(Circular) (Six-base) MAPSORT of: pFN21AB7552.seq Check: 4301 from: 1 to: 5509 pFN21AB7552 5509 bp With 182 enzymes: SgfI * February 27, 2009 14:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3319 278 Size: 53 83 186 936 1783 2468 Fragments arranged by size: 2468 1783 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2852 1937 Size: 915 4594 Acc65I G'GTAC_C Cuts at: 2830 2830 Size: 5509 AclI AA'CG_TT Cuts at: 1563 3636 4009 1563 Size: 2073 373 3063 Fragments arranged by size: 3063 2073 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2546 3572 4956 5224 1632 Size: 914 1026 1384 268 1917 Fragments arranged by size: 1917 1384 1026 914 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5312 AflIII A'CryG_T Cuts at: 1203 2806 4408 5385 1203 Size: 1603 1602 977 1327 Fragments arranged by size: 1603 1602 1327 977 AgeI A'CCGG_T Cuts at: 4340 4340 Size: 5509 AhdI GACnn_n'nnGTC Cuts at: 4238 4238 Size: 5509 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5509 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2475 1184 Size: 1291 4218 AlwNI CAG_nnn'CTG Cuts at: 2595 3252 4824 2595 Size: 657 1572 3280 Fragments arranged by size: 3280 1572 657 ApaLI G'TGCA_C Cuts at: 3566 4722 3566 Size: 1156 4353 ApoI r'AATT_y Cuts at: 1493 2818 3044 3196 5305 1493 Size: 1325 226 152 2109 1697 Fragments arranged by size: 2109 1697 1325 226 152 AseI AT'TA_AT Cuts at: 160 1032 4063 160 Size: 872 3031 1606 Fragments arranged by size: 3031 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5509 AvaI C'yCGr_G Cuts at: 1870 1943 2834 1870 Size: 73 891 4545 Fragments arranged by size: 4545 891 73 BamHI G'GATC_C Cuts at: 2839 3206 2839 Size: 367 5142 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2519 2830 4285 618 Size: 325 217 627 732 311 1455 1842 Fragments arranged by size: 1842 1455 732 627 325 311 217 BanII G_rGCy'C Cuts at: 1391 2695 2828 1391 Size: 1304 133 4072 Fragments arranged by size: 4072 1304 133 BbeI G_GCGC'C Cuts at: 2523 2523 Size: 5509 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4538 BbvCI CC'TCA_GC Cuts at: 2147 2147 Size: 5509 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3700 3734 1903 Size: 34 50 34 1679 34 3678 Fragments arranged by size: 3678 1679 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2231 3401 4611 2231 Size: 1170 1210 3129 Fragments arranged by size: 3129 1210 1170 BclI T'GATC_A Cuts at: 1548 1548 Size: 5509 BfrBI ATG'CAT Cuts at: 2533 2533 Size: 5509 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4120 136 Size: 107 122 71 631 605 2448 1525 Fragments arranged by size: 2448 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2568 5504 2568 Size: 2936 2573 BlpI GC'TnA_GC Cuts at: 2926 2926 Size: 5509 Bme1580I G_kGCm'C Cuts at: 1790 3570 4726 1790 Size: 1780 1156 2573 Fragments arranged by size: 2573 1780 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5509 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2458 3240 4198 Size: 416 53 83 399 24 1041 782 958 Cuts at: 4198 442 Size: 1753 Fragments arranged by size: 1753 1041 958 782 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5509 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2131 2286 2131 Size: 155 5354 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2740 4169 1239 Size: 1501 1429 2579 Fragments arranged by size: 2579 1501 1429 Bpu10I CC'TnA_GC Cuts at: 2147 2152 2147 Size: 5 5504 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3634 4499 4797 3634 Size: 865 298 4346 Fragments arranged by size: 4346 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4172 882 Size: 617 2673 2219 Fragments arranged by size: 2673 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3300 493 Size: 711 2096 2702 Fragments arranged by size: 2702 2096 711 BsaBI GATnn'nnATC Cuts at: 5503 5503 Size: 5509 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2520 3316 3698 4333 Size: 53 83 186 936 987 796 382 635 Cuts at: 4333 275 Size: 1451 Fragments arranged by size: 1451 987 936 796 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2234 3942 4340 4614 4761 5172 2234 Size: 1708 398 274 147 411 2571 Fragments arranged by size: 2571 1708 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2176 2206 2273 2303 1178 Size: 30 165 30 773 30 67 30 4384 Fragments arranged by size: 4384 773 165 67 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2217 2233 2262 2278 1186 Size: 1031 16 29 16 4417 Fragments arranged by size: 4417 1031 29 16 16 BseYI C'CCAG_C Cuts at: 2205 4712 5208 2205 Size: 2507 496 2506 Fragments arranged by size: 2507 2506 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2435 2756 1163 Size: 573 699 321 3916 Fragments arranged by size: 3916 699 573 321 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2959 3720 4748 1540 Size: 275 186 958 761 1028 2301 Fragments arranged by size: 2301 1028 958 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3570 3655 4726 3570 Size: 85 1071 4353 Fragments arranged by size: 4353 1071 85 BsmI GAATG_Cn' Cuts at: 3020 3113 3020 Size: 93 5416 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2695 2828 3570 3655 4726 1391 Size: 399 905 133 742 85 1071 2174 Fragments arranged by size: 2174 1071 905 742 399 133 85 BspEI T'CCGG_A Cuts at: 2234 5172 2234 Size: 2938 2571 BspHI T'CATG_A Cuts at: 3398 5128 3398 Size: 1730 3779 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2864 844 Size: 2020 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3396 1394 Size: 429 1573 3507 Fragments arranged by size: 3507 1573 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4004 4178 65 Size: 1183 2756 174 1396 Fragments arranged by size: 2756 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4153 4340 1700 Size: 110 2343 187 2869 Fragments arranged by size: 2869 2343 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5509 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5509 BssSI C'ACGA_G Cuts at: 3569 4581 3569 Size: 1012 4497 BstAPI GCAn_nnn'nTGC Cuts at: 794 2400 2571 2739 794 Size: 1606 171 168 3564 Fragments arranged by size: 3564 1606 171 168 BstBI TT'CG_AA Cuts at: 4313 4313 Size: 5509 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5509 BstYI r'GATC_y Cuts at: 1972 2142 2568 2839 3206 3591 3608 5060 5146 Size: 170 426 271 367 385 17 1452 86 Cuts at: 5146 5158 5504 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 426 385 367 346 271 170 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4957 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2472 487 Size: 683 56 1246 3524 Fragments arranged by size: 3524 1246 683 56 BtsI GCAGTG_nn' Cuts at: 2452 2653 3025 3819 3839 2452 Size: 201 372 794 20 4122 Fragments arranged by size: 4122 794 372 201 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5509 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5474 DraI TTT'AAA Cuts at: 2813 2813 Size: 5509 DraIII CAC_nnn'GTG Cuts at: 2178 2178 Size: 5509 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4516 809 Size: 507 3200 1802 Fragments arranged by size: 3200 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2433 2956 3845 Size: 54 1425 50 275 18 603 523 889 Cuts at: 3845 5474 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 603 523 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2956 1537 Size: 275 1144 4090 Fragments arranged by size: 4090 1144 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2325 3439 1353 Size: 972 1114 3423 Fragments arranged by size: 3423 1114 972 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4096 4470 4616 387 Size: 1064 138 2507 374 146 1280 Fragments arranged by size: 2507 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2546 2740 3572 4169 4956 5224 1239 Size: 393 914 194 832 597 787 268 1524 Fragments arranged by size: 1524 914 832 787 597 393 268 194 EcoRI G'AATT_C Cuts at: 2818 3196 2818 Size: 378 5131 FspI TGC'GCA Cuts at: 4015 4015 Size: 5509 HaeII r_GCGC'y Cuts at: 1678 2523 4656 1678 Size: 845 2133 2531 Fragments arranged by size: 2531 2133 845 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2273 2305 2450 2482 2680 Size: 32 135 32 701 32 145 32 198 Cuts at: 2680 2712 4150 4182 4224 4256 1373 Size: 32 1438 32 42 32 2626 Fragments arranged by size: 2626 1438 701 198 145 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2853 3099 669 Size: 988 281 915 246 3079 Fragments arranged by size: 3079 988 915 281 246 HindIII A'AGCT_T Cuts at: 748 2351 748 Size: 1603 3906 HpaI GTT'AAC Cuts at: 3099 3099 Size: 5509 KasI G'GCGC_C Cuts at: 2519 2519 Size: 5509 KpnI G_GTAC'C Cuts at: 2834 2834 Size: 5509 MfeI C'AATT_G Cuts at: 3108 3108 Size: 5509 MluI A'CGCG_T Cuts at: 5385 5385 Size: 5509 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4623 4807 1305 Size: 3318 184 2007 Fragments arranged by size: 3318 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2435 10 Size: 54 1425 343 603 3084 Fragments arranged by size: 3084 1425 603 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2174 2457 2712 2778 3467 3826 3985 Size: 648 1008 283 255 66 689 359 159 Cuts at: 3985 518 Size: 2042 Fragments arranged by size: 2042 1008 689 648 359 283 255 159 66 MspA1I CmG'CkG Cuts at: 1606 2189 2449 2484 2925 3602 4750 4995 1606 Size: 583 260 35 441 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 583 441 260 245 35 NarI GG'CG_CC Cuts at: 2520 2520 Size: 5509 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4957 NdeI CA'TA_TG Cuts at: 387 387 Size: 5509 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5509 NotI GC'GGCC_GC Cuts at: 2956 2956 Size: 5509 NsiI A_TGCA'T Cuts at: 2535 2535 Size: 5509 NspI r_CATG'y Cuts at: 1132 1229 2867 4412 1132 Size: 97 1638 1545 2229 Fragments arranged by size: 2229 1638 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5509 PciI A'CATG_T Cuts at: 4408 4408 Size: 5509 PflMI CCAn_nnn'nTGG Cuts at: 1108 2595 1108 Size: 1487 4022 PmeI GTTT'AAAC Cuts at: 2813 2813 Size: 5509 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3578 3610 5091 5123 3578 Size: 32 1481 32 3964 Fragments arranged by size: 3964 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2344 1613 Size: 731 4778 PsiI TTA'TAA Cuts at: 3079 3079 Size: 5509 PstI C_TGCA'G Cuts at: 830 2703 2861 830 Size: 1873 158 3478 Fragments arranged by size: 3478 1873 158 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5509 PvuII CAG'CTG Cuts at: 2449 2484 2449 Size: 35 5474 SalI G'TCGA_C Cuts at: 1936 2851 1936 Size: 915 4594 SbfI CC_TGCA'GG Cuts at: 2861 2861 Size: 5509 ScaI AGT'ACT Cuts at: 3757 5469 3757 Size: 1712 3797 SfcI C'TryA_G Cuts at: 826 1045 2059 2342 2699 2857 3992 4673 4864 Size: 219 1014 283 357 158 1135 681 191 Cuts at: 4864 826 Size: 1471 Fragments arranged by size: 1471 1135 1014 681 357 283 219 191 158 SfoI GGC'GCC Cuts at: 2521 2521 Size: 5509 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5509 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5509 SmaI CCC'GGG Cuts at: 1872 2836 1872 Size: 964 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3613 4514 4776 820 Size: 197 926 1670 901 262 1553 Fragments arranged by size: 1670 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3300 493 Size: 2807 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5509 SphI G_CATG'C Cuts at: 1132 2867 1132 Size: 1735 3774 SspI AAT'ATT Cuts at: 5 52 3433 5 Size: 47 3381 2081 Fragments arranged by size: 3381 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5509 StyI C'CwwG_G Cuts at: 513 1065 2292 2948 513 Size: 552 1227 656 3074 Fragments arranged by size: 3074 1227 656 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3536 3553 3706 3891 4336 325 Size: 1255 1956 17 153 185 445 1498 Fragments arranged by size: 1956 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3755 5467 96 Size: 274 80 33 51 1443 1778 1712 138 Fragments arranged by size: 1778 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5469 5501 5469 Size: 32 5477 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5509 XbaI T'CTAG_A Cuts at: 2468 2845 2468 Size: 377 5132 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2213 2213 Size: 5509 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5509 XmaI C'CCGG_G Cuts at: 1870 2834 1870 Size: 964 4545 XmnI GAAnn'nnTTC Cuts at: 2033 2385 3638 2033 Size: 352 1253 3904 Fragments arranged by size: 3904 1253 352 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3317 276 Size: 53 83 186 936 1783 2468 Fragments arranged by size: 2468 1783 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI