(Circular) (Six-base) MAPSORT of: pFN21AB7710.seq Check: 514 from: 1 to: 6100 pFN21AB7710 6100 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2724 3268 2724 Size: 544 5556 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3910 278 Size: 53 83 186 936 2374 2468 Fragments arranged by size: 2468 2374 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2401 3443 1937 Size: 464 1042 4594 Fragments arranged by size: 4594 1042 464 Acc65I G'GTAC_C Cuts at: 3421 3421 Size: 6100 AclI AA'CG_TT Cuts at: 1563 4227 4600 1563 Size: 2664 373 3063 Fragments arranged by size: 3063 2664 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2045 4163 5547 5815 1632 Size: 413 2118 1384 268 1917 Fragments arranged by size: 2118 1917 1384 413 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5903 AflIII A'CryG_T Cuts at: 1203 4999 5976 1203 Size: 3796 977 1327 Fragments arranged by size: 3796 1327 977 AgeI A'CCGG_T Cuts at: 4931 4931 Size: 6100 AhdI GACnn_n'nnGTC Cuts at: 2944 4829 2944 Size: 1885 4215 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6100 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2183 3236 1184 Size: 999 1053 4048 Fragments arranged by size: 4048 1053 999 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3361 3393 3361 Size: 32 6068 AlwNI CAG_nnn'CTG Cuts at: 2542 3843 5415 2542 Size: 1301 1572 3227 Fragments arranged by size: 3227 1572 1301 ApaI G_GGCC'C Cuts at: 2149 2234 2149 Size: 85 6015 ApaLI G'TGCA_C Cuts at: 4157 5313 4157 Size: 1156 4944 ApoI r'AATT_y Cuts at: 1493 3409 3635 3787 5896 1493 Size: 1916 226 152 2109 1697 Fragments arranged by size: 2109 1916 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4654 160 Size: 872 3622 1606 Fragments arranged by size: 3622 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6100 AvaI C'yCGr_G Cuts at: 1870 1943 2155 3028 3208 3425 1870 Size: 73 212 873 180 217 4545 Fragments arranged by size: 4545 873 217 212 180 73 BamHI G'GATC_C Cuts at: 3430 3797 3430 Size: 367 5733 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3045 3212 3421 4876 618 Size: 325 217 627 1258 167 209 1455 1842 Fragments arranged by size: 1842 1455 1258 627 325 217 209 167 BanII G_rGCy'C Cuts at: 1391 2149 2234 2659 3312 3419 1391 Size: 758 85 425 653 107 4072 Fragments arranged by size: 4072 758 653 425 107 85 BbeI G_GCGC'C Cuts at: 3049 3049 Size: 6100 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2463 2753 2943 3340 928 Size: 971 564 290 190 397 3688 Fragments arranged by size: 3688 971 564 397 290 190 BbvCI CC'TCA_GC Cuts at: 2576 2576 Size: 6100 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2276 2310 4291 4325 1903 Size: 34 50 34 255 34 1981 34 3678 Fragments arranged by size: 3678 1981 255 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3992 5202 3992 Size: 1210 4890 BclI T'GATC_A Cuts at: 1548 3171 1548 Size: 1623 4477 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4711 136 Size: 107 122 71 631 605 3039 1525 Fragments arranged by size: 3039 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2271 6095 2271 Size: 3824 2276 BlpI GC'TnA_GC Cuts at: 3227 3517 3227 Size: 290 5810 Bme1580I G_kGCm'C Cuts at: 1790 2149 2226 2234 3215 4161 5317 1790 Size: 359 77 8 981 946 1156 2573 Fragments arranged by size: 2573 1156 981 946 359 77 8 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6100 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3831 4789 442 Size: 416 53 83 399 24 2414 958 1753 Fragments arranged by size: 2414 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6100 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2093 2564 2606 2693 4760 1239 Size: 854 471 42 87 2067 2579 Fragments arranged by size: 2579 2067 854 471 87 42 Bpu10I CC'TnA_GC Cuts at: 2576 2576 Size: 6100 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4225 5090 5388 4225 Size: 865 298 4937 Fragments arranged by size: 4937 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2635 4763 882 Size: 617 1136 2128 2219 Fragments arranged by size: 2219 2128 1136 617 BsaAI yAC'GTr Cuts at: 493 1204 3891 493 Size: 711 2687 2702 Fragments arranged by size: 2702 2687 711 BsaBI GATnn'nnATC Cuts at: 6094 6094 Size: 6100 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3046 3159 3907 4289 Size: 53 83 186 936 1513 113 748 382 Cuts at: 4289 4924 275 Size: 635 1451 Fragments arranged by size: 1513 1451 936 748 635 382 186 113 83 53 BsaWI w'CCGG_w Cuts at: 4533 4931 5205 5352 5763 4533 Size: 398 274 147 411 4870 Fragments arranged by size: 4870 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2646 2666 2676 2696 2808 Size: 30 165 30 1243 20 10 20 112 Cuts at: 2808 2838 1178 Size: 30 4440 Fragments arranged by size: 4440 1243 165 112 30 30 30 20 20 10 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2204 2207 2270 2690 3028 3136 1186 Size: 1018 3 63 420 338 108 4150 Fragments arranged by size: 4150 1018 420 338 108 63 3 BseYI C'CCAG_C Cuts at: 2236 3073 3192 5303 5799 2236 Size: 837 119 2111 496 2537 Fragments arranged by size: 2537 2111 837 496 119 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3076 1163 Size: 573 1340 4187 Fragments arranged by size: 4187 1340 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3550 4311 5339 1540 Size: 275 186 1549 761 1028 2301 Fragments arranged by size: 2301 1549 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2262 2420 4161 4246 5317 2262 Size: 158 1741 85 1071 3045 Fragments arranged by size: 3045 1741 1071 158 85 BsmI GAATG_Cn' Cuts at: 3611 3704 3611 Size: 93 6007 BsmBI CGTCTCn'nnnn_ Cuts at: 2396 2396 Size: 6100 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2149 2226 2234 2262 2420 2659 3215 Size: 399 359 77 8 28 158 239 556 Cuts at: 3215 3312 3419 4161 4246 5317 1391 Size: 97 107 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 556 399 359 239 158 107 97 85 77 28 8 BspEI T'CCGG_A Cuts at: 5763 5763 Size: 6100 BspHI T'CATG_A Cuts at: 3989 5719 3989 Size: 1730 4370 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2724 3049 3075 3228 3268 3455 844 Size: 1880 325 26 153 40 187 3489 Fragments arranged by size: 3489 1880 325 187 153 40 26 BsrBI CCG'CTC Cuts at: 1394 1823 2179 3987 1394 Size: 429 356 1808 3507 Fragments arranged by size: 3507 1808 429 356 BsrDI GCAATG_nn' Cuts at: 65 1248 4595 4769 65 Size: 1183 3347 174 1396 Fragments arranged by size: 3347 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2112 4744 4931 1700 Size: 110 302 2632 187 2869 Fragments arranged by size: 2869 2632 302 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6100 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6100 BssSI C'ACGA_G Cuts at: 4160 5172 4160 Size: 1012 5088 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6100 BstBI TT'CG_AA Cuts at: 4904 4904 Size: 6100 BstEII G'GTnAC_C Cuts at: 1184 2083 3280 1184 Size: 899 1197 4004 Fragments arranged by size: 4004 1197 899 BstYI r'GATC_y Cuts at: 1972 2163 2271 2793 3234 3430 3797 4182 4199 Size: 191 108 522 441 196 367 385 17 Cuts at: 4199 5651 5737 5749 6095 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 522 441 385 367 346 196 191 108 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2763 2763 Size: 6100 BtgI C'CryG_G Cuts at: 513 1065 2211 2349 513 Size: 552 1146 138 4264 Fragments arranged by size: 4264 1146 552 138 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2569 487 Size: 683 56 1343 4018 Fragments arranged by size: 4018 1343 683 56 BtsI GCAGTG_nn' Cuts at: 3194 3324 3616 4410 4430 3194 Size: 130 292 794 20 4864 Fragments arranged by size: 4864 794 292 130 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6100 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6065 DraI TTT'AAA Cuts at: 3404 3404 Size: 6100 DraIII CAC_nnn'GTG Cuts at: 2350 2350 Size: 6100 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5107 809 Size: 507 3791 1802 Fragments arranged by size: 3791 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2568 3105 3547 Size: 54 1425 50 275 18 738 537 442 Cuts at: 3547 4436 6065 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 738 537 442 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3547 1537 Size: 275 1735 4090 Fragments arranged by size: 4090 1735 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2966 4030 1353 Size: 1613 1064 3423 Fragments arranged by size: 3423 1613 1064 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4687 5061 5207 387 Size: 1064 138 3098 374 146 1280 Fragments arranged by size: 3098 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2045 2093 2564 2606 2693 4163 4760 Size: 393 413 48 471 42 87 1470 597 Cuts at: 4760 5547 5815 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1470 787 597 471 413 393 268 87 48 42 EcoNI CCTnn'n_nnAGG Cuts at: 2363 2363 Size: 6100 EcoO109I rG'GnC_Cy Cuts at: 2145 2327 2145 Size: 182 5918 EcoRI G'AATT_C Cuts at: 3409 3787 3409 Size: 378 5722 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2293 2293 Size: 6100 FspI TGC'GCA Cuts at: 4606 4606 Size: 6100 HaeII r_GCGC'y Cuts at: 1678 3049 3150 5247 1678 Size: 1371 101 2097 2531 Fragments arranged by size: 2531 2097 1371 101 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2646 2678 2930 2962 4741 Size: 32 135 32 1074 32 252 32 1779 Cuts at: 4741 4773 4815 4847 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1779 1074 252 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2402 2725 3064 3444 3690 669 Size: 988 281 464 323 339 380 246 3079 Fragments arranged by size: 3079 988 464 380 339 323 281 246 HindIII A'AGCT_T Cuts at: 748 2465 748 Size: 1717 4383 HpaI GTT'AAC Cuts at: 3690 3690 Size: 6100 KasI G'GCGC_C Cuts at: 3045 3045 Size: 6100 KpnI G_GTAC'C Cuts at: 3425 3425 Size: 6100 MfeI C'AATT_G Cuts at: 3699 3699 Size: 6100 MluI A'CGCG_T Cuts at: 5976 5976 Size: 6100 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5214 5398 1305 Size: 3909 184 2007 Fragments arranged by size: 3909 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2570 10 Size: 54 1425 343 738 3540 Fragments arranged by size: 3540 1425 738 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4058 4417 4576 518 Size: 648 2892 359 159 2042 Fragments arranged by size: 2892 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2071 2236 3054 3073 3226 3516 4193 5341 Size: 465 165 818 19 153 290 677 1148 Cuts at: 5341 5586 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 818 677 465 290 245 165 153 19 NaeI GCC'GGC Cuts at: 2114 2114 Size: 6100 NarI GG'CG_CC Cuts at: 3046 3046 Size: 6100 NcoI C'CATG_G Cuts at: 513 1065 2211 513 Size: 552 1146 4402 Fragments arranged by size: 4402 1146 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6100 NgoMIV G'CCGG_C Cuts at: 2112 2112 Size: 6100 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6100 NotI GC'GGCC_GC Cuts at: 3547 3547 Size: 6100 NspI r_CATG'y Cuts at: 1132 1229 3458 5003 1132 Size: 97 2229 1545 2229 Fragments arranged by size: 2229 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2227 1348 Size: 879 5221 PciI A'CATG_T Cuts at: 4999 4999 Size: 6100 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6100 PfoI T'CCnGG_A Cuts at: 2358 2358 Size: 6100 PmeI GTTT'AAAC Cuts at: 3404 3404 Size: 6100 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4169 4201 5682 5714 4169 Size: 32 1481 32 4555 Fragments arranged by size: 4555 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2327 2327 Size: 6100 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6100 PsiI TTA'TAA Cuts at: 3670 3670 Size: 6100 PspOMI G'GGCC_C Cuts at: 2145 2230 2145 Size: 85 6015 PstI C_TGCA'G Cuts at: 830 2321 2535 3072 3452 830 Size: 1491 214 537 380 3478 Fragments arranged by size: 3478 1491 537 380 214 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6100 PvuII CAG'CTG Cuts at: 3073 3073 Size: 6100 SalI G'TCGA_C Cuts at: 1936 2400 3442 1936 Size: 464 1042 4594 Fragments arranged by size: 4594 1042 464 SanDI GG'GwC_CC Cuts at: 2327 2327 Size: 6100 SapI GCTCTTCn'nnn_ Cuts at: 2966 2966 Size: 6100 SbfI CC_TGCA'GG Cuts at: 3452 3452 Size: 6100 ScaI AGT'ACT Cuts at: 4348 6060 4348 Size: 1712 4388 SfcI C'TryA_G Cuts at: 826 1045 2317 2531 3068 3448 4583 5264 5455 Size: 219 1272 214 537 380 1135 681 191 Cuts at: 5455 826 Size: 1471 Fragments arranged by size: 1471 1272 1135 681 537 380 219 214 191 SfoI GGC'GCC Cuts at: 3047 3047 Size: 6100 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6100 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6100 SmaI CCC'GGG Cuts at: 1872 3030 3210 3427 1872 Size: 1158 180 217 4545 Fragments arranged by size: 4545 1158 217 180 SmlI C'TyrA_G Cuts at: 820 1017 1943 4204 5105 5367 820 Size: 197 926 2261 901 262 1553 Fragments arranged by size: 2261 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3891 493 Size: 3398 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6100 SphI G_CATG'C Cuts at: 1132 3458 1132 Size: 2326 3774 SspI AAT'ATT Cuts at: 5 52 4024 5 Size: 47 3972 2081 Fragments arranged by size: 3972 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6100 StyI C'CwwG_G Cuts at: 513 1065 2211 3539 513 Size: 552 1146 1328 3074 Fragments arranged by size: 3074 1328 1146 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4127 4144 4297 4482 4927 325 Size: 1255 2547 17 153 185 445 1498 Fragments arranged by size: 2547 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4346 6058 96 Size: 274 80 33 51 1443 2369 1712 138 Fragments arranged by size: 2369 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2432 2464 2666 2698 6060 6092 2432 Size: 32 202 32 3362 32 2440 Fragments arranged by size: 3362 2440 202 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2783 3158 1316 Size: 1467 375 4258 Fragments arranged by size: 4258 1467 375 XbaI T'CTAG_A Cuts at: 3436 3436 Size: 6100 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2920 2920 Size: 6100 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6100 XmaI C'CCGG_G Cuts at: 1870 3028 3208 3425 1870 Size: 1158 180 217 4545 Fragments arranged by size: 4545 1158 217 180 XmnI GAAnn'nnTTC Cuts at: 4229 4229 Size: 6100 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3908 276 Size: 53 83 186 936 2374 2468 Fragments arranged by size: 2468 2374 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SexAI SfiI SrfI SwaI