Restriction map for
pFN21AB7306
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# NO CUT ENZYMES ( 110 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
AocI Aor51HI AscI AseI AsnI
Asp718I AspEI AviII AvrII BamHI
BanI BbeI BbvCI BcgI BclI
BfrI BglII BlnI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BsrBI BsrFI BssHII
Bst1107I BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
FauI FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HincII HindII
HpaI KasI KpnI KspI MfeI
MluI MroI MunI MvnI NaeI
NarI NgoMIV NheI NotI NruI
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I SspI SwaI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AlwNI 1690 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1681 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2377 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 3051 TGGCCA TGG^CCA
BbrPI 192 CACGTG CAC^GTG
BciVI 2313 GGATAC GTATCC(6/5)
BlpI 592 GCTGAGC GC^T[ACGT]AGC
BpmI 2277 CTCCAG CTGGAG(16/14)
Bpu1102I 592 GCTGAGC GC^T[ACGT]AGC
BsmI 405 GAATGC GAATGC(1/-1)
Bsp1407I 715 TGTACA T^GTACA
BspHI 3084 TCATGA T^CATGA
BsrGI 715 TGTACA T^GTACA
BssSI 1046 CACGAG CACGAG(-5/-1)
BstYI 2538 GGATCT [GA]^GATC[CT]
CelII 592 GCTGAGC GC^T[ACGT]AGC
DraIII 392 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 3051 TGGCCA [CT]^GGCC[GA]
EcoNI 2605 CCTGTTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 644 GAATTC G^AATTC
HgaI 564 GACGC GACGC(5/10)
MflI 2538 GGATCT [GA]^GATC[CT]
MluNI 3051 TGGCCA TGG^CCA
MscI 3051 TGGCCA TGG^CCA
MslI 1520 CACTTATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1980 CCATGG C^CATGG
PaeR7I 2366 CTCGAG C^TCGAG
PflFI 2377 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 733 CCAGTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 192 CACGTG CAC^GTG
PmlI 192 CACGTG CAC^GTG
PpuMI 418 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 3084 TCATGA T^CATGA
SacI 254 GAGCTC GAGCT^C
SspBI 715 TGTACA T^GTACA
StuI 1421 AGGCCT AGG^CCT
Tth111I 2377 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 733 CCAGTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1770 TCTAGA T^CTAGA
XhoI 2366 CTCGAG C^TCGAG
XhoII 2538 GGATCT [GA]^GATC[CT]
XmnI 1681 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AflIII 2317 ACATGT A^C[GA][CT]GT
2849 ACATGT
Alw44I 389 GTGCAC G^TGCAC
2809 GTGCAC
ApaI 1167 GGGCCC GGGCC^C
1377 GGGCCC
ApaLI 389 GTGCAC G^TGCAC
2809 GTGCAC
AvaI 2165 CTCGGG C^[CT]CG[GA]G
2366 CTCGAG
BcnI 416 CCGGG CC^[GC]GG
2385 CCGGG
BsaAI 192 CACGTG [CT]AC^GT[GA]
2183 TACGTG
BsaBI 2297 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2628 GATGCACATC
BsmAI 1888 GAGAC GTCTC(1/5)
1908 GAGAC
BsoBI 2165 CTCGGG C^[CT]CG[GA]G
2366 CTCGAG
BspLU11I 2317 ACATGT A^CATGT
2849 ACATGT
BsrDI 583 GCAATG GCAATG(2/0)
2525 GCAATG
BstBI 2555 TTCGAA TT^CGAA
2756 TTCGAA
HapII 416 CCGG C^CGG
2385 CCGG
HpaII 416 CCGG C^CGG
2385 CCGG
HphI 634 GGTGA GGTGA(8/7)
3043 GGTGA
MaeII 193 ACGT A^CGT
2184 ACGT
MaeIII 2375 GTGAC ^GT[ACGT]AC
3044 GTGAC
MamI 2297 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2628 GATGCACATC
MspA1I 2157 CAGCTG C[AC]G^C[GT]G
2453 CAGCTG
MspI 416 CCGG C^CGG
2385 CCGG
NciI 416 CCGGG CC^[GC]GG
2385 CCGGG
NspV 2555 TTCGAA TT^CGAA
2756 TTCGAA
PvuII 2157 CAGCTG CAG^CTG
2453 CAGCTG
SfuI 2555 TTCGAA TT^CGAA
2756 TTCGAA
SmlI 2366 CTCGAG C^T[CT][GA]AG
2927 CTCAAG
Tsp45I 2375 GTGAC ^GT[GC]AC
3044 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanII BbsI
BbvI BfaI BglI BmyI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BspMI BsrI
BssKI BstAPI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraI DraII
DsaI EarI EcoO109I EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HindIII
HinfI ItaI Ksp632I MaeI MboI
MboII MnlI MseI MvaI MwoI
NdeI NdeII NlaIII NlaIV NsiI
NspI PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SphI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XcmI