(Circular) (Six-base) MAPSORT of: pFN21AB7501.seq Check: 1094 from: 1 to: 4879 pFN21AB7501 4879 bp With 182 enzymes: SgfI * February 27, 2009 14:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2689 278 Size: 53 83 186 936 1153 2468 Fragments arranged by size: 2468 1153 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2222 1937 Size: 285 4594 Acc65I G'GTAC_C Cuts at: 2200 2200 Size: 4879 AclI AA'CG_TT Cuts at: 1563 3006 3379 1563 Size: 1443 373 3063 Fragments arranged by size: 3063 1443 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2942 4326 4594 1632 Size: 1310 1384 268 1917 Fragments arranged by size: 1917 1384 1310 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4682 AflIII A'CryG_T Cuts at: 1203 3778 4755 1203 Size: 2575 977 1327 Fragments arranged by size: 2575 1327 977 AgeI A'CCGG_T Cuts at: 3710 3710 Size: 4879 AhdI GACnn_n'nnGTC Cuts at: 3608 3608 Size: 4879 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4879 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4879 AlwNI CAG_nnn'CTG Cuts at: 2622 4194 2622 Size: 1572 3307 ApaLI G'TGCA_C Cuts at: 2936 4092 2936 Size: 1156 3723 ApoI r'AATT_y Cuts at: 1493 2188 2414 2566 4675 1493 Size: 695 226 152 2109 1697 Fragments arranged by size: 2109 1697 695 226 152 AseI AT'TA_AT Cuts at: 160 1032 3433 160 Size: 872 2401 1606 Fragments arranged by size: 2401 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4879 AvaI C'yCGr_G Cuts at: 1870 1943 2175 2204 1870 Size: 73 232 29 4545 Fragments arranged by size: 4545 232 73 29 BamHI G'GATC_C Cuts at: 2098 2209 2576 2098 Size: 111 367 4401 Fragments arranged by size: 4401 367 111 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2200 3655 618 Size: 325 217 627 413 1455 1842 Fragments arranged by size: 1842 1455 627 413 325 217 BanII G_rGCy'C Cuts at: 1391 2198 1391 Size: 807 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 3908 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3070 3104 1903 Size: 34 1133 34 3678 Fragments arranged by size: 3678 1133 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2117 2771 3981 2117 Size: 654 1210 3015 Fragments arranged by size: 3015 1210 654 BclI T'GATC_A Cuts at: 1548 1548 Size: 4879 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3490 136 Size: 107 122 71 631 605 1818 1525 Fragments arranged by size: 1818 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4874 4874 Size: 4879 BlpI GC'TnA_GC Cuts at: 2296 2296 Size: 4879 Bme1580I G_kGCm'C Cuts at: 1790 2940 4096 1790 Size: 1150 1156 2573 Fragments arranged by size: 2573 1156 1150 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4879 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2610 3568 442 Size: 416 53 83 399 24 1193 958 1753 Fragments arranged by size: 1753 1193 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4879 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3539 1239 Size: 2300 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3004 3869 4167 3004 Size: 865 298 3716 Fragments arranged by size: 3716 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3542 882 Size: 617 2043 2219 Fragments arranged by size: 2219 2043 617 BsaAI yAC'GTr Cuts at: 493 1204 2670 493 Size: 711 1466 2702 Fragments arranged by size: 2702 1466 711 BsaBI GATnn'nnATC Cuts at: 2054 4873 2054 Size: 2819 2060 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2686 3068 3703 275 Size: 53 83 186 936 1153 382 635 1451 Fragments arranged by size: 1451 1153 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3312 3710 3984 4131 4542 3312 Size: 398 274 147 411 3649 Fragments arranged by size: 3649 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4654 Fragments arranged by size: 4654 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 4879 BseYI C'CCAG_C Cuts at: 4082 4578 4082 Size: 496 4383 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4306 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2329 3090 4118 1540 Size: 275 186 328 761 1028 2301 Fragments arranged by size: 2301 1028 761 328 275 186 BsiHKAI G_wGCw'C Cuts at: 2940 3025 4096 2940 Size: 85 1071 3723 Fragments arranged by size: 3723 1071 85 BsmI GAATG_Cn' Cuts at: 2390 2483 2390 Size: 93 4786 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2198 2940 3025 4096 1391 Size: 399 408 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 408 399 85 BspEI T'CCGG_A Cuts at: 4542 4542 Size: 4879 BspHI T'CATG_A Cuts at: 2768 4498 2768 Size: 1730 3149 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2234 844 Size: 1390 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2766 1394 Size: 429 943 3507 Fragments arranged by size: 3507 943 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3374 3548 65 Size: 1183 2126 174 1396 Fragments arranged by size: 2126 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3523 3710 1700 Size: 110 1713 187 2869 Fragments arranged by size: 2869 1713 187 110 BsrGI T'GTAC_A Cuts at: 96 2136 96 Size: 2040 2839 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4879 BssSI C'ACGA_G Cuts at: 2939 3951 2939 Size: 1012 3867 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4879 BstBI TT'CG_AA Cuts at: 3683 3683 Size: 4879 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4879 BstYI r'GATC_y Cuts at: 1972 2098 2209 2576 2961 2978 4430 4516 4528 Size: 126 111 367 385 17 1452 86 12 Cuts at: 4528 4874 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 346 126 111 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3388 Fragments arranged by size: 3388 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4140 Fragments arranged by size: 4140 683 56 BtsI GCAGTG_nn' Cuts at: 2395 3189 3209 2395 Size: 794 20 4065 Fragments arranged by size: 4065 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4879 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4844 DraI TTT'AAA Cuts at: 2183 2183 Size: 4879 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3886 809 Size: 507 2570 1802 Fragments arranged by size: 2570 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2326 3215 4844 Size: 54 1425 50 275 18 496 889 1629 Cuts at: 4844 8 Size: 43 Fragments arranged by size: 1629 1425 889 496 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2326 1537 Size: 275 514 4090 Fragments arranged by size: 4090 514 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2809 1353 Size: 1456 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3466 3840 3986 387 Size: 1064 138 1877 374 146 1280 Fragments arranged by size: 1877 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2942 3539 4326 4594 1239 Size: 393 1310 597 787 268 1524 Fragments arranged by size: 1524 1310 787 597 393 268 EcoRI G'AATT_C Cuts at: 2188 2566 2188 Size: 378 4501 FspI TGC'GCA Cuts at: 3385 3385 Size: 4879 HaeII r_GCGC'y Cuts at: 1678 4026 1678 Size: 2348 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3520 3552 3594 3626 1373 Size: 32 135 32 1948 32 42 32 2626 Fragments arranged by size: 2626 1948 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2223 2469 669 Size: 988 281 285 246 3079 Fragments arranged by size: 3079 988 285 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4879 HpaI GTT'AAC Cuts at: 2469 2469 Size: 4879 KpnI G_GTAC'C Cuts at: 2204 2204 Size: 4879 MfeI C'AATT_G Cuts at: 2478 2478 Size: 4879 MluI A'CGCG_T Cuts at: 4755 4755 Size: 4879 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3993 4177 1305 Size: 2688 184 2007 Fragments arranged by size: 2688 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3057 Fragments arranged by size: 3057 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2837 3196 3355 518 Size: 648 1671 359 159 2042 Fragments arranged by size: 2042 1671 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2295 2972 4120 4365 1606 Size: 689 677 1148 245 2120 Fragments arranged by size: 2120 1148 689 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3388 Fragments arranged by size: 3388 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 4879 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4879 NotI GC'GGCC_GC Cuts at: 2326 2326 Size: 4879 NspI r_CATG'y Cuts at: 1132 1229 2237 3782 1132 Size: 97 1008 1545 2229 Fragments arranged by size: 2229 1545 1008 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4879 PciI A'CATG_T Cuts at: 3778 3778 Size: 4879 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4879 PmeI GTTT'AAAC Cuts at: 2183 2183 Size: 4879 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2948 2980 4461 4493 2948 Size: 32 1481 32 3334 Fragments arranged by size: 3334 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4879 PsiI TTA'TAA Cuts at: 2449 2449 Size: 4879 PspXI vC'TCGA_Gb Cuts at: 2175 2175 Size: 4879 PstI C_TGCA'G Cuts at: 830 2231 830 Size: 1401 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4879 SalI G'TCGA_C Cuts at: 1936 2221 1936 Size: 285 4594 SbfI CC_TGCA'GG Cuts at: 2231 2231 Size: 4879 ScaI AGT'ACT Cuts at: 3127 4839 3127 Size: 1712 3167 SfcI C'TryA_G Cuts at: 826 1045 2227 3362 4043 4234 826 Size: 219 1182 1135 681 191 1471 Fragments arranged by size: 1471 1182 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4879 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4879 SmaI CCC'GGG Cuts at: 1872 2206 1872 Size: 334 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2175 2983 3884 4146 820 Size: 197 926 232 808 901 262 1553 Fragments arranged by size: 1553 926 901 808 262 232 197 SnaBI TAC'GTA Cuts at: 493 2670 493 Size: 2177 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4879 SphI G_CATG'C Cuts at: 1132 2237 1132 Size: 1105 3774 SspI AAT'ATT Cuts at: 5 52 2803 5 Size: 47 2751 2081 Fragments arranged by size: 2751 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4879 StyI C'CwwG_G Cuts at: 513 1065 2004 2318 513 Size: 552 939 314 3074 Fragments arranged by size: 3074 939 552 314 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2027 2906 2923 3076 3261 3706 325 Size: 1255 447 879 17 153 185 445 1498 Fragments arranged by size: 1498 1255 879 447 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2136 3125 4837 Size: 274 80 33 51 1443 159 989 1712 Cuts at: 4837 96 Size: 138 Fragments arranged by size: 1712 1443 989 274 159 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4839 4871 4839 Size: 32 4847 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4879 XbaI T'CTAG_A Cuts at: 2215 2215 Size: 4879 XhoI C'TCGA_G Cuts at: 1943 2175 1943 Size: 232 4647 XmaI C'CCGG_G Cuts at: 1870 2204 1870 Size: 334 4545 XmnI GAAnn'nnTTC Cuts at: 3008 3008 Size: 4879 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2687 276 Size: 53 83 186 936 1153 2468 Fragments arranged by size: 2468 1153 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI