(Circular) (Six-base) MAPSORT of: pFN21AB7390.seq Check: 3681 from: 1 to: 5668 pFN21AB7390 5668 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2123 2123 Size: 5668 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3478 278 Size: 53 83 186 936 1942 2468 Fragments arranged by size: 2468 1942 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3011 1937 Size: 1074 4594 Acc65I G'GTAC_C Cuts at: 2989 2989 Size: 5668 AclI AA'CG_TT Cuts at: 1563 3795 4168 1563 Size: 2232 373 3063 Fragments arranged by size: 3063 2232 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2218 2515 2761 3731 5115 5383 1632 Size: 586 297 246 970 1384 268 1917 Fragments arranged by size: 1917 1384 970 586 297 268 246 AfeI AGC'GCT Cuts at: 2372 2372 Size: 5668 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5471 AflIII A'CryG_T Cuts at: 1203 4567 5544 1203 Size: 3364 977 1327 Fragments arranged by size: 3364 1327 977 AgeI A'CCGG_T Cuts at: 4499 4499 Size: 5668 AhdI GACnn_n'nnGTC Cuts at: 2460 4397 2460 Size: 1937 3731 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5668 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5668 AlwNI CAG_nnn'CTG Cuts at: 2272 2770 3411 4983 2272 Size: 498 641 1572 2957 Fragments arranged by size: 2957 1572 641 498 ApaLI G'TGCA_C Cuts at: 2072 3725 4881 2072 Size: 1653 1156 2859 Fragments arranged by size: 2859 1653 1156 ApoI r'AATT_y Cuts at: 1493 2510 2977 3203 3355 5464 1493 Size: 1017 467 226 152 2109 1697 Fragments arranged by size: 2109 1697 1017 467 226 152 AseI AT'TA_AT Cuts at: 160 1032 4222 160 Size: 872 3190 1606 Fragments arranged by size: 3190 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5668 AvaI C'yCGr_G Cuts at: 1870 1943 2756 2915 2993 1870 Size: 73 813 159 78 4545 Fragments arranged by size: 4545 813 159 78 73 BamHI G'GATC_C Cuts at: 2998 3365 2998 Size: 367 5301 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2421 2948 2989 4444 618 Size: 325 217 627 634 527 41 1455 1842 Fragments arranged by size: 1842 1455 634 627 527 325 217 41 BanII G_rGCy'C Cuts at: 1391 2099 2630 2916 2987 1391 Size: 708 531 286 71 4072 Fragments arranged by size: 4072 708 531 286 71 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4697 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3859 3893 1903 Size: 34 1922 34 3678 Fragments arranged by size: 3678 1922 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3560 4770 3560 Size: 1210 4458 BclI T'GATC_A Cuts at: 1548 1548 Size: 5668 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2656 4279 136 Size: 107 122 71 631 605 984 1623 1525 Fragments arranged by size: 1623 1525 984 631 605 122 107 71 BglII A'GATC_T Cuts at: 5663 5663 Size: 5668 BlpI GC'TnA_GC Cuts at: 2093 2252 3085 2093 Size: 159 833 4676 Fragments arranged by size: 4676 833 159 Bme1580I G_kGCm'C Cuts at: 1790 2076 2951 3729 4885 1790 Size: 286 875 778 1156 2573 Fragments arranged by size: 2573 1156 875 778 286 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5668 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2573 3399 4357 Size: 416 53 83 399 24 1156 826 958 Cuts at: 4357 442 Size: 1753 Fragments arranged by size: 1753 1156 958 826 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5668 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4328 1239 Size: 3089 2579 Bpu10I CC'TnA_GC Cuts at: 2342 2342 Size: 5668 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3793 4658 4956 3793 Size: 865 298 4505 Fragments arranged by size: 4505 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4331 882 Size: 617 2832 2219 Fragments arranged by size: 2832 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3459 493 Size: 711 2255 2702 Fragments arranged by size: 2702 2255 711 BsaBI GATnn'nnATC Cuts at: 5662 5662 Size: 5668 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2936 3475 3857 4492 Size: 53 83 186 936 1403 539 382 635 Cuts at: 4492 275 Size: 1451 Fragments arranged by size: 1451 1403 936 635 539 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4101 4499 4773 4920 5331 4101 Size: 398 274 147 411 4438 Fragments arranged by size: 4438 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2448 2478 1178 Size: 30 165 30 1045 30 4368 Fragments arranged by size: 4368 1045 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2213 2638 2785 2866 2924 1186 Size: 1027 425 147 81 58 3930 Fragments arranged by size: 3930 1027 425 147 81 58 BseYI C'CCAG_C Cuts at: 2158 2265 2367 2631 4871 5367 2158 Size: 107 102 264 2240 496 2459 Fragments arranged by size: 2459 2240 496 264 107 102 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5095 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3118 3879 4907 1540 Size: 275 186 1117 761 1028 2301 Fragments arranged by size: 2301 1117 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2069 2076 2099 2630 2736 2892 3729 3814 4885 Size: 7 23 531 106 156 837 85 1071 Cuts at: 4885 2069 Size: 2852 Fragments arranged by size: 2852 1071 837 531 156 106 85 23 7 BsmI GAATG_Cn' Cuts at: 2175 3179 3272 2175 Size: 1004 93 4571 Fragments arranged by size: 4571 1004 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2927 2927 Size: 5668 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2069 2076 2099 2630 2736 2892 2916 Size: 399 279 7 23 531 106 156 24 Cuts at: 2916 2951 2987 3729 3814 4885 1391 Size: 35 36 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 531 399 279 156 106 85 36 35 24 23 7 BspEI T'CCGG_A Cuts at: 5331 5331 Size: 5668 BspHI T'CATG_A Cuts at: 3557 5287 3557 Size: 1730 3938 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2123 2755 3023 844 Size: 1279 632 268 3489 Fragments arranged by size: 3489 1279 632 268 BsrBI CCG'CTC Cuts at: 1394 1823 3555 1394 Size: 429 1732 3507 Fragments arranged by size: 3507 1732 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4163 4337 65 Size: 1183 2915 174 1396 Fragments arranged by size: 2915 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2412 4312 4499 1700 Size: 110 602 1900 187 2869 Fragments arranged by size: 2869 1900 602 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5668 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5668 BssSI C'ACGA_G Cuts at: 2297 3728 4740 2297 Size: 1431 1012 3225 Fragments arranged by size: 3225 1431 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5668 BstBI TT'CG_AA Cuts at: 2167 4472 2167 Size: 2305 3363 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5668 BstXI CCAn_nnnn'nTGG Cuts at: 2878 2959 2878 Size: 81 5587 BstYI r'GATC_y Cuts at: 1972 2998 3365 3750 3767 5219 5305 5317 5663 Size: 1026 367 385 17 1452 86 12 346 Cuts at: 5663 1972 Size: 1977 Fragments arranged by size: 1977 1452 1026 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2034 2943 513 Size: 552 969 909 3238 Fragments arranged by size: 3238 969 909 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4929 Fragments arranged by size: 4929 683 56 BtsI GCAGTG_nn' Cuts at: 2032 2642 3184 3978 3998 2032 Size: 610 542 794 20 3702 Fragments arranged by size: 3702 794 610 542 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5668 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5633 DraI TTT'AAA Cuts at: 2972 2972 Size: 5668 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2038 4675 809 Size: 507 722 2637 1802 Fragments arranged by size: 2637 1802 722 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2103 2355 3115 Size: 54 1425 50 275 18 273 252 760 Cuts at: 3115 4004 5633 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 760 275 273 252 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3115 1537 Size: 275 1303 4090 Fragments arranged by size: 4090 1303 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2231 2237 2303 2393 2491 3598 1353 Size: 878 6 66 90 98 1107 3423 Fragments arranged by size: 3423 1107 878 98 90 66 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4255 4629 4775 387 Size: 1064 138 2666 374 146 1280 Fragments arranged by size: 2666 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2097 2628 2097 Size: 531 5137 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2218 2515 2761 3731 4328 5115 5383 Size: 393 586 297 246 970 597 787 268 Cuts at: 5383 1239 Size: 1524 Fragments arranged by size: 1524 970 787 597 586 393 297 268 246 EcoO109I rG'GnC_Cy Cuts at: 2767 2874 2767 Size: 107 5561 EcoRI G'AATT_C Cuts at: 2977 3355 2977 Size: 378 5290 FspI TGC'GCA Cuts at: 4174 4174 Size: 5668 HaeII r_GCGC'y Cuts at: 1678 2374 2817 2903 4815 1678 Size: 696 443 86 1912 2531 Fragments arranged by size: 2531 1912 696 443 86 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2446 2478 4309 4341 4383 Size: 32 135 32 874 32 1831 32 42 Cuts at: 4383 4415 1373 Size: 32 2626 Fragments arranged by size: 2626 1831 874 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2481 3012 3258 669 Size: 988 281 543 531 246 3079 Fragments arranged by size: 3079 988 543 531 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5668 HpaI GTT'AAC Cuts at: 3258 3258 Size: 5668 KpnI G_GTAC'C Cuts at: 2993 2993 Size: 5668 MfeI C'AATT_G Cuts at: 3267 3267 Size: 5668 MluI A'CGCG_T Cuts at: 5544 5544 Size: 5668 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2917 4782 4966 1305 Size: 1612 1865 184 2007 Fragments arranged by size: 2007 1865 1612 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2105 10 Size: 54 1425 343 273 3573 Fragments arranged by size: 3573 1425 343 273 54 MslI CAynn'nnrTG Cuts at: 518 1166 3626 3985 4144 518 Size: 648 2460 359 159 2042 Fragments arranged by size: 2460 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3084 3761 4909 5154 1606 Size: 1478 677 1148 245 2120 Fragments arranged by size: 2120 1478 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5116 NdeI CA'TA_TG Cuts at: 387 387 Size: 5668 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5668 NotI GC'GGCC_GC Cuts at: 3115 3115 Size: 5668 NruI TCG'CGA Cuts at: 2907 2907 Size: 5668 NspI r_CATG'y Cuts at: 1132 1229 3026 4571 1132 Size: 97 1797 1545 2229 Fragments arranged by size: 2229 1797 1545 97 PasI CC'CwG_GG Cuts at: 1348 2878 1348 Size: 1530 4138 PciI A'CATG_T Cuts at: 4567 4567 Size: 5668 PflMI CCAn_nnn'nTGG Cuts at: 1108 2272 1108 Size: 1164 4504 PmeI GTTT'AAAC Cuts at: 2972 2972 Size: 5668 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3737 3769 5250 5282 3737 Size: 32 1481 32 4123 Fragments arranged by size: 4123 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2767 2874 2767 Size: 107 5561 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5668 PsiI TTA'TAA Cuts at: 3238 3238 Size: 5668 PstI C_TGCA'G Cuts at: 830 2498 3020 830 Size: 1668 522 3478 Fragments arranged by size: 3478 1668 522 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5668 SacI G_AGCT'C Cuts at: 2099 2630 2099 Size: 531 5137 SalI G'TCGA_C Cuts at: 1936 3010 1936 Size: 1074 4594 SbfI CC_TGCA'GG Cuts at: 3020 3020 Size: 5668 ScaI AGT'ACT Cuts at: 3916 5628 3916 Size: 1712 3956 SexAI A'CCwGG_T Cuts at: 2085 2870 2085 Size: 785 4883 SfcI C'TryA_G Cuts at: 826 1045 2494 3016 4151 4832 5023 826 Size: 219 1449 522 1135 681 191 1471 Fragments arranged by size: 1471 1449 1135 681 522 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5668 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5668 SmaI CCC'GGG Cuts at: 1872 2995 1872 Size: 1123 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3772 4673 4935 820 Size: 197 926 1829 901 262 1553 Fragments arranged by size: 1829 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3459 493 Size: 2966 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5668 SphI G_CATG'C Cuts at: 1132 3026 1132 Size: 1894 3774 SspI AAT'ATT Cuts at: 5 52 3592 5 Size: 47 3540 2081 Fragments arranged by size: 3540 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5668 StyI C'CwwG_G Cuts at: 513 1065 3107 513 Size: 552 2042 3074 Fragments arranged by size: 3074 2042 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2381 2425 2605 3695 3712 3865 4050 Size: 1255 801 44 180 1090 17 153 185 Cuts at: 4050 4495 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1090 801 445 185 180 153 44 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2319 3914 5626 Size: 274 80 33 51 1443 342 1595 1712 Cuts at: 5626 96 Size: 138 Fragments arranged by size: 1712 1595 1443 342 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2448 2480 5628 5660 2448 Size: 32 3148 32 2456 Fragments arranged by size: 3148 2456 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2291 2543 2590 1316 Size: 975 252 47 4394 Fragments arranged by size: 4394 975 252 47 XbaI T'CTAG_A Cuts at: 3004 3004 Size: 5668 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2254 2875 2254 Size: 621 5047 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5668 XmaI C'CCGG_G Cuts at: 1870 2993 1870 Size: 1123 4545 XmnI GAAnn'nnTTC Cuts at: 2183 3797 2183 Size: 1614 4054 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3476 276 Size: 53 83 186 936 1942 2468 Fragments arranged by size: 2468 1942 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI