(Circular) (Six-base) MAPSORT of: pFN21AB7358.seq Check: 2429 from: 1 to: 5962 pFN21AB7358 5962 bp With 182 enzymes: SgfI * March 28, 2012 14:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3772 278 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3305 1937 Size: 1368 4594 Acc65I G'GTAC_C Cuts at: 2082 3283 2082 Size: 1201 4761 AclI AA'CG_TT Cuts at: 1563 4089 4462 1563 Size: 2526 373 3063 Fragments arranged by size: 3063 2526 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2610 2852 2886 4025 5409 5677 1632 Size: 978 242 34 1139 1384 268 1917 Fragments arranged by size: 1917 1384 1139 978 268 242 34 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5765 AflIII A'CryG_T Cuts at: 1203 4861 5838 1203 Size: 3658 977 1327 Fragments arranged by size: 3658 1327 977 AgeI A'CCGG_T Cuts at: 4793 4793 Size: 5962 AhdI GACnn_n'nnGTC Cuts at: 4691 4691 Size: 5962 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5962 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5962 AlwNI CAG_nnn'CTG Cuts at: 3705 5277 3705 Size: 1572 4390 ApaLI G'TGCA_C Cuts at: 4019 5175 4019 Size: 1156 4806 ApoI r'AATT_y Cuts at: 1493 3271 3497 3649 5758 1493 Size: 1778 226 152 2109 1697 Fragments arranged by size: 2109 1778 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4516 160 Size: 872 3484 1606 Fragments arranged by size: 3484 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 AvaI C'yCGr_G Cuts at: 1870 1943 3287 1870 Size: 73 1344 4545 Fragments arranged by size: 4545 1344 73 BamHI G'GATC_C Cuts at: 3292 3659 3292 Size: 367 5595 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2082 3283 4738 618 Size: 325 217 627 295 1201 1455 1842 Fragments arranged by size: 1842 1455 1201 627 325 295 217 BanII G_rGCy'C Cuts at: 1391 3281 1391 Size: 1890 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3032 928 Size: 971 1133 3858 Fragments arranged by size: 3858 1133 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4153 4187 1903 Size: 34 50 34 2132 34 3678 Fragments arranged by size: 3678 2132 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2459 3854 5064 2459 Size: 1395 1210 3357 Fragments arranged by size: 3357 1395 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5962 BfrBI ATG'CAT Cuts at: 2367 2367 Size: 5962 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2151 4573 Size: 107 122 71 631 605 337 142 2422 Cuts at: 4573 136 Size: 1525 Fragments arranged by size: 2422 1525 631 605 337 142 122 107 71 BglII A'GATC_T Cuts at: 5957 5957 Size: 5962 BlpI GC'TnA_GC Cuts at: 2762 3379 2762 Size: 617 5345 Bme1580I G_kGCm'C Cuts at: 1790 4023 5179 1790 Size: 2233 1156 2573 Fragments arranged by size: 2573 2233 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5962 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3693 4651 442 Size: 416 53 83 399 24 2276 958 1753 Fragments arranged by size: 2276 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2502 1056 Size: 1446 4516 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2020 2128 2692 3045 4622 1239 Size: 781 108 564 353 1577 2579 Fragments arranged by size: 2579 1577 781 564 353 108 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2862 4087 4952 5250 2862 Size: 1225 865 298 3574 Fragments arranged by size: 3574 1225 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4625 882 Size: 617 3126 2219 Fragments arranged by size: 3126 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3753 493 Size: 711 2549 2702 Fragments arranged by size: 2702 2549 711 BsaBI GATnn'nnATC Cuts at: 2180 3146 5956 2180 Size: 966 2810 2186 Fragments arranged by size: 2810 2186 966 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3769 4151 4786 275 Size: 53 83 186 936 2236 382 635 1451 Fragments arranged by size: 2236 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4395 4793 5067 5214 5625 4395 Size: 398 274 147 411 4732 Fragments arranged by size: 4732 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2018 2048 1178 Size: 30 165 30 615 30 5092 Fragments arranged by size: 5092 615 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3100 1186 Size: 1914 4048 BseYI C'CCAG_C Cuts at: 2431 3070 3098 5165 5661 2431 Size: 639 28 2067 496 2732 Fragments arranged by size: 2732 2067 639 496 28 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2262 1163 Size: 573 526 4863 Fragments arranged by size: 4863 573 526 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3412 4173 5201 1540 Size: 275 186 1411 761 1028 2301 Fragments arranged by size: 2301 1411 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4023 4108 5179 4023 Size: 85 1071 4806 Fragments arranged by size: 4806 1071 85 BsmI GAATG_Cn' Cuts at: 3473 3566 3473 Size: 93 5869 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3281 4023 4108 5179 1391 Size: 399 1491 742 85 1071 2174 Fragments arranged by size: 2174 1491 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5625 5625 Size: 5962 BspHI T'CATG_A Cuts at: 2545 3851 5581 2545 Size: 1306 1730 2926 Fragments arranged by size: 2926 1730 1306 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2094 3317 844 Size: 1250 1223 3489 Fragments arranged by size: 3489 1250 1223 BsrBI CCG'CTC Cuts at: 1394 1823 3849 1394 Size: 429 2026 3507 Fragments arranged by size: 3507 2026 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2766 4457 4631 65 Size: 1183 1518 1691 174 1396 Fragments arranged by size: 1691 1518 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4606 4793 1700 Size: 110 2796 187 2869 Fragments arranged by size: 2869 2796 187 110 BsrGI T'GTAC_A Cuts at: 96 3199 96 Size: 3103 2859 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5962 BssSI C'ACGA_G Cuts at: 2567 4022 5034 2567 Size: 1455 1012 3495 Fragments arranged by size: 3495 1455 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5962 BstBI TT'CG_AA Cuts at: 4766 4766 Size: 5962 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5962 BstYI r'GATC_y Cuts at: 1972 2317 3292 3659 4044 4061 5513 5599 5611 Size: 345 975 367 385 17 1452 86 12 Cuts at: 5611 5957 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 975 385 367 346 345 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5410 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5223 Fragments arranged by size: 5223 683 56 BtsI GCAGTG_nn' Cuts at: 3478 4272 4292 3478 Size: 794 20 5148 Fragments arranged by size: 5148 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5962 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2376 2411 529 Size: 35 1812 35 4080 Fragments arranged by size: 4080 1812 35 35 DraI TTT'AAA Cuts at: 3266 3266 Size: 5962 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4969 809 Size: 507 3653 1802 Fragments arranged by size: 3653 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2400 2481 3409 Size: 54 1425 50 275 18 570 81 928 Cuts at: 3409 4298 5927 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 928 889 570 275 81 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3409 1537 Size: 275 1597 4090 Fragments arranged by size: 4090 1597 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2586 2905 2931 3892 1353 Size: 1233 319 26 961 3423 Fragments arranged by size: 3423 1233 961 319 26 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4549 4923 5069 387 Size: 1064 138 2960 374 146 1280 Fragments arranged by size: 2960 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2020 2128 2610 2692 2852 2886 3045 Size: 393 388 108 482 82 160 34 159 Cuts at: 3045 4025 4622 5409 5677 1239 Size: 980 597 787 268 1524 Fragments arranged by size: 1524 980 787 597 482 393 388 268 160 159 108 82 34 EcoRI G'AATT_C Cuts at: 3271 3649 3271 Size: 378 5584 FspI TGC'GCA Cuts at: 4468 4468 Size: 5962 HaeII r_GCGC'y Cuts at: 1678 5109 1678 Size: 3431 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2018 2050 2224 2256 2585 Size: 32 135 32 446 32 174 32 329 Cuts at: 2585 2617 2701 2733 4603 4635 4677 4709 1373 Size: 32 84 32 1870 32 42 32 2626 Fragments arranged by size: 2626 1870 446 329 174 135 84 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3306 3552 669 Size: 988 281 1368 246 3079 Fragments arranged by size: 3079 1368 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5962 HpaI GTT'AAC Cuts at: 3552 3552 Size: 5962 KpnI G_GTAC'C Cuts at: 2086 3287 2086 Size: 1201 4761 MfeI C'AATT_G Cuts at: 3561 3561 Size: 5962 MluI A'CGCG_T Cuts at: 5838 5838 Size: 5962 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2913 3017 5076 5260 1305 Size: 1608 104 2059 184 2007 Fragments arranged by size: 2059 2007 1608 184 104 MscI TGG'CCA Cuts at: 10 64 1489 1832 2402 2483 10 Size: 54 1425 343 570 81 3489 Fragments arranged by size: 3489 1425 570 343 81 54 MslI CAynn'nnrTG Cuts at: 518 1166 2048 2366 2550 2753 3920 4279 4438 Size: 648 882 318 184 203 1167 359 159 Cuts at: 4438 518 Size: 2042 Fragments arranged by size: 2042 1167 882 648 359 318 203 184 159 MspA1I CmG'CkG Cuts at: 1606 2015 2582 3023 3378 4055 5203 5448 1606 Size: 409 567 441 355 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 567 441 409 355 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5410 NdeI CA'TA_TG Cuts at: 387 387 Size: 5962 NheI G'CTAG_C Cuts at: 1052 2498 1052 Size: 1446 4516 NotI GC'GGCC_GC Cuts at: 3409 3409 Size: 5962 NsiI A_TGCA'T Cuts at: 2369 2369 Size: 5962 NspI r_CATG'y Cuts at: 1132 1229 2367 3320 4865 1132 Size: 97 1138 953 1545 2229 Fragments arranged by size: 2229 1545 1138 953 97 PasI CC'CwG_GG Cuts at: 1348 3222 1348 Size: 1874 4088 PciI A'CATG_T Cuts at: 4861 4861 Size: 5962 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5962 PmeI GTTT'AAAC Cuts at: 3266 3266 Size: 5962 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2695 2727 4031 4063 5544 5576 2695 Size: 32 1304 32 1481 32 3081 Fragments arranged by size: 3081 1481 1304 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5962 PsiI TTA'TAA Cuts at: 3532 3532 Size: 5962 PstI C_TGCA'G Cuts at: 830 2842 3314 830 Size: 2012 472 3478 Fragments arranged by size: 3478 2012 472 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5962 PvuII CAG'CTG Cuts at: 2015 2582 3023 2015 Size: 567 441 4954 Fragments arranged by size: 4954 567 441 SalI G'TCGA_C Cuts at: 1936 3304 1936 Size: 1368 4594 SbfI CC_TGCA'GG Cuts at: 3314 3314 Size: 5962 ScaI AGT'ACT Cuts at: 4210 5922 4210 Size: 1712 4250 SfcI C'TryA_G Cuts at: 826 1045 2838 3310 4445 5126 5317 826 Size: 219 1793 472 1135 681 191 1471 Fragments arranged by size: 1793 1471 1135 681 472 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5962 SmaI CCC'GGG Cuts at: 1872 3289 1872 Size: 1417 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2877 4066 4967 5229 820 Size: 197 926 934 1189 901 262 1553 Fragments arranged by size: 1553 1189 934 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3753 493 Size: 3260 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5962 SphI G_CATG'C Cuts at: 1132 3320 1132 Size: 2188 3774 SspI AAT'ATT Cuts at: 5 52 3886 5 Size: 47 3834 2081 Fragments arranged by size: 3834 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5962 StyI C'CwwG_G Cuts at: 513 1065 3401 513 Size: 552 2336 3074 Fragments arranged by size: 3074 2336 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3989 4006 4159 4344 4789 325 Size: 1255 2409 17 153 185 445 1498 Fragments arranged by size: 2409 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3199 4208 5920 Size: 274 80 33 51 1443 1222 1009 1712 Cuts at: 5920 96 Size: 138 Fragments arranged by size: 1712 1443 1222 1009 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5922 5954 5922 Size: 32 5930 Tth111I GACn'n_nGTC Cuts at: 1316 3188 1316 Size: 1872 4090 XbaI T'CTAG_A Cuts at: 3298 3298 Size: 5962 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2491 2491 Size: 5962 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5962 XmaI C'CCGG_G Cuts at: 1870 3287 1870 Size: 1417 4545 XmnI GAAnn'nnTTC Cuts at: 4091 4091 Size: 5962 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3770 276 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI