(Circular) (Six-base) MAPSORT of: pFN21AB7510.seq Check: 5968 from: 1 to: 5431 pFN21AB7510 5431 bp With 182 enzymes: SgfI * February 27, 2009 14:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3241 278 Size: 53 83 186 936 1705 2468 Fragments arranged by size: 2468 1705 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2774 1937 Size: 837 4594 Acc65I G'GTAC_C Cuts at: 2282 2752 2282 Size: 470 4961 AclI AA'CG_TT Cuts at: 1563 3558 3931 1563 Size: 1995 373 3063 Fragments arranged by size: 3063 1995 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2029 2363 3494 4878 5146 1632 Size: 397 334 1131 1384 268 1917 Fragments arranged by size: 1917 1384 1131 397 334 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5234 AflIII A'CryG_T Cuts at: 1203 4330 5307 1203 Size: 3127 977 1327 Fragments arranged by size: 3127 1327 977 AgeI A'CCGG_T Cuts at: 4262 4262 Size: 5431 AhdI GACnn_n'nnGTC Cuts at: 4160 4160 Size: 5431 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5431 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5431 AlwNI CAG_nnn'CTG Cuts at: 2131 3174 4746 2131 Size: 1043 1572 2816 Fragments arranged by size: 2816 1572 1043 ApaLI G'TGCA_C Cuts at: 3488 4644 3488 Size: 1156 4275 ApoI r'AATT_y Cuts at: 1493 2477 2740 2966 3118 5227 1493 Size: 984 263 226 152 2109 1697 Fragments arranged by size: 2109 1697 984 263 226 152 AseI AT'TA_AT Cuts at: 160 1032 3985 160 Size: 872 2953 1606 Fragments arranged by size: 2953 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5431 AvaI C'yCGr_G Cuts at: 1870 1943 2756 1870 Size: 73 813 4545 Fragments arranged by size: 4545 813 73 BamHI G'GATC_C Cuts at: 2761 3128 2761 Size: 367 5064 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2078 2282 2752 4207 618 Size: 325 217 627 291 204 470 1455 1842 Fragments arranged by size: 1842 1455 627 470 325 291 217 204 BanII G_rGCy'C Cuts at: 1391 2750 1391 Size: 1359 4072 BbeI G_GCGC'C Cuts at: 2082 2082 Size: 5431 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2046 928 Size: 971 147 4313 Fragments arranged by size: 4313 971 147 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2516 2550 3622 3656 1903 Size: 34 579 34 1072 34 3678 Fragments arranged by size: 3678 1072 579 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3323 4533 3323 Size: 1210 4221 BclI T'GATC_A Cuts at: 1548 1548 Size: 5431 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4042 136 Size: 107 122 71 631 605 2370 1525 Fragments arranged by size: 2370 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5426 5426 Size: 5431 BlpI GC'TnA_GC Cuts at: 2848 2848 Size: 5431 Bme1580I G_kGCm'C Cuts at: 1790 3492 4648 1790 Size: 1702 1156 2573 Fragments arranged by size: 2573 1702 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5431 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3162 4120 442 Size: 416 53 83 399 24 1745 958 1753 Fragments arranged by size: 1753 1745 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5431 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2141 2388 2526 2699 4091 1239 Size: 902 247 138 173 1392 2579 Fragments arranged by size: 2579 1392 902 247 173 138 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2708 3556 4421 4719 2708 Size: 848 865 298 3420 Fragments arranged by size: 3420 865 848 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2644 4094 882 Size: 617 1145 1450 2219 Fragments arranged by size: 2219 1450 1145 617 BsaAI yAC'GTr Cuts at: 493 1204 3222 493 Size: 711 2018 2702 Fragments arranged by size: 2702 2018 711 BsaBI GATnn'nnATC Cuts at: 5425 5425 Size: 5431 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2079 3238 3620 4255 Size: 53 83 186 936 546 1159 382 635 Cuts at: 4255 275 Size: 1451 Fragments arranged by size: 1451 1159 936 635 546 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3864 4262 4536 4683 5094 3864 Size: 398 274 147 411 4201 Fragments arranged by size: 4201 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5206 Fragments arranged by size: 5206 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5431 BseYI C'CCAG_C Cuts at: 2300 4634 5130 2300 Size: 2334 496 2601 Fragments arranged by size: 2601 2334 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4858 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2881 3642 4670 1540 Size: 275 186 880 761 1028 2301 Fragments arranged by size: 2301 1028 880 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2106 3492 3577 4648 2106 Size: 1386 85 1071 2889 Fragments arranged by size: 2889 1386 1071 85 BsmI GAATG_Cn' Cuts at: 2599 2942 3035 2599 Size: 343 93 4995 Fragments arranged by size: 4995 343 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2106 2750 3492 3577 4648 1391 Size: 399 316 644 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 644 399 316 85 BspEI T'CCGG_A Cuts at: 5094 5094 Size: 5431 BspHI T'CATG_A Cuts at: 2257 3320 5050 2257 Size: 1063 1730 2638 Fragments arranged by size: 2638 1730 1063 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2786 844 Size: 1942 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3318 1394 Size: 429 1495 3507 Fragments arranged by size: 3507 1495 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2637 3926 4100 65 Size: 1183 1389 1289 174 1396 Fragments arranged by size: 1396 1389 1289 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2093 4075 4262 1700 Size: 110 283 1982 187 2869 Fragments arranged by size: 2869 1982 283 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5431 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5431 BssSI C'ACGA_G Cuts at: 3491 4503 3491 Size: 1012 4419 BstAPI GCAn_nnn'nTGC Cuts at: 794 2605 794 Size: 1811 3620 BstBI TT'CG_AA Cuts at: 4235 4235 Size: 5431 BstEII G'GTnAC_C Cuts at: 1184 2614 1184 Size: 1430 4001 BstXI CCAn_nnnn'nTGG Cuts at: 2547 2586 2547 Size: 39 5392 BstYI r'GATC_y Cuts at: 1972 2761 3128 3513 3530 4982 5068 5080 5426 Size: 789 367 385 17 1452 86 12 346 Cuts at: 5426 1972 Size: 1977 Fragments arranged by size: 1977 1452 789 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2619 513 Size: 552 939 615 3325 Fragments arranged by size: 3325 939 615 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4692 Fragments arranged by size: 4692 683 56 BtsI GCAGTG_nn' Cuts at: 2947 3741 3761 2947 Size: 794 20 4617 Fragments arranged by size: 4617 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5431 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5396 DraI TTT'AAA Cuts at: 2735 2735 Size: 5431 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4438 809 Size: 507 3122 1802 Fragments arranged by size: 3122 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2155 2878 Size: 54 1425 50 275 18 177 148 723 Cuts at: 2878 3767 5396 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 723 275 177 148 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2878 1537 Size: 275 1066 4090 Fragments arranged by size: 4090 1066 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3361 1353 Size: 2008 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2321 4018 4392 4538 387 Size: 1064 138 732 1697 374 146 1280 Fragments arranged by size: 1697 1280 1064 732 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2029 2141 2363 2388 2526 2699 3494 Size: 393 397 112 222 25 138 173 795 Cuts at: 3494 4091 4878 5146 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 795 787 597 397 393 268 222 173 138 112 25 EcoNI CCTnn'n_nnAGG Cuts at: 2123 2123 Size: 5431 EcoO109I rG'GnC_Cy Cuts at: 2128 2128 Size: 5431 EcoRI G'AATT_C Cuts at: 2740 3118 2740 Size: 378 5053 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2188 2188 Size: 5431 FspI TGC'GCA Cuts at: 3937 3937 Size: 5431 HaeII r_GCGC'y Cuts at: 1678 2082 4578 1678 Size: 404 2496 2531 Fragments arranged by size: 2531 2496 404 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2225 2257 2474 2506 4072 Size: 32 135 32 653 32 217 32 1566 Cuts at: 4072 4104 4146 4178 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1566 653 217 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2326 2775 3021 669 Size: 988 281 388 449 246 3079 Fragments arranged by size: 3079 988 449 388 281 246 HindIII A'AGCT_T Cuts at: 748 2030 2184 748 Size: 1282 154 3995 Fragments arranged by size: 3995 1282 154 HpaI GTT'AAC Cuts at: 3021 3021 Size: 5431 KasI G'GCGC_C Cuts at: 2078 2078 Size: 5431 KpnI G_GTAC'C Cuts at: 2286 2756 2286 Size: 470 4961 MfeI C'AATT_G Cuts at: 3030 3030 Size: 5431 MluI A'CGCG_T Cuts at: 5307 5307 Size: 5431 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2208 2245 4545 4729 1305 Size: 903 37 2300 184 2007 Fragments arranged by size: 2300 2007 903 184 37 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3609 Fragments arranged by size: 3609 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3389 3748 3907 518 Size: 648 2223 359 159 2042 Fragments arranged by size: 2223 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2085 2847 3524 4672 4917 1606 Size: 479 762 677 1148 245 2120 Fragments arranged by size: 2120 1148 762 677 479 245 NarI GG'CG_CC Cuts at: 2079 2079 Size: 5431 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3940 Fragments arranged by size: 3940 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5431 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5431 NotI GC'GGCC_GC Cuts at: 2878 2878 Size: 5431 NspI r_CATG'y Cuts at: 1132 1229 2789 4334 1132 Size: 97 1560 1545 2229 Fragments arranged by size: 2229 1560 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5431 PciI A'CATG_T Cuts at: 4330 4330 Size: 5431 PflMI CCAn_nnn'nTGG Cuts at: 1108 2701 1108 Size: 1593 3838 PfoI T'CCnGG_A Cuts at: 2118 2118 Size: 5431 PmeI GTTT'AAAC Cuts at: 2735 2735 Size: 5431 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3500 3532 5013 5045 3500 Size: 32 1481 32 3886 Fragments arranged by size: 3886 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5431 PsiI TTA'TAA Cuts at: 2190 3001 2190 Size: 811 4620 PstI C_TGCA'G Cuts at: 830 2353 2783 830 Size: 1523 430 3478 Fragments arranged by size: 3478 1523 430 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5431 SalI G'TCGA_C Cuts at: 1936 2773 1936 Size: 837 4594 SbfI CC_TGCA'GG Cuts at: 2783 2783 Size: 5431 ScaI AGT'ACT Cuts at: 3679 5391 3679 Size: 1712 3719 SfcI C'TryA_G Cuts at: 826 1045 2349 2779 3914 4595 4786 826 Size: 219 1304 430 1135 681 191 1471 Fragments arranged by size: 1471 1304 1135 681 430 219 191 SfoI GGC'GCC Cuts at: 2080 2080 Size: 5431 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5431 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5431 SmaI CCC'GGG Cuts at: 1872 2758 1872 Size: 886 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2723 3535 4436 4698 820 Size: 197 926 780 812 901 262 1553 Fragments arranged by size: 1553 926 901 812 780 262 197 SnaBI TAC'GTA Cuts at: 493 3222 493 Size: 2729 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5431 SphI G_CATG'C Cuts at: 1132 2789 1132 Size: 1657 3774 SspI AAT'ATT Cuts at: 5 52 3355 5 Size: 47 3303 2081 Fragments arranged by size: 3303 2081 47 StuI AGG'CCT Cuts at: 1517 2503 1517 Size: 986 4445 StyI C'CwwG_G Cuts at: 513 1065 2004 2460 2870 513 Size: 552 939 456 410 3074 Fragments arranged by size: 3074 939 552 456 410 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2343 3458 3475 3628 3813 4258 325 Size: 1255 763 1115 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1115 763 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3677 5389 96 Size: 274 80 33 51 1443 1700 1712 138 Fragments arranged by size: 1712 1700 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5391 5423 5391 Size: 32 5399 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5431 XbaI T'CTAG_A Cuts at: 2767 2767 Size: 5431 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2698 2698 Size: 5431 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5431 XmaI C'CCGG_G Cuts at: 1870 2756 1870 Size: 886 4545 XmnI GAAnn'nnTTC Cuts at: 3560 3560 Size: 5431 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3239 276 Size: 53 83 186 936 1705 2468 Fragments arranged by size: 2468 1705 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI