(Circular) (Six-base) MAPSORT of: pFN21AB7658.seq Check: 8973 from: 1 to: 5887 pFN21AB7658 5887 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3697 278 Size: 53 83 186 936 2161 2468 Fragments arranged by size: 2468 2161 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2706 3230 1937 Size: 769 524 4594 Fragments arranged by size: 4594 769 524 Acc65I G'GTAC_C Cuts at: 3208 3208 Size: 5887 AclI AA'CG_TT Cuts at: 1563 4014 4387 1563 Size: 2451 373 3063 Fragments arranged by size: 3063 2451 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2228 2508 2529 3950 5334 5602 1632 Size: 596 280 21 1421 1384 268 1917 Fragments arranged by size: 1917 1421 1384 596 280 268 21 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5690 AflIII A'CryG_T Cuts at: 1203 4786 5763 1203 Size: 3583 977 1327 Fragments arranged by size: 3583 1327 977 AgeI A'CCGG_T Cuts at: 4718 4718 Size: 5887 AhdI GACnn_n'nnGTC Cuts at: 4616 4616 Size: 5887 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5887 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5887 AlwNI CAG_nnn'CTG Cuts at: 3630 5202 3630 Size: 1572 4315 ApaI G_GGCC'C Cuts at: 2121 2121 Size: 5887 ApaLI G'TGCA_C Cuts at: 3944 5100 3944 Size: 1156 4731 ApoI r'AATT_y Cuts at: 1493 2901 2959 3196 3422 3574 5683 1493 Size: 1408 58 237 226 152 2109 1697 Fragments arranged by size: 2109 1697 1408 237 226 152 58 AseI AT'TA_AT Cuts at: 160 1032 4441 160 Size: 872 3409 1606 Fragments arranged by size: 3409 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5887 AvaI C'yCGr_G Cuts at: 1870 1943 3212 1870 Size: 73 1269 4545 Fragments arranged by size: 4545 1269 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2746 2779 2746 Size: 33 5854 BamHI G'GATC_C Cuts at: 2404 3217 3584 2404 Size: 813 367 4707 Fragments arranged by size: 4707 813 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3208 4663 618 Size: 325 217 627 1421 1455 1842 Fragments arranged by size: 1842 1455 1421 627 325 217 BanII G_rGCy'C Cuts at: 1391 2121 2237 3206 1391 Size: 730 116 969 4072 Fragments arranged by size: 4072 969 730 116 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2535 928 Size: 971 636 4280 Fragments arranged by size: 4280 971 636 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4078 4112 1903 Size: 34 2141 34 3678 Fragments arranged by size: 3678 2141 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3779 4989 3779 Size: 1210 4677 BclI T'GATC_A Cuts at: 1548 1548 Size: 5887 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4498 136 Size: 107 122 71 631 605 337 2489 1525 Fragments arranged by size: 2489 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5882 5882 Size: 5887 BlpI GC'TnA_GC Cuts at: 3304 3304 Size: 5887 Bme1580I G_kGCm'C Cuts at: 1790 2121 3948 5104 1790 Size: 331 1827 1156 2573 Fragments arranged by size: 2573 1827 1156 331 BmgBI CAC'GTC Cuts at: 1318 2424 1318 Size: 1106 4781 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2184 3618 4576 Size: 416 53 83 399 24 767 1434 958 Cuts at: 4576 442 Size: 1753 Fragments arranged by size: 1753 1434 958 767 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5887 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2710 2710 Size: 5887 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2165 2847 4547 1239 Size: 926 682 1700 2579 Fragments arranged by size: 2579 1700 926 682 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2221 4012 4877 5175 2221 Size: 1791 865 298 2933 Fragments arranged by size: 2933 1791 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2201 2581 3079 4550 882 Size: 617 702 380 498 1471 2219 Fragments arranged by size: 2219 1471 702 617 498 380 BsaAI yAC'GTr Cuts at: 493 1204 3678 493 Size: 711 2474 2702 Fragments arranged by size: 2702 2474 711 BsaBI GATnn'nnATC Cuts at: 5881 5881 Size: 5887 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3694 4076 4711 275 Size: 53 83 186 936 2161 382 635 1451 Fragments arranged by size: 2161 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4320 4718 4992 5139 5550 4320 Size: 398 274 147 411 4657 Fragments arranged by size: 4657 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5662 Fragments arranged by size: 5662 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2357 2670 3062 1186 Size: 1171 313 392 4011 Fragments arranged by size: 4011 1171 392 313 BseYI C'CCAG_C Cuts at: 2083 5090 5586 2083 Size: 3007 496 2384 Fragments arranged by size: 3007 2384 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5314 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3337 4098 5126 1540 Size: 275 186 1336 761 1028 2301 Fragments arranged by size: 2301 1336 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2151 2237 3948 4033 5104 2151 Size: 86 1711 85 1071 2934 Fragments arranged by size: 2934 1711 1071 86 85 BsmI GAATG_Cn' Cuts at: 3398 3491 3398 Size: 93 5794 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2121 2151 2237 3206 3948 4033 5104 Size: 399 331 30 86 969 742 85 1071 Cuts at: 5104 1391 Size: 2174 Fragments arranged by size: 2174 1071 969 742 399 331 86 85 30 BspEI T'CCGG_A Cuts at: 5550 5550 Size: 5887 BspHI T'CATG_A Cuts at: 2977 3073 3776 5506 2977 Size: 96 703 1730 3358 Fragments arranged by size: 3358 1730 703 96 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3242 844 Size: 2398 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3774 1394 Size: 429 1951 3507 Fragments arranged by size: 3507 1951 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4382 4556 65 Size: 1183 3134 174 1396 Fragments arranged by size: 3134 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4531 4718 1700 Size: 110 2721 187 2869 Fragments arranged by size: 2869 2721 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5887 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5887 BssSI C'ACGA_G Cuts at: 3947 4959 3947 Size: 1012 4875 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5887 BstBI TT'CG_AA Cuts at: 4691 4691 Size: 5887 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5887 BstYI r'GATC_y Cuts at: 1972 2404 2497 3217 3584 3969 3986 5438 5524 Size: 432 93 720 367 385 17 1452 86 Cuts at: 5524 5536 5882 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 720 432 385 367 346 93 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2340 2340 Size: 5887 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5335 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5148 Fragments arranged by size: 5148 683 56 BtsI GCAGTG_nn' Cuts at: 2377 2452 3403 4197 4217 2377 Size: 75 951 794 20 4047 Fragments arranged by size: 4047 951 794 75 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5887 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3088 3123 529 Size: 35 2524 35 3293 Fragments arranged by size: 3293 2524 35 35 DraI TTT'AAA Cuts at: 3191 3191 Size: 5887 DraIII CAC_nnn'GTG Cuts at: 2771 2771 Size: 5887 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2447 4894 809 Size: 507 1131 2447 1802 Fragments arranged by size: 2447 1802 1131 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3334 4223 5852 Size: 54 1425 50 275 18 1504 889 1629 Cuts at: 5852 8 Size: 43 Fragments arranged by size: 1629 1504 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3334 1537 Size: 275 1522 4090 Fragments arranged by size: 4090 1522 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3817 1353 Size: 2464 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2058 4474 4848 4994 387 Size: 1064 138 469 2416 374 146 1280 Fragments arranged by size: 2416 1280 1064 469 374 146 138 EcoICRI GAG'CTC Cuts at: 2235 2235 Size: 5887 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2165 2228 2508 2529 2847 3950 4547 Size: 393 533 63 280 21 318 1103 597 Cuts at: 4547 5334 5602 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1103 787 597 533 393 318 280 268 63 21 EcoO109I rG'GnC_Cy Cuts at: 2817 2817 Size: 5887 EcoRI G'AATT_C Cuts at: 2901 2959 3196 3574 2901 Size: 58 237 378 5214 Fragments arranged by size: 5214 378 237 58 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2068 2114 2227 2068 Size: 46 113 5728 Fragments arranged by size: 5728 113 46 FspI TGC'GCA Cuts at: 4393 4393 Size: 5887 HaeII r_GCGC'y Cuts at: 1678 5034 1678 Size: 3356 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2219 2251 2300 2302 2332 Size: 32 135 32 647 32 49 2 30 Cuts at: 2332 2334 2389 2421 4528 4560 4602 4634 1373 Size: 2 55 32 2107 32 42 32 2626 Fragments arranged by size: 2626 2107 647 135 55 49 42 32 32 32 32 32 32 30 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 3231 3477 669 Size: 988 281 1293 246 3079 Fragments arranged by size: 3079 1293 988 281 246 HindIII A'AGCT_T Cuts at: 748 2276 2863 748 Size: 1528 587 3772 Fragments arranged by size: 3772 1528 587 HpaI GTT'AAC Cuts at: 3477 3477 Size: 5887 KpnI G_GTAC'C Cuts at: 3212 3212 Size: 5887 MfeI C'AATT_G Cuts at: 3486 3486 Size: 5887 MluI A'CGCG_T Cuts at: 5763 5763 Size: 5887 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5001 5185 1305 Size: 3696 184 2007 Fragments arranged by size: 3696 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4065 Fragments arranged by size: 4065 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3845 4204 4363 518 Size: 648 2679 359 159 2042 Fragments arranged by size: 2679 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3303 3980 5128 5373 1606 Size: 1697 677 1148 245 2120 Fragments arranged by size: 2120 1697 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5335 NdeI CA'TA_TG Cuts at: 387 3091 387 Size: 2704 3183 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5887 NotI GC'GGCC_GC Cuts at: 3334 3334 Size: 5887 NspI r_CATG'y Cuts at: 1132 1229 3245 4790 1132 Size: 97 2016 1545 2229 Fragments arranged by size: 2229 2016 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5887 PciI A'CATG_T Cuts at: 4786 4786 Size: 5887 PflMI CCAn_nnn'nTGG Cuts at: 1108 2771 3023 1108 Size: 1663 252 3972 Fragments arranged by size: 3972 1663 252 PmeI GTTT'AAAC Cuts at: 3191 3191 Size: 5887 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3956 3988 5469 5501 3956 Size: 32 1481 32 4342 Fragments arranged by size: 4342 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2817 2817 Size: 5887 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5887 PsiI TTA'TAA Cuts at: 3457 3457 Size: 5887 PspOMI G'GGCC_C Cuts at: 2117 2117 Size: 5887 PstI C_TGCA'G Cuts at: 830 3069 3239 830 Size: 2239 170 3478 Fragments arranged by size: 3478 2239 170 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5887 SacI G_AGCT'C Cuts at: 2237 2237 Size: 5887 SalI G'TCGA_C Cuts at: 1936 3229 1936 Size: 1293 4594 SbfI CC_TGCA'GG Cuts at: 3239 3239 Size: 5887 ScaI AGT'ACT Cuts at: 4135 5847 4135 Size: 1712 4175 SfcI C'TryA_G Cuts at: 826 1045 2514 3065 3235 4370 5051 5242 826 Size: 219 1469 551 170 1135 681 191 1471 Fragments arranged by size: 1471 1469 1135 681 551 219 191 170 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5887 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5887 SmaI CCC'GGG Cuts at: 1872 3214 1872 Size: 1342 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2236 3991 4892 5154 820 Size: 197 926 293 1755 901 262 1553 Fragments arranged by size: 1755 1553 926 901 293 262 197 SnaBI TAC'GTA Cuts at: 493 3678 493 Size: 3185 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5887 SphI G_CATG'C Cuts at: 1132 3245 1132 Size: 2113 3774 SspI AAT'ATT Cuts at: 5 52 3811 5 Size: 47 3759 2081 Fragments arranged by size: 3759 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5887 StyI C'CwwG_G Cuts at: 513 1065 2211 2268 2571 3326 513 Size: 552 1146 57 303 755 3074 Fragments arranged by size: 3074 1146 755 552 303 57 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3914 3931 4084 4269 4714 325 Size: 1255 2334 17 153 185 445 1498 Fragments arranged by size: 2334 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4133 5845 96 Size: 274 80 33 51 1443 2156 1712 138 Fragments arranged by size: 2156 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5847 5879 5847 Size: 32 5855 Tth111I GACn'n_nGTC Cuts at: 1316 2314 1316 Size: 998 4889 XbaI T'CTAG_A Cuts at: 2683 3223 2683 Size: 540 5347 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2176 2176 Size: 5887 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5887 XmaI C'CCGG_G Cuts at: 1870 3212 1870 Size: 1342 4545 XmnI GAAnn'nnTTC Cuts at: 2864 3032 4016 2864 Size: 168 984 4735 Fragments arranged by size: 4735 984 168 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3695 276 Size: 53 83 186 936 2161 2468 Fragments arranged by size: 2468 2161 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI