(Circular) (Six-base) MAPSORT of: pFN21AB7391.seq Check: 8897 from: 1 to: 5398 pFN21AB7391 5398 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2483 2483 Size: 5398 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3208 278 Size: 53 83 186 936 1672 2468 Fragments arranged by size: 2468 1672 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2741 1937 Size: 804 4594 Acc65I G'GTAC_C Cuts at: 2719 2719 Size: 5398 AclI AA'CG_TT Cuts at: 1563 3525 3898 1563 Size: 1962 373 3063 Fragments arranged by size: 3063 1962 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2051 3461 4845 5113 1632 Size: 419 1410 1384 268 1917 Fragments arranged by size: 1917 1410 1384 419 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5201 AflIII A'CryG_T Cuts at: 1203 2285 4297 5274 1203 Size: 1082 2012 977 1327 Fragments arranged by size: 2012 1327 1082 977 AgeI A'CCGG_T Cuts at: 4229 4229 Size: 5398 AhdI GACnn_n'nnGTC Cuts at: 4127 4127 Size: 5398 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5398 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5398 AlwNI CAG_nnn'CTG Cuts at: 2359 3141 4713 2359 Size: 782 1572 3044 Fragments arranged by size: 3044 1572 782 ApaLI G'TGCA_C Cuts at: 3455 4611 3455 Size: 1156 4242 ApoI r'AATT_y Cuts at: 1493 2707 2933 3085 5194 1493 Size: 1214 226 152 2109 1697 Fragments arranged by size: 2109 1697 1214 226 152 AseI AT'TA_AT Cuts at: 160 1032 3952 160 Size: 872 2920 1606 Fragments arranged by size: 2920 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5398 AvaI C'yCGr_G Cuts at: 1870 1943 2723 1870 Size: 73 780 4545 Fragments arranged by size: 4545 780 73 AvrII C'CTAG_G Cuts at: 2234 2234 Size: 5398 BamHI G'GATC_C Cuts at: 2728 3095 2728 Size: 367 5031 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2692 2719 4174 618 Size: 325 217 627 905 27 1455 1842 Fragments arranged by size: 1842 1455 905 627 325 217 27 BanII G_rGCy'C Cuts at: 1391 2717 1391 Size: 1326 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4427 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2461 2495 3589 3623 1903 Size: 34 524 34 1094 34 3678 Fragments arranged by size: 3678 1094 524 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3290 4500 3290 Size: 1210 4188 BclI T'GATC_A Cuts at: 1548 1548 Size: 5398 BfrBI ATG'CAT Cuts at: 2426 2426 Size: 5398 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4009 136 Size: 107 122 71 631 605 2337 1525 Fragments arranged by size: 2337 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5393 5393 Size: 5398 BlpI GC'TnA_GC Cuts at: 2815 2815 Size: 5398 Bme1580I G_kGCm'C Cuts at: 1790 2697 3459 4615 1790 Size: 907 762 1156 2573 Fragments arranged by size: 2573 1156 907 762 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5398 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3129 4087 442 Size: 416 53 83 399 24 1712 958 1753 Fragments arranged by size: 1753 1712 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5398 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2595 4058 1239 Size: 1356 1463 2579 Fragments arranged by size: 2579 1463 1356 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3523 4388 4686 3523 Size: 865 298 4235 Fragments arranged by size: 4235 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2058 4061 882 Size: 617 559 2003 2219 Fragments arranged by size: 2219 2003 617 559 BsaAI yAC'GTr Cuts at: 493 1204 3189 493 Size: 711 1985 2702 Fragments arranged by size: 2702 1985 711 BsaBI GATnn'nnATC Cuts at: 5392 5392 Size: 5398 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3205 3587 4222 275 Size: 53 83 186 936 1672 382 635 1451 Fragments arranged by size: 1672 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3831 4229 4503 4650 5061 3831 Size: 398 274 147 411 4168 Fragments arranged by size: 4168 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5173 Fragments arranged by size: 5173 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2420 1186 Size: 1234 4164 BseYI C'CCAG_C Cuts at: 4601 5097 4601 Size: 496 4902 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2158 2293 1163 Size: 573 422 135 4268 Fragments arranged by size: 4268 573 422 135 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2848 3609 4637 1540 Size: 275 186 847 761 1028 2301 Fragments arranged by size: 2301 1028 847 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3459 3544 4615 3459 Size: 85 1071 4242 Fragments arranged by size: 4242 1071 85 BsmI GAATG_Cn' Cuts at: 2105 2909 3002 2105 Size: 804 93 4501 Fragments arranged by size: 4501 804 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2697 2717 3459 3544 4615 1391 Size: 399 907 20 742 85 1071 2174 Fragments arranged by size: 2174 1071 907 742 399 85 20 BspEI T'CCGG_A Cuts at: 5061 5061 Size: 5398 BspHI T'CATG_A Cuts at: 3287 5017 3287 Size: 1730 3668 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2483 2753 844 Size: 1639 270 3489 Fragments arranged by size: 3489 1639 270 BsrBI CCG'CTC Cuts at: 1394 1823 3285 1394 Size: 429 1462 3507 Fragments arranged by size: 3507 1462 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3893 4067 65 Size: 1183 2645 174 1396 Fragments arranged by size: 2645 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2459 4042 4229 1700 Size: 110 649 1583 187 2869 Fragments arranged by size: 2869 1583 649 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5398 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5398 BssSI C'ACGA_G Cuts at: 3458 4470 3458 Size: 1012 4386 BstAPI GCAn_nnn'nTGC Cuts at: 794 2042 794 Size: 1248 4150 BstBI TT'CG_AA Cuts at: 4202 4202 Size: 5398 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5398 BstYI r'GATC_y Cuts at: 1972 2193 2685 2728 3095 3480 3497 4949 5035 Size: 221 492 43 367 385 17 1452 86 Cuts at: 5035 5047 5393 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 492 385 367 346 221 86 43 17 12 Bsu36I CC'TnA_GG Cuts at: 2211 2211 Size: 5398 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4846 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4659 Fragments arranged by size: 4659 683 56 BtsI GCAGTG_nn' Cuts at: 2914 3708 3728 2914 Size: 794 20 4584 Fragments arranged by size: 4584 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5398 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5363 DraI TTT'AAA Cuts at: 2702 2702 Size: 5398 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2665 4405 809 Size: 507 1349 1740 1802 Fragments arranged by size: 1802 1740 1349 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2467 2602 2845 Size: 54 1425 50 275 18 637 135 243 Cuts at: 2845 3734 5363 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 637 275 243 135 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2845 1537 Size: 275 1033 4090 Fragments arranged by size: 4090 1033 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3328 1353 Size: 1975 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3985 4359 4505 387 Size: 1064 138 2396 374 146 1280 Fragments arranged by size: 2396 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2051 2595 3461 4058 4845 5113 1239 Size: 393 419 544 866 597 787 268 1524 Fragments arranged by size: 1524 866 787 597 544 419 393 268 EcoRI G'AATT_C Cuts at: 2707 3085 2707 Size: 378 5020 FspI TGC'GCA Cuts at: 3904 3904 Size: 5398 HaeII r_GCGC'y Cuts at: 1678 4545 1678 Size: 2867 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4039 4071 4113 4145 1373 Size: 32 135 32 2467 32 42 32 2626 Fragments arranged by size: 2626 2467 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2742 2988 669 Size: 988 281 804 246 3079 Fragments arranged by size: 3079 988 804 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5398 HpaI GTT'AAC Cuts at: 2988 2988 Size: 5398 KpnI G_GTAC'C Cuts at: 2723 2723 Size: 5398 MfeI C'AATT_G Cuts at: 2997 2997 Size: 5398 MluI A'CGCG_T Cuts at: 5274 5274 Size: 5398 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2171 4512 4696 1305 Size: 866 2341 184 2007 Fragments arranged by size: 2341 2007 866 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3576 Fragments arranged by size: 3576 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3356 3715 3874 518 Size: 648 2190 359 159 2042 Fragments arranged by size: 2190 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2387 2814 3491 4639 4884 1606 Size: 781 427 677 1148 245 2120 Fragments arranged by size: 2120 1148 781 677 427 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4846 NdeI CA'TA_TG Cuts at: 387 387 Size: 5398 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5398 NotI GC'GGCC_GC Cuts at: 2845 2845 Size: 5398 NsiI A_TGCA'T Cuts at: 2428 2428 Size: 5398 NspI r_CATG'y Cuts at: 1132 1229 2087 2289 2756 4301 1132 Size: 97 858 202 467 1545 2229 Fragments arranged by size: 2229 1545 858 467 202 97 PasI CC'CwG_GG Cuts at: 1348 2437 1348 Size: 1089 4309 PciI A'CATG_T Cuts at: 2285 4297 2285 Size: 2012 3386 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5398 PfoI T'CCnGG_A Cuts at: 2063 2063 Size: 5398 PmeI GTTT'AAAC Cuts at: 2702 2702 Size: 5398 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3467 3499 4980 5012 3467 Size: 32 1481 32 3853 Fragments arranged by size: 3853 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5398 PsiI TTA'TAA Cuts at: 2968 2968 Size: 5398 PstI C_TGCA'G Cuts at: 830 2077 2456 2750 830 Size: 1247 379 294 3478 Fragments arranged by size: 3478 1247 379 294 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5398 SalI G'TCGA_C Cuts at: 1936 2740 1936 Size: 804 4594 SbfI CC_TGCA'GG Cuts at: 2750 2750 Size: 5398 ScaI AGT'ACT Cuts at: 3646 5358 3646 Size: 1712 3686 SfcI C'TryA_G Cuts at: 826 1045 2073 2452 2746 3881 4562 4753 826 Size: 219 1028 379 294 1135 681 191 1471 Fragments arranged by size: 1471 1135 1028 681 379 294 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5398 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5398 SmaI CCC'GGG Cuts at: 1872 2725 1872 Size: 853 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3502 4403 4665 820 Size: 197 926 1559 901 262 1553 Fragments arranged by size: 1559 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3189 493 Size: 2696 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5398 SphI G_CATG'C Cuts at: 1132 2756 1132 Size: 1624 3774 SspI AAT'ATT Cuts at: 5 52 3322 5 Size: 47 3270 2081 Fragments arranged by size: 3270 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5398 StyI C'CwwG_G Cuts at: 513 1065 2234 2837 513 Size: 552 1169 603 3074 Fragments arranged by size: 3074 1169 603 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3425 3442 3595 3780 4225 325 Size: 1255 1845 17 153 185 445 1498 Fragments arranged by size: 1845 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3644 5356 96 Size: 274 80 33 51 1443 1667 1712 138 Fragments arranged by size: 1712 1667 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5358 5390 5358 Size: 32 5366 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5398 XbaI T'CTAG_A Cuts at: 2734 2734 Size: 5398 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5398 XmaI C'CCGG_G Cuts at: 1870 2723 1870 Size: 853 4545 XmnI GAAnn'nnTTC Cuts at: 3527 3527 Size: 5398 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3206 276 Size: 53 83 186 936 1672 2468 Fragments arranged by size: 2468 1672 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI