(Circular) (Six-base) MAPSORT of: pFN21AB7416.seq Check: 7286 from: 1 to: 6106 pFN21AB7416 6106 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2175 3916 278 Size: 53 83 186 936 639 1741 2468 Fragments arranged by size: 2468 1741 936 639 186 83 53 AccI GT'mk_AC Cuts at: 1937 3449 1937 Size: 1512 4594 Acc65I G'GTAC_C Cuts at: 3427 3427 Size: 6106 AclI AA'CG_TT Cuts at: 1563 4233 4606 1563 Size: 2670 373 3063 Fragments arranged by size: 3063 2670 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2686 2893 3251 4169 5553 5821 1632 Size: 1054 207 358 918 1384 268 1917 Fragments arranged by size: 1917 1384 1054 918 358 268 207 AfeI AGC'GCT Cuts at: 2712 2712 Size: 6106 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5909 AflIII A'CryG_T Cuts at: 1203 3240 3292 5005 5982 1203 Size: 2037 52 1713 977 1327 Fragments arranged by size: 2037 1713 1327 977 52 AgeI A'CCGG_T Cuts at: 4937 4937 Size: 6106 AhdI GACnn_n'nnGTC Cuts at: 4835 4835 Size: 6106 AleI CACnn'nnGTG Cuts at: 1166 3053 3356 1166 Size: 1887 303 3916 Fragments arranged by size: 3916 1887 303 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6106 AlwNI CAG_nnn'CTG Cuts at: 2131 3000 3079 3136 3266 3849 5421 2131 Size: 869 79 57 130 583 1572 2816 Fragments arranged by size: 2816 1572 869 583 130 79 57 ApaI G_GGCC'C Cuts at: 2602 2950 2602 Size: 348 5758 ApaLI G'TGCA_C Cuts at: 4163 5319 4163 Size: 1156 4950 ApoI r'AATT_y Cuts at: 1493 3415 3641 3793 5902 1493 Size: 1922 226 152 2109 1697 Fragments arranged by size: 2109 1922 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4660 160 Size: 872 3628 1606 Fragments arranged by size: 3628 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6106 AvaI C'yCGr_G Cuts at: 1870 1943 2040 2594 2724 3325 3431 1870 Size: 73 97 554 130 601 106 4545 Fragments arranged by size: 4545 601 554 130 106 97 73 BamHI G'GATC_C Cuts at: 2633 3270 3436 3803 2633 Size: 637 166 367 4936 Fragments arranged by size: 4936 637 367 166 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2231 2805 3427 4882 618 Size: 325 217 627 444 574 622 1455 1842 Fragments arranged by size: 1842 1455 627 622 574 444 325 217 BanII G_rGCy'C Cuts at: 1391 2602 2925 2950 3332 3366 3425 1391 Size: 1211 323 25 382 34 59 4072 Fragments arranged by size: 4072 1211 382 323 59 34 25 BbeI G_GCGC'C Cuts at: 2235 2235 Size: 6106 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5135 BbvCI CC'TCA_GC Cuts at: 2507 2993 2507 Size: 486 5620 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2155 2189 4297 4331 1903 Size: 34 218 34 2108 34 3678 Fragments arranged by size: 3678 2108 218 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3998 5208 3998 Size: 1210 4896 BclI T'GATC_A Cuts at: 1548 1548 Size: 6106 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3257 4717 136 Size: 107 122 71 631 605 1585 1460 1525 Fragments arranged by size: 1585 1525 1460 631 605 122 107 71 BglII A'GATC_T Cuts at: 6101 6101 Size: 6106 BlpI GC'TnA_GC Cuts at: 3523 3523 Size: 6106 Bme1580I G_kGCm'C Cuts at: 1790 2602 2950 4167 5323 1790 Size: 812 348 1217 1156 2573 Fragments arranged by size: 2573 1217 1156 812 348 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6106 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2857 3109 3837 Size: 416 53 83 399 24 1440 252 728 Cuts at: 3837 4795 442 Size: 958 1753 Fragments arranged by size: 1753 1440 958 728 416 399 252 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6106 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3008 3008 Size: 6106 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2633 2948 3158 3299 4766 1239 Size: 1394 315 210 141 1467 2579 Fragments arranged by size: 2579 1467 1394 315 210 141 Bpu10I CC'TnA_GC Cuts at: 2507 2993 2507 Size: 486 5620 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2768 4231 5096 5394 2768 Size: 1463 865 298 3480 Fragments arranged by size: 3480 1463 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4769 882 Size: 617 3270 2219 Fragments arranged by size: 3270 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3309 3897 493 Size: 711 2105 588 2702 Fragments arranged by size: 2702 2105 711 588 BsaBI GATnn'nnATC Cuts at: 6100 6100 Size: 6106 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2172 2232 2306 2841 Size: 53 83 186 936 639 60 74 535 Cuts at: 2841 3177 3913 4295 4930 275 Size: 336 736 382 635 1451 Fragments arranged by size: 1451 936 736 639 635 535 382 336 186 83 74 60 53 BsaWI w'CCGG_w Cuts at: 4539 4937 5211 5358 5769 4539 Size: 398 274 147 411 4876 Fragments arranged by size: 4876 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2681 2711 3384 3414 1178 Size: 30 165 30 1278 30 673 30 3870 Fragments arranged by size: 3870 1278 673 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2225 2228 3029 1186 Size: 1039 3 801 4263 Fragments arranged by size: 4263 1039 801 3 BseYI C'CCAG_C Cuts at: 2713 2813 3180 3266 3332 5309 5805 2713 Size: 100 367 86 66 1977 496 3014 Fragments arranged by size: 3014 1977 496 367 100 86 66 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2198 2207 2264 2691 3170 3324 1163 Size: 573 462 9 57 427 479 154 3945 Fragments arranged by size: 3945 573 479 462 427 154 57 9 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2035 3556 4317 5345 1540 Size: 275 186 34 1521 761 1028 2301 Fragments arranged by size: 2301 1521 1028 761 275 186 34 BsiHKAI G_wGCw'C Cuts at: 2658 2925 4167 4252 5323 2658 Size: 267 1242 85 1071 3441 Fragments arranged by size: 3441 1242 1071 267 85 BsmI GAATG_Cn' Cuts at: 2863 3617 3710 2863 Size: 754 93 5259 Fragments arranged by size: 5259 754 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2209 2297 3283 2209 Size: 88 986 5032 Fragments arranged by size: 5032 986 88 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2602 2658 2925 2950 3332 3366 3425 Size: 399 812 56 267 25 382 34 59 Cuts at: 3425 4167 4252 5323 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 812 742 399 382 267 85 59 56 34 25 BspEI T'CCGG_A Cuts at: 5769 5769 Size: 6106 BspHI T'CATG_A Cuts at: 2658 2853 3201 3995 5725 2658 Size: 195 348 794 1730 3039 Fragments arranged by size: 3039 1730 794 348 195 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3461 844 Size: 2617 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2074 3993 1394 Size: 429 251 1919 3507 Fragments arranged by size: 3507 1919 429 251 BsrDI GCAATG_nn' Cuts at: 65 1248 2547 4601 4775 65 Size: 1183 1299 2054 174 1396 Fragments arranged by size: 2054 1396 1299 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2012 2353 2445 4750 4937 1700 Size: 110 202 341 92 2305 187 2869 Fragments arranged by size: 2869 2305 341 202 187 110 92 BsrGI T'GTAC_A Cuts at: 96 3243 96 Size: 3147 2959 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6106 BssSI C'ACGA_G Cuts at: 2795 4166 5178 2795 Size: 1371 1012 3723 Fragments arranged by size: 3723 1371 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6106 BstBI TT'CG_AA Cuts at: 4910 4910 Size: 6106 BstEII G'GTnAC_C Cuts at: 1184 2137 1184 Size: 953 5153 BstXI CCAn_nnnn'nTGG Cuts at: 2383 3055 3358 2383 Size: 672 303 5131 Fragments arranged by size: 5131 672 303 BstYI r'GATC_y Cuts at: 1972 2633 2826 3270 3436 3803 4188 4205 5657 Size: 661 193 444 166 367 385 17 1452 Cuts at: 5657 5743 5755 6101 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 661 444 385 367 346 193 166 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2348 2348 Size: 6106 BtgI C'CryG_G Cuts at: 513 1065 2004 2865 2937 3123 3317 513 Size: 552 939 861 72 186 194 3302 Fragments arranged by size: 3302 939 861 552 194 186 72 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5367 Fragments arranged by size: 5367 683 56 BtsI GCAGTG_nn' Cuts at: 2186 3622 4416 4436 2186 Size: 1436 794 20 3856 Fragments arranged by size: 3856 1436 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6106 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6071 DraI TTT'AAA Cuts at: 3410 3410 Size: 6106 DraIII CAC_nnn'GTG Cuts at: 2158 2158 Size: 6106 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2543 5113 809 Size: 507 1227 2570 1802 Fragments arranged by size: 2570 1802 1227 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2032 2294 Size: 54 1425 50 275 18 177 25 262 Cuts at: 2294 2385 2770 3402 3553 4442 6071 8 Size: 91 385 632 151 889 1629 43 Fragments arranged by size: 1629 1425 889 632 385 275 262 177 151 91 54 50 43 25 18 EagI C'GGCC_G Cuts at: 1537 1812 2032 3553 1537 Size: 275 220 1521 4090 Fragments arranged by size: 4090 1521 275 220 EarI CTCTTCn'nnn_ Cuts at: 1353 3281 4036 1353 Size: 1928 755 3423 Fragments arranged by size: 3423 1928 755 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4693 5067 5213 387 Size: 1064 138 3104 374 146 1280 Fragments arranged by size: 3104 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2923 2923 Size: 6106 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2633 2686 2893 2948 3158 3251 3299 Size: 393 1001 53 207 55 210 93 48 Cuts at: 3299 4169 4766 5553 5821 1239 Size: 870 597 787 268 1524 Fragments arranged by size: 1524 1001 870 787 597 393 268 210 207 93 55 53 48 EcoNI CCTnn'n_nnAGG Cuts at: 3220 3260 3220 Size: 40 6066 EcoO109I rG'GnC_Cy Cuts at: 2095 2218 2412 2476 2586 2598 2599 2721 2932 Size: 123 194 64 110 12 1 122 211 Cuts at: 2932 3043 3250 2095 Size: 111 207 4951 Fragments arranged by size: 4951 211 207 194 123 122 111 110 64 12 1 EcoRI G'AATT_C Cuts at: 3415 3793 3415 Size: 378 5728 FspI TGC'GCA Cuts at: 4612 4612 Size: 6106 HaeII r_GCGC'y Cuts at: 1678 2131 2235 2714 5253 1678 Size: 453 104 479 2539 2531 Fragments arranged by size: 2539 2531 479 453 104 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2041 2073 4747 4779 4821 Size: 32 135 32 469 32 2674 32 42 Cuts at: 4821 4853 1373 Size: 32 2626 Fragments arranged by size: 2674 2626 469 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3106 3450 3696 669 Size: 988 281 1168 344 246 3079 Fragments arranged by size: 3079 1168 988 344 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6106 HpaI GTT'AAC Cuts at: 3696 3696 Size: 6106 KasI G'GCGC_C Cuts at: 2231 2231 Size: 6106 KpnI G_GTAC'C Cuts at: 3431 3431 Size: 6106 MfeI C'AATT_G Cuts at: 3705 3705 Size: 6106 MluI A'CGCG_T Cuts at: 3292 5982 3292 Size: 2690 3416 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5220 5404 1305 Size: 3915 184 2007 Fragments arranged by size: 3915 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2387 10 Size: 54 1425 343 555 3729 Fragments arranged by size: 3729 1425 555 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3053 3162 3194 3200 3356 4064 4423 Size: 648 1887 109 32 6 156 708 359 Cuts at: 4423 4582 518 Size: 159 2042 Fragments arranged by size: 2042 1887 708 648 359 159 156 109 32 6 MspA1I CmG'CkG Cuts at: 1606 2266 2506 3184 3277 3371 3522 4199 5347 Size: 660 240 678 93 94 151 677 1148 Cuts at: 5347 5592 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 678 677 660 245 240 151 94 93 NaeI GCC'GGC Cuts at: 2014 2355 2014 Size: 341 5765 NarI GG'CG_CC Cuts at: 2232 2232 Size: 6106 NcoI C'CATG_G Cuts at: 513 1065 2004 3123 513 Size: 552 939 1119 3496 Fragments arranged by size: 3496 1119 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6106 NgoMIV G'CCGG_C Cuts at: 2012 2353 2012 Size: 341 5765 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6106 NotI GC'GGCC_GC Cuts at: 3553 3553 Size: 6106 NspI r_CATG'y Cuts at: 1132 1229 2092 3012 3199 3244 3464 5009 1132 Size: 97 863 920 187 45 220 1545 2229 Fragments arranged by size: 2229 1545 920 863 220 187 97 45 PasI CC'CwG_GG Cuts at: 1348 3383 1348 Size: 2035 4071 PciI A'CATG_T Cuts at: 3240 5005 3240 Size: 1765 4341 PflMI CCAn_nnn'nTGG Cuts at: 1108 2956 1108 Size: 1848 4258 PfoI T'CCnGG_A Cuts at: 2916 2925 3168 2916 Size: 9 243 5854 Fragments arranged by size: 5854 243 9 PmeI GTTT'AAAC Cuts at: 3410 3410 Size: 6106 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4175 4207 5688 5720 4175 Size: 32 1481 32 4561 Fragments arranged by size: 4561 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2095 2412 2476 2721 2932 3043 2095 Size: 317 64 245 211 111 5158 Fragments arranged by size: 5158 317 245 211 111 64 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6106 PsiI TTA'TAA Cuts at: 3676 3676 Size: 6106 PspOMI G'GGCC_C Cuts at: 2598 2946 2598 Size: 348 5758 PspXI vC'TCGA_Gb Cuts at: 3325 3325 Size: 6106 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3059 3091 3059 Size: 32 6074 PstI C_TGCA'G Cuts at: 830 2362 3458 830 Size: 1532 1096 3478 Fragments arranged by size: 3478 1532 1096 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6106 PvuII CAG'CTG Cuts at: 2506 3184 2506 Size: 678 5428 SacI G_AGCT'C Cuts at: 2925 2925 Size: 6106 SalI G'TCGA_C Cuts at: 1936 3448 1936 Size: 1512 4594 SanDI GG'GwC_CC Cuts at: 2412 2412 Size: 6106 SbfI CC_TGCA'GG Cuts at: 3458 3458 Size: 6106 ScaI AGT'ACT Cuts at: 2699 4354 6066 2699 Size: 1655 1712 2739 Fragments arranged by size: 2739 1712 1655 SexAI A'CCwGG_T Cuts at: 2478 2478 Size: 6106 SfcI C'TryA_G Cuts at: 826 1045 2311 2358 3454 4589 5270 5461 826 Size: 219 1266 47 1096 1135 681 191 1471 Fragments arranged by size: 1471 1266 1135 1096 681 219 191 47 SfoI GGC'GCC Cuts at: 2233 2233 Size: 6106 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6106 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6106 SmaI CCC'GGG Cuts at: 1872 2042 2596 3433 1872 Size: 170 554 837 4545 Fragments arranged by size: 4545 837 554 170 SmlI C'TyrA_G Cuts at: 820 1017 1943 2747 3325 4210 5111 5373 820 Size: 197 926 804 578 885 901 262 1553 Fragments arranged by size: 1553 926 901 885 804 578 262 197 SnaBI TAC'GTA Cuts at: 493 3897 493 Size: 3404 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6106 SphI G_CATG'C Cuts at: 1132 2092 3464 1132 Size: 960 1372 3774 Fragments arranged by size: 3774 1372 960 SspI AAT'ATT Cuts at: 5 52 4030 5 Size: 47 3978 2081 Fragments arranged by size: 3978 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6106 StyI C'CwwG_G Cuts at: 513 1065 2004 3123 3221 3545 513 Size: 552 939 1119 98 324 3074 Fragments arranged by size: 3074 1119 939 552 324 98 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4133 4150 4303 4488 4933 325 Size: 1255 2553 17 153 185 445 1498 Fragments arranged by size: 2553 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2697 3243 4352 Size: 274 80 33 51 1443 720 546 1109 Cuts at: 4352 6064 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1109 720 546 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2131 2163 3384 3416 6066 6098 2131 Size: 32 1221 32 2650 32 2139 Fragments arranged by size: 2650 2139 1221 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6106 XbaI T'CTAG_A Cuts at: 3442 3442 Size: 6106 XhoI C'TCGA_G Cuts at: 1943 3325 1943 Size: 1382 4724 XmaI C'CCGG_G Cuts at: 1870 2040 2594 3431 1870 Size: 170 554 837 4545 Fragments arranged by size: 4545 837 554 170 XmnI GAAnn'nnTTC Cuts at: 4235 4235 Size: 6106 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2173 3914 276 Size: 53 83 186 936 639 1741 2468 Fragments arranged by size: 2468 1741 936 639 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI RsrII SacII SapI SfiI SrfI SwaI XcmI