(Circular) (Six-base) MAPSORT of: pFN21AB7407.seq Check: 7737 from: 1 to: 5344 pFN21AB7407 5344 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3154 278 Size: 53 83 186 936 1618 2468 Fragments arranged by size: 2468 1618 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2121 2687 1937 Size: 184 566 4594 Fragments arranged by size: 4594 566 184 Acc65I G'GTAC_C Cuts at: 2665 2665 Size: 5344 AclI AA'CG_TT Cuts at: 1563 3471 3844 1563 Size: 1908 373 3063 Fragments arranged by size: 3063 1908 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2522 3407 4791 5059 1632 Size: 890 885 1384 268 1917 Fragments arranged by size: 1917 1384 890 885 268 AfeI AGC'GCT Cuts at: 2336 2336 Size: 5344 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5147 AflIII A'CryG_T Cuts at: 1203 4243 5220 1203 Size: 3040 977 1327 Fragments arranged by size: 3040 1327 977 AgeI A'CCGG_T Cuts at: 4175 4175 Size: 5344 AhdI GACnn_n'nnGTC Cuts at: 4073 4073 Size: 5344 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5344 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5344 AlwNI CAG_nnn'CTG Cuts at: 3087 4659 3087 Size: 1572 3772 ApaLI G'TGCA_C Cuts at: 3401 4557 3401 Size: 1156 4188 ApoI r'AATT_y Cuts at: 1493 2653 2879 3031 5140 1493 Size: 1160 226 152 2109 1697 Fragments arranged by size: 2109 1697 1160 226 152 AseI AT'TA_AT Cuts at: 160 1032 3898 160 Size: 872 2866 1606 Fragments arranged by size: 2866 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5344 AvaI C'yCGr_G Cuts at: 1870 1943 2363 2584 2669 1870 Size: 73 420 221 85 4545 Fragments arranged by size: 4545 420 221 85 73 BamHI G'GATC_C Cuts at: 2674 3041 2674 Size: 367 4977 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 2236 2359 2665 4120 Size: 325 217 627 221 228 123 306 1455 Cuts at: 4120 618 Size: 1842 Fragments arranged by size: 1842 1455 627 325 306 228 221 217 123 BanII G_rGCy'C Cuts at: 1391 2553 2663 1391 Size: 1162 110 4072 Fragments arranged by size: 4072 1162 110 BbeI G_GCGC'C Cuts at: 2012 2240 2363 2012 Size: 228 123 4993 Fragments arranged by size: 4993 228 123 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4373 BbvCI CC'TCA_GC Cuts at: 2606 2606 Size: 5344 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3535 3569 1903 Size: 34 1598 34 3678 Fragments arranged by size: 3678 1598 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3236 4446 3236 Size: 1210 4134 BclI T'GATC_A Cuts at: 1548 1548 Size: 5344 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2325 2368 2589 Size: 107 122 71 631 605 653 43 221 Cuts at: 2589 3955 136 Size: 1366 1525 Fragments arranged by size: 1525 1366 653 631 605 221 122 107 71 43 BglII A'GATC_T Cuts at: 5339 5339 Size: 5344 BlpI GC'TnA_GC Cuts at: 2761 2761 Size: 5344 Bme1580I G_kGCm'C Cuts at: 1790 3405 4561 1790 Size: 1615 1156 2573 Fragments arranged by size: 2573 1615 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5344 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3075 4033 442 Size: 416 53 83 399 24 1658 958 1753 Fragments arranged by size: 1753 1658 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5344 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4004 1239 Size: 2765 2579 Bpu10I CC'TnA_GC Cuts at: 2547 2606 2547 Size: 59 5285 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2360 3469 4334 4632 2360 Size: 1109 865 298 3072 Fragments arranged by size: 3072 1109 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2522 4007 882 Size: 617 1023 1485 2219 Fragments arranged by size: 2219 1485 1023 617 BsaAI yAC'GTr Cuts at: 493 1204 2272 2512 3135 493 Size: 711 1068 240 623 2702 Fragments arranged by size: 2702 1068 711 623 240 BsaBI GATnn'nnATC Cuts at: 5338 5338 Size: 5344 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2088 2237 2360 Size: 53 83 186 936 476 79 149 123 Cuts at: 2360 3151 3533 4168 275 Size: 791 382 635 1451 Fragments arranged by size: 1451 936 791 635 476 382 186 149 123 83 79 53 BsaWI w'CCGG_w Cuts at: 3777 4175 4449 4596 5007 3777 Size: 398 274 147 411 4114 Fragments arranged by size: 4114 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5119 Fragments arranged by size: 5119 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5344 BseYI C'CCAG_C Cuts at: 2337 4547 5043 2337 Size: 2210 496 2638 Fragments arranged by size: 2638 2210 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4771 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2253 2519 2794 3555 4583 1540 Size: 275 186 252 266 275 761 1028 2301 Fragments arranged by size: 2301 1028 761 275 275 266 252 186 BsiHKAI G_wGCw'C Cuts at: 2268 2625 3405 3490 4561 2268 Size: 357 780 85 1071 3051 Fragments arranged by size: 3051 1071 780 357 85 BsmI GAATG_Cn' Cuts at: 2855 2948 2855 Size: 93 5251 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2268 2553 2625 2663 3405 3490 4561 Size: 399 478 285 72 38 742 85 1071 Cuts at: 4561 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 478 399 285 85 72 38 BspEI T'CCGG_A Cuts at: 5007 5007 Size: 5344 BspHI T'CATG_A Cuts at: 3233 4963 3233 Size: 1730 3614 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2699 844 Size: 1855 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2374 3231 1394 Size: 429 551 857 3507 Fragments arranged by size: 3507 857 551 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3839 4013 65 Size: 1183 2591 174 1396 Fragments arranged by size: 2591 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2248 2349 2484 3988 4175 1700 Size: 110 438 101 135 1504 187 2869 Fragments arranged by size: 2869 1504 438 187 135 110 101 BsrGI T'GTAC_A Cuts at: 96 2277 96 Size: 2181 3163 BssHII G'CGCG_C Cuts at: 1925 2075 2077 1925 Size: 150 2 5192 Fragments arranged by size: 5192 150 2 BssSI C'ACGA_G Cuts at: 2441 2572 3404 4416 2441 Size: 131 832 1012 3369 Fragments arranged by size: 3369 1012 832 131 BstAPI GCAn_nnn'nTGC Cuts at: 794 2272 794 Size: 1478 3866 BstBI TT'CG_AA Cuts at: 4148 4148 Size: 5344 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5344 BstXI CCAn_nnnn'nTGG Cuts at: 2479 2479 Size: 5344 BstYI r'GATC_y Cuts at: 1972 2674 3041 3426 3443 4895 4981 4993 5339 Size: 702 367 385 17 1452 86 12 346 Cuts at: 5339 1972 Size: 1977 Fragments arranged by size: 1977 1452 702 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2355 513 Size: 552 939 351 3502 Fragments arranged by size: 3502 939 552 351 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4605 Fragments arranged by size: 4605 683 56 BtsI GCAGTG_nn' Cuts at: 2860 3654 3674 2860 Size: 794 20 4530 Fragments arranged by size: 4530 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5344 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5309 DraI TTT'AAA Cuts at: 2648 2648 Size: 5344 DraIII CAC_nnn'GTG Cuts at: 2272 2272 Size: 5344 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4351 809 Size: 507 3035 1802 Fragments arranged by size: 3035 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2250 2437 2516 Size: 54 1425 50 275 18 420 187 79 Cuts at: 2516 2791 3680 5309 8 Size: 275 889 1629 43 Fragments arranged by size: 1629 1425 889 420 275 275 187 79 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2250 2516 2791 1537 Size: 275 438 266 275 4090 Fragments arranged by size: 4090 438 275 275 266 EarI CTCTTCn'nnn_ Cuts at: 1353 2405 2606 3274 1353 Size: 1052 201 668 3423 Fragments arranged by size: 3423 1052 668 201 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3931 4305 4451 387 Size: 1064 138 2342 374 146 1280 Fragments arranged by size: 2342 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2522 3407 4004 4791 5059 1239 Size: 393 890 885 597 787 268 1524 Fragments arranged by size: 1524 890 885 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2537 2537 Size: 5344 EcoRI G'AATT_C Cuts at: 2653 3031 2653 Size: 378 4966 FspI TGC'GCA Cuts at: 3850 3850 Size: 5344 HaeII r_GCGC'y Cuts at: 1678 2012 2023 2240 2338 2363 4491 1678 Size: 334 11 217 98 25 2128 2531 Fragments arranged by size: 2531 2128 334 217 98 25 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3985 4017 4059 4091 1373 Size: 32 135 32 2413 32 42 32 2626 Fragments arranged by size: 2626 2413 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2287 2688 2934 669 Size: 988 281 349 401 246 3079 Fragments arranged by size: 3079 988 401 349 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5344 HpaI GTT'AAC Cuts at: 2934 2934 Size: 5344 KasI G'GCGC_C Cuts at: 2008 2236 2359 2008 Size: 228 123 4993 Fragments arranged by size: 4993 228 123 KpnI G_GTAC'C Cuts at: 2669 2669 Size: 5344 MfeI C'AATT_G Cuts at: 2943 2943 Size: 5344 MluI A'CGCG_T Cuts at: 5220 5220 Size: 5344 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4458 4642 1305 Size: 3153 184 2007 Fragments arranged by size: 3153 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3522 Fragments arranged by size: 3522 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2618 3302 3661 3820 518 Size: 648 1452 684 359 159 2042 Fragments arranged by size: 2042 1452 684 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2026 2035 2056 2242 2357 2760 3437 4585 Size: 420 9 21 186 115 403 677 1148 Cuts at: 4585 4830 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 420 403 245 186 115 21 9 NaeI GCC'GGC Cuts at: 2250 2250 Size: 5344 NarI GG'CG_CC Cuts at: 2009 2237 2360 2009 Size: 228 123 4993 Fragments arranged by size: 4993 228 123 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3853 Fragments arranged by size: 3853 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5344 NgoMIV G'CCGG_C Cuts at: 2248 2248 Size: 5344 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5344 NotI GC'GGCC_GC Cuts at: 2791 2791 Size: 5344 NspI r_CATG'y Cuts at: 1132 1229 2702 4247 1132 Size: 97 1473 1545 2229 Fragments arranged by size: 2229 1545 1473 97 PasI CC'CwG_GG Cuts at: 1348 2416 1348 Size: 1068 4276 PciI A'CATG_T Cuts at: 4243 4243 Size: 5344 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5344 PmeI GTTT'AAAC Cuts at: 2648 2648 Size: 5344 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3413 3445 4926 4958 3413 Size: 32 1481 32 3799 Fragments arranged by size: 3799 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5344 PsiI TTA'TAA Cuts at: 2914 2914 Size: 5344 PstI C_TGCA'G Cuts at: 830 2696 830 Size: 1866 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5344 RsrII CG'GwC_CG Cuts at: 2107 2107 Size: 5344 SacII CC_GC'GG Cuts at: 2358 2358 Size: 5344 SalI G'TCGA_C Cuts at: 1936 2686 1936 Size: 750 4594 SapI GCTCTTCn'nnn_ Cuts at: 2405 2405 Size: 5344 SbfI CC_TGCA'GG Cuts at: 2696 2696 Size: 5344 ScaI AGT'ACT Cuts at: 3592 5304 3592 Size: 1712 3632 SfcI C'TryA_G Cuts at: 826 1045 2692 3827 4508 4699 826 Size: 219 1647 1135 681 191 1471 Fragments arranged by size: 1647 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2010 2238 2361 2010 Size: 228 123 4993 Fragments arranged by size: 4993 228 123 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5344 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5344 SmaI CCC'GGG Cuts at: 1872 2365 2586 2671 1872 Size: 493 221 85 4545 Fragments arranged by size: 4545 493 221 85 SmlI C'TyrA_G Cuts at: 820 1017 1943 2375 3448 4349 4611 820 Size: 197 926 432 1073 901 262 1553 Fragments arranged by size: 1553 1073 926 901 432 262 197 SnaBI TAC'GTA Cuts at: 493 3135 493 Size: 2642 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5344 SphI G_CATG'C Cuts at: 1132 2702 1132 Size: 1570 3774 SrfI GCCC'GGGC Cuts at: 2586 2586 Size: 5344 SspI AAT'ATT Cuts at: 5 52 3268 5 Size: 47 3216 2081 Fragments arranged by size: 3216 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5344 StyI C'CwwG_G Cuts at: 513 1065 2004 2783 513 Size: 552 939 779 3074 Fragments arranged by size: 3074 939 779 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3371 3388 3541 3726 4171 325 Size: 1255 1791 17 153 185 445 1498 Fragments arranged by size: 1791 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2277 3590 5302 Size: 274 80 33 51 1443 300 1313 1712 Cuts at: 5302 96 Size: 138 Fragments arranged by size: 1712 1443 1313 300 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5304 5336 5304 Size: 32 5312 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5344 XbaI T'CTAG_A Cuts at: 2680 2680 Size: 5344 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5344 XmaI C'CCGG_G Cuts at: 1870 2363 2584 2669 1870 Size: 493 221 85 4545 Fragments arranged by size: 4545 493 221 85 XmnI GAAnn'nnTTC Cuts at: 3473 3473 Size: 5344 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3152 276 Size: 53 83 186 936 1618 2468 Fragments arranged by size: 2468 1618 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI RsrII SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII SacI SanDI SexAI SfiI SwaI XcmI