Restriction map for
pFN21AB7361
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbvCI BcgI BciVI BcnI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmFI BsoBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MaeI MamI MfeI MluI
MluNI MroI MscI MspI MunI
MvnI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AciI 357 CCGC CCGC(-3/-1)
AlwNI 314 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 598 ATTAAT AT^TAAT
AsnI 598 ATTAAT AT^TAAT
AspHI 408 GAGCAC G[AT]GC[AT]^C
BanI 478 GGCACC G^G[CT][GA]CC
BbsI 655 GTCTTC GAAGAC(2/6)
BclI 134 TGATCA T^GATCA
BpuAI 655 GTCTTC GAAGAC(2/6)
BsgI 382 CTGCAC GTGCAG(16/14)
BsiHKAI 408 GAGCAC G[AT]GC[AT]^C
BsmAI 786 GTCTC GTCTC(1/5)
BsmI 72 GCATTC GAATGC(1/-1)
BspHI 131 TCATGA T^CATGA
BsrDI 759 CATTGC GCAATG(2/0)
BssKI 291 CCAGG ^CC[ACGT]GG
BstNI 291 CCAGG CC^[AT]GG
BstXI 878 CCATAAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 561 CTCAG C^T[ACGT]AG
EarI 82 CTCTTC CTCTTC(1/4)
EcoRII 291 CCAGG ^CC[AT]GG
EcoT14I 276 CCTTGG C^C[AT][AT]GG
FbaI 134 TGATCA T^GATCA
Ksp632I 82 CTCTTC CTCTTC(1/4)
MaeII 653 ACGT A^CGT
MspA1I 473 CAGCTG C[AC]G^C[GT]G
MvaI 291 CCAGG CC^[AT]GG
PleI 784 GAGTC GAGTC(4/5)
PshBI 598 ATTAAT AT^TAAT
PstI 320 CTGCAG CTGCA^G
PvuII 473 CAGCTG CAG^CTG
RcaI 131 TCATGA T^CATGA
ScrFI 291 CCAGG CC^[ACGT]GG
StyI 276 CCTTGG C^C[AT][AT]GG
TaqI 76 TCGA T^CGA
TthHB8I 76 TCGA T^CGA
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# TWO CUT ENZYMES ( 18 enzymes) #
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AcsI 25 GAATTT [GA]^AATT[CT]
567 AAATTT
AlwI 675 GATCC GGATC(4/5)
201 GGATC
ApoI 25 GAATTT [GA]^AATT[CT]
567 AAATTT
BbvI 327 GCTGC GCAGC(8/12)
831 GCTGC
BmyI 104 GTGCCC G[AGT]GC[ACT]^C
408 GAGCAC
BsaJI 276 CCTTGG C^C[ACGT][ACGT]GG
290 CCCAGG
BseRI 331 CTCCTC GAGGAG(10/8)
334 CTCCTC
Bsp1286I 104 GTGCCC G[AGT]GC[ACT]^C
408 GAGCAC
BstYI 201 GGATCT [GA]^GATC[CT]
674 AGATCC
Cac8I 475 GCTGGC GC[ACGT]^[ACGT]GC
774 GCCAGC
Fnu4HI 327 GCTGC GC^[ACGT]GC
831 GCTGC
HaeIII 691 GGCC GG^CC
876 GGCC
ItaI 327 GCTGC GC^[ACGT]GC
831 GCTGC
MflI 201 GGATCT [GA]^GATC[CT]
674 AGATCC
SfcI 320 CTGCAG C^T[GA][CT]AG
662 CTACAG
TseI 327 GCTGC G^C[AT]GC
831 GCTGC
TspRI 429 CACTG CA[GC]TG[ACGT][ACGT]^
750 CACTG
XhoII 201 GGATCT [GA]^GATC[CT]
674 AGATCC
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AluI AvaII BsiYI BslI BsrI
BstF5I Cfr13I DpnII FokI HinfI
HphI MaeIII MboI MboII MnlI
MseI MslI NdeII NlaIII NlaIV
Sau3AI Sau96I SfaNI TfiI Tru9I
Tsp45I Tsp509I