Restriction map for
pFN21AB7400
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccII AccIII AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbvCI BciVI BclI BcnI
BfrI BglI BlnI BlpI BpmI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmFI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MamI MfeI MluI MroI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 223 GTCTAC GT^[AC][GT]AC
AclI 483 AACGTT AA^CGTT
AlwNI 20 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 870 CCTAAGG CC^T[ACGT]AGG
AspHI 631 GTGCTC G[AT]GC[AT]^C
BalI 368 TGGCCA TGG^CCA
BbsI 259 GAAGAC GAAGAC(2/6)
BcgI 125 GCACCCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 784 AGATCT A^GATCT
BmyI 631 GTGCTC G[AGT]GC[ACT]^C
BpuAI 259 GAAGAC GAAGAC(2/6)
BsaAI 364 TACGTG [CT]AC^GT[GA]
BsiHKAI 631 GTGCTC G[AT]GC[AT]^C
BsmAI 436 GTCTC GTCTC(1/5)
BsmI 62 GAATGC GAATGC(1/-1)
Bsp1286I 631 GTGCTC G[AGT]GC[ACT]^C
BspHI 857 TCATGA T^CATGA
BsrDI 422 CATTGC GCAATG(2/0)
Bsu36I 870 CCTAAGG CC^T[ACGT]AGG
Cfr13I 72 GGGCC G^G[ACGT]CC
DsaI 347 CCGTGG C^C[GA][CT]GG
EaeI 368 TGGCCA [CT]^GGCC[GA]
Eco81I 870 CCTAAGG CC^T[ACGT]AGG
FauI 625 CCCGC CCCGC(4/6)
MluNI 368 TGGCCA TGG^CCA
MscI 368 TGGCCA TGG^CCA
MslI 165 CACACCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 67 CAGCTG C[AC]G^C[GT]G
NlaIV 754 GGCTCC GG[ACGT]^[ACGT]CC
NspI 715 ACATGC [GA]CATG^[CT]
PleI 151 GACTC GAGTC(4/5)
PshAI 732 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 483 AACGTT AA^CGTT
PstI 308 CTGCAG CTGCA^G
PvuII 67 CAGCTG CAG^CTG
RcaI 857 TCATGA T^CATGA
Sau96I 72 GGGCC G^G[ACGT]CC
SmlI 672 CTTGAG C^T[CT][GA]AG
TfiI 852 GAATC G^A[AT]TC
Tsp509I 585 AATT ^AATT
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# TWO CUT ENZYMES ( 20 enzymes) #
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AfaI 99 GTAC GT^AC
594 GTAC
BfaI 241 CTAG C^TAG
788 CTAG
BsiYI 75 CCTCTTCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
360 CCGCTACGTGG
BslI 75 CCTCTTCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
360 CCGCTACGTGG
BspMI 247 ACCTGC ACCTGC(4/8)
335 ACCTGC
BsrI 184 ACTGG ACTGG(1/-1)
665 CCAGT
BstF5I 636 CATCC GGATG(2/0)
882 GGATG
DdeI 26 CTGAG C^T[ACGT]AG
871 CTAAG
EarI 76 CTCTTC CTCTTC(1/4)
462 CTCTTC
EcoT14I 545 CCTTGG C^C[AT][AT]GG
689 CCAAGG
FokI 636 CATCC GGATG(9/13)
882 GGATG
HinfI 151 GACTC G^A[ACGT]TC
852 GAATC
Ksp632I 76 CTCTTC CTCTTC(1/4)
462 CTCTTC
MaeI 241 CTAG C^TAG
788 CTAG
MaeII 365 ACGT A^CGT
484 ACGT
MseI 458 TTAA T^TAA
494 TTAA
RsaI 99 GTAC GT^AC
594 GTAC
SfaNI 251 GCATC GCATC(5/9)
269 GCATC
StyI 545 CCTTGG C^C[AT][AT]GG
689 CCAAGG
Tru9I 458 TTAA T^TAA
494 TTAA
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AluI AlwI BbvI BsaJI
BseRI BssKI BstNI BstYI Cac8I
DpnII EcoRII Fnu4HI HaeIII HphI
ItaI MaeIII MboI MboII MflI
MnlI MvaI MwoI NdeII NlaIII
Sau3AI ScrFI SfcI TseI Tsp45I
TspRI XhoII