(Circular) (Six-base) MAPSORT of: pFN21AB7362.seq Check: 5533 from: 1 to: 5461 pFN21AB7362 5461 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3271 278 Size: 53 83 186 936 1735 2468 Fragments arranged by size: 2468 1735 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2804 1937 Size: 867 4594 Acc65I G'GTAC_C Cuts at: 2782 2782 Size: 5461 AclI AA'CG_TT Cuts at: 1563 3588 3961 1563 Size: 2025 373 3063 Fragments arranged by size: 3063 2025 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3524 4908 5176 1632 Size: 1892 1384 268 1917 Fragments arranged by size: 1917 1892 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2548 820 Size: 197 1531 3733 Fragments arranged by size: 3733 1531 197 AflIII A'CryG_T Cuts at: 1203 4360 5337 1203 Size: 3157 977 1327 Fragments arranged by size: 3157 1327 977 AgeI A'CCGG_T Cuts at: 4292 4292 Size: 5461 AhdI GACnn_n'nnGTC Cuts at: 4190 4190 Size: 5461 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5461 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5461 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2075 2107 2075 Size: 32 5429 AlwNI CAG_nnn'CTG Cuts at: 3204 4776 3204 Size: 1572 3889 ApaI G_GGCC'C Cuts at: 2112 2143 2112 Size: 31 5430 ApaLI G'TGCA_C Cuts at: 3518 4674 3518 Size: 1156 4305 ApoI r'AATT_y Cuts at: 1493 2770 2996 3148 5257 1493 Size: 1277 226 152 2109 1697 Fragments arranged by size: 2109 1697 1277 226 152 AseI AT'TA_AT Cuts at: 160 1032 4015 160 Size: 872 2983 1606 Fragments arranged by size: 2983 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5461 AvaI C'yCGr_G Cuts at: 1870 1943 2063 2096 2786 1870 Size: 73 120 33 690 4545 Fragments arranged by size: 4545 690 120 73 33 BamHI G'GATC_C Cuts at: 2791 3158 2791 Size: 367 5094 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2102 2504 2782 4237 618 Size: 325 217 627 315 402 278 1455 1842 Fragments arranged by size: 1842 1455 627 402 325 315 278 217 BanII G_rGCy'C Cuts at: 1391 2112 2143 2181 2780 1391 Size: 721 31 38 599 4072 Fragments arranged by size: 4072 721 599 38 31 BbeI G_GCGC'C Cuts at: 2106 2106 Size: 5461 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2646 2738 928 Size: 971 747 92 3651 Fragments arranged by size: 3651 971 747 92 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3652 3686 1903 Size: 34 1715 34 3678 Fragments arranged by size: 3678 1715 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3353 4563 3353 Size: 1210 4251 BclI T'GATC_A Cuts at: 1548 1548 Size: 5461 BfrBI ATG'CAT Cuts at: 2748 2748 Size: 5461 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4072 136 Size: 107 122 71 631 605 2400 1525 Fragments arranged by size: 2400 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2242 5456 2242 Size: 3214 2247 BlpI GC'TnA_GC Cuts at: 2878 2878 Size: 5461 Bme1580I G_kGCm'C Cuts at: 1790 2112 2143 2540 3522 4678 1790 Size: 322 31 397 982 1156 2573 Fragments arranged by size: 2573 1156 982 397 322 31 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5461 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3192 4150 442 Size: 416 53 83 399 24 1775 958 1753 Fragments arranged by size: 1775 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5461 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2689 4121 1239 Size: 1450 1432 2579 Fragments arranged by size: 2579 1450 1432 Bpu10I CC'TnA_GC Cuts at: 2272 2272 Size: 5461 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3586 4451 4749 3586 Size: 865 298 4298 Fragments arranged by size: 4298 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4124 882 Size: 617 2625 2219 Fragments arranged by size: 2625 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2116 3252 493 Size: 711 912 1136 2702 Fragments arranged by size: 2702 1136 912 711 BsaBI GATnn'nnATC Cuts at: 5455 5455 Size: 5461 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2103 3268 3650 4285 Size: 53 83 186 936 570 1165 382 635 Cuts at: 4285 275 Size: 1451 Fragments arranged by size: 1451 1165 936 635 570 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3894 4292 4566 4713 5124 3894 Size: 398 274 147 411 4231 Fragments arranged by size: 4231 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5236 Fragments arranged by size: 5236 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5461 BseYI C'CCAG_C Cuts at: 2584 4664 5160 2584 Size: 2080 496 2885 Fragments arranged by size: 2885 2080 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4888 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2911 3672 4700 1540 Size: 275 186 910 761 1028 2301 Fragments arranged by size: 2301 1028 910 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2340 2441 2607 3522 3607 4678 2340 Size: 101 166 915 85 1071 3123 Fragments arranged by size: 3123 1071 915 166 101 85 BsmI GAATG_Cn' Cuts at: 2972 3065 2972 Size: 93 5368 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2112 2143 2181 2340 2441 2540 2607 Size: 399 322 31 38 159 101 99 67 Cuts at: 2607 2780 3522 3607 4678 1391 Size: 173 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 322 173 159 101 99 85 67 38 31 BspEI T'CCGG_A Cuts at: 5124 5124 Size: 5461 BspHI T'CATG_A Cuts at: 3350 5080 3350 Size: 1730 3731 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2816 844 Size: 1972 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2365 3348 1394 Size: 429 542 983 3507 Fragments arranged by size: 3507 983 542 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3956 4130 65 Size: 1183 2708 174 1396 Fragments arranged by size: 2708 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2074 4105 4292 1700 Size: 110 264 2031 187 2869 Fragments arranged by size: 2869 2031 264 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5461 BssHII G'CGCG_C Cuts at: 1925 2011 2013 1925 Size: 86 2 5373 Fragments arranged by size: 5373 86 2 BssSI C'ACGA_G Cuts at: 3521 4533 3521 Size: 1012 4449 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5461 BstBI TT'CG_AA Cuts at: 4265 4265 Size: 5461 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5461 BstXI CCAn_nnnn'nTGG Cuts at: 2584 2584 Size: 5461 BstYI r'GATC_y Cuts at: 1972 2242 2791 3158 3543 3560 5012 5098 5110 Size: 270 549 367 385 17 1452 86 12 Cuts at: 5110 5456 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 549 385 367 346 270 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2056 2304 2347 2446 513 Size: 552 991 248 43 99 3528 Fragments arranged by size: 3528 991 552 248 99 43 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4722 Fragments arranged by size: 4722 683 56 BtsI GCAGTG_nn' Cuts at: 2315 2977 3771 3791 2315 Size: 662 794 20 3985 Fragments arranged by size: 3985 794 662 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5461 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2561 2596 529 Size: 35 1997 35 3394 Fragments arranged by size: 3394 1997 35 35 DraI TTT'AAA Cuts at: 2765 2765 Size: 5461 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4468 809 Size: 507 3152 1802 Fragments arranged by size: 3152 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2908 3797 5426 Size: 54 1425 50 275 18 1078 889 1629 Cuts at: 5426 8 Size: 43 Fragments arranged by size: 1629 1425 1078 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2908 1537 Size: 275 1096 4090 Fragments arranged by size: 4090 1096 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2376 3391 1353 Size: 1023 1015 3423 Fragments arranged by size: 3423 1023 1015 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2474 4048 4422 4568 387 Size: 1064 138 885 1574 374 146 1280 Fragments arranged by size: 1574 1280 1064 885 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2689 3524 4121 4908 5176 1239 Size: 393 1057 835 597 787 268 1524 Fragments arranged by size: 1524 1057 835 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2126 2126 Size: 5461 EcoO109I rG'GnC_Cy Cuts at: 2109 2109 Size: 5461 EcoRI G'AATT_C Cuts at: 2770 3148 2770 Size: 378 5083 FspI TGC'GCA Cuts at: 3967 3967 Size: 5461 HaeII r_GCGC'y Cuts at: 1678 2056 2106 2468 4608 1678 Size: 378 50 362 2140 2531 Fragments arranged by size: 2531 2140 378 362 50 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4102 4134 4176 4208 1373 Size: 32 135 32 2530 32 42 32 2626 Fragments arranged by size: 2626 2530 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2805 3051 669 Size: 988 281 867 246 3079 Fragments arranged by size: 3079 988 867 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5461 HpaI GTT'AAC Cuts at: 3051 3051 Size: 5461 KasI G'GCGC_C Cuts at: 2102 2102 Size: 5461 KpnI G_GTAC'C Cuts at: 2786 2786 Size: 5461 MfeI C'AATT_G Cuts at: 3060 3060 Size: 5461 MluI A'CGCG_T Cuts at: 5337 5337 Size: 5461 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2313 2358 4575 4759 1305 Size: 1008 45 2217 184 2007 Fragments arranged by size: 2217 2007 1008 184 45 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3639 Fragments arranged by size: 3639 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2743 2753 3419 3778 3937 518 Size: 648 1577 10 666 359 159 2042 Fragments arranged by size: 2042 1577 666 648 359 159 10 MspA1I CmG'CkG Cuts at: 1606 2058 2497 2877 3554 4702 4947 1606 Size: 452 439 380 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 452 439 380 245 NaeI GCC'GGC Cuts at: 2076 2076 Size: 5461 NarI GG'CG_CC Cuts at: 2103 2103 Size: 5461 NcoI C'CATG_G Cuts at: 513 1065 2304 2446 513 Size: 552 1239 142 3528 Fragments arranged by size: 3528 1239 552 142 NdeI CA'TA_TG Cuts at: 387 387 Size: 5461 NgoMIV G'CCGG_C Cuts at: 2074 2074 Size: 5461 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5461 NotI GC'GGCC_GC Cuts at: 2908 2908 Size: 5461 NsiI A_TGCA'T Cuts at: 2750 2750 Size: 5461 NspI r_CATG'y Cuts at: 1132 1229 2819 4364 1132 Size: 97 1590 1545 2229 Fragments arranged by size: 2229 1590 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5461 PciI A'CATG_T Cuts at: 4360 4360 Size: 5461 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5461 PmeI GTTT'AAAC Cuts at: 2765 2765 Size: 5461 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3530 3562 5043 5075 3530 Size: 32 1481 32 3916 Fragments arranged by size: 3916 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5461 PsiI TTA'TAA Cuts at: 3031 3031 Size: 5461 PspOMI G'GGCC_C Cuts at: 2108 2139 2108 Size: 31 5430 PstI C_TGCA'G Cuts at: 830 2322 2813 830 Size: 1492 491 3478 Fragments arranged by size: 3478 1492 491 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5461 SacII CC_GC'GG Cuts at: 2059 2059 Size: 5461 SalI G'TCGA_C Cuts at: 1936 2803 1936 Size: 867 4594 SbfI CC_TGCA'GG Cuts at: 2813 2813 Size: 5461 ScaI AGT'ACT Cuts at: 3709 5421 3709 Size: 1712 3749 SfcI C'TryA_G Cuts at: 826 1045 2318 2809 3944 4625 4816 826 Size: 219 1273 491 1135 681 191 1471 Fragments arranged by size: 1471 1273 1135 681 491 219 191 SfoI GGC'GCC Cuts at: 2104 2104 Size: 5461 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5461 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5461 SmaI CCC'GGG Cuts at: 1872 2788 1872 Size: 916 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2548 3565 4466 4728 820 Size: 197 926 605 1017 901 262 1553 Fragments arranged by size: 1553 1017 926 901 605 262 197 SnaBI TAC'GTA Cuts at: 493 3252 493 Size: 2759 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5461 SphI G_CATG'C Cuts at: 1132 2819 1132 Size: 1687 3774 SspI AAT'ATT Cuts at: 5 52 3385 5 Size: 47 3333 2081 Fragments arranged by size: 3333 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5461 StyI C'CwwG_G Cuts at: 513 1065 2304 2446 2900 513 Size: 552 1239 142 454 3074 Fragments arranged by size: 3074 1239 552 454 142 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3488 3505 3658 3843 4288 325 Size: 1255 1908 17 153 185 445 1498 Fragments arranged by size: 1908 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3707 5419 96 Size: 274 80 33 51 1443 1730 1712 138 Fragments arranged by size: 1730 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5421 5453 5421 Size: 32 5429 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5461 XbaI T'CTAG_A Cuts at: 2596 2797 2596 Size: 201 5260 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5461 XmaI C'CCGG_G Cuts at: 1870 2786 1870 Size: 916 4545 XmnI GAAnn'nnTTC Cuts at: 3590 3590 Size: 5461 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3269 276 Size: 53 83 186 936 1735 2468 Fragments arranged by size: 2468 1735 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI