Restriction map for
pFN21AB7563
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BbvI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiYI
BslI BsmBI BsmI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstF5I BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
Fnu4HI FokI FseI FspI HaeII
HaeIII HapII HincII HindII HpaI
HpaII HphI ItaI KasI KpnI
KspI MamI MflI MluI MluNI
MroI MscI MslI MspI MvnI
NaeI NarI NciI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI StuI SwaI TseI
Tsp45I Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AcsI 436 AAATTC [GA]^AATT[CT]
AcyI 91 GACGCC G[GA]^CG[CT]C
ApoI 436 AAATTC [GA]^AATT[CT]
AvaI 48 CTCGGG C^[CT]CG[GA]G
AvaII 559 GGACC G^G[AT]CC
BsaHI 91 GACGCC G[GA]^CG[CT]C
BseRI 236 GAGGAG GAGGAG(10/8)
BsiWI 11 CGTACG C^GTACG
BsmAI 445 GAGAC GTCTC(1/5)
BsmFI 185 GGGAC GGGAC(10/14)
BsoBI 48 CTCGGG C^[CT]CG[GA]G
BssKI 354 CCTGG ^CC[ACGT]GG
BssSI 497 CACGAG CACGAG(-5/-1)
BstAPI 59 GCAAGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 354 CCTGG CC^[AT]GG
CfoI 452 GCGC GCG^C
Cfr13I 559 GGACC G^G[ACGT]CC
DdeI 84 CTCAG C^T[ACGT]AG
DraI 133 TTTAAA TTT^AAA
DsaI 242 CCATGG C^C[GA][CT]GG
EcoRII 354 CCTGG ^CC[AT]GG
EcoT14I 242 CCATGG C^C[AT][AT]GG
FauI 193 GCGGG CCCGC(4/6)
HgaI 91 GACGC GACGC(5/10)
HhaI 452 GCGC GCG^C
Hin1I 91 GACGCC G[GA]^CG[CT]C
HinP1I 452 GCGC G^CGC
HindIII 172 AAGCTT A^AGCTT
MaeII 10 ACGT A^CGT
MaeIII 359 GTAAC ^GT[ACGT]AC
MfeI 419 CAATTG C^AATTG
MspA1I 191 CAGCGG C[AC]G^C[GT]G
MunI 419 CAATTG C^AATTG
MvaI 354 CCTGG CC^[AT]GG
MwoI 59 GCAAGACCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 242 CCATGG C^CATGG
NheI 414 GCTAGC G^CTAGC
NlaIV 238 GGAGCC GG[ACGT]^[ACGT]CC
NspI 324 GCATGC [GA]CATG^[CT]
PleI 46 GACTC GAGTC(4/5)
PshAI 386 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 319 GAAGAGC GCTCTTC(1/4)
Sau96I 559 GGACC G^G[ACGT]CC
ScrFI 354 CCTGG CC^[ACGT]GG
SfcI 231 CTACAG C^T[GA][CT]AG
SphI 324 GCATGC GCATG^C
SspI 292 AATATT AAT^ATT
StyI 242 CCATGG C^C[AT][AT]GG
TaqI 341 TCGA T^CGA
TfiI 206 GATTC G^A[AT]TC
TthHB8I 341 TCGA T^CGA
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# TWO CUT ENZYMES ( 13 enzymes) #
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AciI 78 CCGC CCGC(-3/-1)
193 GCGG
AfaI 12 GTAC GT^AC
228 GTAC
AlwI 351 GATCC GGATC(4/5)
305 GGATC
BbsI 6 GAAGAC GAAGAC(2/6)
591 GAAGAC
BfaI 154 CTAG C^TAG
415 CTAG
BpuAI 6 GAAGAC GAAGAC(2/6)
591 GAAGAC
BsaJI 242 CCATGG C^C[ACGT][ACGT]GG
354 CCTGGG
BspMI 64 ACCTGC ACCTGC(4/8)
553 GCAGGT
HinfI 46 GACTC G^A[ACGT]TC
206 GATTC
MaeI 154 CTAG C^TAG
415 CTAG
NlaIII 243 CATG CATG^
325 CATG
RsaI 12 GTAC GT^AC
228 GTAC
TspRI 462 CAGTG CA[GC]TG[ACGT][ACGT]^
563 CAGTG
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# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AluI BsrI Cac8I DpnII EarI
Ksp632I MboI MboII MnlI MseI
NdeII Sau3AI Tru9I Tsp509I