Restriction map for
pFN21AB7405
(Linear) (Six-base) MAPSORT of: KIBB7405.seq Check: 1515 from: 1 to: 1455
KIBB7405.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 628 1455
Size: 628 827
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 186 719 820 1455
Size: 186 533 101 635
Fragments arranged by size:
635 533 186 101
AleI CACnn'nnGTG
Cuts at: 0 1295 1455
Size: 1295 160
AlwNI CAG_nnn'CTG
Cuts at: 0 471 1455
Size: 471 984
ApaI G_GGCC'C
Cuts at: 0 1304 1455
Size: 1304 151
ApaLI G'TGCA_C
Cuts at: 0 1003 1455
Size: 1003 452
ApoI r'AATT_y
Cuts at: 0 1094 1455
Size: 1094 361
AvaI C'yCGr_G
Cuts at: 0 194 319 337 1249 1455
Size: 194 125 18 912 206
Fragments arranged by size:
912 206 194 125 18
BanI G'GyrC_C
Cuts at: 0 185 1455
Size: 185 1270
BanII G_rGCy'C
Cuts at: 0 1304 1455
Size: 1304 151
BbeI G_GCGC'C
Cuts at: 0 189 1455
Size: 189 1266
BbsI GAAGACnn'nnnn_
Cuts at: 0 252 677 1455
Size: 252 425 778
Fragments arranged by size:
778 425 252
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 84 118 1455
Size: 84 34 1337
Fragments arranged by size:
1337 84 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1067 1455
Size: 1067 388
BlpI GC'TnA_GC
Cuts at: 0 247 1229 1286 1455
Size: 247 982 57 169
Fragments arranged by size:
982 247 169 57
Bme1580I G_kGCm'C
Cuts at: 0 1007 1257 1304 1455
Size: 1007 250 47 151
Fragments arranged by size:
1007 250 151 47
BmgBI CAC'GTC
Cuts at: 0 23 1272 1455
Size: 23 1249 183
Fragments arranged by size:
1249 183 23
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 96 1455
Size: 96 1359
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1192 1455
Size: 1192 263
Bpu10I CC'TnA_GC
Cuts at: 0 163 1455
Size: 163 1292
BsaI GGTCTCn'nnnn_
Cuts at: 0 84 895 1455
Size: 84 811 560
Fragments arranged by size:
811 560 84
BsaHI Gr'CG_yC
Cuts at: 0 186 359 1455
Size: 186 173 1096
Fragments arranged by size:
1096 186 173
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 118 148 1455
Size: 118 30 1307
Fragments arranged by size:
1307 118 30
BseYI C'CCAG_C
Cuts at: 0 841 1455
Size: 841 614
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1030 1455
Size: 1030 425
BsiEI CG_ry'CG
Cuts at: 0 569 620 1455
Size: 569 51 835
Fragments arranged by size:
835 569 51
BsiHKAI G_wGCw'C
Cuts at: 0 50 350 1007 1292 1455
Size: 50 300 657 285 163
Fragments arranged by size:
657 300 285 163 50
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1337 1455
Size: 1337 118
Bsp1286I G_dGCh'C
Cuts at: 0 50 350 1007 1257 1292 1304 1455
Size: 50 300 657 250 35 12 151
Fragments arranged by size:
657 300 250 151 50 35 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 628 1455
Size: 628 827
BsrFI r'CCGG_y
Cuts at: 0 26 169 1455
Size: 26 143 1286
Fragments arranged by size:
1286 143 26
BsrGI T'GTAC_A
Cuts at: 0 993 1455
Size: 993 462
BssHII G'CGCG_C
Cuts at: 0 242 531 1455
Size: 242 289 924
Fragments arranged by size:
924 289 242
BstAPI GCAn_nnn'nTGC
Cuts at: 0 23 1455
Size: 23 1432
BstXI CCAn_nnnn'nTGG
Cuts at: 0 291 1455
Size: 291 1164
Bsu36I CC'TnA_GG
Cuts at: 0 509 1061 1455
Size: 509 552 394
Fragments arranged by size:
552 509 394
BtgI C'CryG_G
Cuts at: 0 526 755 1395 1455
Size: 526 229 640 60
Fragments arranged by size:
640 526 229 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 87 162 1455
Size: 87 75 1293
Fragments arranged by size:
1293 87 75
BtsI GCAGTG_nn'
Cuts at: 0 832 996 1255 1455
Size: 832 164 259 200
Fragments arranged by size:
832 259 200 164
ClaI AT'CG_AT
Cuts at: 0 329 1455
Size: 329 1126
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1337 1372 1455
Size: 1337 35 83
Fragments arranged by size:
1337 83 35
DraIII CAC_nnn'GTG
Cuts at: 0 1131 1455
Size: 1131 324
EaeI y'GGCC_r
Cuts at: 0 566 773 1455
Size: 566 207 682
Fragments arranged by size:
682 566 207
EagI C'GGCC_G
Cuts at: 0 566 1455
Size: 566 889
EarI CTCTTCn'nnn_
Cuts at: 0 40 796 944 1335 1455
Size: 40 756 148 391 120
Fragments arranged by size:
756 391 148 120 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 85 490 553 1455
Size: 85 405 63 902
Fragments arranged by size:
902 405 85 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 186 719 820 1192 1455
Size: 186 533 101 372 263
Fragments arranged by size:
533 372 263 186 101
EcoO109I rG'GnC_Cy
Cuts at: 0 505 522 584 810 1301 1455
Size: 505 17 62 226 491 154
Fragments arranged by size:
505 491 226 154 62 17
EcoRV GAT'ATC
Cuts at: 0 1075 1455
Size: 1075 380
HaeII r_GCGC'y
Cuts at: 0 170 189 209 497 1455
Size: 170 19 20 288 958
Fragments arranged by size:
958 288 170 20 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 130 162 679 711 1455
Size: 130 32 517 32 744
Fragments arranged by size:
744 517 130 32 32
HincII GTy'rAC
Cuts at: 0 378 942 1440 1455
Size: 378 564 498 15
Fragments arranged by size:
564 498 378 15
KasI G'GCGC_C
Cuts at: 0 185 1455
Size: 185 1270
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 161 1455
Size: 140 21 1294
Fragments arranged by size:
1294 140 21
MslI CAynn'nnrTG
Cuts at: 0 43 289 1274 1295 1455
Size: 43 246 985 21 160
Fragments arranged by size:
985 246 160 43 21
MspA1I CmG'CkG
Cuts at: 0 462 471 528 907 1236 1455
Size: 462 9 57 379 329 219
Fragments arranged by size:
462 379 329 219 57 9
NaeI GCC'GGC
Cuts at: 0 28 171 1455
Size: 28 143 1284
Fragments arranged by size:
1284 143 28
NarI GG'CG_CC
Cuts at: 0 186 1455
Size: 186 1269
NgoMIV G'CCGG_C
Cuts at: 0 26 169 1455
Size: 26 143 1286
Fragments arranged by size:
1286 143 26
NspI r_CATG'y
Cuts at: 0 132 733 1455
Size: 132 601 722
Fragments arranged by size:
722 601 132
PasI CC'CwG_GG
Cuts at: 0 747 1455
Size: 747 708
PfoI T'CCnGG_A
Cuts at: 0 401 1455
Size: 401 1054
PpuMI rG'GwC_Cy
Cuts at: 0 522 584 810 1455
Size: 522 62 226 645
Fragments arranged by size:
645 522 226 62
PspOMI G'GGCC_C
Cuts at: 0 1300 1455
Size: 1300 155
PstI C_TGCA'G
Cuts at: 0 458 1455
Size: 458 997
PvuII CAG'CTG
Cuts at: 0 471 1236 1455
Size: 471 765 219
Fragments arranged by size:
765 471 219
SacII CC_GC'GG
Cuts at: 0 529 1455
Size: 529 926
SapI GCTCTTCn'nnn_
Cuts at: 0 40 944 1455
Size: 40 904 511
Fragments arranged by size:
904 511 40
SfcI C'TryA_G
Cuts at: 0 454 919 1455
Size: 454 465 536
Fragments arranged by size:
536 465 454
SfoI GGC'GCC
Cuts at: 0 187 1455
Size: 187 1268
SmaI CCC'GGG
Cuts at: 0 196 1251 1455
Size: 196 1055 204
Fragments arranged by size:
1055 204 196
StyI C'CwwG_G
Cuts at: 0 1217 1455
Size: 1217 238
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 670 1455
Size: 670 785
TatI w'GTAC_w
Cuts at: 0 720 993 1455
Size: 720 273 462
Fragments arranged by size:
720 462 273
Tth111I GACn'n_nGTC
Cuts at: 0 443 1403 1455
Size: 443 960 52
Fragments arranged by size:
960 443 52
XbaI T'CTAG_A
Cuts at: 0 1308 1455
Size: 1308 147
XmaI C'CCGG_G
Cuts at: 0 194 1249 1455
Size: 194 1055 206
Fragments arranged by size:
1055 206 194
Enzymes that do cut:
AarI AcuI AleI AlwNI ApaI ApaLI ApoI AvaI
BanI BanII BbeI BbsI BcgI BciVI BlpI Bme1580I
BmgBI BplI BpmI Bpu10I BsaI BsaHI BsaXI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BsrGI
BssHII BstAPI BstXI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraIII EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRV HaeII Hin4I HincII KasI MmeI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PfoI PpuMI PspOMI
PstI PvuII SacII SapI SfcI SfoI SmaI StyI
TaqII TatI Tth111I XbaI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BclI BfrBI BglI BglII BmrI BmtI
BpuEI BsaAI BsaBI BsaWI BseRI BsiWI BsmI BspEI
BspHI BsrBI BsrDI BssSI BstBI BstEII BstYI BstZ17I
DraI DrdI EcoICRI EcoNI EcoRI FalI FseI FspI
FspAI HindIII HpaI KpnI MfeI MluI MscI NcoI
NdeI NheI NotI NruI NsiI PacI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SalI SanDI SbfI ScaI SexAI SfiI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI XcmI XhoI XmnI ZraI