Restriction map for
pFN21AB7515
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# NO CUT ENZYMES ( 172 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiWI BsmAI
BsmBI BsmFI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I CelII ClaI CpoI DraI
DraII DraIII DsaI Eam1105I EarI
Eco47III EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HincII HindII HindIII HinfI HpaI
HphI KpnI Ksp632I KspI MaeI
MaeII MaeIII MamI MfeI MflI
MluI MluNI MroI MscI MseI
MslI MunI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TfiI Tru9I Tsp45I Tsp509I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccII 48 CGCG CG^CG
AcyI 51 GGCGCC G[GA]^CG[CT]C
AocI 94 CCTGAGG CC^T[ACGT]AGG
AspHI 289 GAGCAC G[AT]GC[AT]^C
AvaI 55 CCCGGG C^[CT]CG[GA]G
BanI 51 GGCGCC G^G[CT][GA]CC
BbeI 51 GGCGCC GGCGC^C
BglI 342 GCCTCCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 208 CTGGAG CTGGAG(16/14)
BsaHI 51 GGCGCC G[GA]^CG[CT]C
BsiHKAI 289 GAGCAC G[AT]GC[AT]^C
BsoBI 55 CCCGGG C^[CT]CG[GA]G
BsrI 337 CCAGT ACTGG(1/-1)
BssSI 292 CACGAG CACGAG(-5/-1)
BstF5I 99 GGATG GGATG(2/0)
BstNI 207 CCTGG CC^[AT]GG
BstUI 48 CGCG CG^CG
Bsu36I 94 CCTGAGG CC^T[ACGT]AGG
CfoI 52 GCGC GCG^C
DpnII 240 GATC ^GATC
DrdI 106 GACAGGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 94 CCTGAGG CC^T[ACGT]AGG
EcoNI 270 CCTGACAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 207 CCTGG ^CC[AT]GG
FauI 306 CCCGC CCCGC(4/6)
FokI 99 GGATG GGATG(9/13)
HaeII 51 GGCGCC [GA]GCGC^[CT]
HhaI 52 GCGC GCG^C
Hin1I 51 GGCGCC G[GA]^CG[CT]C
HinP1I 52 GCGC G^CGC
KasI 51 GGCGCC G^GCGCC
MboI 240 GATC ^GATC
MspA1I 307 CCGCTG C[AC]G^C[GT]G
MvaI 207 CCTGG CC^[AT]GG
MvnI 48 CGCG CG^CG
NarI 51 GGCGCC GG^CGCC
NdeII 240 GATC ^GATC
PstI 34 CTGCAG CTGCA^G
Sau3AI 240 GATC ^GATC
SfcI 34 CTGCAG C^T[GA][CT]AG
SfoI 51 GGCGCC GGC^GCC
SmaI 55 CCCGGG CCC^GGG
XmaCI 55 CCCGGG C^CCGGG
XmaI 55 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AvaII 114 GGTCC G^G[AT]CC
354 GGACC
BanII 10 GGGCTC G[GA]GC[CT]^C
90 GAGCCC
BsaJI 55 CCCGGG C^C[ACGT][ACGT]GG
346 CCCCGG
BseRI 39 GAGGAG GAGGAG(10/8)
256 GAGGAG
BsiEI 17 CGGCCG CG[GA][CT]^CG
349 CGGCCG
BsrFI 22 GCCGGC [GA]^CCGG[CT]
364 GCCGGC
Cfr10I 22 GCCGGC [GA]^CCGG[CT]
364 GCCGGC
Cfr13I 114 GGTCC G^G[ACGT]CC
354 GGACC
DdeI 95 CTGAG C^T[ACGT]AG
146 CTCAG
EagI 17 CGGCCG C^GGCCG
349 CGGCCG
EclXI 17 CGGCCG C^GGCCG
349 CGGCCG
Eco52I 17 CGGCCG C^GGCCG
349 CGGCCG
HgaI 29 GCGTC GACGC(5/10)
44 GCGTC
MboII 159 GAAGA GAAGA(8/7)
297 GAAGA
NaeI 22 GCCGGC GCC^GGC
364 GCCGGC
NgoMIV 22 GCCGGC G^CCGGC
364 GCCGGC
NlaIII 190 CATG CATG^
225 CATG
Sau96I 114 GGTCC G^G[ACGT]CC
354 GGACC
TspRI 281 CACTG CA[GC]TG[ACGT][ACGT]^
338 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AciI AluI BbvI BcnI BmyI
BsiYI BslI Bsp1286I BssKI Cac8I
EaeI Fnu4HI HaeIII HapII HpaII
ItaI MnlI MspI MwoI NciI
NlaIV ScrFI TseI