Restriction map for
pFN21AB7405
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# NO CUT ENZYMES ( 111 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AlwI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BalI
BamHI BbrPI BbvCI BclI BfrI
BglI BglII BlnI BsaAI BsaBI
BsaWI BseAI BseRI BsiWI BsmI
BspEI BspHI BspLU11I BsrBI BsrDI
BssSI Bst1107I BstBI BstEII BstPI
BstYI BstZ17I CpoI DraI DrdI
Eam1105I Eco47III EcoNI EcoO65I EcoRI
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI MamI MfeI MflI
MluI MluNI MroI MscI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Van91I XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcsI 1094 AAATTC [GA]^AATT[CT]
Alw44I 1003 GTGCAC G^TGCAC
AlwNI 466 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1300 GGGCCC GGGCC^C
ApaLI 1003 GTGCAC G^TGCAC
ApoI 1094 AAATTC [GA]^AATT[CT]
BanI 185 GGCGCC G^G[CT][GA]CC
BanII 1300 GGGCCC G[GA]GC[CT]^C
BbeI 185 GGCGCC GGCGC^C
BcgI 95 GCAACTCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1056 GTATCC GTATCC(6/5)
BfaI 1309 CTAG C^TAG
BpmI 1171 CTGGAG CTGGAG(16/14)
BsgI 1045 CTGCAC GTGCAG(16/14)
BsmBI 1343 GAGACG CGTCTC(1/5)
Bsp1407I 993 TGTACA T^GTACA
BspDI 328 ATCGAT AT^CGAT
BspMI 637 GCAGGT ACCTGC(4/8)
BsrGI 993 TGTACA T^GTACA
BstAPI 17 GCAAGACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 1137 CATCC GGATG(2/0)
BstXI 284 CCATCTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 328 ATCGAT AT^CGAT
DpnII 144 GATC ^GATC
DraIII 1126 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 566 CGGCCG C^GGCCG
EclXI 566 CGGCCG C^GGCCG
Eco52I 566 CGGCCG C^GGCCG
EcoRV 1073 GATATC GAT^ATC
EcoT14I 1217 CCAAGG C^C[AT][AT]GG
FokI 1137 CATCC GGATG(9/13)
KasI 185 GGCGCC G^GCGCC
KspI 526 CCGCGG CCGC^GG
MaeI 1309 CTAG C^TAG
MboI 144 GATC ^GATC
MseI 1409 TTAA T^TAA
NarI 185 GGCGCC GG^CGCC
NdeII 144 GATC ^GATC
PstI 454 CTGCAG CTGCA^G
SacII 526 CCGCGG CCGC^GG
Sau3AI 144 GATC ^GATC
SfoI 185 GGCGCC GGC^GCC
SspBI 993 TGTACA T^GTACA
StyI 1217 CCAAGG C^C[AT][AT]GG
Tru9I 1409 TTAA T^TAA
XbaI 1308 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcyI 185 GGCGCC G[GA]^CG[CT]C
358 GGCGTC
AfaI 721 GTAC GT^AC
994 GTAC
AocI 508 CCTCAGG CC^T[ACGT]AGG
1060 CCTAAGG
AspI 440 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1400 GACAAAGTC
BbsI 670 GAAGAC GAAGAC(2/6)
259 GTCTTC
BpuAI 670 GAAGAC GAAGAC(2/6)
259 GTCTTC
BsaHI 185 GGCGCC G[GA]^CG[CT]C
358 GGCGTC
BsaI 901 GAGACC GGTCTC(1/5)
78 GGTCTC
BsiEI 566 CGGCCG CG[GA][CT]^CG
617 CGACCG
BsrFI 26 GCCGGC [GA]^CCGG[CT]
169 GCCGGC
BsrI 821 ACTGG ACTGG(1/-1)
1140 CCAGT
BssHII 242 GCGCGC G^CGCGC
531 GCGCGC
Bsu36I 508 CCTCAGG CC^T[ACGT]AGG
1060 CCTAAGG
Cfr10I 26 GCCGGC [GA]^CCGG[CT]
169 GCCGGC
EaeI 566 CGGCCG [CT]^GGCC[GA]
773 CGGCCA
Eco81I 508 CCTCAGG CC^T[ACGT]AGG
1060 CCTAAGG
Hin1I 185 GGCGCC G[GA]^CG[CT]C
358 GGCGTC
NaeI 26 GCCGGC GCC^GGC
169 GCCGGC
NgoMIV 26 GCCGGC G^CCGGC
169 GCCGGC
NspI 128 ACATGC [GA]CATG^[CT]
729 GCATGT
PflFI 440 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1400 GACAAAGTC
PvuII 469 CAGCTG CAG^CTG
1234 CAGCTG
RsaI 721 GTAC GT^AC
994 GTAC
SapI 949 GAAGAGC GCTCTTC(1/4)
33 GCTCTTC
SfcI 454 CTGCAG C^T[GA][CT]AG
919 CTACAG
SmaI 194 CCCGGG CCC^GGG
1249 CCCGGG
TaqI 329 TCGA T^CGA
1098 TCGA
TfiI 643 GATTC G^A[AT]TC
1121 GAATC
Tsp45I 1132 GTGAC ^GT[GC]AC
1391 GTGAC
Tsp509I 984 AATT ^AATT
1095 AATT
Tth111I 440 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1400 GACAAAGTC
TthHB8I 329 TCGA T^CGA
1098 TCGA
XmaCI 194 CCCGGG C^CCGGG
1249 CCCGGG
XmaI 194 CCCGGG C^CCGGG
1249 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AluI AspHI AvaI
AvaII BbvI BcnI BlpI BmyI
Bpu1102I BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BssKI
BstNI BstUI Cac8I CelII CfoI
Cfr13I DdeI DraII DsaI EarI
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
PleI PpuMI Sau96I ScrFI SfaNI
TseI TspRI