Restriction map for
pFN21AB7416
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# NO CUT ENZYMES ( 102 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BbrPI BbsI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaI BsaWI BseAI
BsiWI BspDI BspEI BspMI BssHII
Bst1107I BstAPI BstBI BstZ17I CelII
ClaI CpoI DraI Eam1105I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI KspI MaeI
MamI MfeI MroI MseI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspI StuI SwaI
TfiI Tru9I Tsp509I Tth111I XbaI
XcmI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 166 GACGTC GACGT^C
AocI 342 CCTGAGG CC^T[ACGT]AGG
Aor51HI 705 AGCGCT AGC^GCT
BalI 380 TGGCCA TGG^CCA
BbeI 226 GGCGCC GGCGC^C
BcgI 161 GCAGCGACGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1246 GCCCTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1302 TACGTG [CT]AC^GT[GA]
BsiEI 27 CGGCCG CG[GA][CT]^CG
BsmI 852 GAATGC GAATGC(1/-1)
Bsp1407I 1238 TGTACA T^GTACA
BspLU11I 1235 ACATGT A^CATGT
BsrBI 67 GAGCGG CCGCTC(-3/-3)
BsrDI 543 CATTGC GCAATG(2/0)
BsrGI 1238 TGTACA T^GTACA
BssSI 790 CTCGTG CACGAG(-5/-1)
BstEII 132 GGTCACC G^GT[ACGT]ACC
BstPI 132 GGTCACC G^GT[ACGT]ACC
Bsu36I 342 CCTGAGG CC^T[ACGT]AGG
DraIII 148 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 532 GACAGGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 27 CGGCCG C^GGCCG
EarI 1281 GAAGAG CTCTTC(1/4)
EclXI 27 CGGCCG C^GGCCG
Eco47III 705 AGCGCT AGC^GCT
Eco52I 27 CGGCCG C^GGCCG
Eco81I 342 CCTGAGG CC^T[ACGT]AGG
EcoO65I 132 GGTCACC G^GT[ACGT]ACC
HincII 1099 GTCAAC GT[CT]^[GA]AC
HindII 1099 GTCAAC GT[CT]^[GA]AC
HinfI 1207 GACTC G^A[ACGT]TC
KasI 226 GGCGCC G^GCGCC
Ksp632I 1281 GAAGAG CTCTTC(1/4)
MluI 1287 ACGCGT A^CGCGT
MluNI 380 TGGCCA TGG^CCA
MscI 380 TGGCCA TGG^CCA
NarI 226 GGCGCC GG^CGCC
NcoI 1118 CCATGG C^CATGG
PaeR7I 1320 CTCGAG C^TCGAG
PflMI 945 CCACTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1207 GACTC GAGTC(4/5)
PstI 353 CTGCAG CTGCA^G
SacI 916 GAGCTC GAGCT^C
ScaI 692 AGTACT AGT^ACT
SexAI 473 ACCTGGT A^CC[AT]GGT
SfoI 226 GGCGCC GGC^GCC
SphI 83 GCATGC GCATG^C
SspBI 1238 TGTACA T^GTACA
Van91I 945 CCACTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1320 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 1235 ACATGT A^C[GA][CT]GT
1287 ACGCGT
ApaI 593 GGGCCC GGGCC^C
941 GGGCCC
AspHI 649 GTGCTC G[AT]GC[AT]^C
916 GAGCTC
BamHI 628 GGATCC G^GATCC
1265 GGATCC
BanI 226 GGCGCC G^G[CT][GA]CC
800 GGTGCC
BbvCI 987 CCTCAGC CCTCAGC(-5/-2)
501 GCTGAGG
BsiHKAI 649 GTGCTC G[AT]GC[AT]^C
916 GAGCTC
EcoNI 1211 CCTACCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1251 CCTGGCAGAGG
EcoT14I 1118 CCATGG C^C[AT][AT]GG
1216 CCAAGG
MaeII 167 ACGT A^CGT
1303 ACGT
MaeIII 133 GTCAC ^GT[ACGT]AC
154 GTGAC
NaeI 7 GCCGGC GCC^GGC
348 GCCGGC
NgoMIV 7 GCCGGC G^CCGGC
348 GCCGGC
PvuII 499 CAGCTG CAG^CTG
1177 CAGCTG
SfcI 306 CTACAG C^T[GA][CT]AG
353 CTGCAG
SmaI 35 CCCGGG CCC^GGG
589 CCCGGG
SmlI 742 CTTGAG C^T[CT][GA]AG
1320 CTCGAG
StyI 1118 CCATGG C^C[AT][AT]GG
1216 CCAAGG
Tsp45I 133 GTCAC ^GT[GC]AC
154 GTGAC
XmaCI 35 CCCGGG C^CCGGG
589 CCCGGG
XmaI 35 CCCGGG C^CCGGG
589 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AvaI AvaII BanII
BbvI BcnI BmyI BpmI BsaHI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspHI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PpuMI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
TaqI TseI TspRI TthHB8I XhoII