Restriction map for
pFN21AB7573
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# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII AccII AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsgI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstF5I BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI ClaI CpoI DraI DraIII
Eam1105I Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FokI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KspI MaeII MamI MfeI
MluI MluNI MroI MscI MseI
MspA1I MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tth111I XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 347 GGTACC G^GTACC
AccI 547 GTCTAC GT^[AC][GT]AC
AlwI 597 GATCC GGATC(4/5)
ApaI 394 GGGCCC GGGCC^C
Asp718I 347 GGTACC G^GTACC
AspHI 64 GAGCTC G[AT]GC[AT]^C
AvaII 570 GGTCC G^G[AT]CC
BpmI 459 CTGGAG CTGGAG(16/14)
BsaI 331 GAGACC GGTCTC(1/5)
BsiEI 143 CGGCCG CG[GA][CT]^CG
BsiHKAI 64 GAGCTC G[AT]GC[AT]^C
BsmFI 18 GTCCC GGGAC(10/14)
BspMI 612 GCAGGT ACCTGC(4/8)
BsrFI 618 GCCGGT [GA]^CCGG[CT]
BstAPI 609 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 596 AGATCC [GA]^GATC[CT]
Cfr10I 618 GCCGGT [GA]^CCGG[CT]
DpnII 597 GATC ^GATC
DraII 393 GGGGCCC [GA]G^G[ACGT]CC[CT]
DrdI 9 GACTTGTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 566 CCATGG C^C[GA][CT]GG
EagI 143 CGGCCG C^GGCCG
EarI 586 CTCTTC CTCTTC(1/4)
EclXI 143 CGGCCG C^GGCCG
Eco52I 143 CGGCCG C^GGCCG
EcoNI 366 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 393 GGGGCCC [GA]G^G[ACGT]CC[CT]
FauI 517 CCCGC CCCGC(4/6)
KpnI 347 GGTACC GGTAC^C
Ksp632I 586 CTCTTC CTCTTC(1/4)
MboI 597 GATC ^GATC
MboII 587 TCTTC GAAGA(8/7)
MflI 596 AGATCC [GA]^GATC[CT]
MslI 411 CATCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 609 GCAGCAGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 566 CCATGG C^CATGG
NdeII 597 GATC ^GATC
PaeR7I 472 CTCGAG C^TCGAG
PflMI 573 CCAGTACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 462 GAGTC GAGTC(4/5)
SacI 64 GAGCTC GAGCT^C
Sau3AI 597 GATC ^GATC
ScaI 353 AGTACT AGT^ACT
SexAI 343 ACCAGGT A^CC[AT]GGT
SfcI 481 CTACAG C^T[GA][CT]AG
TaqI 473 TCGA T^CGA
TfiI 630 GATTC G^A[AT]TC
Tsp509I 80 AATT ^AATT
TthHB8I 473 TCGA T^CGA
Van91I 573 CCAGTACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 472 CTCGAG C^TCGAG
XhoII 596 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
BfaI 449 CTAG C^TAG
493 CTAG
BsmAI 130 GAGAC GTCTC(1/5)
331 GAGAC
Cac8I 319 GCCAGC GC[ACGT]^[ACGT]GC
602 GCAAGC
DdeI 204 CTCAG C^T[ACGT]AG
314 CTAAG
EaeI 32 TGGCCG [CT]^GGCC[GA]
143 CGGCCG
HinfI 462 GAGTC G^A[ACGT]TC
630 GATTC
HphI 285 GGTGA GGTGA(8/7)
369 GGTGA
MaeI 449 CTAG C^TAG
493 CTAG
SmaI 335 CCCGGG CCC^GGG
404 CCCGGG
SmlI 292 CTCAAG C^T[CT][GA]AG
472 CTCGAG
TspRI 399 CAGTG CA[GC]TG[ACGT][ACGT]^
521 CAGTG
XmaCI 335 CCCGGG C^CCGGG
404 CCCGGG
XmaI 335 CCCGGG C^CCGGG
404 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AfaI AluI AvaI BanI
BanII BbvI BcnI BmyI BsaJI
BsiYI BslI BsoBI Bsp1286I BsrI
BssKI BstNI Cfr13I EcoRII EcoT14I
Fnu4HI HaeIII HapII HpaII ItaI
MaeIII MnlI MspI MvaI NciI
NlaIII NlaIV RsaI Sau96I ScrFI
SfaNI StyI TseI Tsp45I