GCCGGC [GA]^CCGG[CT]
DsaI 839 CCATGG C^C[GA][CT]GG
EarI 576 CTCTTC CTCTTC(1/4)
EcoNI 1176 CCTCAATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1169 TGCGCA TGC^GCA
Ksp632I 576 CTCTTC CTCTTC(1/4)
MluNI 1001 TGGCCA TGG^CCA
MscI 1001 TGGCCA TGG^CCA
NaeI 236 GCCGGC GCC^GGC
NcoI 839 CCATGG C^CATGG
NgoMIV 236 GCCGGC G^CCGGC
NheI 1158 GCTAGC G^CTAGC
NlaIV 491 GGAGCC GG[ACGT]^[ACGT]CC
PflFI 694 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 1087 CTGCAG CTGCA^G
SexAI 695 ACCTGGT A^CC[AT]GGT
SfcI 1087 CTGCAG C^T[GA][CT]AG
SmaI 1119 CCCGGG CCC^GGG
TaqI 1256 TCGA T^CGA
Tth111I 694 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1256 TCGA T^CGA
XcmI 714 CCAAAAGGTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1119 CCCGGG C^CCGGG
XmaI 1119 CCCGGG C^CCGGG
XmnI 270 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccII 886 CGCG CG^CG
1047 CGCG
AhdI 844 GACGACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1069 GACCGCCTGTC
AspEI 844 GACGACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1069 GACCGCCTGTC
AspHI 1186 GAGCAC G[AT]GC[AT]^C
1252 GTGCTC
BfaI 655 CTAG C^TAG
1159 CTAG
BsaI 83 GAGACC GGTCTC(1/5)
755 GAGACC
BsiHKAI 1186 GAGCAC G[AT]GC[AT]^C
1252 GTGCTC
BstUI 886 CGCG CG^CG
1047 CGCG
EaeI 14 CGGCCA [CT]^GGCC[GA]
1001 TGGCCA
Eam1105I 844 GACGACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1069 GACCGCCTGTC
EcoT14I 206 CCAAGG C^C[AT][AT]GG
839 CCATGG
FauI 1113 CCCGC CCCGC(4/6)
309 GCGGG
MaeI 655 CTAG C^TAG
1159 CTAG
MslI 103 CACTTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
258 CATCGGCATG
MspA1I 1101 CCGCTG C[AC]G^C[GT]G
1203 CCGCTG
MvnI 886 CGCG CG^CG
1047 CGCG
NspI 848 ACATGT [GA]CATG^[CT]
987 GCATGT
StuI 230 AGGCCT AGG^CCT
341 AGGCCT
StyI 206 CCAAGG C^C[AT][AT]GG
839 CCATGG
TfiI 631 GATTC G^A[AT]TC
749 GATTC
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# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
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AciI AcyI AfaI AluI AlwI
AvaII BbvI BcnI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MspI MvaI MwoI NciI NdeII
NlaIII PleI RsaI Sau3AI Sau96I
ScrFI SfaNI TseI Tsp45I TspRI
XhoII