(Circular) (Six-base) MAPSORT of: pFN21AB7447.seq Check: 6970 from: 1 to: 5521 pFN21AB7447 5521 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3331 278 Size: 53 83 186 936 1795 2468 Fragments arranged by size: 2468 1795 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2864 1937 Size: 927 4594 Acc65I G'GTAC_C Cuts at: 2842 2842 Size: 5521 AclI AA'CG_TT Cuts at: 1563 3648 4021 1563 Size: 2085 373 3063 Fragments arranged by size: 3063 2085 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2660 2826 3584 4968 5236 1632 Size: 1028 166 758 1384 268 1917 Fragments arranged by size: 1917 1384 1028 758 268 166 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5324 AflIII A'CryG_T Cuts at: 1203 4420 5397 1203 Size: 3217 977 1327 Fragments arranged by size: 3217 1327 977 AgeI A'CCGG_T Cuts at: 4352 4352 Size: 5521 AhdI GACnn_n'nnGTC Cuts at: 4250 4250 Size: 5521 AleI CACnn'nnGTG Cuts at: 1166 2456 1166 Size: 1290 4231 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5521 AlwNI CAG_nnn'CTG Cuts at: 2199 2684 3264 4836 2199 Size: 485 580 1572 2884 Fragments arranged by size: 2884 1572 580 485 ApaLI G'TGCA_C Cuts at: 3578 4734 3578 Size: 1156 4365 ApoI r'AATT_y Cuts at: 1493 2773 2830 3056 3208 5317 1493 Size: 1280 57 226 152 2109 1697 Fragments arranged by size: 2109 1697 1280 226 152 57 AseI AT'TA_AT Cuts at: 160 1032 4075 160 Size: 872 3043 1606 Fragments arranged by size: 3043 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5521 AvaI C'yCGr_G Cuts at: 1870 1943 2846 1870 Size: 73 903 4545 Fragments arranged by size: 4545 903 73 BamHI G'GATC_C Cuts at: 2851 3218 2851 Size: 367 5154 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2842 4297 618 Size: 325 217 627 1055 1455 1842 Fragments arranged by size: 1842 1455 1055 627 325 217 BanII G_rGCy'C Cuts at: 1391 2228 2306 2328 2840 1391 Size: 837 78 22 512 4072 Fragments arranged by size: 4072 837 512 78 22 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2584 928 Size: 971 685 3865 Fragments arranged by size: 3865 971 685 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3712 3746 1903 Size: 34 1775 34 3678 Fragments arranged by size: 3678 1775 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3413 4623 3413 Size: 1210 4311 BclI T'GATC_A Cuts at: 1548 1548 Size: 5521 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4132 136 Size: 107 122 71 631 605 2460 1525 Fragments arranged by size: 2460 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5516 5516 Size: 5521 BlpI GC'TnA_GC Cuts at: 2938 2938 Size: 5521 Bme1580I G_kGCm'C Cuts at: 1790 3582 4738 1790 Size: 1792 1156 2573 Fragments arranged by size: 2573 1792 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5521 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2262 3252 4210 Size: 416 53 83 399 24 845 990 958 Cuts at: 4210 442 Size: 1753 Fragments arranged by size: 1753 990 958 845 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5521 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2096 2717 4181 1239 Size: 857 621 1464 2579 Fragments arranged by size: 2579 1464 857 621 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3646 4511 4809 3646 Size: 865 298 4358 Fragments arranged by size: 4358 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2345 4184 882 Size: 617 846 1839 2219 Fragments arranged by size: 2219 1839 846 617 BsaAI yAC'GTr Cuts at: 493 1204 2440 2602 3312 493 Size: 711 1236 162 710 2702 Fragments arranged by size: 2702 1236 711 710 162 BsaBI GATnn'nnATC Cuts at: 5515 5515 Size: 5521 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3328 3710 4345 275 Size: 53 83 186 936 1795 382 635 1451 Fragments arranged by size: 1795 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2370 2487 3954 4352 4626 4773 5184 2370 Size: 117 1467 398 274 147 411 2707 Fragments arranged by size: 2707 1467 411 398 274 147 117 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2574 2604 1178 Size: 30 165 30 1171 30 4095 Fragments arranged by size: 4095 1171 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5521 BseYI C'CCAG_C Cuts at: 4724 5220 4724 Size: 496 5025 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2233 1163 Size: 573 497 4451 Fragments arranged by size: 4451 573 497 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2971 3732 4760 1540 Size: 275 186 970 761 1028 2301 Fragments arranged by size: 2301 1028 970 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2306 2328 3582 3667 4738 2306 Size: 22 1254 85 1071 3089 Fragments arranged by size: 3089 1254 1071 85 22 BsmI GAATG_Cn' Cuts at: 3032 3125 3032 Size: 93 5428 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2228 2306 2328 2840 3582 3667 4738 Size: 399 438 78 22 512 742 85 1071 Cuts at: 4738 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 512 438 399 85 78 22 BspEI T'CCGG_A Cuts at: 2370 5184 2370 Size: 2814 2707 BspHI T'CATG_A Cuts at: 3410 5140 3410 Size: 1730 3791 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2876 844 Size: 2032 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3408 1394 Size: 429 1585 3507 Fragments arranged by size: 3507 1585 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2521 4016 4190 65 Size: 1183 1273 1495 174 1396 Fragments arranged by size: 1495 1396 1273 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4165 4352 1700 Size: 110 2355 187 2869 Fragments arranged by size: 2869 2355 187 110 BsrGI T'GTAC_A Cuts at: 96 2665 96 Size: 2569 2952 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5521 BssSI C'ACGA_G Cuts at: 3581 4593 3581 Size: 1012 4509 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5521 BstBI TT'CG_AA Cuts at: 4325 4325 Size: 5521 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5521 BstXI CCAn_nnnn'nTGG Cuts at: 2695 2695 Size: 5521 BstYI r'GATC_y Cuts at: 1972 2851 3218 3603 3620 5072 5158 5170 5516 Size: 879 367 385 17 1452 86 12 346 Cuts at: 5516 1972 Size: 1977 Fragments arranged by size: 1977 1452 879 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4969 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4782 Fragments arranged by size: 4782 683 56 BtsI GCAGTG_nn' Cuts at: 2203 2258 2682 2777 3037 3831 3851 2203 Size: 55 424 95 260 794 20 3873 Fragments arranged by size: 3873 794 424 260 95 55 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5521 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2006 2041 529 Size: 35 1442 35 4009 Fragments arranged by size: 4009 1442 35 35 DraI TTT'AAA Cuts at: 2037 2825 2037 Size: 788 4733 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2525 4528 809 Size: 507 1209 2003 1802 Fragments arranged by size: 2003 1802 1209 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2537 2968 3857 Size: 54 1425 50 275 18 707 431 889 Cuts at: 3857 5486 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 707 431 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2968 1537 Size: 275 1156 4090 Fragments arranged by size: 4090 1156 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2214 3451 1353 Size: 861 1237 3423 Fragments arranged by size: 3423 1237 861 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4108 4482 4628 387 Size: 1064 138 2519 374 146 1280 Fragments arranged by size: 2519 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2304 2326 2304 Size: 22 5499 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2096 2660 2717 2826 3584 4181 4968 Size: 393 464 564 57 109 758 597 787 Cuts at: 4968 5236 1239 Size: 268 1524 Fragments arranged by size: 1524 787 758 597 564 464 393 268 109 57 EcoRI G'AATT_C Cuts at: 2830 3208 2830 Size: 378 5143 EcoRV GAT'ATC Cuts at: 2061 2061 Size: 5521 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2356 2356 Size: 5521 FspI TGC'GCA Cuts at: 4027 4027 Size: 5521 HaeII r_GCGC'y Cuts at: 1678 4668 1678 Size: 2990 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2574 2606 4162 4194 4236 Size: 32 135 32 1002 32 1556 32 42 Cuts at: 4236 4268 1373 Size: 32 2626 Fragments arranged by size: 2626 1556 1002 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2865 3111 669 Size: 988 281 927 246 3079 Fragments arranged by size: 3079 988 927 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5521 HpaI GTT'AAC Cuts at: 3111 3111 Size: 5521 KpnI G_GTAC'C Cuts at: 2846 2846 Size: 5521 MfeI C'AATT_G Cuts at: 3120 3120 Size: 5521 MluI A'CGCG_T Cuts at: 5397 5397 Size: 5521 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4635 4819 1305 Size: 3330 184 2007 Fragments arranged by size: 3330 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2539 10 Size: 54 1425 343 707 2992 Fragments arranged by size: 2992 1425 707 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2456 3479 3838 3997 518 Size: 648 1290 1023 359 159 2042 Fragments arranged by size: 2042 1290 1023 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2495 2937 3614 4762 5007 1606 Size: 889 442 677 1148 245 2120 Fragments arranged by size: 2120 1148 889 677 442 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4969 NdeI CA'TA_TG Cuts at: 387 387 Size: 5521 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5521 NotI GC'GGCC_GC Cuts at: 2968 2968 Size: 5521 NruI TCG'CGA Cuts at: 2447 2447 Size: 5521 NspI r_CATG'y Cuts at: 1132 1229 2879 4424 1132 Size: 97 1650 1545 2229 Fragments arranged by size: 2229 1650 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5521 PciI A'CATG_T Cuts at: 4420 4420 Size: 5521 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5521 PmeI GTTT'AAAC Cuts at: 2825 2825 Size: 5521 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3590 3622 5103 5135 3590 Size: 32 1481 32 3976 Fragments arranged by size: 3976 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5521 PsiI TTA'TAA Cuts at: 3091 3091 Size: 5521 PstI C_TGCA'G Cuts at: 830 2195 2791 2873 830 Size: 1365 596 82 3478 Fragments arranged by size: 3478 1365 596 82 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5521 SacI G_AGCT'C Cuts at: 2306 2328 2306 Size: 22 5499 SalI G'TCGA_C Cuts at: 1936 2863 1936 Size: 927 4594 SbfI CC_TGCA'GG Cuts at: 2873 2873 Size: 5521 ScaI AGT'ACT Cuts at: 3769 5481 3769 Size: 1712 3809 SfcI C'TryA_G Cuts at: 826 1045 2191 2387 2787 2869 4004 4685 4876 Size: 219 1146 196 400 82 1135 681 191 Cuts at: 4876 826 Size: 1471 Fragments arranged by size: 1471 1146 1135 681 400 219 196 191 82 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5521 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5521 SmaI CCC'GGG Cuts at: 1872 2848 1872 Size: 976 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3625 4526 4788 820 Size: 197 926 1682 901 262 1553 Fragments arranged by size: 1682 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2602 3312 493 Size: 2109 710 2702 Fragments arranged by size: 2702 2109 710 SpeI A'CTAG_T Cuts at: 152 152 Size: 5521 SphI G_CATG'C Cuts at: 1132 2879 1132 Size: 1747 3774 SspI AAT'ATT Cuts at: 5 52 2418 3445 5 Size: 47 2366 1027 2081 Fragments arranged by size: 2366 2081 1027 47 StuI AGG'CCT Cuts at: 1517 2645 1517 Size: 1128 4393 StyI C'CwwG_G Cuts at: 513 1065 2960 513 Size: 552 1895 3074 Fragments arranged by size: 3074 1895 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3548 3565 3718 3903 4348 325 Size: 1255 1968 17 153 185 445 1498 Fragments arranged by size: 1968 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2665 3767 5479 Size: 274 80 33 51 1443 688 1102 1712 Cuts at: 5479 96 Size: 138 Fragments arranged by size: 1712 1443 1102 688 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5481 5513 5481 Size: 32 5489 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5521 XbaI T'CTAG_A Cuts at: 2857 2857 Size: 5521 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5521 XmaI C'CCGG_G Cuts at: 1870 2846 1870 Size: 976 4545 XmnI GAAnn'nnTTC Cuts at: 2417 2705 3650 2417 Size: 288 945 4288 Fragments arranged by size: 4288 945 288 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3329 276 Size: 53 83 186 936 1795 2468 Fragments arranged by size: 2468 1795 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI