Restriction map for
pFN21AB7428
(Linear) (Six-base) MAPSORT of: KIBB7428.seq Check: 5315 from: 1 to: 1236
KIBB7428.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 417 1236
Size: 417 819
AccI GT'mk_AC
Cuts at: 0 1130 1236
Size: 1130 106
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 1236
Size: 188 1048
AfeI AGC'GCT
Cuts at: 0 617 1236
Size: 617 619
ApaI G_GGCC'C
Cuts at: 0 240 247 1217 1236
Size: 240 7 970 19
Fragments arranged by size:
970 240 19 7
ApoI r'AATT_y
Cuts at: 0 352 875 1236
Size: 352 523 361
Fragments arranged by size:
523 361 352
AvaI C'yCGr_G
Cuts at: 0 611 725 1236
Size: 611 114 511
Fragments arranged by size:
611 511 114
BanI G'GyrC_C
Cuts at: 0 939 1236
Size: 939 297
BanII G_rGCy'C
Cuts at: 0 240 247 329 638 953 1217 1236
Size: 240 7 82 309 315 264 19
Fragments arranged by size:
315 309 264 240 82 19 7
BbvCI CC'TCA_GC
Cuts at: 0 149 218 526 1236
Size: 149 69 308 710
Fragments arranged by size:
710 308 149 69
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 10 44 411 445 1236
Size: 10 34 367 34 791
Fragments arranged by size:
791 367 34 34 10
BfrBI ATG'CAT
Cuts at: 0 829 1236
Size: 829 407
BglI GCCn_nnn'nGGC
Cuts at: 0 769 1236
Size: 769 467
BlpI GC'TnA_GC
Cuts at: 0 619 836 1236
Size: 619 217 400
Fragments arranged by size:
619 400 217
Bme1580I G_kGCm'C
Cuts at: 0 240 247 413 1217 1236
Size: 240 7 166 804 19
Fragments arranged by size:
804 240 166 19 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 567 771 925 1007 1236
Size: 567 204 154 82 229
Fragments arranged by size:
567 229 204 154 82
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 1236
Size: 622 614
Bpu10I CC'TnA_GC
Cuts at: 0 149 218 526 734 1236
Size: 149 69 308 208 502
Fragments arranged by size:
502 308 208 149 69
BsaHI Gr'CG_yC
Cuts at: 0 394 1236
Size: 394 842
BsaWI w'CCGG_w
Cuts at: 0 104 1236
Size: 104 1132
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 678 708 1236
Size: 678 30 528
Fragments arranged by size:
678 528 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 193 220 223 235 880 961 1236
Size: 193 27 3 12 645 81 275
Fragments arranged by size:
645 275 193 81 27 12 3
BseYI C'CCAG_C
Cuts at: 0 247 741 792 1236
Size: 247 494 51 444
Fragments arranged by size:
494 444 247 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1178 1236
Size: 1178 58
BsiEI CG_ry'CG
Cuts at: 0 554 1236
Size: 554 682
BsiHKAI G_wGCw'C
Cuts at: 0 329 434 638 1236
Size: 329 105 204 598
Fragments arranged by size:
598 329 204 105
BsiWI C'GTAC_G
Cuts at: 0 596 1236
Size: 596 640
Bsp1286I G_dGCh'C
Cuts at: 0 240 247 329 413 434 638 953 1217
Size: 240 7 82 84 21 204 315 264
Cuts at: 1217 1236
Size: 19
Fragments arranged by size:
315 264 240 204 84 82 21 19
7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 417 1236
Size: 417 819
BsrBI CCG'CTC
Cuts at: 0 699 1053 1236
Size: 699 354 183
Fragments arranged by size:
699 354 183
BssSI C'ACGA_G
Cuts at: 0 286 1236
Size: 286 950
BstEII G'GTnAC_C
Cuts at: 0 459 1236
Size: 459 777
BstXI CCAn_nnnn'nTGG
Cuts at: 0 271 1236
Size: 271 965
Bsu36I CC'TnA_GG
Cuts at: 0 122 1042 1236
Size: 122 920 194
Fragments arranged by size:
920 194 122
BtgI C'CryG_G
Cuts at: 0 1026 1236
Size: 1026 210
BtsI GCAGTG_nn'
Cuts at: 0 895 1089 1236
Size: 895 194 147
Fragments arranged by size:
895 194 147
EaeI y'GGCC_r
Cuts at: 0 551 1236
Size: 551 685
EagI C'GGCC_G
Cuts at: 0 551 1236
Size: 551 685
EarI CTCTTCn'nnn_
Cuts at: 0 149 706 955 1043 1236
Size: 149 557 249 88 193
Fragments arranged by size:
557 249 193 149 88
EcoICRI GAG'CTC
Cuts at: 0 327 636 1236
Size: 327 309 600
Fragments arranged by size:
600 327 309
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 622 1236
Size: 188 434 614
Fragments arranged by size:
614 434 188
EcoO109I rG'GnC_Cy
Cuts at: 0 6 117 128 236 243 244 738 751
Size: 6 111 11 108 7 1 494 13
Cuts at: 751 919 979 1213 1214 1236
Size: 168 60 234 1 22
Fragments arranged by size:
494 234 168 111 108 60 22 13
11 7 6 1 1
EcoRI G'AATT_C
Cuts at: 0 875 1236
Size: 875 361
HaeII r_GCGC'y
Cuts at: 0 619 734 1236
Size: 619 115 502
Fragments arranged by size:
619 502 115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1207 1236
Size: 1207 29
MspA1I CmG'CkG
Cuts at: 0 72 217 318 840 1236
Size: 72 145 101 522 396
Fragments arranged by size:
522 396 145 101 72
NsiI A_TGCA'T
Cuts at: 0 831 1236
Size: 831 405
NspI r_CATG'y
Cuts at: 0 68 829 833 1008 1236
Size: 68 761 4 175 228
Fragments arranged by size:
761 228 175 68 4
PasI CC'CwG_GG
Cuts at: 0 39 240 628 798 1176 1236
Size: 39 201 388 170 378 60
Fragments arranged by size:
388 378 201 170 60 39
PflMI CCAn_nnn'nTGG
Cuts at: 0 45 1236
Size: 45 1191
PpuMI rG'GwC_Cy
Cuts at: 0 738 751 919 1236
Size: 738 13 168 317
Fragments arranged by size:
738 317 168 13
PspOMI G'GGCC_C
Cuts at: 0 236 243 1213 1236
Size: 236 7 970 23
Fragments arranged by size:
970 236 23 7
PspXI vC'TCGA_Gb
Cuts at: 0 611 1236
Size: 611 625
PstI C_TGCA'G
Cuts at: 0 14 1236
Size: 14 1222
PvuII CAG'CTG
Cuts at: 0 217 318 840 1236
Size: 217 101 522 396
Fragments arranged by size:
522 396 217 101
SacI G_AGCT'C
Cuts at: 0 329 638 1236
Size: 329 309 598
Fragments arranged by size:
598 329 309
SanDI GG'GwC_CC
Cuts at: 0 751 1236
Size: 751 485
SapI GCTCTTCn'nnn_
Cuts at: 0 706 1043 1236
Size: 706 337 193
Fragments arranged by size:
706 337 193
SfcI C'TryA_G
Cuts at: 0 10 112 1236
Size: 10 102 1124
Fragments arranged by size:
1124 102 10
SmlI C'TyrA_G
Cuts at: 0 611 1236
Size: 611 625
SphI G_CATG'C
Cuts at: 0 68 829 833 1008 1236
Size: 68 761 4 175 228
Fragments arranged by size:
761 228 175 68 4
StyI C'CwwG_G
Cuts at: 0 131 467 746 1236
Size: 131 336 279 490
Fragments arranged by size:
490 336 279 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 586 1236
Size: 586 650
TatI w'GTAC_w
Cuts at: 0 575 1236
Size: 575 661
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 678 710 1236
Size: 678 32 526
Fragments arranged by size:
678 526 32
Tth111I GACn'n_nGTC
Cuts at: 0 777 1236
Size: 777 459
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 474 784 1236
Size: 474 310 452
Fragments arranged by size:
474 452 310
XhoI C'TCGA_G
Cuts at: 0 611 1236
Size: 611 625
Enzymes that do cut:
AarI AccI AcuI AfeI ApaI ApoI AvaI BanI
BanII BbvCI BcgI BfrBI BglI BlpI Bme1580I BmrI
BpmI Bpu10I BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI Bsp1286I BspMI BsrBI BssSI BstEII
BstXI Bsu36I BtgI BtsI EaeI EagI EarI EcoICRI
Eco57MI EcoO109I EcoRI HaeII MmeI MspA1I NsiI NspI
PasI PflMI PpuMI PspOMI PspXI PstI PvuII SacI
SanDI SapI SfcI SmlI SphI StyI TaqII TatI
TstI Tth111I XcmI XhoI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BciVI BclI BglII BmgBI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsmI BsmBI
BspEI BspHI BsrDI BsrFI BsrGI BssHII BstAPI BstBI
BstYI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI
EciI EcoNI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MscI
MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI PacI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PsrI PvuI RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI StuI SwaI XbaI XmaI XmnI ZraI