Restriction map for
pFN21AB7573
(Linear) (Six-base) MAPSORT of: KIBB7573.seq Check: 640 from: 1 to: 636
KIBB7573.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 603 636
Size: 603 33
AccI GT'mk_AC
Cuts at: 0 548 636
Size: 548 88
Acc65I G'GTAC_C
Cuts at: 0 347 636
Size: 347 289
ApaI G_GGCC'C
Cuts at: 0 398 636
Size: 398 238
AvaI C'yCGr_G
Cuts at: 0 335 404 472 636
Size: 335 69 68 164
Fragments arranged by size:
335 164 69 68
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 554 587 636
Size: 554 33 49
Fragments arranged by size:
554 49 33
BanI G'GyrC_C
Cuts at: 0 340 347 615 636
Size: 340 7 268 21
Fragments arranged by size:
340 268 21 7
BanII G_rGCy'C
Cuts at: 0 68 398 516 636
Size: 68 330 118 120
Fragments arranged by size:
330 120 118 68
Bme1580I G_kGCm'C
Cuts at: 0 343 382 398 405 636
Size: 343 39 16 7 231
Fragments arranged by size:
343 231 39 16 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 392 636
Size: 392 244
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 480 636
Size: 480 156
Bpu10I CC'TnA_GC
Cuts at: 0 314 636
Size: 314 322
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 636
Size: 277 359
BsaI GGTCTCn'nnnn_
Cuts at: 0 325 636
Size: 325 311
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 412 442 636
Size: 412 30 194
Fragments arranged by size:
412 194 30
BseYI C'CCAG_C
Cuts at: 0 555 625 636
Size: 555 70 11
Fragments arranged by size:
555 70 11
BsiEI CG_ry'CG
Cuts at: 0 146 636
Size: 146 490
BsiHKAI G_wGCw'C
Cuts at: 0 68 636
Size: 68 568
Bsp1286I G_dGCh'C
Cuts at: 0 68 343 382 398 405 516 636
Size: 68 275 39 16 7 111 120
Fragments arranged by size:
275 120 111 68 39 16 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 603 636
Size: 603 33
BsrFI r'CCGG_y
Cuts at: 0 618 636
Size: 618 18
BstAPI GCAn_nnn'nTGC
Cuts at: 0 615 636
Size: 615 21
BstYI r'GATC_y
Cuts at: 0 596 636
Size: 596 40
BtgI C'CryG_G
Cuts at: 0 566 636
Size: 566 70
BtsI GCAGTG_nn'
Cuts at: 0 527 636
Size: 527 109
DrdI GACnn_nn'nnGTC
Cuts at: 0 15 636
Size: 15 621
EaeI y'GGCC_r
Cuts at: 0 32 143 636
Size: 32 111 493
Fragments arranged by size:
493 111 32
EagI C'GGCC_G
Cuts at: 0 143 636
Size: 143 493
EarI CTCTTCn'nnn_
Cuts at: 0 592 636
Size: 592 44
EcoICRI GAG'CTC
Cuts at: 0 66 636
Size: 66 570
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 480 636
Size: 480 156
EcoNI CCTnn'n_nnAGG
Cuts at: 0 370 636
Size: 370 266
EcoO109I rG'GnC_Cy
Cuts at: 0 394 636
Size: 394 242
KpnI G_GTAC'C
Cuts at: 0 351 636
Size: 351 285
MslI CAynn'nnrTG
Cuts at: 0 415 636
Size: 415 221
NcoI C'CATG_G
Cuts at: 0 566 636
Size: 566 70
PflMI CCAn_nnn'nTGG
Cuts at: 0 579 636
Size: 579 57
PspOMI G'GGCC_C
Cuts at: 0 394 636
Size: 394 242
PspXI vC'TCGA_Gb
Cuts at: 0 472 636
Size: 472 164
SacI G_AGCT'C
Cuts at: 0 68 636
Size: 68 568
ScaI AGT'ACT
Cuts at: 0 355 636
Size: 355 281
SexAI A'CCwGG_T
Cuts at: 0 343 636
Size: 343 293
SfcI C'TryA_G
Cuts at: 0 481 636
Size: 481 155
SmaI CCC'GGG
Cuts at: 0 337 406 636
Size: 337 69 230
Fragments arranged by size:
337 230 69
SmlI C'TyrA_G
Cuts at: 0 292 472 636
Size: 292 180 164
Fragments arranged by size:
292 180 164
StyI C'CwwG_G
Cuts at: 0 156 165 566 636
Size: 156 9 401 70
Fragments arranged by size:
401 156 70 9
TatI w'GTAC_w
Cuts at: 0 353 575 636
Size: 353 222 61
Fragments arranged by size:
353 222 61
XhoI C'TCGA_G
Cuts at: 0 472 636
Size: 472 164
XmaI C'CCGG_G
Cuts at: 0 335 404 636
Size: 335 69 232
Fragments arranged by size:
335 232 69
Enzymes that do cut:
AarI AccI Acc65I ApaI AvaI BaeI BanI BanII
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaXI BseYI
BsiEI BsiHKAI Bsp1286I BspMI BsrFI BstAPI BstYI BtgI
BtsI DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I KpnI MslI NcoI PflMI PspOMI PspXI SacI
ScaI SexAI SfcI SmaI SmlI StyI TatI XhoI
XmaI
Enzymes that do not cut:
AatII AclI AcuI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BamHI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI
BsaAI BsaBI BsaHI BsaWI BseRI BsgI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII EciI EcoRI EcoRV FalI FseI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI MfeI
MluI MmeI MscI MspA1I NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI PvuII RsrII SacII SalI SanDI SapI
SbfI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI
XcmI XmnI ZraI