(Circular) (Six-base) MAPSORT of: pFN21AB7405.seq Check: 5995 from: 1 to: 6160 pFN21AB7405 6160 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2633 2633 Size: 6160 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3970 278 Size: 53 83 186 936 2434 2468 Fragments arranged by size: 2468 2434 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3503 1937 Size: 1566 4594 Acc65I G'GTAC_C Cuts at: 3481 3481 Size: 6160 AclI AA'CG_TT Cuts at: 1563 4287 4660 1563 Size: 2724 373 3063 Fragments arranged by size: 3063 2724 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2191 2724 2825 4223 5607 5875 1632 Size: 559 533 101 1398 1384 268 1917 Fragments arranged by size: 1917 1398 1384 559 533 268 101 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5963 AflIII A'CryG_T Cuts at: 1203 5059 6036 1203 Size: 3856 977 1327 Fragments arranged by size: 3856 1327 977 AgeI A'CCGG_T Cuts at: 4991 4991 Size: 6160 AhdI GACnn_n'nnGTC Cuts at: 4889 4889 Size: 6160 AleI CACnn'nnGTG Cuts at: 1166 3300 1166 Size: 2134 4026 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6160 AlwNI CAG_nnn'CTG Cuts at: 2476 3903 5475 2476 Size: 1427 1572 3161 Fragments arranged by size: 3161 1572 1427 ApaI G_GGCC'C Cuts at: 3309 3309 Size: 6160 ApaLI G'TGCA_C Cuts at: 3008 4217 5373 3008 Size: 1209 1156 3795 Fragments arranged by size: 3795 1209 1156 ApoI r'AATT_y Cuts at: 1493 3099 3469 3695 3847 5956 1493 Size: 1606 370 226 152 2109 1697 Fragments arranged by size: 2109 1697 1606 370 226 152 AseI AT'TA_AT Cuts at: 160 1032 4714 160 Size: 872 3682 1606 Fragments arranged by size: 3682 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6160 AvaI C'yCGr_G Cuts at: 1870 1943 2199 2324 2342 3254 3485 1870 Size: 73 256 125 18 912 231 4545 Fragments arranged by size: 4545 912 256 231 125 73 18 BamHI G'GATC_C Cuts at: 3490 3857 3490 Size: 367 5793 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2190 3481 4936 618 Size: 325 217 627 403 1291 1455 1842 Fragments arranged by size: 1842 1455 1291 627 403 325 217 BanII G_rGCy'C Cuts at: 1391 3309 3479 1391 Size: 1918 170 4072 Fragments arranged by size: 4072 1918 170 BbeI G_GCGC'C Cuts at: 2194 2194 Size: 6160 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2257 2682 928 Size: 971 358 425 4406 Fragments arranged by size: 4406 971 425 358 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2089 2123 4351 4385 1903 Size: 34 152 34 2228 34 3678 Fragments arranged by size: 3678 2228 152 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3072 4052 5262 3072 Size: 980 1210 3970 Fragments arranged by size: 3970 1210 980 BclI T'GATC_A Cuts at: 1548 1548 Size: 6160 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4771 136 Size: 107 122 71 631 605 3099 1525 Fragments arranged by size: 3099 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6155 6155 Size: 6160 BlpI GC'TnA_GC Cuts at: 2252 3234 3291 3577 2252 Size: 982 57 286 4835 Fragments arranged by size: 4835 982 286 57 Bme1580I G_kGCm'C Cuts at: 1790 3012 3262 3309 4221 5377 1790 Size: 1222 250 47 912 1156 2573 Fragments arranged by size: 2573 1222 1156 912 250 47 BmgBI CAC'GTC Cuts at: 1318 2028 3277 1318 Size: 710 1249 4201 Fragments arranged by size: 4201 1249 710 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3891 4849 442 Size: 416 53 83 399 24 2474 958 1753 Fragments arranged by size: 2474 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6160 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2101 2101 Size: 6160 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3197 4820 1239 Size: 1958 1623 2579 Fragments arranged by size: 2579 1958 1623 Bpu10I CC'TnA_GC Cuts at: 2168 2168 Size: 6160 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4285 5150 5448 4285 Size: 865 298 4997 Fragments arranged by size: 4997 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2089 2900 4823 882 Size: 617 590 811 1923 2219 Fragments arranged by size: 2219 1923 811 617 590 BsaAI yAC'GTr Cuts at: 493 1204 3951 493 Size: 711 2747 2702 Fragments arranged by size: 2747 2702 711 BsaBI GATnn'nnATC Cuts at: 6154 6154 Size: 6160 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2191 2364 3967 4349 Size: 53 83 186 936 658 173 1603 382 Cuts at: 4349 4984 275 Size: 635 1451 Fragments arranged by size: 1603 1451 936 658 635 382 186 173 83 53 BsaWI w'CCGG_w Cuts at: 4593 4991 5265 5412 5823 4593 Size: 398 274 147 411 4930 Fragments arranged by size: 4930 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2123 2153 1178 Size: 30 165 30 720 30 5185 Fragments arranged by size: 5185 720 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6160 BseYI C'CCAG_C Cuts at: 2846 5363 5859 2846 Size: 2517 496 3147 Fragments arranged by size: 3147 2517 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3035 1163 Size: 573 1299 4288 Fragments arranged by size: 4288 1299 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2574 2625 3610 4371 5399 1540 Size: 275 186 573 51 985 761 1028 2301 Fragments arranged by size: 2301 1028 985 761 573 275 186 51 BsiHKAI G_wGCw'C Cuts at: 2055 2355 3012 3297 4221 4306 5377 2055 Size: 300 657 285 924 85 1071 2838 Fragments arranged by size: 2838 1071 924 657 300 285 85 BsmI GAATG_Cn' Cuts at: 3671 3764 3671 Size: 93 6067 BsmBI CGTCTCn'nnnn_ Cuts at: 3342 3342 Size: 6160 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 2355 3012 3262 3297 3309 3479 Size: 399 265 300 657 250 35 12 170 Cuts at: 3479 4221 4306 5377 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 657 399 300 265 250 170 85 35 12 BspEI T'CCGG_A Cuts at: 5823 5823 Size: 6160 BspHI T'CATG_A Cuts at: 4049 5779 4049 Size: 1730 4430 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2633 3515 844 Size: 1789 882 3489 Fragments arranged by size: 3489 1789 882 BsrBI CCG'CTC Cuts at: 1394 1823 4047 1394 Size: 429 2224 3507 Fragments arranged by size: 3507 2224 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4655 4829 65 Size: 1183 3407 174 1396 Fragments arranged by size: 3407 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2031 2174 4804 4991 1700 Size: 110 221 143 2630 187 2869 Fragments arranged by size: 2869 2630 221 187 143 110 BsrGI T'GTAC_A Cuts at: 96 2998 96 Size: 2902 3258 BssHII G'CGCG_C Cuts at: 1925 2247 2536 1925 Size: 322 289 5549 Fragments arranged by size: 5549 322 289 BssSI C'ACGA_G Cuts at: 4220 5232 4220 Size: 1012 5148 BstAPI GCAn_nnn'nTGC Cuts at: 794 2028 794 Size: 1234 4926 BstBI TT'CG_AA Cuts at: 4964 4964 Size: 6160 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6160 BstXI CCAn_nnnn'nTGG Cuts at: 2296 2296 Size: 6160 BstYI r'GATC_y Cuts at: 1972 3490 3857 4242 4259 5711 5797 5809 6155 Size: 1518 367 385 17 1452 86 12 346 Cuts at: 6155 1972 Size: 1977 Fragments arranged by size: 1977 1518 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2514 3066 2514 Size: 552 5608 BtgI C'CryG_G Cuts at: 513 1065 2004 2531 2760 3400 513 Size: 552 939 527 229 640 3273 Fragments arranged by size: 3273 939 640 552 527 229 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2092 2167 487 Size: 683 56 866 75 4480 Fragments arranged by size: 4480 866 683 75 56 BtsI GCAGTG_nn' Cuts at: 2837 3001 3260 3676 4470 4490 2837 Size: 164 259 416 794 20 4507 Fragments arranged by size: 4507 794 416 259 164 20 ClaI AT'CG_AT Cuts at: 1554 2334 1554 Size: 780 5380 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3342 3377 529 Size: 35 2778 35 3312 Fragments arranged by size: 3312 2778 35 35 DraI TTT'AAA Cuts at: 3464 3464 Size: 6160 DraIII CAC_nnn'GTG Cuts at: 3136 3136 Size: 6160 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5167 809 Size: 507 3851 1802 Fragments arranged by size: 3851 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2571 2778 3607 Size: 54 1425 50 275 18 741 207 829 Cuts at: 3607 4496 6125 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 829 741 275 207 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2571 3607 1537 Size: 275 759 1036 4090 Fragments arranged by size: 4090 1036 759 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2045 2801 2949 3340 4090 1353 Size: 692 756 148 391 750 3423 Fragments arranged by size: 3423 756 750 692 391 148 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2090 2495 2558 4747 5121 5267 Size: 1064 138 501 405 63 2189 374 146 Cuts at: 5267 387 Size: 1280 Fragments arranged by size: 2189 1280 1064 501 405 374 146 138 63 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2191 2724 2825 3197 4223 4820 5607 Size: 393 559 533 101 372 1026 597 787 Cuts at: 5607 5875 1239 Size: 268 1524 Fragments arranged by size: 1524 1026 787 597 559 533 393 372 268 101 EcoO109I rG'GnC_Cy Cuts at: 2510 2527 2589 2815 3306 2510 Size: 17 62 226 491 5364 Fragments arranged by size: 5364 491 226 62 17 EcoRI G'AATT_C Cuts at: 3469 3847 3469 Size: 378 5782 EcoRV GAT'ATC Cuts at: 3080 3080 Size: 6160 FspI TGC'GCA Cuts at: 4666 4666 Size: 6160 HaeII r_GCGC'y Cuts at: 1678 2175 2194 2214 2502 5307 1678 Size: 497 19 20 288 2805 2531 Fragments arranged by size: 2805 2531 497 288 20 19 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2135 2167 2684 2716 4801 Size: 32 135 32 563 32 517 32 2085 Cuts at: 4801 4833 4875 4907 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2085 563 517 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2383 2947 3445 3504 3750 669 Size: 988 281 445 564 498 59 246 3079 Fragments arranged by size: 3079 988 564 498 445 281 246 59 HindIII A'AGCT_T Cuts at: 748 748 Size: 6160 HpaI GTT'AAC Cuts at: 3750 3750 Size: 6160 KasI G'GCGC_C Cuts at: 2190 2190 Size: 6160 KpnI G_GTAC'C Cuts at: 3485 3485 Size: 6160 MfeI C'AATT_G Cuts at: 3759 3759 Size: 6160 MluI A'CGCG_T Cuts at: 6036 6036 Size: 6160 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2145 2166 5274 5458 1305 Size: 840 21 3108 184 2007 Fragments arranged by size: 3108 2007 840 184 21 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4338 Fragments arranged by size: 4338 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2048 2294 3279 3300 4118 4477 4636 Size: 648 882 246 985 21 818 359 159 Cuts at: 4636 518 Size: 2042 Fragments arranged by size: 2042 985 882 818 648 359 246 159 21 MspA1I CmG'CkG Cuts at: 1606 2467 2476 2533 2912 3241 3576 4253 5401 Size: 861 9 57 379 329 335 677 1148 Cuts at: 5401 5646 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 861 677 379 335 329 245 57 9 NaeI GCC'GGC Cuts at: 2033 2176 2033 Size: 143 6017 NarI GG'CG_CC Cuts at: 2191 2191 Size: 6160 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4669 Fragments arranged by size: 4669 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6160 NgoMIV G'CCGG_C Cuts at: 2031 2174 2031 Size: 143 6017 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6160 NotI GC'GGCC_GC Cuts at: 3607 3607 Size: 6160 NspI r_CATG'y Cuts at: 1132 1229 2137 2738 3518 5063 1132 Size: 97 908 601 780 1545 2229 Fragments arranged by size: 2229 1545 908 780 601 97 PasI CC'CwG_GG Cuts at: 1348 2752 1348 Size: 1404 4756 PciI A'CATG_T Cuts at: 5059 5059 Size: 6160 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6160 PfoI T'CCnGG_A Cuts at: 2406 2406 Size: 6160 PmeI GTTT'AAAC Cuts at: 3464 3464 Size: 6160 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4229 4261 5742 5774 4229 Size: 32 1481 32 4615 Fragments arranged by size: 4615 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2527 2589 2815 2527 Size: 62 226 5872 Fragments arranged by size: 5872 226 62 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6160 PsiI TTA'TAA Cuts at: 3730 3730 Size: 6160 PspOMI G'GGCC_C Cuts at: 3305 3305 Size: 6160 PstI C_TGCA'G Cuts at: 830 2463 3512 830 Size: 1633 1049 3478 Fragments arranged by size: 3478 1633 1049 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6160 PvuII CAG'CTG Cuts at: 2476 3241 2476 Size: 765 5395 SacII CC_GC'GG Cuts at: 2534 2534 Size: 6160 SalI G'TCGA_C Cuts at: 1936 3502 1936 Size: 1566 4594 SapI GCTCTTCn'nnn_ Cuts at: 2045 2949 2045 Size: 904 5256 SbfI CC_TGCA'GG Cuts at: 3512 3512 Size: 6160 ScaI AGT'ACT Cuts at: 4408 6120 4408 Size: 1712 4448 SfcI C'TryA_G Cuts at: 826 1045 2459 2924 3508 4643 5324 5515 826 Size: 219 1414 465 584 1135 681 191 1471 Fragments arranged by size: 1471 1414 1135 681 584 465 219 191 SfoI GGC'GCC Cuts at: 2192 2192 Size: 6160 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6160 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6160 SmaI CCC'GGG Cuts at: 1872 2201 3256 3487 1872 Size: 329 1055 231 4545 Fragments arranged by size: 4545 1055 329 231 SmlI C'TyrA_G Cuts at: 820 1017 1943 4264 5165 5427 820 Size: 197 926 2321 901 262 1553 Fragments arranged by size: 2321 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3951 493 Size: 3458 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6160 SphI G_CATG'C Cuts at: 1132 3518 1132 Size: 2386 3774 SspI AAT'ATT Cuts at: 5 52 4084 5 Size: 47 4032 2081 Fragments arranged by size: 4032 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6160 StyI C'CwwG_G Cuts at: 513 1065 2004 3222 3599 513 Size: 552 939 1218 377 3074 Fragments arranged by size: 3074 1218 939 552 377 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2675 4187 4204 4357 4542 4987 325 Size: 1255 1095 1512 17 153 185 445 1498 Fragments arranged by size: 1512 1498 1255 1095 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2725 2998 4406 Size: 274 80 33 51 1443 748 273 1408 Cuts at: 4406 6118 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1408 748 274 273 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6120 6152 6120 Size: 32 6128 Tth111I GACn'n_nGTC Cuts at: 1316 2448 3408 1316 Size: 1132 960 4068 Fragments arranged by size: 4068 1132 960 XbaI T'CTAG_A Cuts at: 3313 3496 3313 Size: 183 5977 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6160 XmaI C'CCGG_G Cuts at: 1870 2199 3254 3485 1870 Size: 329 1055 231 4545 Fragments arranged by size: 4545 1055 329 231 XmnI GAAnn'nnTTC Cuts at: 4289 4289 Size: 6160 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3968 276 Size: 53 83 186 936 2434 2468 Fragments arranged by size: 2468 2434 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I EcoICRI EcoNI FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SexAI SfiI SrfI SwaI XcmI