Restriction map for
pFN21AB7719
(Linear) (Six-base) MAPSORT of: KIBB7719.seq Check: 9503 from: 1 to: 1281
KIBB7719.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 184 1139 1281
Size: 184 955 142
Fragments arranged by size:
955 184 142
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 126 343 563 808 979 1281
Size: 126 217 220 245 171 302
Fragments arranged by size:
302 245 220 217 171 126
AflIII A'CryG_T
Cuts at: 0 848 1281
Size: 848 433
AhdI GACnn_n'nnGTC
Cuts at: 0 849 1074 1281
Size: 849 225 207
Fragments arranged by size:
849 225 207
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 43 1147 1281
Size: 43 1104 134
Fragments arranged by size:
1104 134 43
AvaI C'yCGr_G
Cuts at: 0 1119 1281
Size: 1119 162
BanII G_rGCy'C
Cuts at: 0 935 1281
Size: 935 346
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 206 240 1281
Size: 206 34 1041
Fragments arranged by size:
1041 206 34
BglII A'GATC_T
Cuts at: 0 1274 1281
Size: 1274 7
Bme1580I G_kGCm'C
Cuts at: 0 62 1281
Size: 62 1219
BmgBI CAC'GTC
Cuts at: 0 897 1281
Size: 897 384
BmtI G_CTAG'C
Cuts at: 0 1162 1281
Size: 1162 119
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1206 1281
Size: 1206 75
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 449 498 940 1281
Size: 59 390 49 442 341
Fragments arranged by size:
442 390 341 59 49
BsaI GGTCTCn'nnnn_
Cuts at: 0 77 749 1281
Size: 77 672 532
Fragments arranged by size:
672 532 77
BsaHI Gr'CG_yC
Cuts at: 0 219 410 1043 1281
Size: 219 191 633 238
Fragments arranged by size:
633 238 219 191
BsaWI w'CCGG_w
Cuts at: 0 50 1281
Size: 50 1231
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 532 562 1281
Size: 532 30 719
Fragments arranged by size:
719 532 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 349 352 391 534 537 540 994 997
Size: 349 3 39 143 3 3 454 3
Cuts at: 997 1198 1281
Size: 201 83
Fragments arranged by size:
454 349 201 143 83 39 3 3
3 3
BseYI C'CCAG_C
Cuts at: 0 526 665 1281
Size: 526 139 616
Fragments arranged by size:
616 526 139
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 1057 1248 1281
Size: 319 738 191 33
Fragments arranged by size:
738 319 191 33
BsiHKAI G_wGCw'C
Cuts at: 0 1190 1256 1281
Size: 1190 66 25
Fragments arranged by size:
1190 66 25
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1020 1281
Size: 1020 261
Bsp1286I G_dGCh'C
Cuts at: 0 62 935 1190 1256 1281
Size: 62 873 255 66 25
Fragments arranged by size:
873 255 66 62 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 184 1139 1175 1281
Size: 184 955 36 106
Fragments arranged by size:
955 184 106 36
BsrDI GCAATG_nn'
Cuts at: 0 14 392 1275 1281
Size: 14 378 883 6
Fragments arranged by size:
883 378 14 6
BsrFI r'CCGG_y
Cuts at: 0 236 1281
Size: 236 1045
BstAPI GCAn_nnn'nTGC
Cuts at: 0 993 1281
Size: 993 288
BstXI CCAn_nnnn'nTGG
Cuts at: 0 405 1281
Size: 405 876
BstYI r'GATC_y
Cuts at: 0 65 615 1274 1281
Size: 65 550 659 7
Fragments arranged by size:
659 550 65 7
BtgI C'CryG_G
Cuts at: 0 839 1281
Size: 839 442
BtsI GCAGTG_nn'
Cuts at: 0 222 1084 1281
Size: 222 862 197
Fragments arranged by size:
862 222 197
EaeI y'GGCC_r
Cuts at: 0 14 1001 1281
Size: 14 987 280
Fragments arranged by size:
987 280 14
EarI CTCTTCn'nnn_
Cuts at: 0 582 1281
Size: 582 699
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 126 343 449 498 563 808 940
Size: 59 67 217 106 49 65 245 132
Cuts at: 940 979 1281
Size: 39 302
Fragments arranged by size:
302 245 217 132 106 67 65 59
49 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1180 1281
Size: 1180 101
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 987 1281
Size: 987 294
FspI TGC'GCA
Cuts at: 0 1171 1281
Size: 1171 110
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 532 564 748 780 835 867 1060 1092
Size: 532 32 184 32 55 32 193 32
Cuts at: 1092 1281
Size: 189
Fragments arranged by size:
532 193 189 184 55 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 467 1103 1281
Size: 467 636 178
Fragments arranged by size:
636 467 178
MscI TGG'CCA
Cuts at: 0 1003 1281
Size: 1003 278
MslI CAynn'nnrTG
Cuts at: 0 107 262 1281
Size: 107 155 1019
Fragments arranged by size:
1019 155 107
MspA1I CmG'CkG
Cuts at: 0 1103 1205 1281
Size: 1103 102 76
Fragments arranged by size:
1103 102 76
NaeI GCC'GGC
Cuts at: 0 238 1281
Size: 238 1043
NcoI C'CATG_G
Cuts at: 0 839 1281
Size: 839 442
NgoMIV G'CCGG_C
Cuts at: 0 236 1281
Size: 236 1045
NheI G'CTAG_C
Cuts at: 0 1158 1281
Size: 1158 123
NspI r_CATG'y
Cuts at: 0 852 991 1281
Size: 852 139 290
Fragments arranged by size:
852 290 139
PciI A'CATG_T
Cuts at: 0 848 1281
Size: 848 433
PfoI T'CCnGG_A
Cuts at: 0 853 1064 1281
Size: 853 211 217
Fragments arranged by size:
853 217 211
PstI C_TGCA'G
Cuts at: 0 1091 1281
Size: 1091 190
SexAI A'CCwGG_T
Cuts at: 0 695 1281
Size: 695 586
SfcI C'TryA_G
Cuts at: 0 1087 1281
Size: 1087 194
SmaI CCC'GGG
Cuts at: 0 1121 1281
Size: 1121 160
StuI AGG'CCT
Cuts at: 0 232 343 1281
Size: 232 111 938
Fragments arranged by size:
938 232 111
StyI C'CwwG_G
Cuts at: 0 206 839 1281
Size: 206 633 442
Fragments arranged by size:
633 442 206
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 101 1281
Size: 101 1180
Tth111I GACn'n_nGTC
Cuts at: 0 697 1281
Size: 697 584
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 721 1281
Size: 721 560
XmaI C'CCGG_G
Cuts at: 0 1119 1281
Size: 1119 162
XmnI GAAnn'nnTTC
Cuts at: 0 274 1281
Size: 274 1007
Enzymes that do cut:
AarI AcuI AflIII AhdI AlfI AvaI BanII BcgI
BglII Bme1580I BmgBI BmtI BplI BpmI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BspMI BsrDI BsrFI BstAPI BstXI BstYI BtgI BtsI
EaeI EarI Eco57MI EcoNI FalI FspI Hin4I MmeI
MscI MslI MspA1I NaeI NcoI NgoMIV NheI NspI
PciI PfoI PstI SexAI SfcI SmaI StuI StyI
TaqII Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BciVI
BclI BfrBI BglI BlpI BmrI Bpu10I BpuEI BsaAI
BsaBI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrGI
BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoO109I
EcoRI EcoRV FseI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI NarI NdeI NotI NruI
NsiI PacI PasI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TatI TstI XbaI XhoI ZraI