Restriction map for
pFN21AB7368
(Circular) (Six-base) MAPSORT of: pFN21AB7368.seq Check: 198 from: 1 to: 5653
pFN21AB7368 5653 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2243 2243
Size: 5653
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3463 278
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2996 1937
Size: 1059 4594
Acc65I G'GTAC_C
Cuts at: 2974 2974
Size: 5653
AclI AA'CG_TT
Cuts at: 1563 3780 4153 1563
Size: 2217 373 3063
Fragments arranged by size:
3063 2217 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2601 3716 5100 5368 1632
Size: 969 1115 1384 268 1917
Fragments arranged by size:
1917 1384 1115 969 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5456
AflIII A'CryG_T
Cuts at: 1203 4552 5529 1203
Size: 3349 977 1327
Fragments arranged by size:
3349 1327 977
AgeI A'CCGG_T
Cuts at: 4484 4484
Size: 5653
AhdI GACnn_n'nnGTC
Cuts at: 4382 4382
Size: 5653
AleI CACnn'nnGTG
Cuts at: 1166 2748 1166
Size: 1582 4071
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5653
AlwNI CAG_nnn'CTG
Cuts at: 3396 4968 3396
Size: 1572 4081
ApaLI G'TGCA_C
Cuts at: 3710 4866 3710
Size: 1156 4497
ApoI r'AATT_y
Cuts at: 1493 2134 2673 2907 2962 3188 3340 5449 1493
Size: 641 539 234 55 226 152 2109 1697
Fragments arranged by size:
2109 1697 641 539 234 226 152 55
AseI AT'TA_AT
Cuts at: 160 1032 4207 160
Size: 872 3175 1606
Fragments arranged by size:
3175 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
AvaI C'yCGr_G
Cuts at: 1870 1943 2978 1870
Size: 73 1035 4545
Fragments arranged by size:
4545 1035 73
AvrII C'CTAG_G
Cuts at: 2632 2632
Size: 5653
BamHI G'GATC_C
Cuts at: 2983 3350 2983
Size: 367 5286
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2974 4429 618
Size: 325 217 627 1187 1455 1842
Fragments arranged by size:
1842 1455 1187 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2496 2972 1391
Size: 1105 476 4072
Fragments arranged by size:
4072 1105 476
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2020 2746 2903 928
Size: 971 121 726 157 3678
Fragments arranged by size:
3678 971 726 157 121
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2325 2359 3844 3878 1903
Size: 34 50 34 304 34 1485 34 3678
Fragments arranged by size:
3678 1485 304 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2360 2402 3545 4755 2360
Size: 42 1143 1210 3258
Fragments arranged by size:
3258 1210 1143 42
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5653
BfrBI ATG'CAT
Cuts at: 2736 2736
Size: 5653
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2207 4264 136
Size: 107 122 71 631 605 535 2057 1525
Fragments arranged by size:
2057 1525 631 605 535 122 107 71
BglII A'GATC_T
Cuts at: 5648 5648
Size: 5653
BlpI GC'TnA_GC
Cuts at: 3070 3070
Size: 5653
Bme1580I G_kGCm'C
Cuts at: 1790 3714 4870 1790
Size: 1924 1156 2573
Fragments arranged by size:
2573 1924 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5653
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3384 4342 442
Size: 416 53 83 399 24 1967 958 1753
Fragments arranged by size:
1967 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2090 4313 1239
Size: 851 2223 2579
Fragments arranged by size:
2579 2223 851
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2419 3778 4643 4941 2419
Size: 1359 865 298 3131
Fragments arranged by size:
3131 1359 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4316 882
Size: 617 2817 2219
Fragments arranged by size:
2817 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3444 493
Size: 711 2240 2702
Fragments arranged by size:
2702 2240 711
BsaBI GATnn'nnATC
Cuts at: 5647 5647
Size: 5653
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3460 3842 4477 275
Size: 53 83 186 936 1927 382 635 1451
Fragments arranged by size:
1927 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4086 4484 4758 4905 5316 4086
Size: 398 274 147 411 4423
Fragments arranged by size:
4423 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5428
Fragments arranged by size:
5428 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5653
BseYI C'CCAG_C
Cuts at: 2421 4856 5352 2421
Size: 2435 496 2722
Fragments arranged by size:
2722 2435 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2099 1163
Size: 573 363 4717
Fragments arranged by size:
4717 573 363
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3103 3864 4892 1540
Size: 275 186 1102 761 1028 2301
Fragments arranged by size:
2301 1102 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2556 3714 3799 4870 2556
Size: 1158 85 1071 3339
Fragments arranged by size:
3339 1158 1071 85
BsmI GAATG_Cn'
Cuts at: 2238 3164 3257 2238
Size: 926 93 4634
Fragments arranged by size:
4634 926 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2496 2556 2972 3714 3799 4870 1391
Size: 399 706 60 416 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 706 416 399 85 60
BspEI T'CCGG_A
Cuts at: 5316 5316
Size: 5653
BspHI T'CATG_A
Cuts at: 3542 5272 3542
Size: 1730 3923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2243 3008 844
Size: 1399 765 3489
Fragments arranged by size:
3489 1399 765
BsrBI CCG'CTC
Cuts at: 1394 1823 3540 1394
Size: 429 1717 3507
Fragments arranged by size:
3507 1717 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2220 2367 2736 4148 4322 65
Size: 1183 972 147 369 1412 174 1396
Fragments arranged by size:
1412 1396 1183 972 369 174 147
BsrFI r'CCGG_y
Cuts at: 1700 1810 4297 4484 1700
Size: 110 2487 187 2869
Fragments arranged by size:
2869 2487 187 110
BsrGI T'GTAC_A
Cuts at: 96 2187 96
Size: 2091 3562
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5653
BssSI C'ACGA_G
Cuts at: 3713 4725 3713
Size: 1012 4641
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5653
BstBI TT'CG_AA
Cuts at: 4457 4457
Size: 5653
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5653
BstYI r'GATC_y
Cuts at: 1972 2983 3350 3735 3752 5204 5290 5302 5648
Size: 1011 367 385 17 1452 86 12 346
Cuts at: 5648 1972
Size: 1977
Fragments arranged by size:
1977 1452 1011 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2488 2488
Size: 5653
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5101
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4914
Fragments arranged by size:
4914 683 56
BtsI GCAGTG_nn'
Cuts at: 2087 3169 3963 3983 2087
Size: 1082 794 20 3757
Fragments arranged by size:
3757 1082 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5653
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2203 2238 529
Size: 35 1639 35 3944
Fragments arranged by size:
3944 1639 35 35
DraI TTT'AAA
Cuts at: 2957 2957
Size: 5653
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4660 809
Size: 507 3344 1802
Fragments arranged by size:
3344 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2199 2382 2559
Size: 54 1425 50 275 18 369 183 177
Cuts at: 2559 3100 3989 5618 8
Size: 541 889 1629 43
Fragments arranged by size:
1629 1425 889 541 369 275 183 177
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3100 1537
Size: 275 1288 4090
Fragments arranged by size:
4090 1288 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3583 1353
Size: 2230 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4240 4614 4760 387
Size: 1064 138 2651 374 146 1280
Fragments arranged by size:
2651 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2601 3716 4313 5100 5368 1239
Size: 393 458 511 1115 597 787 268 1524
Fragments arranged by size:
1524 1115 787 597 511 458 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2487 2487
Size: 5653
EcoRI G'AATT_C
Cuts at: 2962 3340 2962
Size: 378 5275
EcoRV GAT'ATC
Cuts at: 2587 2587
Size: 5653
FspI TGC'GCA
Cuts at: 4159 4159
Size: 5653
HaeII r_GCGC'y
Cuts at: 1678 4800 1678
Size: 3122 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2050 2082 4294 4326 4368
Size: 32 135 32 478 32 2212 32 42
Cuts at: 4368 4400 1373
Size: 32 2626
Fragments arranged by size:
2626 2212 478 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2596 2997 3243 669
Size: 988 281 658 401 246 3079
Fragments arranged by size:
3079 988 658 401 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5653
HpaI GTT'AAC
Cuts at: 3243 3243
Size: 5653
KpnI G_GTAC'C
Cuts at: 2978 2978
Size: 5653
MfeI C'AATT_G
Cuts at: 2337 3252 2337
Size: 915 4738
MluI A'CGCG_T
Cuts at: 5529 5529
Size: 5653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2066 4767 4951 1305
Size: 761 2701 184 2007
Fragments arranged by size:
2701 2007 761 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2201 2384 2561 10
Size: 54 1425 343 369 183 177 3102
Fragments arranged by size:
3102 1425 369 343 183 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2748 3611 3970 4129 518
Size: 648 1582 863 359 159 2042
Fragments arranged by size:
2042 1582 863 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2695 3069 3746 4894 5139 1606
Size: 1089 374 677 1148 245 2120
Fragments arranged by size:
2120 1148 1089 677 374 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5101
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5653
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5653
NotI GC'GGCC_GC
Cuts at: 3100 3100
Size: 5653
NsiI A_TGCA'T
Cuts at: 2738 2738
Size: 5653
NspI r_CATG'y
Cuts at: 1132 1229 2408 2736 3011 4556 1132
Size: 97 1179 328 275 1545 2229
Fragments arranged by size:
2229 1545 1179 328 275 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5653
PciI A'CATG_T
Cuts at: 4552 4552
Size: 5653
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5653
PmeI GTTT'AAAC
Cuts at: 2957 2957
Size: 5653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3722 3754 5235 5267 3722
Size: 32 1481 32 4108
Fragments arranged by size:
4108 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5653
PsiI TTA'TAA
Cuts at: 2153 2477 2654 3223 2153
Size: 324 177 569 4583
Fragments arranged by size:
4583 569 324 177
PstI C_TGCA'G
Cuts at: 830 3005 830
Size: 2175 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5653
PvuII CAG'CTG
Cuts at: 2695 2695
Size: 5653
SalI G'TCGA_C
Cuts at: 1936 2995 1936
Size: 1059 4594
SbfI CC_TGCA'GG
Cuts at: 3005 3005
Size: 5653
ScaI AGT'ACT
Cuts at: 3901 5613 3901
Size: 1712 3941
SfcI C'TryA_G
Cuts at: 826 1045 2526 2759 3001 4136 4817 5008 826
Size: 219 1481 233 242 1135 681 191 1471
Fragments arranged by size:
1481 1471 1135 681 242 233 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5653
SmaI CCC'GGG
Cuts at: 1872 2980 1872
Size: 1108 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2398 3757 4658 4920 820
Size: 197 926 455 1359 901 262 1553
Fragments arranged by size:
1553 1359 926 901 455 262 197
SnaBI TAC'GTA
Cuts at: 493 3444 493
Size: 2951 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5653
SphI G_CATG'C
Cuts at: 1132 3011 1132
Size: 1879 3774
SspI AAT'ATT
Cuts at: 5 52 2649 3577 5
Size: 47 2597 928 2081
Fragments arranged by size:
2597 2081 928 47
StuI AGG'CCT
Cuts at: 1517 2723 1517
Size: 1206 4447
StyI C'CwwG_G
Cuts at: 513 1065 2632 3092 513
Size: 552 1567 460 3074
Fragments arranged by size:
3074 1567 552 460
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3680 3697 3850 4035 4480 325
Size: 1255 2100 17 153 185 445 1498
Fragments arranged by size:
2100 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2187 3899 5611
Size: 274 80 33 51 1443 210 1712 1712
Cuts at: 5611 96
Size: 138
Fragments arranged by size:
1712 1712 1443 274 210 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2436 2468 5613 5645 2436
Size: 32 3145 32 2444
Fragments arranged by size:
3145 2444 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5653
XbaI T'CTAG_A
Cuts at: 2989 2989
Size: 5653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2569 2569
Size: 5653
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5653
XmaI C'CCGG_G
Cuts at: 1870 2978 1870
Size: 1108 4545
XmnI GAAnn'nnTTC
Cuts at: 3782 3782
Size: 5653
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3461 276
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoO109I
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI