(Circular) (Six-base) MAPSORT of: pFN21AB7595.seq Check: 3547 from: 1 to: 5404 pFN21AB7595 5404 bp With 182 enzymes: SgfI * February 27, 2009 14:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3214 278 Size: 53 83 186 936 1678 2468 Fragments arranged by size: 2468 1678 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2682 2747 1937 Size: 745 65 4594 Fragments arranged by size: 4594 745 65 Acc65I G'GTAC_C Cuts at: 2725 2725 Size: 5404 AclI AA'CG_TT Cuts at: 1563 3531 3904 1563 Size: 1968 373 3063 Fragments arranged by size: 3063 1968 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3467 4851 5119 1632 Size: 1835 1384 268 1917 Fragments arranged by size: 1917 1835 1384 268 AfeI AGC'GCT Cuts at: 2632 2632 Size: 5404 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5207 AflIII A'CryG_T Cuts at: 1203 4303 5280 1203 Size: 3100 977 1327 Fragments arranged by size: 3100 1327 977 AgeI A'CCGG_T Cuts at: 4235 4235 Size: 5404 AhdI GACnn_n'nnGTC Cuts at: 4133 4133 Size: 5404 AleI CACnn'nnGTG Cuts at: 1166 2450 1166 Size: 1284 4120 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5404 AlwNI CAG_nnn'CTG Cuts at: 2317 3147 4719 2317 Size: 830 1572 3002 Fragments arranged by size: 3002 1572 830 ApaI G_GGCC'C Cuts at: 2076 2076 Size: 5404 ApaLI G'TGCA_C Cuts at: 3461 4617 3461 Size: 1156 4248 ApoI r'AATT_y Cuts at: 1493 2713 2939 3091 5200 1493 Size: 1220 226 152 2109 1697 Fragments arranged by size: 2109 1697 1220 226 152 AseI AT'TA_AT Cuts at: 160 1032 3958 160 Size: 872 2926 1606 Fragments arranged by size: 2926 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5404 AvaI C'yCGr_G Cuts at: 1870 1943 2254 2729 1870 Size: 73 311 475 4545 Fragments arranged by size: 4545 475 311 73 BamHI G'GATC_C Cuts at: 2734 3101 2734 Size: 367 5037 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2132 2380 2725 4180 618 Size: 325 217 627 345 248 345 1455 1842 Fragments arranged by size: 1842 1455 627 345 345 325 248 217 BanII G_rGCy'C Cuts at: 1391 2076 2084 2531 2583 2723 1391 Size: 685 8 447 52 140 4072 Fragments arranged by size: 4072 685 447 140 52 8 BbeI G_GCGC'C Cuts at: 2136 2136 Size: 5404 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2069 2593 928 Size: 971 170 524 3739 Fragments arranged by size: 3739 971 524 170 BbvCI CC'TCA_GC Cuts at: 2102 2298 2559 2102 Size: 196 261 4947 Fragments arranged by size: 4947 261 196 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3595 3629 1903 Size: 34 1658 34 3678 Fragments arranged by size: 3678 1658 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3296 4506 3296 Size: 1210 4194 BclI T'GATC_A Cuts at: 1548 1548 Size: 5404 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2048 2342 4015 Size: 107 122 71 631 605 376 294 1673 Cuts at: 4015 136 Size: 1525 Fragments arranged by size: 1673 1525 631 605 376 294 122 107 71 BglII A'GATC_T Cuts at: 5399 5399 Size: 5404 BlpI GC'TnA_GC Cuts at: 2821 2821 Size: 5404 Bme1580I G_kGCm'C Cuts at: 1790 2076 3465 4621 1790 Size: 286 1389 1156 2573 Fragments arranged by size: 2573 1389 1156 286 BmgBI CAC'GTC Cuts at: 1318 2032 1318 Size: 714 4690 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3135 4093 442 Size: 416 53 83 399 24 1718 958 1753 Fragments arranged by size: 1753 1718 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5404 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2068 2294 2630 4064 1239 Size: 829 226 336 1434 2579 Fragments arranged by size: 2579 1434 829 336 226 Bpu10I CC'TnA_GC Cuts at: 2102 2298 2559 2102 Size: 196 261 4947 Fragments arranged by size: 4947 261 196 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2277 3529 4394 4692 2277 Size: 1252 865 298 2989 Fragments arranged by size: 2989 1252 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2537 4067 882 Size: 617 1038 1530 2219 Fragments arranged by size: 2219 1530 1038 617 BsaAI yAC'GTr Cuts at: 493 1204 3195 493 Size: 711 1991 2702 Fragments arranged by size: 2702 1991 711 BsaBI GATnn'nnATC Cuts at: 5398 5398 Size: 5404 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2133 3211 3593 4228 Size: 53 83 186 936 600 1078 382 635 Cuts at: 4228 275 Size: 1451 Fragments arranged by size: 1451 1078 936 635 600 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3837 4235 4509 4656 5067 3837 Size: 398 274 147 411 4174 Fragments arranged by size: 4174 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5179 Fragments arranged by size: 5179 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2060 2447 2501 1186 Size: 874 387 54 4089 Fragments arranged by size: 4089 874 387 54 BseYI C'CCAG_C Cuts at: 4607 5103 4607 Size: 496 4908 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2141 1163 Size: 573 405 4426 Fragments arranged by size: 4426 573 405 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2854 3615 4643 1540 Size: 275 186 853 761 1028 2301 Fragments arranged by size: 2301 1028 853 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2012 2457 3465 3550 4621 2012 Size: 445 1008 85 1071 2795 Fragments arranged by size: 2795 1071 1008 445 85 BsmI GAATG_Cn' Cuts at: 2915 3008 2915 Size: 93 5311 BsmBI CGTCTCn'nnnn_ Cuts at: 2491 2491 Size: 5404 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2076 2084 2457 2531 2583 2723 Size: 399 222 64 8 373 74 52 140 Cuts at: 2723 3465 3550 4621 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 373 222 140 85 74 64 52 8 BspEI T'CCGG_A Cuts at: 5067 5067 Size: 5404 BspHI T'CATG_A Cuts at: 3293 5023 3293 Size: 1730 3674 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2668 2759 844 Size: 1824 91 3489 Fragments arranged by size: 3489 1824 91 BsrBI CCG'CTC Cuts at: 1394 1823 3291 1394 Size: 429 1468 3507 Fragments arranged by size: 3507 1468 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2694 3899 4073 65 Size: 1183 1446 1205 174 1396 Fragments arranged by size: 1446 1396 1205 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4048 4235 1700 Size: 110 2238 187 2869 Fragments arranged by size: 2869 2238 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5404 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5404 BssSI C'ACGA_G Cuts at: 3464 4476 3464 Size: 1012 4392 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5404 BstBI TT'CG_AA Cuts at: 4208 4208 Size: 5404 BstEII G'GTnAC_C Cuts at: 1184 2618 1184 Size: 1434 3970 BstXI CCAn_nnnn'nTGG Cuts at: 2353 2590 2353 Size: 237 5167 BstYI r'GATC_y Cuts at: 1972 2734 3101 3486 3503 4955 5041 5053 5399 Size: 762 367 385 17 1452 86 12 346 Cuts at: 5399 1972 Size: 1977 Fragments arranged by size: 1977 1452 762 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4852 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2464 487 Size: 683 56 1238 3427 Fragments arranged by size: 3427 1238 683 56 BtsI GCAGTG_nn' Cuts at: 2920 3714 3734 2920 Size: 794 20 4590 Fragments arranged by size: 4590 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5404 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5369 DraI TTT'AAA Cuts at: 2708 2708 Size: 5404 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4411 809 Size: 507 3095 1802 Fragments arranged by size: 3095 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 2343 2851 Size: 54 1425 50 275 18 210 303 508 Cuts at: 2851 3740 5369 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 508 303 275 210 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2851 1537 Size: 275 1039 4090 Fragments arranged by size: 4090 1039 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2114 2150 2181 2426 3334 1353 Size: 761 36 31 245 908 3423 Fragments arranged by size: 3423 908 761 245 36 31 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3991 4365 4511 387 Size: 1064 138 2402 374 146 1280 Fragments arranged by size: 2402 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2068 2294 2630 3467 4064 4851 5119 Size: 393 436 226 336 837 597 787 268 Cuts at: 5119 1239 Size: 1524 Fragments arranged by size: 1524 837 787 597 436 393 336 268 226 EcoNI CCTnn'n_nnAGG Cuts at: 2423 2536 2423 Size: 113 5291 EcoO109I rG'GnC_Cy Cuts at: 2072 2073 2200 2392 2416 2072 Size: 1 127 192 24 5060 Fragments arranged by size: 5060 192 127 24 1 EcoRI G'AATT_C Cuts at: 2713 3091 2713 Size: 378 5026 FspI TGC'GCA Cuts at: 3910 3910 Size: 5404 HaeII r_GCGC'y Cuts at: 1678 2054 2136 2634 4551 1678 Size: 376 82 498 1917 2531 Fragments arranged by size: 2531 1917 498 376 82 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4045 4077 4119 4151 1373 Size: 32 135 32 2473 32 42 32 2626 Fragments arranged by size: 2626 2473 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2506 2748 2994 669 Size: 988 281 568 242 246 3079 Fragments arranged by size: 3079 988 568 281 246 242 HindIII A'AGCT_T Cuts at: 748 748 Size: 5404 HpaI GTT'AAC Cuts at: 2994 2994 Size: 5404 KasI G'GCGC_C Cuts at: 2132 2132 Size: 5404 KpnI G_GTAC'C Cuts at: 2729 2729 Size: 5404 MfeI C'AATT_G Cuts at: 3003 3003 Size: 5404 MluI A'CGCG_T Cuts at: 5280 5280 Size: 5404 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4518 4702 1305 Size: 3213 184 2007 Fragments arranged by size: 3213 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2345 10 Size: 54 1425 343 513 3069 Fragments arranged by size: 3069 1425 513 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2450 3362 3721 3880 518 Size: 648 1284 912 359 159 2042 Fragments arranged by size: 2042 1284 912 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2374 2608 2820 3497 4645 4890 1606 Size: 768 234 212 677 1148 245 2120 Fragments arranged by size: 2120 1148 768 677 245 234 212 NarI GG'CG_CC Cuts at: 2133 2133 Size: 5404 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4852 NdeI CA'TA_TG Cuts at: 387 387 Size: 5404 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5404 NotI GC'GGCC_GC Cuts at: 2851 2851 Size: 5404 NspI r_CATG'y Cuts at: 1132 1229 2762 4307 1132 Size: 97 1533 1545 2229 Fragments arranged by size: 2229 1545 1533 97 PasI CC'CwG_GG Cuts at: 1348 2077 2196 1348 Size: 729 119 4556 Fragments arranged by size: 4556 729 119 PciI A'CATG_T Cuts at: 4303 4303 Size: 5404 PflMI CCAn_nnn'nTGG Cuts at: 1108 2377 2571 1108 Size: 1269 194 3941 Fragments arranged by size: 3941 1269 194 PfoI T'CCnGG_A Cuts at: 2025 2025 Size: 5404 PmeI GTTT'AAAC Cuts at: 2708 2708 Size: 5404 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3473 3505 4986 5018 3473 Size: 32 1481 32 3859 Fragments arranged by size: 3859 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2200 2416 2200 Size: 216 5188 PshAI GACnn'nnGTC Cuts at: 1613 2501 1613 Size: 888 4516 PsiI TTA'TAA Cuts at: 2974 2974 Size: 5404 PspOMI G'GGCC_C Cuts at: 2072 2072 Size: 5404 PstI C_TGCA'G Cuts at: 830 2756 830 Size: 1926 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5404 PvuII CAG'CTG Cuts at: 2374 2608 2374 Size: 234 5170 SalI G'TCGA_C Cuts at: 1936 2746 1936 Size: 810 4594 SapI GCTCTTCn'nnn_ Cuts at: 2150 2150 Size: 5404 SbfI CC_TGCA'GG Cuts at: 2756 2756 Size: 5404 ScaI AGT'ACT Cuts at: 3652 5364 3652 Size: 1712 3692 SfcI C'TryA_G Cuts at: 826 1045 2268 2752 3887 4568 4759 826 Size: 219 1223 484 1135 681 191 1471 Fragments arranged by size: 1471 1223 1135 681 484 219 191 SfoI GGC'GCC Cuts at: 2134 2134 Size: 5404 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5404 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5404 SmaI CCC'GGG Cuts at: 1872 2731 1872 Size: 859 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2292 3508 4409 4671 820 Size: 197 926 349 1216 901 262 1553 Fragments arranged by size: 1553 1216 926 901 349 262 197 SnaBI TAC'GTA Cuts at: 493 3195 493 Size: 2702 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5404 SphI G_CATG'C Cuts at: 1132 2762 1132 Size: 1630 3774 SspI AAT'ATT Cuts at: 5 52 3328 5 Size: 47 3276 2081 Fragments arranged by size: 3276 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5404 StyI C'CwwG_G Cuts at: 513 1065 2431 2499 2843 513 Size: 552 1366 68 344 3074 Fragments arranged by size: 3074 1366 552 344 68 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2462 2477 2637 3431 3448 3601 3786 Size: 1255 882 15 160 794 17 153 185 Cuts at: 3786 4231 325 Size: 445 1498 Fragments arranged by size: 1498 1255 882 794 445 185 160 153 17 15 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3650 5362 96 Size: 274 80 33 51 1443 1673 1712 138 Fragments arranged by size: 1712 1673 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5364 5396 5364 Size: 32 5372 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5404 XbaI T'CTAG_A Cuts at: 2740 2740 Size: 5404 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2338 2338 Size: 5404 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5404 XmaI C'CCGG_G Cuts at: 1870 2729 1870 Size: 859 4545 XmnI GAAnn'nnTTC Cuts at: 3533 3533 Size: 5404 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3212 276 Size: 53 83 186 936 1678 2468 Fragments arranged by size: 2468 1678 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI