(Circular) (Six-base) MAPSORT of: pFN21AB7719.seq Check: 4632 from: 1 to: 5986 pFN21AB7719 5986 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2189 3144 2189 Size: 955 5031 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3796 278 Size: 53 83 186 936 2260 2468 Fragments arranged by size: 2468 2260 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3329 1937 Size: 1392 4594 Acc65I G'GTAC_C Cuts at: 3307 3307 Size: 5986 AclI AA'CG_TT Cuts at: 1563 4113 4486 1563 Size: 2550 373 3063 Fragments arranged by size: 3063 2550 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2131 2348 2568 2813 2984 4049 5433 5701 Size: 499 217 220 245 171 1065 1384 268 Cuts at: 5701 1632 Size: 1917 Fragments arranged by size: 1917 1384 1065 499 268 245 220 217 171 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5789 AflIII A'CryG_T Cuts at: 1203 2853 4885 5862 1203 Size: 1650 2032 977 1327 Fragments arranged by size: 2032 1650 1327 977 AgeI A'CCGG_T Cuts at: 4817 4817 Size: 5986 AhdI GACnn_n'nnGTC Cuts at: 2854 3079 4715 2854 Size: 225 1636 4125 Fragments arranged by size: 4125 1636 225 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5986 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2048 3152 1184 Size: 864 1104 4018 Fragments arranged by size: 4018 1104 864 AlwNI CAG_nnn'CTG Cuts at: 3729 5301 3729 Size: 1572 4414 ApaLI G'TGCA_C Cuts at: 4043 5199 4043 Size: 1156 4830 ApoI r'AATT_y Cuts at: 1493 3295 3521 3673 5782 1493 Size: 1802 226 152 2109 1697 Fragments arranged by size: 2109 1802 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4540 160 Size: 872 3508 1606 Fragments arranged by size: 3508 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 AvaI C'yCGr_G Cuts at: 1870 1943 3124 3311 1870 Size: 73 1181 187 4545 Fragments arranged by size: 4545 1181 187 73 BamHI G'GATC_C Cuts at: 3316 3683 3316 Size: 367 5619 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3307 4762 618 Size: 325 217 627 1520 1455 1842 Fragments arranged by size: 1842 1520 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2940 3305 1391 Size: 1549 365 4072 Fragments arranged by size: 4072 1549 365 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5015 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2211 2245 4177 4211 1903 Size: 34 274 34 1932 34 3678 Fragments arranged by size: 3678 1932 274 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3878 5088 3878 Size: 1210 4776 BclI T'GATC_A Cuts at: 1548 1548 Size: 5986 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4597 136 Size: 107 122 71 631 605 337 2588 1525 Fragments arranged by size: 2588 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 3279 5981 3279 Size: 2702 3284 BlpI GC'TnA_GC Cuts at: 3403 3403 Size: 5986 Bme1580I G_kGCm'C Cuts at: 1790 2067 4047 5203 1790 Size: 277 1980 1156 2573 Fragments arranged by size: 2573 1980 1156 277 BmgBI CAC'GTC Cuts at: 1318 2902 1318 Size: 1584 4402 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3717 4675 442 Size: 416 53 83 399 24 2300 958 1753 Fragments arranged by size: 2300 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3167 1056 Size: 2111 3875 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3211 3211 Size: 5986 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2064 2454 2503 2945 4646 1239 Size: 825 390 49 442 1701 2579 Fragments arranged by size: 2579 1701 825 442 390 49 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4111 4976 5274 4111 Size: 865 298 4823 Fragments arranged by size: 4823 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2082 2754 4649 882 Size: 617 583 672 1895 2219 Fragments arranged by size: 2219 1895 672 617 583 BsaAI yAC'GTr Cuts at: 493 1204 3777 493 Size: 711 2573 2702 Fragments arranged by size: 2702 2573 711 BsaBI GATnn'nnATC Cuts at: 5980 5980 Size: 5986 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2224 2415 3048 3793 Size: 53 83 186 936 691 191 633 745 Cuts at: 3793 4175 4810 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 745 691 635 633 382 191 186 83 53 BsaWI w'CCGG_w Cuts at: 2055 4419 4817 5091 5238 5649 2055 Size: 2364 398 274 147 411 2392 Fragments arranged by size: 2392 2364 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2537 2567 1178 Size: 30 165 30 1134 30 4597 Fragments arranged by size: 4597 1134 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2354 2357 2396 2539 2542 2545 2999 3002 Size: 1168 3 39 143 3 3 454 3 Cuts at: 3002 3203 1186 Size: 201 3969 Fragments arranged by size: 3969 1168 454 201 143 39 3 3 3 3 BseYI C'CCAG_C Cuts at: 2531 2670 5189 5685 2531 Size: 139 2519 496 2832 Fragments arranged by size: 2832 2519 496 139 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2324 3062 3253 1163 Size: 573 588 738 191 3896 Fragments arranged by size: 3896 738 588 573 191 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3436 4197 5225 1540 Size: 275 186 1435 761 1028 2301 Fragments arranged by size: 2301 1435 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3195 3261 4047 4132 5203 3195 Size: 66 786 85 1071 3978 Fragments arranged by size: 3978 1071 786 85 66 BsmI GAATG_Cn' Cuts at: 3497 3590 3497 Size: 93 5893 BsmBI CGTCTCn'nnnn_ Cuts at: 3025 3025 Size: 5986 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 2940 3195 3261 3305 4047 4132 Size: 399 277 873 255 66 44 742 85 Cuts at: 4132 5203 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 873 742 399 277 255 85 66 44 BspEI T'CCGG_A Cuts at: 5649 5649 Size: 5986 BspHI T'CATG_A Cuts at: 3875 5605 3875 Size: 1730 4256 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2189 3144 3180 3341 844 Size: 1345 955 36 161 3489 Fragments arranged by size: 3489 1345 955 161 36 BsrBI CCG'CTC Cuts at: 1394 1823 3873 1394 Size: 429 2050 3507 Fragments arranged by size: 3507 2050 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2019 2397 3280 4481 4655 65 Size: 1183 771 378 883 1201 174 1396 Fragments arranged by size: 1396 1201 1183 883 771 378 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2241 4630 4817 1700 Size: 110 431 2389 187 2869 Fragments arranged by size: 2869 2389 431 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5986 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5986 BssSI C'ACGA_G Cuts at: 4046 5058 4046 Size: 1012 4974 BstAPI GCAn_nnn'nTGC Cuts at: 794 2998 794 Size: 2204 3782 BstBI TT'CG_AA Cuts at: 4790 4790 Size: 5986 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5986 BstXI CCAn_nnnn'nTGG Cuts at: 2410 2410 Size: 5986 BstYI r'GATC_y Cuts at: 1972 2070 2620 3279 3316 3683 4068 4085 5537 Size: 98 550 659 37 367 385 17 1452 Cuts at: 5537 5623 5635 5981 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 659 550 385 367 346 98 86 37 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2844 513 Size: 552 939 840 3655 Fragments arranged by size: 3655 939 840 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5247 Fragments arranged by size: 5247 683 56 BtsI GCAGTG_nn' Cuts at: 2227 3089 3502 4296 4316 2227 Size: 862 413 794 20 3897 Fragments arranged by size: 3897 862 794 413 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5986 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5951 DraI TTT'AAA Cuts at: 3290 3290 Size: 5986 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4993 809 Size: 507 3677 1802 Fragments arranged by size: 3677 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2019 3006 3433 Size: 54 1425 50 275 18 189 987 427 Cuts at: 3433 4322 5951 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 987 889 427 275 189 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3433 1537 Size: 275 1621 4090 Fragments arranged by size: 4090 1621 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2587 3916 1353 Size: 1234 1329 3423 Fragments arranged by size: 3423 1329 1234 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4573 4947 5093 387 Size: 1064 138 2984 374 146 1280 Fragments arranged by size: 2984 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2064 2131 2348 2454 2503 2568 2813 Size: 393 432 67 217 106 49 65 245 Cuts at: 2813 2945 2984 4049 4646 5433 5701 1239 Size: 132 39 1065 597 787 268 1524 Fragments arranged by size: 1524 1065 787 597 432 393 268 245 217 132 106 67 65 49 39 EcoNI CCTnn'n_nnAGG Cuts at: 3185 3185 Size: 5986 EcoRI G'AATT_C Cuts at: 3295 3673 3295 Size: 378 5608 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2992 2992 Size: 5986 FspI TGC'GCA Cuts at: 3176 4492 3176 Size: 1316 4670 HaeII r_GCGC'y Cuts at: 1678 5133 1678 Size: 3455 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2537 2569 2753 2785 2840 Size: 32 135 32 965 32 184 32 55 Cuts at: 2840 2872 3065 3097 4627 4659 4701 4733 1373 Size: 32 193 32 1530 32 42 32 2626 Fragments arranged by size: 2626 1530 965 193 184 135 55 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3330 3576 669 Size: 988 281 1392 246 3079 Fragments arranged by size: 3079 1392 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5986 HpaI GTT'AAC Cuts at: 3576 3576 Size: 5986 KpnI G_GTAC'C Cuts at: 3311 3311 Size: 5986 MfeI C'AATT_G Cuts at: 3585 3585 Size: 5986 MluI A'CGCG_T Cuts at: 5862 5862 Size: 5986 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2472 3108 5100 5284 1305 Size: 1167 636 1992 184 2007 Fragments arranged by size: 2007 1992 1167 636 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3008 10 Size: 54 1425 343 1176 2988 Fragments arranged by size: 2988 1425 1176 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2112 2267 3944 4303 4462 518 Size: 648 946 155 1677 359 159 2042 Fragments arranged by size: 2042 1677 946 648 359 159 155 MspA1I CmG'CkG Cuts at: 1606 3108 3210 3402 4079 5227 5472 1606 Size: 1502 102 192 677 1148 245 2120 Fragments arranged by size: 2120 1502 1148 677 245 192 102 NaeI GCC'GGC Cuts at: 2243 2243 Size: 5986 NcoI C'CATG_G Cuts at: 513 1065 2004 2844 513 Size: 552 939 840 3655 Fragments arranged by size: 3655 939 840 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5986 NgoMIV G'CCGG_C Cuts at: 2241 2241 Size: 5986 NheI G'CTAG_C Cuts at: 1052 3163 1052 Size: 2111 3875 NotI GC'GGCC_GC Cuts at: 3433 3433 Size: 5986 NspI r_CATG'y Cuts at: 1132 1229 2857 2996 3344 4889 1132 Size: 97 1628 139 348 1545 2229 Fragments arranged by size: 2229 1628 1545 348 139 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5986 PciI A'CATG_T Cuts at: 2853 4885 2853 Size: 2032 3954 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5986 PfoI T'CCnGG_A Cuts at: 2858 3069 2858 Size: 211 5775 PmeI GTTT'AAAC Cuts at: 3290 3290 Size: 5986 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4055 4087 5568 5600 4055 Size: 32 1481 32 4441 Fragments arranged by size: 4441 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5986 PsiI TTA'TAA Cuts at: 3556 3556 Size: 5986 PstI C_TGCA'G Cuts at: 830 3096 3338 830 Size: 2266 242 3478 Fragments arranged by size: 3478 2266 242 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5986 SalI G'TCGA_C Cuts at: 1936 3328 1936 Size: 1392 4594 SbfI CC_TGCA'GG Cuts at: 3338 3338 Size: 5986 ScaI AGT'ACT Cuts at: 4234 5946 4234 Size: 1712 4274 SexAI A'CCwGG_T Cuts at: 2700 2700 Size: 5986 SfcI C'TryA_G Cuts at: 826 1045 3092 3334 4469 5150 5341 826 Size: 219 2047 242 1135 681 191 1471 Fragments arranged by size: 2047 1471 1135 681 242 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5986 SmaI CCC'GGG Cuts at: 1872 3126 3313 1872 Size: 1254 187 4545 Fragments arranged by size: 4545 1254 187 SmlI C'TyrA_G Cuts at: 820 1017 1943 4090 4991 5253 820 Size: 197 926 2147 901 262 1553 Fragments arranged by size: 2147 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3777 493 Size: 3284 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5986 SphI G_CATG'C Cuts at: 1132 3344 1132 Size: 2212 3774 SspI AAT'ATT Cuts at: 5 52 3910 5 Size: 47 3858 2081 Fragments arranged by size: 3858 2081 47 StuI AGG'CCT Cuts at: 1517 2237 2348 1517 Size: 720 111 5155 Fragments arranged by size: 5155 720 111 StyI C'CwwG_G Cuts at: 513 1065 2004 2211 2844 3425 513 Size: 552 939 207 633 581 3074 Fragments arranged by size: 3074 939 633 581 552 207 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2106 4013 4030 4183 4368 4813 325 Size: 1255 526 1907 17 153 185 445 1498 Fragments arranged by size: 1907 1498 1255 526 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4232 5944 96 Size: 274 80 33 51 1443 2255 1712 138 Fragments arranged by size: 2255 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5946 5978 5946 Size: 32 5954 Tth111I GACn'n_nGTC Cuts at: 1316 2702 1316 Size: 1386 4600 XbaI T'CTAG_A Cuts at: 3322 3322 Size: 5986 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2726 2011 Size: 715 5271 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5986 XmaI C'CCGG_G Cuts at: 1870 3124 3311 1870 Size: 1254 187 4545 Fragments arranged by size: 4545 1254 187 XmnI GAAnn'nnTTC Cuts at: 2279 4115 2279 Size: 1836 4150 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3794 276 Size: 53 83 186 936 2260 2468 Fragments arranged by size: 2468 2260 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI