(Circular) (Six-base) MAPSORT of: pFN21AB7541.seq Check: 9360 from: 1 to: 5239 pFN21AB7541 5239 bp With 182 enzymes: SgfI * February 27, 2009 14:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3049 278 Size: 53 83 186 936 1513 2468 Fragments arranged by size: 2468 1513 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2582 1937 Size: 645 4594 Acc65I G'GTAC_C Cuts at: 2560 2560 Size: 5239 AclI AA'CG_TT Cuts at: 1563 2538 3366 3739 1563 Size: 975 828 373 3063 Fragments arranged by size: 3063 975 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2254 2454 3302 4686 4954 1632 Size: 622 200 848 1384 268 1917 Fragments arranged by size: 1917 1384 848 622 268 200 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5042 AflIII A'CryG_T Cuts at: 1203 4138 5115 1203 Size: 2935 977 1327 Fragments arranged by size: 2935 1327 977 AgeI A'CCGG_T Cuts at: 2223 4070 2223 Size: 1847 3392 AhdI GACnn_n'nnGTC Cuts at: 3968 3968 Size: 5239 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5239 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5239 AlwNI CAG_nnn'CTG Cuts at: 2167 2982 4554 2167 Size: 815 1572 2852 Fragments arranged by size: 2852 1572 815 ApaI G_GGCC'C Cuts at: 2049 2061 2049 Size: 12 5227 ApaLI G'TGCA_C Cuts at: 3296 4452 3296 Size: 1156 4083 ApoI r'AATT_y Cuts at: 1493 2548 2774 2926 5035 1493 Size: 1055 226 152 2109 1697 Fragments arranged by size: 2109 1697 1055 226 152 AseI AT'TA_AT Cuts at: 160 1032 3793 160 Size: 872 2761 1606 Fragments arranged by size: 2761 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5239 AvaI C'yCGr_G Cuts at: 1870 1943 2042 2079 2564 1870 Size: 73 99 37 485 4545 Fragments arranged by size: 4545 485 99 73 37 BamHI G'GATC_C Cuts at: 2323 2569 2936 2323 Size: 246 367 4626 Fragments arranged by size: 4626 367 246 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2038 2560 4015 618 Size: 325 217 627 251 522 1455 1842 Fragments arranged by size: 1842 1455 627 522 325 251 217 BanII G_rGCy'C Cuts at: 1391 2049 2061 2558 1391 Size: 658 12 497 4072 Fragments arranged by size: 4072 658 497 12 BbeI G_GCGC'C Cuts at: 2042 2042 Size: 5239 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4268 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3430 3464 1903 Size: 34 1493 34 3678 Fragments arranged by size: 3678 1493 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3131 4341 3131 Size: 1210 4029 BclI T'GATC_A Cuts at: 1548 1548 Size: 5239 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2525 3850 136 Size: 107 122 71 631 605 853 1325 1525 Fragments arranged by size: 1525 1325 853 631 605 122 107 71 BglII A'GATC_T Cuts at: 2154 5234 2154 Size: 3080 2159 BlpI GC'TnA_GC Cuts at: 2067 2656 2067 Size: 589 4650 Bme1580I G_kGCm'C Cuts at: 1790 2049 2061 3300 4456 1790 Size: 259 12 1239 1156 2573 Fragments arranged by size: 2573 1239 1156 259 12 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5239 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2970 3928 442 Size: 416 53 83 399 24 1553 958 1753 Fragments arranged by size: 1753 1553 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5239 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3899 1239 Size: 2660 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3364 4229 4527 3364 Size: 865 298 4076 Fragments arranged by size: 4076 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3902 882 Size: 617 2403 2219 Fragments arranged by size: 2403 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3030 493 Size: 711 1826 2702 Fragments arranged by size: 2702 1826 711 BsaBI GATnn'nnATC Cuts at: 5233 5233 Size: 5239 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2039 2096 3046 3428 Size: 53 83 186 936 506 57 950 382 Cuts at: 3428 4063 275 Size: 635 1451 Fragments arranged by size: 1451 950 936 635 506 382 186 83 57 53 BsaWI w'CCGG_w Cuts at: 2223 3672 4070 4344 4491 4902 2223 Size: 1449 398 274 147 411 2560 Fragments arranged by size: 2560 1449 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5014 Fragments arranged by size: 5014 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5239 BseYI C'CCAG_C Cuts at: 4442 4938 4442 Size: 496 4743 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2147 1163 Size: 573 411 4255 Fragments arranged by size: 4255 573 411 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2689 3450 4478 1540 Size: 275 186 688 761 1028 2301 Fragments arranged by size: 2301 1028 761 688 275 186 BsiHKAI G_wGCw'C Cuts at: 3300 3385 4456 3300 Size: 85 1071 4083 Fragments arranged by size: 4083 1071 85 BsmI GAATG_Cn' Cuts at: 2750 2843 2750 Size: 93 5146 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2061 2558 3300 3385 4456 1391 Size: 399 259 12 497 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 497 399 259 85 12 BspEI T'CCGG_A Cuts at: 4902 4902 Size: 5239 BspHI T'CATG_A Cuts at: 3128 4858 3128 Size: 1730 3509 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2594 844 Size: 1750 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3126 1394 Size: 429 1303 3507 Fragments arranged by size: 3507 1303 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2308 2535 3734 3908 65 Size: 1183 1060 227 1199 174 1396 Fragments arranged by size: 1396 1199 1183 1060 227 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2223 3883 4070 1700 Size: 110 413 1660 187 2869 Fragments arranged by size: 2869 1660 413 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5239 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5239 BssSI C'ACGA_G Cuts at: 3299 4311 3299 Size: 1012 4227 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5239 BstBI TT'CG_AA Cuts at: 4043 4043 Size: 5239 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5239 BstYI r'GATC_y Cuts at: 1972 2154 2323 2569 2936 3321 3338 4790 4876 Size: 182 169 246 367 385 17 1452 86 Cuts at: 4876 4888 5234 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 246 182 169 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2168 2168 Size: 5239 BtgI C'CryG_G Cuts at: 513 1065 2148 513 Size: 552 1083 3604 Fragments arranged by size: 3604 1083 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4500 Fragments arranged by size: 4500 683 56 BtsI GCAGTG_nn' Cuts at: 2755 3549 3569 2755 Size: 794 20 4425 Fragments arranged by size: 4425 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5239 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5204 DraI TTT'AAA Cuts at: 2543 2543 Size: 5239 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4246 809 Size: 507 2930 1802 Fragments arranged by size: 2930 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2022 2686 3575 Size: 54 1425 50 275 18 192 664 889 Cuts at: 3575 5204 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 664 275 192 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2686 1537 Size: 275 874 4090 Fragments arranged by size: 4090 874 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2273 2409 3169 1353 Size: 920 136 760 3423 Fragments arranged by size: 3423 920 760 136 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3826 4200 4346 387 Size: 1064 138 2237 374 146 1280 Fragments arranged by size: 2237 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2254 2454 3302 3899 4686 4954 1239 Size: 393 622 200 848 597 787 268 1524 Fragments arranged by size: 1524 848 787 622 597 393 268 200 EcoO109I rG'GnC_Cy Cuts at: 2046 2058 2046 Size: 12 5227 EcoRI G'AATT_C Cuts at: 2548 2926 2548 Size: 378 4861 FspI TGC'GCA Cuts at: 3745 3745 Size: 5239 HaeII r_GCGC'y Cuts at: 1678 2042 4386 1678 Size: 364 2344 2531 Fragments arranged by size: 2531 2344 364 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3880 3912 3954 3986 1373 Size: 32 135 32 2308 32 42 32 2626 Fragments arranged by size: 2626 2308 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2089 2229 2583 2829 669 Size: 988 281 151 140 354 246 3079 Fragments arranged by size: 3079 988 354 281 246 151 140 HindIII A'AGCT_T Cuts at: 748 748 Size: 5239 HpaI GTT'AAC Cuts at: 2229 2829 2229 Size: 600 4639 KasI G'GCGC_C Cuts at: 2038 2038 Size: 5239 KpnI G_GTAC'C Cuts at: 2564 2564 Size: 5239 MfeI C'AATT_G Cuts at: 2838 2838 Size: 5239 MluI A'CGCG_T Cuts at: 5115 5115 Size: 5239 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4353 4537 1305 Size: 3048 184 2007 Fragments arranged by size: 3048 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3417 Fragments arranged by size: 3417 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3197 3556 3715 518 Size: 648 2031 359 159 2042 Fragments arranged by size: 2042 2031 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2182 2470 2655 3332 4480 4725 1606 Size: 576 288 185 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 576 288 245 185 NarI GG'CG_CC Cuts at: 2039 2039 Size: 5239 NcoI C'CATG_G Cuts at: 513 1065 2148 513 Size: 552 1083 3604 Fragments arranged by size: 3604 1083 552 NdeI CA'TA_TG Cuts at: 387 2353 387 Size: 1966 3273 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5239 NotI GC'GGCC_GC Cuts at: 2686 2686 Size: 5239 NspI r_CATG'y Cuts at: 1132 1229 2179 2597 4142 1132 Size: 97 950 418 1545 2229 Fragments arranged by size: 2229 1545 950 418 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5239 PciI A'CATG_T Cuts at: 4138 4138 Size: 5239 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5239 PmeI GTTT'AAAC Cuts at: 2543 2543 Size: 5239 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3308 3340 4821 4853 3308 Size: 32 1481 32 3694 Fragments arranged by size: 3694 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5239 PsiI TTA'TAA Cuts at: 2809 2809 Size: 5239 PspOMI G'GGCC_C Cuts at: 2045 2057 2045 Size: 12 5227 PstI C_TGCA'G Cuts at: 830 2591 830 Size: 1761 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5239 PvuII CAG'CTG Cuts at: 2182 2470 2182 Size: 288 4951 SalI G'TCGA_C Cuts at: 1936 2581 1936 Size: 645 4594 SbfI CC_TGCA'GG Cuts at: 2591 2591 Size: 5239 ScaI AGT'ACT Cuts at: 3487 5199 3487 Size: 1712 3527 SfcI C'TryA_G Cuts at: 826 1045 2237 2587 3722 4403 4594 826 Size: 219 1192 350 1135 681 191 1471 Fragments arranged by size: 1471 1192 1135 681 350 219 191 SfoI GGC'GCC Cuts at: 2040 2040 Size: 5239 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5239 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5239 SmaI CCC'GGG Cuts at: 1872 2044 2081 2566 1872 Size: 172 37 485 4545 Fragments arranged by size: 4545 485 172 37 SmlI C'TyrA_G Cuts at: 820 1017 1943 3343 4244 4506 820 Size: 197 926 1400 901 262 1553 Fragments arranged by size: 1553 1400 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3030 493 Size: 2537 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5239 SphI G_CATG'C Cuts at: 1132 2597 1132 Size: 1465 3774 SrfI GCCC'GGGC Cuts at: 2044 2044 Size: 5239 SspI AAT'ATT Cuts at: 5 52 3163 5 Size: 47 3111 2081 Fragments arranged by size: 3111 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5239 StyI C'CwwG_G Cuts at: 513 1065 2148 2678 513 Size: 552 1083 530 3074 Fragments arranged by size: 3074 1083 552 530 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3266 3283 3436 3621 4066 325 Size: 1255 1686 17 153 185 445 1498 Fragments arranged by size: 1686 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3485 5197 96 Size: 274 80 33 51 1443 1508 1712 138 Fragments arranged by size: 1712 1508 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5199 5231 5199 Size: 32 5207 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5239 XbaI T'CTAG_A Cuts at: 2575 2575 Size: 5239 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2017 2017 Size: 5239 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5239 XmaI C'CCGG_G Cuts at: 1870 2042 2079 2564 1870 Size: 172 37 485 4545 Fragments arranged by size: 4545 485 172 37 XmnI GAAnn'nnTTC Cuts at: 3368 3368 Size: 5239 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3047 276 Size: 53 83 186 936 1513 2468 Fragments arranged by size: 2468 1513 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SwaI