(Circular) (Six-base) MAPSORT of: pFN21AB7400.seq Check: 9614 from: 1 to: 5620 pFN21AB7400 5620 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3430 278 Size: 53 83 186 936 1894 2468 Fragments arranged by size: 2468 1894 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2229 2963 1937 Size: 292 734 4594 Fragments arranged by size: 4594 734 292 Acc65I G'GTAC_C Cuts at: 2941 2941 Size: 5620 AclI AA'CG_TT Cuts at: 1563 2489 3747 4120 1563 Size: 926 1258 373 3063 Fragments arranged by size: 3063 1258 926 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2283 3683 5067 5335 1632 Size: 651 1400 1384 268 1917 Fragments arranged by size: 1917 1400 1384 651 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5423 AflIII A'CryG_T Cuts at: 1203 4519 5496 1203 Size: 3316 977 1327 Fragments arranged by size: 3316 1327 977 AgeI A'CCGG_T Cuts at: 4451 4451 Size: 5620 AhdI GACnn_n'nnGTC Cuts at: 4349 4349 Size: 5620 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5620 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5620 AlwNI CAG_nnn'CTG Cuts at: 2030 3363 4935 2030 Size: 1333 1572 2715 Fragments arranged by size: 2715 1572 1333 ApaLI G'TGCA_C Cuts at: 3677 4833 3677 Size: 1156 4464 ApoI r'AATT_y Cuts at: 1493 2929 3155 3307 5416 1493 Size: 1436 226 152 2109 1697 Fragments arranged by size: 2109 1697 1436 226 152 AseI AT'TA_AT Cuts at: 160 1032 4174 160 Size: 872 3142 1606 Fragments arranged by size: 3142 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5620 AvaI C'yCGr_G Cuts at: 1870 1943 2945 1870 Size: 73 1002 4545 Fragments arranged by size: 4545 1002 73 BamHI G'GATC_C Cuts at: 2950 3317 2950 Size: 367 5253 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2941 4396 618 Size: 325 217 627 1154 1455 1842 Fragments arranged by size: 1842 1455 1154 627 325 217 BanII G_rGCy'C Cuts at: 1391 2939 1391 Size: 1548 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2271 928 Size: 971 372 4277 Fragments arranged by size: 4277 971 372 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2119 2153 3811 3845 1903 Size: 34 50 34 98 34 1658 34 3678 Fragments arranged by size: 3678 1658 98 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3512 4722 3512 Size: 1210 4410 BclI T'GATC_A Cuts at: 1548 1548 Size: 5620 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4231 136 Size: 107 122 71 631 605 2559 1525 Fragments arranged by size: 2559 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2789 5615 2789 Size: 2826 2794 BlpI GC'TnA_GC Cuts at: 3037 3037 Size: 5620 Bme1580I G_kGCm'C Cuts at: 1790 3681 4837 1790 Size: 1891 1156 2573 Fragments arranged by size: 2573 1891 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5620 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2912 3351 4309 Size: 416 53 83 399 24 1495 439 958 Cuts at: 4309 442 Size: 1753 Fragments arranged by size: 1753 1495 958 439 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5620 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4280 1239 Size: 3041 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2698 3745 4610 4908 2698 Size: 1047 865 298 3410 Fragments arranged by size: 3410 1047 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4283 882 Size: 617 2784 2219 Fragments arranged by size: 2784 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2371 3411 493 Size: 711 1167 1040 2702 Fragments arranged by size: 2702 1167 1040 711 BsaBI GATnn'nnATC Cuts at: 5614 5614 Size: 5620 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3427 3809 4444 275 Size: 53 83 186 936 1894 382 635 1451 Fragments arranged by size: 1894 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4053 4451 4725 4872 5283 4053 Size: 398 274 147 411 4390 Fragments arranged by size: 4390 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2744 2774 1178 Size: 30 165 30 1341 30 4024 Fragments arranged by size: 4024 1341 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2026 2677 2680 2716 1186 Size: 840 651 3 36 4090 Fragments arranged by size: 4090 840 651 36 3 BseYI C'CCAG_C Cuts at: 2044 2074 4823 5319 2044 Size: 30 2749 496 2345 Fragments arranged by size: 2749 2345 496 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5047 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3070 3831 4859 1540 Size: 275 186 1069 761 1028 2301 Fragments arranged by size: 2301 1069 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2640 3681 3766 4837 2640 Size: 1041 85 1071 3423 Fragments arranged by size: 3423 1071 1041 85 BsmI GAATG_Cn' Cuts at: 2073 3131 3224 2073 Size: 1058 93 4469 Fragments arranged by size: 4469 1058 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2640 2939 3681 3766 4837 1391 Size: 399 850 299 742 85 1071 2174 Fragments arranged by size: 2174 1071 850 742 399 299 85 BspEI T'CCGG_A Cuts at: 5283 5283 Size: 5620 BspHI T'CATG_A Cuts at: 2862 3509 5239 2862 Size: 647 1730 3243 Fragments arranged by size: 3243 1730 647 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2261 2349 2975 844 Size: 1417 88 626 3489 Fragments arranged by size: 3489 1417 626 88 BsrBI CCG'CTC Cuts at: 1394 1823 3507 1394 Size: 429 1684 3507 Fragments arranged by size: 3507 1684 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2426 4115 4289 65 Size: 1183 1178 1689 174 1396 Fragments arranged by size: 1689 1396 1183 1178 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4264 4451 1700 Size: 110 2454 187 2869 Fragments arranged by size: 2869 2454 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5620 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5620 BssSI C'ACGA_G Cuts at: 3680 4692 3680 Size: 1012 4608 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5620 BstBI TT'CG_AA Cuts at: 4424 4424 Size: 5620 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5620 BstYI r'GATC_y Cuts at: 1972 2209 2241 2682 2789 2950 3317 3702 3719 Size: 237 32 441 107 161 367 385 17 Cuts at: 3719 5171 5257 5269 5615 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 441 385 367 346 237 161 107 86 32 17 12 Bsu36I CC'TnA_GG Cuts at: 2876 2876 Size: 5620 BtgI C'CryG_G Cuts at: 513 1065 2352 513 Size: 552 1287 3781 Fragments arranged by size: 3781 1287 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4881 Fragments arranged by size: 4881 683 56 BtsI GCAGTG_nn' Cuts at: 2640 3136 3930 3950 2640 Size: 496 794 20 4310 Fragments arranged by size: 4310 794 496 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5620 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5585 DraI TTT'AAA Cuts at: 2924 2924 Size: 5620 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4627 809 Size: 507 3311 1802 Fragments arranged by size: 3311 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2373 3067 3956 Size: 54 1425 50 275 18 543 694 889 Cuts at: 3956 5585 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 694 543 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3067 1537 Size: 275 1255 4090 Fragments arranged by size: 4090 1255 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2087 2473 3550 1353 Size: 734 386 1077 3423 Fragments arranged by size: 3423 1077 734 386 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4207 4581 4727 387 Size: 1064 138 2618 374 146 1280 Fragments arranged by size: 2618 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2283 3683 4280 5067 5335 1239 Size: 393 651 1400 597 787 268 1524 Fragments arranged by size: 1524 1400 787 651 597 393 268 EcoRI G'AATT_C Cuts at: 2929 3307 2929 Size: 378 5242 FspI TGC'GCA Cuts at: 4126 4126 Size: 5620 HaeII r_GCGC'y Cuts at: 1678 4767 1678 Size: 3089 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2002 2034 4261 4293 4335 Size: 32 135 32 430 32 2227 32 42 Cuts at: 4335 4367 1373 Size: 32 2626 Fragments arranged by size: 2626 2227 430 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2964 3210 669 Size: 988 281 1026 246 3079 Fragments arranged by size: 3079 1026 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5620 HpaI GTT'AAC Cuts at: 3210 3210 Size: 5620 KpnI G_GTAC'C Cuts at: 2945 2945 Size: 5620 MfeI C'AATT_G Cuts at: 3219 3219 Size: 5620 MluI A'CGCG_T Cuts at: 5496 5496 Size: 5620 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4734 4918 1305 Size: 3429 184 2007 Fragments arranged by size: 3429 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2375 10 Size: 54 1425 343 543 3255 Fragments arranged by size: 3255 1425 543 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2174 3578 3937 4096 518 Size: 648 1008 1404 359 159 2042 Fragments arranged by size: 2042 1404 1008 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2074 3036 3713 4861 5106 1606 Size: 468 962 677 1148 245 2120 Fragments arranged by size: 2120 1148 962 677 468 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5068 NdeI CA'TA_TG Cuts at: 387 387 Size: 5620 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5620 NotI GC'GGCC_GC Cuts at: 3067 3067 Size: 5620 NspI r_CATG'y Cuts at: 1132 1229 2724 2978 4523 1132 Size: 97 1495 254 1545 2229 Fragments arranged by size: 2229 1545 1495 254 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5620 PciI A'CATG_T Cuts at: 4519 4519 Size: 5620 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5620 PmeI GTTT'AAAC Cuts at: 2924 2924 Size: 5620 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3689 3721 5202 5234 3689 Size: 32 1481 32 4075 Fragments arranged by size: 4075 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2741 1613 Size: 1128 4492 PsiI TTA'TAA Cuts at: 3190 3190 Size: 5620 PstI C_TGCA'G Cuts at: 830 2317 2972 830 Size: 1487 655 3478 Fragments arranged by size: 3478 1487 655 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5620 PvuII CAG'CTG Cuts at: 2074 2074 Size: 5620 SalI G'TCGA_C Cuts at: 1936 2962 1936 Size: 1026 4594 SbfI CC_TGCA'GG Cuts at: 2972 2972 Size: 5620 ScaI AGT'ACT Cuts at: 3868 5580 3868 Size: 1712 3908 SfcI C'TryA_G Cuts at: 826 1045 2313 2326 2869 2968 4103 4784 4975 Size: 219 1268 13 543 99 1135 681 191 Cuts at: 4975 826 Size: 1471 Fragments arranged by size: 1471 1268 1135 681 543 219 191 99 13 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5620 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5620 SmaI CCC'GGG Cuts at: 1872 2947 1872 Size: 1075 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2677 3724 4625 4887 820 Size: 197 926 734 1047 901 262 1553 Fragments arranged by size: 1553 1047 926 901 734 262 197 SnaBI TAC'GTA Cuts at: 493 3411 493 Size: 2918 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5620 SphI G_CATG'C Cuts at: 1132 2978 1132 Size: 1846 3774 SspI AAT'ATT Cuts at: 5 52 3544 5 Size: 47 3492 2081 Fragments arranged by size: 3492 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5620 StyI C'CwwG_G Cuts at: 513 1065 2550 2694 3059 513 Size: 552 1485 144 365 3074 Fragments arranged by size: 3074 1485 552 365 144 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2188 3647 3664 3817 4002 4447 325 Size: 1255 608 1459 17 153 185 445 1498 Fragments arranged by size: 1498 1459 1255 608 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2103 2598 3866 Size: 274 80 33 51 1443 126 495 1268 Cuts at: 3866 5578 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1268 495 274 138 126 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5580 5612 5580 Size: 32 5588 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5620 XbaI T'CTAG_A Cuts at: 2956 2956 Size: 5620 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5620 XmaI C'CCGG_G Cuts at: 1870 2945 1870 Size: 1075 4545 XmnI GAAnn'nnTTC Cuts at: 3749 3749 Size: 5620 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3428 276 Size: 53 83 186 936 1894 2468 Fragments arranged by size: 2468 1894 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI