Restriction map for
pFN21AB7390
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AlwI AocI
ApaI AscI AseI AsnI Asp718I
AviII AvrII BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglII BlnI
BpmI BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII Bst1107I
BstAPI BstEII BstPI BstYI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
EagI EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HindIII HpaI
KasI KpnI KspI MaeI MaeII
MamI MfeI MflI MluI MroI
MslI MspA1I MunI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI XbaI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccII 901 CGCG CG^CG
AcsI 505 AAATTC [GA]^AATT[CT]
AcyI 930 GACGCC G[GA]^CG[CT]C
AfaI 315 GTAC GT^AC
AhdI 450 GACACTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 67 GTGCAC G^TGCAC
Aor51HI 365 AGCGCT AGC^GCT
ApaLI 67 GTGCAC G^TGCAC
ApoI 505 AAATTC [GA]^AATT[CT]
Asp700I 174 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 450 GACACTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 98 TGGCCA TGG^CCA
BglI 645 GCCCACACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 930 GACGCC G[GA]^CG[CT]C
BsmBI 928 GAGACG CGTCTC(1/5)
BsmI 164 GAATGC GAATGC(1/-1)
BsrFI 407 ACCGGC [GA]^CCGG[CT]
BssSI 292 CACGAG CACGAG(-5/-1)
BstBI 161 TTCGAA TT^CGAA
BstF5I 122 GGATG GGATG(2/0)
BstUI 901 CGCG CG^CG
Cfr10I 407 ACCGGC [GA]^CCGG[CT]
DrdI 27 GACCGTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 450 GACACTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 365 AGCGCT AGC^GCT
FauI 119 GCGGG CCCGC(4/6)
FokI 122 GGATG GGATG(9/13)
HgaI 930 GACGC GACGC(5/10)
Hin1I 930 GACGCC G[GA]^CG[CT]C
HincII 474 GTTGAC GT[CT]^[GA]AC
HindII 474 GTTGAC GT[CT]^[GA]AC
MluNI 98 TGGCCA TGG^CCA
MscI 98 TGGCCA TGG^CCA
MseI 568 TTAA T^TAA
MvnI 901 CGCG CG^CG
NruI 900 TCGCGA TCG^CGA
NspV 161 TTCGAA TT^CGAA
PflMI 261 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 489 CTGCAG CTGCA^G
RsaI 315 GTAC GT^AC
SfcI 489 CTGCAG C^T[GA][CT]AG
SfuI 161 TTCGAA TT^CGAA
TaqI 162 TCGA T^CGA
Tru9I 568 TTAA T^TAA
Tsp509I 506 AATT ^AATT
TthHB8I 162 TCGA T^CGA
Van91I 261 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 174 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AlwNI 262 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
760 CAGGTCCTG
AvaI 751 CCCGAG C^[CT]CG[GA]G
910 CCCGAG
BanI 416 GGCACC G^G[CT][GA]CC
943 GGCACC
BlpI 87 GCTGAGC GC^T[ACGT]AGC
246 GCTCAGC
Bpu1102I 87 GCTGAGC GC^T[ACGT]AGC
246 GCTCAGC
BsoBI 751 CCCGAG C^[CT]CG[GA]G
910 CCCGAG
BspMI 109 ACCTGC ACCTGC(4/8)
759 GCAGGT
BstXI 866 CCAGGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
947 CCAATGAGATGG
CelII 87 GCTGAGC GC^T[ACGT]AGC
246 GCTCAGC
DpnII 144 GATC ^GATC
517 GATC
DraII 761 AGGTCCT [GA]G^G[ACGT]CC[CT]
868 AGGTCCC
DsaI 29 CCGTGG C^C[GA][CT]GG
938 CCGTGG
EaeI 98 TGGCCA [CT]^GGCC[GA]
350 CGGCCA
EcoO109I 761 AGGTCCT [GA]G^G[ACGT]CC[CT]
868 AGGTCCC
HapII 408 CCGG C^CGG
843 CCGG
HinfI 34 GAGTC G^A[ACGT]TC
582 GACTC
HpaII 408 CCGG C^CGG
843 CCGG
MaeIII 404 GTCAC ^GT[ACGT]AC
780 GTCAC
MboI 144 GATC ^GATC
517 GATC
MspI 408 CCGG C^CGG
843 CCGG
NdeII 144 GATC ^GATC
517 GATC
PleI 582 GACTC GAGTC(4/5)
34 GAGTC
PpuMI 761 AGGTCCT [GA]G^G[AT]CC[CT]
868 AGGTCCC
SacI 90 GAGCTC GAGCT^C
621 GAGCTC
Sau3AI 144 GATC ^GATC
517 GATC
SexAI 80 ACCTGGT A^CC[AT]GGT
865 ACCAGGT
Tsp45I 404 GTCAC ^GT[GC]AC
780 GTCAC
XcmI 242 CCACGCTCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
863 CCACCAGGTCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AluI AspHI AspI AvaII
BanII BbvI BmyI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstNI Cac8I
CfoI Cfr13I DdeI EarI EcoRII
Fnu4HI HaeII HaeIII HhaI HinP1I
HphI ItaI Ksp632I MboII MnlI
MvaI MwoI NlaIII NlaIV PflFI
Sau96I ScrFI SfaNI TseI TspRI
Tth111I