Restriction map for
pFN21AB7382
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AflII AflIII AgeI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspHI AspI AvaI AvaII AviII
AvrII BalI BamHI BanII BbrPI
BbvCI BcgI BciVI BfrI BglI
BlnI BlpI BmyI BpmI Bpu1102I
BsaBI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BsrI BssHII
BssSI BstAPI BstUI BstXI Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoRI EcoRV EcoT14I EcoT22I FauI
FseI FspI HgaI HincII HindII
HindIII HpaI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MseI MunI MvnI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfcI SfiI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tru9I Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 70 enzymes) #
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AcsI 31 GAATTT [GA]^AATT[CT]
AcyI 695 GGCGCC G[GA]^CG[CT]C
AfaI 455 GTAC GT^AC
AhdI 486 GACTATTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 31 GAATTT [GA]^AATT[CT]
Asp700I 846 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 486 GACTATTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 695 GGCGCC G^G[CT][GA]CC
BbeI 695 GGCGCC GGCGC^C
BbvI 897 GCAGC GCAGC(8/12)
BclI 344 TGATCA T^GATCA
BcnI 60 CCGGG CC^[GC]GG
BfaI 469 CTAG C^TAG
BglII 332 AGATCT A^GATCT
BsaAI 367 TACGTG [CT]AC^GT[GA]
BsaHI 695 GGCGCC G[GA]^CG[CT]C
BsaJI 638 CCGTGG C^C[ACGT][ACGT]GG
BseRI 874 GAGGAG GAGGAG(10/8)
BsmFI 45 GGGAC GGGAC(10/14)
BsmI 88 GCATTC GAATGC(1/-1)
BspMI 715 ACCTGC ACCTGC(4/8)
BsrDI 138 CATTGC GCAATG(2/0)
Bst1107I 422 GTATAC GTA^TAC
BstBI 674 TTCGAA TT^CGAA
BstF5I 660 CATCC GGATG(2/0)
BstNI 663 CCTGG CC^[AT]GG
BstYI 332 AGATCT [GA]^GATC[CT]
BstZ17I 422 GTATAC GTA^TAC
CfoI 696 GCGC GCG^C
DsaI 638 CCGTGG C^C[GA][CT]GG
Eam1105I 486 GACTATTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRII 663 CCTGG ^CC[AT]GG
FbaI 344 TGATCA T^GATCA
Fnu4HI 897 GCAGC GC^[ACGT]GC
FokI 660 CATCC GGATG(9/13)
HaeII 695 GGCGCC [GA]GCGC^[CT]
HapII 60 CCGG C^CGG
HhaI 696 GCGC GCG^C
Hin1I 695 GGCGCC G[GA]^CG[CT]C
HinP1I 696 GCGC G^CGC
HpaII 60 CCGG C^CGG
ItaI 897 GCAGC GC^[ACGT]GC
KasI 695 GGCGCC G^GCGCC
MaeI 469 CTAG C^TAG
MaeII 368 ACGT A^CGT
MflI 332 AGATCT [GA]^GATC[CT]
MslI 99 CATTTATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 686 CAGCTG C[AC]G^C[GT]G
MspI 60 CCGG C^CGG
MvaI 663 CCTGG CC^[AT]GG
NarI 695 GGCGCC GG^CGCC
NciI 60 CCGGG CC^[GC]GG
NspV 674 TTCGAA TT^CGAA
PflMI 726 CCACTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 686 CAGCTG CAG^CTG
RsaI 455 GTAC GT^AC
SapI 300 GCTCTTC GCTCTTC(1/4)
SfaNI 290 GCATC GCATC(5/9)
SfoI 695 GGCGCC GGC^GCC
SfuI 674 TTCGAA TT^CGAA
SmlI 864 CTTGAG C^T[CT][GA]AG
TaqI 675 TCGA T^CGA
TfiI 846 GAATC G^A[AT]TC
TseI 897 GCAGC G^C[AT]GC
Tsp509I 32 AATT ^AATT
TthHB8I 675 TCGA T^CGA
Van91I 726 CCACTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 797 CCATTGACAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 332 AGATCT [GA]^GATC[CT]
XmnI 846 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
BbsI 37 GTCTTC GAAGAC(2/6)
817 GTCTTC
BpuAI 37 GTCTTC GAAGAC(2/6)
817 GTCTTC
BsgI 378 CTGCAC GTGCAG(16/14)
564 CTGCAC
BsmAI 909 GAGAC GTCTC(1/5)
161 GTCTC
BssKI 60 CCGGG ^CC[ACGT]GG
663 CCTGG
BstEII 642 GGTCACC G^GT[ACGT]ACC
831 GGTCACC
BstPI 642 GGTCACC G^GT[ACGT]ACC
831 GGTCACC
Cac8I 558 GCTGGC GC[ACGT]^[ACGT]GC
562 GCCTGC
EaeI 635 TGGCCG [CT]^GGCC[GA]
778 CGGCCA
EcoO65I 642 GGTCACC G^GT[ACGT]ACC
831 GGTCACC
HphI 644 TCACC GGTGA(8/7)
833 TCACC
PleI 47 GACTC GAGTC(4/5)
416 GAGTC
ScrFI 60 CCGGG CC^[ACGT]GG
663 CCTGG
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# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AccI AluI BsiYI BslI Cfr13I
DdeI DpnII EarI HaeIII HinfI
Ksp632I MaeIII MboI MboII MnlI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I Tsp45I TspRI