Restriction map for
pFN21AB7390
(Linear) (Six-base) MAPSORT of: KIBB7390.seq Check: 9122 from: 1 to: 963
KIBB7390.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 118 963
Size: 118 845
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 213 510 756 963
Size: 213 297 246 207
Fragments arranged by size:
297 246 213 207
AfeI AGC'GCT
Cuts at: 0 367 963
Size: 367 596
AhdI GACnn_n'nnGTC
Cuts at: 0 455 963
Size: 455 508
AlwNI CAG_nnn'CTG
Cuts at: 0 267 765 963
Size: 267 498 198
Fragments arranged by size:
498 267 198
ApaLI G'TGCA_C
Cuts at: 0 67 963
Size: 67 896
ApoI r'AATT_y
Cuts at: 0 505 963
Size: 505 458
AvaI C'yCGr_G
Cuts at: 0 751 910 963
Size: 751 159 53
Fragments arranged by size:
751 159 53
BanI G'GyrC_C
Cuts at: 0 416 943 963
Size: 416 527 20
Fragments arranged by size:
527 416 20
BanII G_rGCy'C
Cuts at: 0 94 625 911 963
Size: 94 531 286 52
Fragments arranged by size:
531 286 94 52
BglI GCCn_nnn'nGGC
Cuts at: 0 651 963
Size: 651 312
BlpI GC'TnA_GC
Cuts at: 0 88 247 963
Size: 88 159 716
Fragments arranged by size:
716 159 88
Bme1580I G_kGCm'C
Cuts at: 0 71 946 963
Size: 71 875 17
Fragments arranged by size:
875 71 17
BmrI ACTGGGnnnn_n'
Cuts at: 0 568 963
Size: 568 395
Bpu10I CC'TnA_GC
Cuts at: 0 337 963
Size: 337 626
BsaHI Gr'CG_yC
Cuts at: 0 931 963
Size: 931 32
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 443 473 963
Size: 443 30 490
Fragments arranged by size:
490 443 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 208 633 780 861 919 963
Size: 208 425 147 81 58 44
Fragments arranged by size:
425 208 147 81 58 44
BseYI C'CCAG_C
Cuts at: 0 153 260 362 626 963
Size: 153 107 102 264 337
Fragments arranged by size:
337 264 153 107 102
BsiHKAI G_wGCw'C
Cuts at: 0 64 71 94 625 731 887 963
Size: 64 7 23 531 106 156 76
Fragments arranged by size:
531 156 106 76 64 23 7
BsmI GAATG_Cn'
Cuts at: 0 170 963
Size: 170 793
BsmBI CGTCTCn'nnnn_
Cuts at: 0 922 963
Size: 922 41
Bsp1286I G_dGCh'C
Cuts at: 0 64 71 94 625 731 887 911 946
Size: 64 7 23 531 106 156 24 35
Cuts at: 946 963
Size: 17
Fragments arranged by size:
531 156 106 64 35 24 23 17
7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 118 750 963
Size: 118 632 213
Fragments arranged by size:
632 213 118
BsrFI r'CCGG_y
Cuts at: 0 407 963
Size: 407 556
BssSI C'ACGA_G
Cuts at: 0 292 963
Size: 292 671
BstBI TT'CG_AA
Cuts at: 0 162 963
Size: 162 801
BstXI CCAn_nnnn'nTGG
Cuts at: 0 873 954 963
Size: 873 81 9
Fragments arranged by size:
873 81 9
BtgI C'CryG_G
Cuts at: 0 29 938 963
Size: 29 909 25
Fragments arranged by size:
909 29 25
BtsI GCAGTG_nn'
Cuts at: 0 27 637 963
Size: 27 610 326
Fragments arranged by size:
610 326 27
DrdI GACnn_nn'nnGTC
Cuts at: 0 33 963
Size: 33 930
EaeI y'GGCC_r
Cuts at: 0 98 350 963
Size: 98 252 613
Fragments arranged by size:
613 252 98
EarI CTCTTCn'nnn_
Cuts at: 0 226 232 298 388 486 963
Size: 226 6 66 90 98 477
Fragments arranged by size:
477 226 98 90 66 6
EcoICRI GAG'CTC
Cuts at: 0 92 623 963
Size: 92 531 340
Fragments arranged by size:
531 340 92
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 213 510 756 963
Size: 213 297 246 207
Fragments arranged by size:
297 246 213 207
EcoO109I rG'GnC_Cy
Cuts at: 0 762 869 963
Size: 762 107 94
Fragments arranged by size:
762 107 94
HaeII r_GCGC'y
Cuts at: 0 369 812 898 963
Size: 369 443 86 65
Fragments arranged by size:
443 369 86 65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 441 473 963
Size: 441 32 490
Fragments arranged by size:
490 441 32
HincII GTy'rAC
Cuts at: 0 476 963
Size: 476 487
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 912 963
Size: 912 51
MscI TGG'CCA
Cuts at: 0 100 963
Size: 100 863
NruI TCG'CGA
Cuts at: 0 902 963
Size: 902 61
PasI CC'CwG_GG
Cuts at: 0 873 963
Size: 873 90
PflMI CCAn_nnn'nTGG
Cuts at: 0 267 963
Size: 267 696
PpuMI rG'GwC_Cy
Cuts at: 0 762 869 963
Size: 762 107 94
Fragments arranged by size:
762 107 94
PstI C_TGCA'G
Cuts at: 0 493 963
Size: 493 470
SacI G_AGCT'C
Cuts at: 0 94 625 963
Size: 94 531 338
Fragments arranged by size:
531 338 94
SexAI A'CCwGG_T
Cuts at: 0 80 865 963
Size: 80 785 98
Fragments arranged by size:
785 98 80
SfcI C'TryA_G
Cuts at: 0 489 963
Size: 489 474
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 376 420 600 963
Size: 376 44 180 363
Fragments arranged by size:
376 363 180 44
TatI w'GTAC_w
Cuts at: 0 314 963
Size: 314 649
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 443 475 963
Size: 443 32 488
Fragments arranged by size:
488 443 32
Tth111I GACn'n_nGTC
Cuts at: 0 286 538 585 963
Size: 286 252 47 378
Fragments arranged by size:
378 286 252 47
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 249 870 963
Size: 249 621 93
Fragments arranged by size:
621 249 93
XmnI GAAnn'nnTTC
Cuts at: 0 178 963
Size: 178 785
Enzymes that do cut:
AarI AcuI AfeI AhdI AlwNI ApaLI ApoI AvaI
BanI BanII BglI BlpI Bme1580I BmrI Bpu10I BsaHI
BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrFI BssSI BstBI BstXI BtgI BtsI DrdI EaeI
EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII MmeI
MscI NruI PasI PflMI PpuMI PstI SacI SexAI
SfcI TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AleI
AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BmgBI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaWI BsgI BsiEI BsiWI BspEI BspHI BsrBI
BsrDI BsrGI BssHII BstAPI BstEII BstYI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII EagI EciI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KasI KpnI MfeI MluI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PacI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI XbaI XhoI XmaI ZraI