(Circular) (Six-base) MAPSORT of: pFN21AB7452.seq Check: 7779 from: 1 to: 5518 pFN21AB7452 5518 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2573 2573 Size: 5518 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3328 278 Size: 53 83 186 936 1792 2468 Fragments arranged by size: 2468 1792 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2861 1937 Size: 924 4594 Acc65I G'GTAC_C Cuts at: 2035 2839 2035 Size: 804 4714 AclI AA'CG_TT Cuts at: 1563 3645 4018 1563 Size: 2082 373 3063 Fragments arranged by size: 3063 2082 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2183 3581 4965 5233 1632 Size: 551 1398 1384 268 1917 Fragments arranged by size: 1917 1398 1384 551 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5321 AflIII A'CryG_T Cuts at: 1203 4417 5394 1203 Size: 3214 977 1327 Fragments arranged by size: 3214 1327 977 AgeI A'CCGG_T Cuts at: 4349 4349 Size: 5518 AhdI GACnn_n'nnGTC Cuts at: 4247 4247 Size: 5518 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5518 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5518 AlwNI CAG_nnn'CTG Cuts at: 2278 2296 2617 3261 4833 2278 Size: 18 321 644 1572 2963 Fragments arranged by size: 2963 1572 644 321 18 ApaLI G'TGCA_C Cuts at: 3575 4731 3575 Size: 1156 4362 ApoI r'AATT_y Cuts at: 1493 2827 3053 3205 5314 1493 Size: 1334 226 152 2109 1697 Fragments arranged by size: 2109 1697 1334 226 152 AseI AT'TA_AT Cuts at: 160 1032 4072 160 Size: 872 3040 1606 Fragments arranged by size: 3040 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5518 AvaI C'yCGr_G Cuts at: 1870 1943 2082 2183 2266 2474 2843 1870 Size: 73 139 101 83 208 369 4545 Fragments arranged by size: 4545 369 208 139 101 83 73 BamHI G'GATC_C Cuts at: 2848 3215 2848 Size: 367 5151 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2035 2252 2313 2488 2530 Size: 325 217 627 248 217 61 175 42 Cuts at: 2530 2746 2839 4294 618 Size: 216 93 1455 1842 Fragments arranged by size: 1842 1455 627 325 248 217 217 216 175 93 61 42 BanII G_rGCy'C Cuts at: 1391 2265 2837 1391 Size: 874 572 4072 Fragments arranged by size: 4072 874 572 BbeI G_GCGC'C Cuts at: 2256 2492 2750 2256 Size: 236 258 5024 Fragments arranged by size: 5024 258 236 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2536 928 Size: 971 637 3910 Fragments arranged by size: 3910 971 637 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3709 3743 1903 Size: 34 1772 34 3678 Fragments arranged by size: 3678 1772 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2089 3410 4620 2089 Size: 1321 1210 2987 Fragments arranged by size: 2987 1321 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5518 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2204 2463 Size: 107 122 71 631 605 337 195 259 Cuts at: 2463 2639 2655 4129 136 Size: 176 16 1474 1525 Fragments arranged by size: 1525 1474 631 605 337 259 195 176 122 107 71 16 BglII A'GATC_T Cuts at: 5513 5513 Size: 5518 BlpI GC'TnA_GC Cuts at: 2537 2935 2537 Size: 398 5120 Bme1580I G_kGCm'C Cuts at: 1790 2558 3579 4735 1790 Size: 768 1021 1156 2573 Fragments arranged by size: 2573 1156 1021 768 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5518 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3249 4207 442 Size: 416 53 83 399 24 1832 958 1753 Fragments arranged by size: 1832 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5518 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2752 2752 Size: 5518 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2192 2600 2639 2699 2747 2792 4178 1239 Size: 953 408 39 60 48 45 1386 2579 Fragments arranged by size: 2579 1386 953 408 60 48 45 39 Bpu10I CC'TnA_GC Cuts at: 2384 2444 2384 Size: 60 5458 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3643 4508 4806 3643 Size: 865 298 4355 Fragments arranged by size: 4355 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4181 882 Size: 617 2682 2219 Fragments arranged by size: 2682 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2150 3309 493 Size: 711 946 1159 2702 Fragments arranged by size: 2702 1159 946 711 BsaBI GATnn'nnATC Cuts at: 5512 5512 Size: 5518 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2253 2489 2703 2747 Size: 53 83 186 936 720 236 214 44 Cuts at: 2747 3325 3707 4342 275 Size: 578 382 635 1451 Fragments arranged by size: 1451 936 720 635 578 382 236 214 186 83 53 44 BsaWI w'CCGG_w Cuts at: 3951 4349 4623 4770 5181 3951 Size: 398 274 147 411 4288 Fragments arranged by size: 4288 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5293 Fragments arranged by size: 5293 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2123 2201 2492 2777 1186 Size: 937 78 291 285 3927 Fragments arranged by size: 3927 937 291 285 78 BseYI C'CCAG_C Cuts at: 4721 5217 4721 Size: 496 5022 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2528 1163 Size: 573 792 4153 Fragments arranged by size: 4153 792 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2049 2068 2100 2196 2631 2647 Size: 275 186 48 19 32 96 435 16 Cuts at: 2647 2968 3729 4757 1540 Size: 321 761 1028 2301 Fragments arranged by size: 2301 1028 761 435 321 275 186 96 48 32 19 16 BsiHKAI G_wGCw'C Cuts at: 3579 3664 4735 3579 Size: 85 1071 4362 Fragments arranged by size: 4362 1071 85 BsmI GAATG_Cn' Cuts at: 3029 3122 3029 Size: 93 5425 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2265 2558 2837 3579 3664 4735 1391 Size: 399 475 293 279 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 475 399 293 279 85 BspEI T'CCGG_A Cuts at: 5181 5181 Size: 5518 BspHI T'CATG_A Cuts at: 3407 5137 3407 Size: 1730 3788 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2116 2573 2873 844 Size: 1272 457 300 3489 Fragments arranged by size: 3489 1272 457 300 BsrBI CCG'CTC Cuts at: 1394 1823 3405 1394 Size: 429 1582 3507 Fragments arranged by size: 3507 1582 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4013 4187 65 Size: 1183 2765 174 1396 Fragments arranged by size: 2765 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2044 2048 2390 2597 2732 2809 4162 Size: 110 234 4 342 207 135 77 1353 Cuts at: 4162 4349 1700 Size: 187 2869 Fragments arranged by size: 2869 1353 342 234 207 187 135 110 77 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5518 BssHII G'CGCG_C Cuts at: 1925 2304 2591 2593 2601 2685 2717 1925 Size: 379 287 2 8 84 32 4726 Fragments arranged by size: 4726 379 287 84 32 8 2 BssSI C'ACGA_G Cuts at: 2522 3578 4590 2522 Size: 1056 1012 3450 Fragments arranged by size: 3450 1056 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5518 BstBI TT'CG_AA Cuts at: 4322 4322 Size: 5518 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5518 BstYI r'GATC_y Cuts at: 1972 2848 3215 3600 3617 5069 5155 5167 5513 Size: 876 367 385 17 1452 86 12 346 Cuts at: 5513 1972 Size: 1977 Fragments arranged by size: 1977 1452 876 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2637 513 Size: 552 939 633 3394 Fragments arranged by size: 3394 939 633 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2779 487 Size: 683 56 1553 3226 Fragments arranged by size: 3226 1553 683 56 BtsI GCAGTG_nn' Cuts at: 2019 2516 3034 3828 3848 2019 Size: 497 518 794 20 3689 Fragments arranged by size: 3689 794 518 497 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5518 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5483 DraI TTT'AAA Cuts at: 2822 2822 Size: 5518 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4525 809 Size: 507 3209 1802 Fragments arranged by size: 3209 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2065 2097 Size: 54 1425 50 275 18 216 19 32 Cuts at: 2097 2464 2628 2640 2965 3854 5483 8 Size: 367 164 12 325 889 1629 43 Fragments arranged by size: 1629 1425 889 367 325 275 216 164 54 50 43 32 19 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2046 2065 2097 2628 2965 1537 Size: 275 234 19 32 531 337 4090 Fragments arranged by size: 4090 531 337 275 234 32 19 EarI CTCTTCn'nnn_ Cuts at: 1353 2399 2612 2795 3448 1353 Size: 1046 213 183 653 3423 Fragments arranged by size: 3423 1046 653 213 183 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4105 4479 4625 387 Size: 1064 138 2516 374 146 1280 Fragments arranged by size: 2516 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2183 2192 2600 2639 2699 2747 2792 Size: 393 551 9 408 39 60 48 45 Cuts at: 2792 3581 4178 4965 5233 1239 Size: 789 597 787 268 1524 Fragments arranged by size: 1524 789 787 597 551 408 393 268 60 48 45 39 9 EcoNI CCTnn'n_nnAGG Cuts at: 2123 2162 2123 Size: 39 5479 EcoRI G'AATT_C Cuts at: 2827 3205 2827 Size: 378 5140 FspI TGC'GCA Cuts at: 2501 4024 2501 Size: 1523 3995 HaeII r_GCGC'y Cuts at: 1678 2256 2296 2391 2492 2750 4665 1678 Size: 578 40 95 101 258 1915 2531 Fragments arranged by size: 2531 1915 578 258 101 95 40 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4159 4191 4233 4265 1373 Size: 32 135 32 2587 32 42 32 2626 Fragments arranged by size: 2626 2587 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2862 3108 669 Size: 988 281 924 246 3079 Fragments arranged by size: 3079 988 924 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5518 HpaI GTT'AAC Cuts at: 3108 3108 Size: 5518 KasI G'GCGC_C Cuts at: 2252 2488 2746 2252 Size: 236 258 5024 Fragments arranged by size: 5024 258 236 KpnI G_GTAC'C Cuts at: 2039 2843 2039 Size: 804 4714 MfeI C'AATT_G Cuts at: 3117 3117 Size: 5518 MluI A'CGCG_T Cuts at: 5394 5394 Size: 5518 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4632 4816 1305 Size: 3327 184 2007 Fragments arranged by size: 3327 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3696 Fragments arranged by size: 3696 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3476 3835 3994 518 Size: 648 2310 359 159 2042 Fragments arranged by size: 2310 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2064 2494 2536 2614 2934 3611 4759 5004 Size: 458 430 42 78 320 677 1148 245 Cuts at: 5004 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 458 430 320 245 78 42 NaeI GCC'GGC Cuts at: 2046 2392 2599 2734 2046 Size: 346 207 135 4830 Fragments arranged by size: 4830 346 207 135 NarI GG'CG_CC Cuts at: 2253 2489 2747 2253 Size: 236 258 5024 Fragments arranged by size: 5024 258 236 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4027 Fragments arranged by size: 4027 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5518 NgoMIV G'CCGG_C Cuts at: 2044 2390 2597 2732 2044 Size: 346 207 135 4830 Fragments arranged by size: 4830 346 207 135 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5518 NotI GC'GGCC_GC Cuts at: 2065 2965 2065 Size: 900 4618 NruI TCG'CGA Cuts at: 2798 2798 Size: 5518 NspI r_CATG'y Cuts at: 1132 1229 2876 4421 1132 Size: 97 1647 1545 2229 Fragments arranged by size: 2229 1647 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5518 PciI A'CATG_T Cuts at: 4417 4417 Size: 5518 PflMI CCAn_nnn'nTGG Cuts at: 1108 2617 1108 Size: 1509 4009 PfoI T'CCnGG_A Cuts at: 2724 2724 Size: 5518 PmeI GTTT'AAAC Cuts at: 2822 2822 Size: 5518 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3587 3619 5100 5132 3587 Size: 32 1481 32 3973 Fragments arranged by size: 3973 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5518 PsiI TTA'TAA Cuts at: 3088 3088 Size: 5518 PstI C_TGCA'G Cuts at: 830 2286 2694 2870 830 Size: 1456 408 176 3478 Fragments arranged by size: 3478 1456 408 176 PvuI CG_AT'CG Cuts at: 2001 2196 2001 Size: 195 5323 PvuII CAG'CTG Cuts at: 2614 2614 Size: 5518 SalI G'TCGA_C Cuts at: 1936 2860 1936 Size: 924 4594 SapI GCTCTTCn'nnn_ Cuts at: 2612 2795 2612 Size: 183 5335 SbfI CC_TGCA'GG Cuts at: 2870 2870 Size: 5518 ScaI AGT'ACT Cuts at: 3766 5478 3766 Size: 1712 3806 SfcI C'TryA_G Cuts at: 826 1045 2282 2431 2690 2866 4001 4682 4873 Size: 219 1237 149 259 176 1135 681 191 Cuts at: 4873 826 Size: 1471 Fragments arranged by size: 1471 1237 1135 681 259 219 191 176 149 SfoI GGC'GCC Cuts at: 2254 2490 2748 2254 Size: 236 258 5024 Fragments arranged by size: 5024 258 236 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5518 SgrAI Cr'CCGG_yG Cuts at: 1700 2390 2597 1700 Size: 690 207 4621 Fragments arranged by size: 4621 690 207 SmaI CCC'GGG Cuts at: 1872 2084 2268 2845 1872 Size: 212 184 577 4545 Fragments arranged by size: 4545 577 212 184 SmlI C'TyrA_G Cuts at: 820 1017 1943 3622 4523 4785 820 Size: 197 926 1679 901 262 1553 Fragments arranged by size: 1679 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3309 493 Size: 2816 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5518 SphI G_CATG'C Cuts at: 1132 2876 1132 Size: 1744 3774 SspI AAT'ATT Cuts at: 5 52 3442 5 Size: 47 3390 2081 Fragments arranged by size: 3390 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5518 StyI C'CwwG_G Cuts at: 513 1065 2004 2957 513 Size: 552 939 953 3074 Fragments arranged by size: 3074 953 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3545 3562 3715 3900 4345 325 Size: 1255 1965 17 153 185 445 1498 Fragments arranged by size: 1965 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3764 5476 96 Size: 274 80 33 51 1443 1787 1712 138 Fragments arranged by size: 1787 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5478 5510 5478 Size: 32 5486 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5518 XbaI T'CTAG_A Cuts at: 2854 2854 Size: 5518 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5518 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5518 XmaI C'CCGG_G Cuts at: 1870 2082 2266 2843 1870 Size: 212 184 577 4545 Fragments arranged by size: 4545 577 212 184 XmnI GAAnn'nnTTC Cuts at: 3647 3647 Size: 5518 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3326 276 Size: 53 83 186 936 1792 2468 Fragments arranged by size: 2468 1792 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI