Restriction map for
pFN21AB7541
##################################
# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaII AviII AvrII BalI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BlnI BpmI BpuAI
BsaAI BsaBI BsaI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HindIII HphI KpnI
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MslI MunI
NaeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccII 21 CGCG CG^CG
AfaI 135 GTAC GT^AC
AgeI 218 ACCGGT A^CCGGT
AlwNI 157 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 162 CCTGAGG CC^T[ACGT]AGG
BamHI 318 GGATCC G^GATCC
BanI 33 GGCGCC G^G[CT][GA]CC
BbeI 33 GGCGCC GGCGC^C
BfaI 404 CTAG C^TAG
BglI 514 GCCATGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 149 AGATCT A^GATCT
BlpI 61 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 61 GCTCAGC GC^T[ACGT]AGC
BsaWI 218 ACCGGT [AT]^CCGG[AT]
BsgI 121 GTGCAG GTGCAG(16/14)
BsmAI 115 GAGAC GTCTC(1/5)
BsrFI 218 ACCGGT [GA]^CCGG[CT]
BstNI 57 CCAGG CC^[AT]GG
BstUI 21 CGCG CG^CG
Bsu36I 162 CCTGAGG CC^T[ACGT]AGG
CelII 61 GCTCAGC GC^T[ACGT]AGC
CfoI 34 GCGC GCG^C
Cfr10I 218 ACCGGT [GA]^CCGG[CT]
DsaI 143 CCATGG C^C[GA][CT]GG
EaeI 17 TGGCCG [CT]^GGCC[GA]
Eco81I 162 CCTGAGG CC^T[ACGT]AGG
EcoRII 57 CCAGG ^CC[AT]GG
EcoT14I 143 CCATGG C^C[AT][AT]GG
HaeII 33 GGCGCC [GA]GCGC^[CT]
HhaI 34 GCGC GCG^C
HinP1I 34 GCGC G^CGC
HinfI 456 GAATC G^A[ACGT]TC
HpaI 222 GTTAAC GTT^AAC
KasI 33 GGCGCC G^GCGCC
MaeI 404 CTAG C^TAG
MaeIII 190 GTCAC ^GT[ACGT]AC
MseI 223 TTAA T^TAA
MvaI 57 CCAGG CC^[AT]GG
MvnI 21 CGCG CG^CG
NarI 33 GGCGCC GG^CGCC
NcoI 143 CCATGG C^CATGG
NdeI 347 CATATG CA^TATG
NspI 170 ACATGC [GA]CATG^[CT]
PinAI 218 ACCGGT A^CCGGT
RsaI 135 GTAC GT^AC
SfaNI 104 GCATC GCATC(5/9)
SfcI 232 CTACAG C^T[GA][CT]AG
SfoI 33 GGCGCC GGC^GCC
StyI 143 CCATGG C^C[AT][AT]GG
TaqI 332 TCGA T^CGA
TfiI 456 GAATC G^A[AT]TC
Tru9I 223 TTAA T^TAA
Tsp45I 190 GTCAC ^GT[GC]AC
TthHB8I 332 TCGA T^CGA
XcmI 5 CCACCCCTCCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 33 GGCGCC G[GA]^CG[CT]C
90 GGCGTC
ApaI 40 GGGCCC GGGCC^C
52 GGGCCC
AvaI 37 CCCGGG C^[CT]CG[GA]G
74 CCCGGG
BanII 40 GGGCCC G[GA]GC[CT]^C
52 GGGCCC
BbvI 174 GCAGC GCAGC(8/12)
177 GCTGC
BmyI 40 GGGCCC G[AGT]GC[ACT]^C
52 GGGCCC
BsaHI 33 GGCGCC G[GA]^CG[CT]C
90 GGCGTC
BsoBI 37 CCCGGG C^[CT]CG[GA]G
74 CCCGGG
Bsp1286I 40 GGGCCC G[AGT]GC[ACT]^C
52 GGGCCC
BsrDI 296 GCAATG GCAATG(2/0)
523 GCAATG
BsrI 202 ACTGG ACTGG(1/-1)
328 CCAGT
BstF5I 29 GGATG GGATG(2/0)
314 GGATG
BstYI 149 AGATCT [GA]^GATC[CT]
318 GGATCC
Cac8I 66 GCAGGC GC[ACGT]^[ACGT]GC
385 GCTGGC
DraII 40 GGGCCCT [GA]G^G[ACGT]CC[CT]
52 GGGCCCC
EarI 262 CTCTTC CTCTTC(1/4)
409 GAAGAG
EcoO109I 40 GGGCCCT [GA]G^G[ACGT]CC[CT]
52 GGGCCCC
FokI 29 GGATG GGATG(9/13)
314 GGATG
HgaI 22 GCGTC GACGC(5/10)
91 GCGTC
Hin1I 33 GGCGCC G[GA]^CG[CT]C
90 GGCGTC
HincII 82 GTCAAC GT[CT]^[GA]AC
222 GTTAAC
HindII 82 GTCAAC GT[CT]^[GA]AC
222 GTTAAC
Ksp632I 262 CTCTTC CTCTTC(1/4)
409 GAAGAG
MflI 149 AGATCT [GA]^GATC[CT]
318 GGATCC
MspA1I 175 CAGCTG C[AC]G^C[GT]G
463 CAGCTG
MwoI 61 GCTCAGCAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
514 GCCATGTTGGC
PvuII 175 CAGCTG CAG^CTG
463 CAGCTG
SmaI 37 CCCGGG CCC^GGG
74 CCCGGG
TseI 174 GCAGC G^C[AT]GC
177 GCTGC
TspRI 119 CAGTG CA[GC]TG[ACGT][ACGT]^
201 CACTG
XhoII 149 AGATCT [GA]^GATC[CT]
318 GGATCC
XmaCI 37 CCCGGG C^CCGGG
74 CCCGGG
XmaI 37 CCCGGG C^CCGGG
74 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AluI AlwI BcnI BsaJI
BsiYI BslI BssKI Cfr13I DdeI
DpnII Fnu4HI HaeIII HapII HpaII
ItaI MboI MboII MnlI MspI
NciI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI Tsp509I