Restriction map for
pFN21AB7428
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BciVI BclI BfrI BglII BlnI
BpuAI BsaAI BsaBI BsaI BseAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstYI
BstZ17I Cfr10I ClaI CpoI DraI
DraIII DrdI Eam1105I EcoNI EcoRV
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MslI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI TfiI XbaI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 1129 GTCTAC GT^[AC][GT]AC
AcyI 393 GACGCC G[GA]^CG[CT]C
Aor51HI 615 AGCGCT AGC^GCT
AspI 774 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 939 GGCACC G^G[CT][GA]CC
BglI 763 GCCCCTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 637 CTCCAG CTGGAG(16/14)
BsaHI 393 GACGCC G[GA]^CG[CT]C
BsaWI 104 ACCGGA [AT]^CCGG[AT]
BsgI 1193 CTGCAC GTGCAG(16/14)
BsiEI 551 CGGCCG CG[GA][CT]^CG
BsiWI 596 CGTACG C^GTACG
BsmAI 607 GAGAC GTCTC(1/5)
BspMI 426 GCAGGT ACCTGC(4/8)
BssSI 286 CACGAG CACGAG(-5/-1)
BstEII 459 GGTGACC G^GT[ACGT]ACC
BstPI 459 GGTGACC G^GT[ACGT]ACC
BstXI 264 CCAGCCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 1116 GATC ^GATC
DsaI 1026 CCGTGG C^C[GA][CT]GG
EaeI 551 CGGCCG [CT]^GGCC[GA]
EagI 551 CGGCCG C^GGCCG
EclXI 551 CGGCCG C^GGCCG
Eco47III 615 AGCGCT AGC^GCT
Eco52I 551 CGGCCG C^GGCCG
EcoO65I 459 GGTGACC G^GT[ACGT]ACC
EcoRI 875 GAATTC G^AATTC
EcoT22I 827 ATGCAT ATGCA^T
HgaI 393 GACGC GACGC(5/10)
Hin1I 393 GACGCC G[GA]^CG[CT]C
MboI 1116 GATC ^GATC
NdeII 1116 GATC ^GATC
NsiI 827 ATGCAT ATGCA^T
PaeR7I 611 CTCGAG C^TCGAG
PflFI 774 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 39 CCAGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 10 CTGCAG CTGCA^G
Sau3AI 1116 GATC ^GATC
SmlI 611 CTCGAG C^T[CT][GA]AG
Tth111I 774 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 39 CCAGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 611 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcsI 352 GAATTT [GA]^AATT[CT]
875 GAATTC
AocI 121 CCTGAGG CC^T[ACGT]AGG
1041 CCTGAGG
ApoI 352 GAATTT [GA]^AATT[CT]
875 GAATTC
AvaI 611 CTCGAG C^[CT]CG[GA]G
725 CTCGGG
BcgI 422 CGAAGCAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
21 GCAGGCAGCTCG
BcnI 197 CCGGG CC^[GC]GG
754 CCCGG
BfaI 260 CTAG C^TAG
1111 CTAG
BlpI 618 GCTCAGC GC^T[ACGT]AGC
835 GCTCAGC
Bpu1102I 618 GCTCAGC GC^T[ACGT]AGC
835 GCTCAGC
BsoBI 611 CTCGAG C^[CT]CG[GA]G
725 CTCGGG
BsrBI 697 CCGCTC CCGCTC(-3/-3)
1051 GAGCGG
Bsu36I 121 CCTGAGG CC^T[ACGT]AGG
1041 CCTGAGG
CelII 618 GCTCAGC GC^T[ACGT]AGC
835 GCTCAGC
Eco81I 121 CCTGAGG CC^T[ACGT]AGG
1041 CCTGAGG
HaeII 615 AGCGCT [GA]GCGC^[CT]
730 GGCGCT
HphI 459 GGTGA GGTGA(8/7)
545 TCACC
MaeI 260 CTAG C^TAG
1111 CTAG
MaeIII 460 GTGAC ^GT[ACGT]AC
514 GTCAC
MseI 857 TTAA T^TAA
1070 TTAA
NciI 197 CCGGG CC^[GC]GG
754 CCCGG
SacI 325 GAGCTC GAGCT^C
634 GAGCTC
SapI 1048 GAAGAGC GCTCTTC(1/4)
699 GCTCTTC
SfcI 10 CTGCAG C^T[GA][CT]AG
112 CTACAG
Tru9I 857 TTAA T^TAA
1070 TTAA
Tsp45I 460 GTGAC ^GT[GC]AC
514 GTCAC
XcmI 467 CCAAGGGCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
777 CCAGTCCAGCCCTGG
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AfaI AluI ApaI
AspHI AvaII BanII BbvCI BbvI
BmyI BsaJI BseRI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DraII EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI Ksp632I MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NlaIII NlaIV NspI PleI PpuMI
PvuII RsaI Sau96I ScrFI SfaNI
SphI StyI TaqI TseI Tsp509I
TspRI TthHB8I